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REVIEW article

Front. Plant Sci., 03 June 2014
Sec. Plant Pathogen Interactions
This article is part of the Research Topic Plant cell wall in pathogenesis, parasitism and symbiosis View all 15 articles

Uncovering plant-pathogen crosstalk through apoplastic proteomic studies

  • Laboratoire Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne-EA 4707, Université de Reims Champagne-Ardenne, Reims, France

Plant pathogens have evolved by developing different strategies to infect their host, which in turn have elaborated immune responses to counter the pathogen invasion. The apoplast, including the cell wall and extracellular space outside the plasma membrane, is one of the first compartments where pathogen-host interaction occurs. The plant cell wall is composed of a complex network of polysaccharides polymers and glycoproteins and serves as a natural physical barrier against pathogen invasion. The apoplastic fluid, circulating through the cell wall and intercellular spaces, provides a means for delivering molecules and facilitating intercellular communications. Some plant-pathogen interactions lead to plant cell wall degradation allowing pathogens to penetrate into the cells. In turn, the plant immune system recognizes microbial- or damage-associated molecular patterns (MAMPs or DAMPs) and initiates a set of basal immune responses, including the strengthening of the plant cell wall. The establishment of defense requires the regulation of a wide variety of proteins that are involved at different levels, from receptor perception of the pathogen via signaling mechanisms to the strengthening of the cell wall or degradation of the pathogen itself. A fine regulation of apoplastic proteins is therefore essential for rapid and effective pathogen perception and for maintaining cell wall integrity. This review aims to provide insight into analyses using proteomic approaches of the apoplast to highlight the modulation of the apoplastic protein patterns during pathogen infection and to unravel the key players involved in plant-pathogen interaction.

Introduction

Higher plants interact continuously with microbes such as viruses, bacteria, oomycetes or fungi, some of which are phytopathogens, leading to plant diseases. The lifestyle of the pathogen determines the nature of this interaction (Doehlemann and Hemetsberger, 2013). Biotrophic pathogens have developed specific strategies to interact with the cell wall and keep plant cells alive during their life cycles. In contrast, necrotrophic pathogens feed on dead plant cells. Plants naturally display preformed defenses, which include the cell wall and cuticle acting as a physical barrier. However, these preformed defenses are sometimes not strong enough to stop the invading pathogen. Successful resistance then comes from a rapid activation of the plant's innate immune system (Boller and Felix, 2009). Plant perception of conserved molecules characteristic of many microbes is the first step in this innate immune response. These molecules, more commonly called general elicitors, are also referred to microbe-associated molecular patterns (MAMPs) (Jones and Dangl, 2006; Pel and Pieterse, 2013). MAMPs are recognized by pattern recognition receptors (PRRs), which are generally localized at the level of the plasma membrane. MAMP perception leads to the establishment of the so-called MAMP-triggered immunity (MTI) (Boller and Felix, 2009). The small peptide flg22 derived from bacterial flagellin and the elongation-factor Tu peptide elf18 are the most extensively studied MAMPs (Felix and Boller, 2003; Zipfel, 2009; Trdá et al., 2013). Many other MAMPs have been identified (reviewed in Newman et al., 2013) such as eicosapolyenoic acids (Bostock et al., 1981; Savchenko et al., 2010), β-glucans (Umemoto et al., 1997; Klarzynski et al., 2000), peptidoglycans (Willmann et al., 2011), lipopolysaccharides (Newman et al., 2002; Erbs and Newman, 2012), rhamnolipids (Varnier et al., 2009; Sanchez et al., 2012), or chitin oligomers (Kaku et al., 2006; Miya et al., 2007). Pathogens can suppress MTI by secreting effector proteins that act either by inhibiting the MAMP-PRR interaction or downstream signaling. The direct or indirect recognition of effectors (previously called specific elicitors) by plant resistance gene products results in the so-called effector-triggered immunity (ETI) (Pel and Pieterse, 2013). ETI is usually quantitatively stronger than MTI and associated with more sustained and robust immune responses including localized cell death (HR, hypersensitive response) (Tsuda and Katagiri, 2010). Some plant-derived molecules called damage-associated molecular patterns (DAMPs) are also recognized by the plant itself and can trigger an immune response (Boller and Felix, 2009). The well-known systemin or oligogalacturonides released upon cell wall damage were shown to act as DAMPs (Pearce et al., 1991; Schweizer et al., 1996; Denoux et al., 2008; Brutus et al., 2010; Ferrari et al., 2013). Recently, the plant endogenous peptides AtPeps have also been characterized as powerful DAMPs (Yamaguchi and Huffaker, 2011).

Induction of defenses by MAMPs, DAMPS or effectors starts within minutes after signal perception with ion fluxes, MAPK kinase activation, the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) such as nitric oxide (NO) (Garcia-Brugger et al., 2006; Scheler et al., 2013). ROS and NO can act in signaling and have direct antimicrobial effects. ROS are also involved in plant cell wall strengthening by oxidative cross-linking of polymers. During the plant immune response, the phytohormones salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) play a key role in signal transduction (Robert-Seilaniantz et al., 2011). The importance of JA and SA as primary signals in the regulation of plant immune responses has been well established (Robert-Seilaniantz et al., 2011; Pieterse et al., 2012). The JA pathway is primarily induced by and effective in mediating resistance against necrotrophic pathogens, whereas the SA pathway is primarily induced by and effective in mediating resistance against biotrophic pathogens (Glazebrook, 2005). Nonetheless, this is an over-simplistic view of disease resistance mechanisms as there are complex repertoires of plant hormones that play a role in defense signaling pathways. Indeed, other hormones such as auxin, abscisic acid, cytokinins, and brassinosteroids function as modulators of the plant immune signaling network (Robert-Seilaniantz et al., 2011; Pieterse et al., 2012). Not surprisingly, pathogenic microbes have developed strategies to manipulate plant hormonal pathways in order to divert the immune signaling mechanism for their own benefit. Recent studies suggest that these pathogen-induced modulations of signaling pathways via hormones contribute to virulence (Pieterse et al., 2012). Therefore, the complex crosstalk and induced hormonal changes modulate disease and resistance with the outcomes dependent on pathogen lifestyles and the genetic constitution of the host (Robert-Seilaniantz et al., 2011; Gimenez-Ibanez and Solano, 2013). Thus, pathogens have adapted different types of complex interactions. Biotrophs depend on the living plant metabolism as their nutritional source, and therefore interact intimately with the host cells to modify metabolic processes (Glazebrook, 2005; Horbach et al., 2011). Necrotrophs, on the other hand, invade and kill the plant tissue, feeding on the dead tissue debris. To this end, they usually macerate plant tissues by secreting toxins and abundant hydrolytic enzymes that degrade cell wall polymers (Laluk and Mengiste, 2010). As for the plant, defense responses occur upon pathogen recognition through reinforcement of the plant cell wall to counter pathogen invasion, while production of antimicrobial compounds such as phytoalexins (Jeandet et al., 2013) and synthesis of pathogenesis-related (PR) proteins including hydrolytic enzymes like β-1,3-glucanases and chitinases (Van Loon et al., 2006) contribute to the alteration of pathogen integrity.

Studies of plant-pathogen interactions are numerous in the literature and include a wide range of physiological, molecular and biochemical approaches. Proteomics has become an important tool for large-scale analysis of the proteins involved in the complex plant-pathogen interactions in the post-genomic area (for review see Quirino et al., 2010; Jayaraman et al., 2012). Characterization of a set of proteins under specific plant-pathogen interactions provides a more direct view of cellular processes than DNA or RNA analysis. Proteomics provides insight into protein localization, protein–protein interactions, enzymatic complexes or post-translational modifications that are essential to a better understanding of plant-pathogen interactions. Proteomic approaches have been used in recent years to further characterize plant interactions with viruses (Casado-Vela et al., 2006; Giribaldi et al., 2011; Li et al., 2011; Di Carli et al., 2012), bacteria (Jones et al., 2006; Afroz et al., 2009, 2013; Li et al., 2012), or fungi (Kim et al., 2004a; Geddes et al., 2008; Bhadauria et al., 2010; Mukherjee et al., 2010; Shah et al., 2012). The general picture of changes occurring in the plant-host proteome highlights common features among the broad range of interaction analyzed. A common response, observed in almost all studies, is related to plant photosynthetic activity, which is negatively regulated by pathogen infection, most probably reflecting allocation of energy resources to a general plant defense regulatory mechanism. In parallel, plants counteract to pathogen infection by modulating the accumulation of defense- or stress-associated proteins and proteins involved in ROS metabolism. However, most of the key proteins involved in the plant-pathogen interaction are probably produced at low levels and the majority of studies only detected the most abundant pathogen protein, such as coat protein for virus. These global approaches also present significant technical challenges, as they generally need to differentiate between plant and pathogen proteins (Mathesius, 2009). Simplified models have been developed to circumvent these technical challenges. In some studies, the plant was treated with a MAMP from a pathogen (Chivasa et al., 2006; Liao et al., 2009) or with a signal molecule (Rajjou et al., 2006; Macarisin et al., 2009) to characterize the proteomic changes within the plant. Other studies focused on the pathogen secretome alone (Brown et al., 2012; Girard et al., 2013) or in the presence of plant extracts (Phalip et al., 2005; Fernandez Acero et al., 2009). However, the absence of one of the actors in these simplified models underestimates the complexity of the events occurring during the plant-pathogen crosstalk. The large dynamic range of protein abundance present in plant-pathogen samples, such as pathogen-infected leaves represents an additional difficulty (Bindschedler and Cramer, 2011). In fact, many important proteins are present at low level and are thus difficult to isolate from complex mixtures containing more highly abundant proteins. As the resolution of analytical separation methods is too limited to dissect the total proteome of a cell or a tissue, less abundant proteins are often masked by those produced at higher levels. Sub-cellular proteomics has the advantage not only of relating proteins to a functional compartment of eukaryotic cells, but also of reducing the complexity of the whole cell or tissue protein extracts (Brunet et al., 2003). However, the isolation of sub-cellular proteins typically requires a series of labor-intensive steps. Thus, efficient protocols for sub-cellular fractionation, purification, and enrichment are necessary for each cellular compartment (Lee et al., 2013).

Important processes such as development, intercellular communications or defense mechanisms take place in the apoplast (Sakurai, 1998). The apoplastic proteins are involved in different physiological and biological processes related to growth regulation, biotic and abiotic stresses and cell wall maintenance (Ellis et al., 2007; Tseng et al., 2009). The apoplast or apoplastic space is one of the first physiological compartments of pathogen-host exchanges and the key processes that occur there during microbial infections therefore determine the fate of the interaction (Doehlemann and Hemetsberger, 2013). The apoplast is defined as the extracellular matrix or plant cell wall and the intercellular spaces where the apoplastic fluid circulates (Agrawal et al., 2010). The apoplastic fluid plays a key role in intercellular and intracellular communications and is composed of many substances, notably nutrients, polysaccharides, secondary metabolites and secreted proteins. Lohaus et al. (2001) showed low metabolite concentrations in the apoplastic solution from Vicia faba, Spinacia oleracea, Hordeum vulgare, and Zea mays. The sucrose concentration was about 1–2 mM in all plant species, whereas the concentration of hexoses differed strongly between the species. Similarly, the highest concentration of amino acids in the apoplastic solution was found in Vicia faba (about 10 mM), the lowest in Zea mays (about 2 mM). It is also well known that the redox and pH control in the apoplast serves as a mechanism to respond to environmental signals. The main cation in the apoplastic solution of the analyzed plant species was potassium, representing about 70–80% of the total cation concentration when the main anions were nitrate and chloride. In Spinacia oleracea, oxalate was also an important anion, while apoplast from Hordeum vulgare contained high amounts of malate. The charge balance was equal and total contents of cations and anions were between 10 and 20 mM, respectively. The activity of plasma membrane-bound H+-ATPase and membrane transport of solutes determine the pH condition of apoplast but pectic substances in the cell walls also affect the ion concentrations and pH in apoplast. The measured pH range of apoplast by pH electrode in apoplast of different plants varies from 4.5 to 7 (Sakurai, 1998).

The plant cell wall is mainly composed of polysaccharides such as celluloses, hemicelluloses and pectins, which interlock to form a dense and complex network. Additional compounds such as lignins, waxes or cutins are synthesized to form the secondary wall in specific differentiated cells (Carpita and Gibeaut, 1993). The cell wall acts as a passive barrier limiting the access of pathogens to plant cells and in turn, pathogens, especially necrotrophs, actively synthesize cell wall-degrading enzymes to penetrate or kill the plant cell (Laluk and Mengiste, 2010). The plant cell wall is actively remodeled and reinforced specifically at discrete sites of interaction with pathogens (Hamann, 2012; Underwood, 2012). This active reinforcement through the deposition of cell wall appositions, referred to as papillae, is one of the earliest responses to pathogen attacks (Micali et al., 2011). However, the cell wall proteome under a biotic stress is still poorly characterized. Identifying the proteins present in the apoplast during a pathogen infection is therefore essential to understanding the perception and regulation processes occurring between the two protagonists. A proteomic analysis that provides an overview of the protein pool at a given time is thereby an appropriate tool to address this issue and identify the actors involved in the interaction. Through this review, we will begin by pointing out the technical constraints to recovering apoplastic samples for proteomic analyses. We will then highlight the major findings obtained from the apoplastic proteome patterns during plant-pathogen interactions. In perspective, we will suggest future approaches to characterize early protein interconnections taking place between the pathogen and its host in the apoplast.

Secretome and Apoplastic Proteome Isolation

Despite the importance of apoplastic proteome during a given plant-pathogen interaction, it remains poorly characterized compared to the intracellular proteome. This is especially due to the difficulty in obtaining sufficient apoplastic material without damaging the plant cell and in avoiding potential contamination of the sample with cytoplasmic proteins. The proteins secreted in the apoplast are either soluble in the apoplastic fluid or ionically bonded to the plant cell wall (Soares et al., 2007). The literature uses different terms for the extracellular proteome present in the apoplast, the most common being “secretome” and plant “cell wall proteome.” For clarity and convenience, in this review, we will use the term “secretome” to designate secreted proteome obtained from in vitro cell suspension cultures, “apoplastic proteome” for soluble proteins present in the apoplastic fluid (generally extracted by the VIC method discussed below) and “cell wall proteome” for the secreted proteins that are loosely ionically bonded to the cell wall. The cell wall proteome is generally obtained from purified cell walls produced by disruptive methods. The cell wall proteome has been well investigated and excellent reviews have been published recently (Jamet et al., 2008; Rose and Lee, 2010; Albenne et al., 2013; Komatsu and Yanagawa, 2013).

Several studies have used suspension cell cultures such as in vitro-simplified models to develop practical, simple and non-destructive methods to isolate secreted proteins. The suspension cell cultures are easy to maintain, handle and scale up/down and the secreted proteins in the culture medium can be easily separated from suspended cells by filtration without cell disruption. Therefore, this system facilitates the extraction of freely soluble secreted proteins by the plant cells in suspension cultures and greatly limits potential contamination by cytoplasmic proteins. This simplified approach has been used to characterize basal secretomes of different species like Arabidopsis (Oh et al., 2005), alfalfa (Kusumawati et al., 2008), tobacco (Okushima et al., 2000), or rice (Cho et al., 2009). These in vitro systems have been used to assess cell responses more easily following signal molecules or fungal-derived elicitor treatments (Table 1). The effect of the well-known signal molecules SA and JA was characterized on Arabidopsis and grapevine cell suspension secretomes, respectively (Oh et al., 2005; Cheng et al., 2009; Martinez-Esteso et al., 2009). Comparative secretome studies have been performed with chitosan and Fusarium-based elicitors on Arabidopsis and maize (Ndimba et al., 2003; Chivasa et al., 2005). These studies revealed proteome changes in response to individual MAMPs but without taking into account the complexity of the responses triggered during a typical plant-microorganism interaction. To our knowledge, only two studies have directly used the pathogen itself, demonstrating that the study of plant-pathogen interactions has proven to be very difficult in these in vitro systems (Kaffarnik et al., 2009; Kim et al., 2009). Even then, the in vitro secretome analysis only provides partial identification of the secreted proteins in comparison to the in planta apoplastic proteome analysis as demonstrated by the comparative analysis of in vitro secretome and leaf apoplastic proteomes in rice (Jung et al., 2008). Analyses of whole secreted proteins identified 222 protein spots with only 6 protein spots common to both in planta and in vitro samples. The proteins involved in cell wall metabolism in relation with plant defense mechanisms represent 18% of the total proteins identified in planta compared to 64% in vitro.

TABLE 1
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Table 1. Main secretome studies on in vitro plant cell suspension under an elicitor or pathogen treatment.

Since the in vitro approach does not provide a natural environment for the cells and because physiologically relevant treatments are difficult to apply, recent studies were instead carried out in planta. In this case, when a plant organ such as a leaf is required for study, apoplastic fluid is most commonly isolated using the vacuum infiltration centrifugation (VIC) method, well described by Lohaus et al. (2001) and Agrawal et al. (2010). In short, the plant leaves are thoroughly rinsed with a buffer to reduce the leaf surface tension and facilitate the vacuum infiltration. After infiltration with the adapted extraction buffer, the leaf surfaces are quickly dried to avoid sample dilution and the carefully rolled leaves are transferred to 50 ml polypropylene tubes with a washer at the bottom to avoid immersion of leaves into the collected apoplastic fluid. The gentle centrifugation allows the recovery of the apoplastic fluid from which apoplastic proteins are extracted by precipitation. The VIC method has been used to characterize the apoplastic proteome of different plant species such as Arabidopsis (Casasoli et al., 2008), rice (Cho et al., 2009), tobacco (Delannoy et al., 2008), maize (Witzel et al., 2011), alfalfa (Soares et al., 2007), pea (Wen et al., 2007), tomato (Konozy et al., 2012), or grapevine (Delaunois et al., 2013). This VIC method has also been used to assess the apoplastic proteome changes occurring after an elicitor, a pathogenic or non-pathogenic treatment (Table 2). Casasoli et al. (2008) have used oligogalacturonides on Arabidopsis to identify apoplastic candidate proteins involved in the response to this elicitor and the proteins associated with H2O2 response was investigated in the rice root apoplast (Zhou et al., 2011). Apoplastic proteome changes were studied during plant interactions with pathogenic bacteria (Agrobacterium tumefaciens and Pseudomonas syringae) and fungal pathogens, mainly Verticillium longisporum and Magnaporthae oryzae (Table 2). However, methodological adaptations might be necessary for efficient sample preparation. For instance, a water-displacement method was developed to obtain apoplast fluids from stem tissues in the poplar/Melampsora medusa interaction (Pechanova et al., 2010). The VIC technique allows the apoplastic proteome extraction without much cell damage. However, at times, the fragility of the samples leads to the rupture of the cytoplasmic membrane, triggering varying contamination by cytoplasmic proteins. The apoplastic fluid sample requires more stringent assessment of intracellular contamination to ensure apoplastic fraction purity. To estimate cytoplasmic contamination, enzyme activity, immunoblotting or electrolyte leakage can be used. Malate dehydrogenase activity is the most commonly used measure to estimate the level of membrane damage caused by the VIC method. Antibodies directed against malate dehydrogenase, RuBisCo or ATPase are also frequently used to determine the contamination level (Delaunois et al., 2013). Electrolyte leakage and concentration of malondialdehyde, which is a breakdown product of membrane lipid peroxidation, can also be used (Zhou et al., 2011).

TABLE 2
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Table 2. Main apoplastic proteome studies after an elicitor or pathogen treatment.

Protein Separation, Identification, and Quantification Techniques

The main problem with the VIC technique is the extremely low yield implying that either very large volume of samples have to be produced and concentrated or very sensitive methods have to be used for proteomic analysis. A significant amount of information has been gained from proteomic studies using classical gel-based separation, as resolved proteins can often be identified and further characterized by mass spectrometry (MS). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) involves resolving proteins by isoelectric point (pI) and molecular weight (Görg et al., 2004; Kav et al., 2007). The 2D-PAGE has mainly been used for identifying plant protein abundance alterations in secretome or apoplastic proteomes in response to biotic stresses and still remains a viable technique (Shenton et al., 2012). Fluorescence difference gel electrophoresis (DIGE) was developed as a more quantitative form of 2D-PAGE. Here, samples are differentially covalently labeled with fluorophores, allowing for distinction between proteins resolved on the same gel (Casasoli et al., 2008). Casasoli et al. (2008) used this technique in Arabidopsis to identify the differential expression of 62 proteins in the same gel between control and oligogalaturonide-treated apoplastic proteomes. Both 2D-PAGE and DIGE approaches control for gel-to-gel variation, but do not overcome the issues of spot overlap (Campostrini et al., 2005). The gel-free methods utilizing liquid chromatography (LC) techniques for separating peptides after sequence-specific digestion can overcome this issue and significantly increase proteome coverage (Roe and Griffin, 2006). For example, multidimensional protein identification technology (MudPIT), which combines strong cation exchange with reversed phase chromatography, has been used on rice leaves infected with the fungus Magnaporthe oryzae (Kim et al., 2013). During this interaction, over 730 secretory proteins were identified in the apoplast by combining 2D-PAGE and MudPIT techniques, 40% and 60% of these corresponding to rice and Magnaporthe oryzae, respectively. Increasingly, gel-based, and gel-free separation methods are used together as complementary techniques to increase the number of identified proteins (De-La-Pena et al., 2008; Cheng et al., 2009; Pechanova et al., 2010; Kim et al., 2013).

Protein identification is performed by mass spectrometry (MS) analysis. The three main steps are protein or peptide ionization, ion separation and detection. Matrix assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) are the two main ionization techniques that are applied in apoplastic proteome studies while ion separation and detection uses mainly time-of-flight (TOF) or quadrupole mass analyzer. Tandem MS (MS/MS) is now commonly used to improve the sensitivity and accuracy of peptides/proteins identification and the different techniques are very often combined (like MALDI-TOF/MS or ESI-MS/MS, Tables 1, 2). The identification of proteins present in the apoplast in a given plant-pathogen interaction implies an access to a proteome and/or a genome database of the two organisms, which is not always the case. Alexandersson et al. (2013) suggest the use of a combined plant-pathogen protein database extended with a random sequence database to avoid false positive hits from host peptides when matching pathogen peptides.

Plant-pathogen interaction mechanisms involve the fine modulation of protein amount. Precise and sensitive quantification methods of proteins become essential. Staining on polyacrylamide gels with Coomassie blue or silver nitrate is generally performed for spot quantification. However, quantifications on stained spots are difficult to interpret for several reasons: overlapping spots can occur, different proteins can be present in the same spot or some proteins can be present in different spots due to post-translational modifications (PTM), and degradation or maturation of proteins. The labeling of proteins with fluorescent dyes prior to electrophoresis (DIGE) can partially overcome some of these issues (Casasoli et al., 2008). More recently, protein quantification was significantly improved in proteomics using in vitro chemical (ICAT or iTRAQ) or in vivo metabolic (SILAC or 15N-labeling) isotope-assisted quantification methods. For in vivo metabolic stable isotope labeling, cell suspension cultures or plants are grown on media supplemented with heavy isotope-containing amino acids or 15N-labelled nutrients, allowing for the labeling of proteins as they are synthesized (Jayaraman et al., 2012). However, this approach is not always easy to implement in planta and requires long and powerful bioinformatics analysis. Kaffarnik et al. (2009) analyzed the secretome of Arabidopsis in response to infection by Pseudomonas syringae using a recently developed technique known as isobaric tag for relative and absolute quantification (iTRAQ). In this method, labeling is chemically performed in vitro on amines of digested peptide samples with commercially available iTRAQ (isobaric tags) reagents. The major advantage is that this strategy can be applied to directly compare up to eight separate samples in one experiment. With this technique Kaffarnik et al. (2009) compared apoplastic proteomes of Arabidopsis infected with three different strains of Pseudomonas syringae p.v. tomato, strain DC3000 (virulent), strain DC3000 carrying AvrRpm1 (avirulent) and strain DC3000 knocked-out for HrpA (non-pathogenic), suggesting a pathogen-mediated manipulation of apoplastic proteins. The development and the more systematic application of these isotope-assisted quantification and gel-free methods should allow the identification of low-abundance apoplastic proteins or small variations in their level of expression in the near future.

Leaderless Secretion Proteins

Plant proteins are secreted to the apoplast mainly via the classical ER-Golgi route. SignalP or TargetP software is widely used to predict signal peptides from the sequences (Emanuelsson et al., 2007; Petersen et al., 2011) and to identify proteins that are secreted through the classical ER-Golgi. However, there is increasing evidence that a subset of apoplastic proteins is likely to be secreted by non-classical pathways. Non-classical or leaderless secretion is common to all eukaryotes, including plants. Computational analysis using different algorithms have been developed to assist in the identification of unexpected secreted proteins. SecretomeP allows secretion prediction based on sequence features conserved or frequently observed in secreted bacterial and mammalian proteins (Bendtsen et al., 2004). Cheng et al. (2009) found that 60% of the Leaderless Secreted Proteins (LSPs) identified in the Arabidopsis secretome were predicted to be secreted with SecretomeP. Among these predicted LSPs actually found in the apoplast, we can mention calmodulin, jacalin, or superoxide dismutase. Although no identified plant superoxide dismutase has a signal peptide, extracellular superoxide dismutase activity in stressed or pathogen-infected plants has been previously reported (Hernández et al., 2001; Karpinska et al., 2001). In the same way, calmodulin is known to be an intracellular calcium sensor, but it has recently been suggested that calmodulin could serve as a dual messenger with roles either inside or outside the cell depending on stress factors (Cui et al., 2005). However, it should be noted that SecretomeP software may not be well-adapted to plant proteins since it has been designed for mammalian proteins. In addition, only a small proportion of the LSPs identified in apoplastic proteome studies gave a score above threshold (Cheng et al., 2009; Fernandez et al., 2012). The difficulties in preserving membrane integrity and extracting non-contaminated apoplastic fluids combined with the limitations of the bioinformatics programs in predicting sub-cellular localization have to be taken into account to understand the contrasting variations of LSPs content between experiments. New computational tools such as software or databases are emerging and should help to predict more precisely and with greater certainty the proteins produced through alternative secretion pathways. LocTree3 is a new software program that predicts protein subcellular localization through a consistent new framework with a high prediction success especially for secreted proteins (https://rostlab.org/services/loctree3) (Goldberg et al., 2012). The comparative platform OrysPSSP is composed of a core “small secreted protein” (SSP) database and a dynamic web interface that integrates a variety of user tools and resources and allows the screening of SSP on the genome scale and across the phylogeny of plant species (http://www.genoportal.org/PSSP/index.do) (Pan et al., 2013).

The existence of these alternative secretory routes could be explained by the need of rapid and effective regulation of secretion to provide a selective advantage to the plant cell. There is growing evidence of complex and highly coordinated spatiotemporal protein secretion in plants. Kaffarnik et al. (2009) showed that most of the proteins induced in Arabidopsis by the virulent Pseudomonas syringae DC3000 or the avirulent AvrRpm1 strains had no secretion signals as compared to non-pathogenic HrpA strains. Cheng et al. (2009) showed in Arabidopsis cell-suspensions treated with SA that 65%, 50%, and 35% of the secreted proteins lack a peptide signal after 1, 2, and 6 h, respectively. These results suggest that external stresses rapidly induce enhanced protein secretion. Other explanations could be the accumulation of inactive pre-proteins prior elicitation or posttranslational modifications made by the ER environment, which may not be required for specific activity of apoplastic proteins (Rose and Lee, 2010).

Post-Translational Modifications

Proteomic studies lead very often to the identification of the same protein in different spots suggesting different post-translational modifications (PTMs) of the same protein (Chivasa et al., 2005). Indeed, PTMs are known to control many physiological processes by affecting protein structure, activity, and stability. Proteins can undergo different PTMs such as glycosylation, phosphorylation, carbonylation, or nitrosylation (Jayaraman et al., 2012; Albenne et al., 2013). The secretome analysis of Arabidopsis cell suspensions in response to oligogalacturonides highlighted several protein isoforms, such as an alpha xylosidase and a receptor-like kinase, showing differential PTMs (Casasoli et al., 2008). This observation may suggest a role for PTMs in the plant response to pathogens. Glycosylation is one of the most common and complex PTM. There are two main types of glycosylation, namely N- and O-glycosylation, but plant glycoproteins still remain poorly characterized. Glycoproteomics are currently applied to plants (Albenne et al., 2013). ConA lectin chromatography approaches were used to specifically isolate N-glycoproteins from Arabidopsis (Minic et al., 2007) and tomato (Català et al., 2011). The use of a multi-dimensional lectin chromatography system increased the coverage of the Arabidopsis cell wall glycoproteome leading mainly to the identification of N-glycosylated proteins (Zhang et al., 2011). The regulation of enzymes putatively involved in glycosylation, such as the disappearance of a β − N − acetylglucosaminidase in the elicitor-treated maize secretome (Chivasa et al., 2005), suggests that glycosylation might also occur in the apoplast. The modulation of post-translational glycosylation would quickly regulate the activity and/or structure of targeted proteins, potentially strengthening the cell wall through stronger cross-linking.

Phosphorylation plays a key role in signal transduction and is based on the reversible regulation of the transfer of a phosphoryl group bonded to an aminoacid by protein kinases or removed by phosphatases. Although different phosphospecific staining techniques were developed for phosphoproteomic studies, LC-MS/MS analysis following gel-free separation and phosphopeptide enrichment is often the method of choice (Grimsrud et al., 2010). Upon perception of microbial signals, kinases and phosphatases target specific proteins, often modifying complex signaling cascades that allow for rapid defense responses. Ndimba et al. (2003) have shown that chitosan treatment of Arabidopsis cell-suspensions induce phosphorylation of a receptor-like kinase, endochitinases and glucanases. Similarly, Chivasa et al. (2005) have positively identified in the maize secretome phosphotyrosine protein spots that are rapidly dephosphorylated in response to Fusarium-elicitor treatment. The presence of elicitor-induced changes in the phosphorylation status of extracellular proteins suggests the existence of pathogen-induced, phosphorylation/dephosphorylation-regulated intercellular signaling via the extracellular matrix. Moreover the presence of phosphatases in the extracellular proteome of Arabidopsis infected by Pseudomonas syringae suggests that potential phosphorylation/dephosphorylation reversible regulation could occur in the apoplast (Kaffarnik et al., 2009). A recent comparison of Lotus japonicus roots elicited with symbiotic-(Nod factors) and the MAMP flg22 revealed differential phosphorylated protein patterns between symbiotic and defense responses (Serna-Sanz et al., 2011).

Carbonylation is considered as a marker of protein oxidation, which results from the direct oxidation of various aminoacids. This PTM is involved in the control of the protein function and can lead to their degradation (Lounifi et al., 2013). The early oxidative burst in response to pathogen attacks is leading to an increase of protein carbonylation (Zhang et al., 2007). Although there is a strong link between ROS and pathogen attack on one side and the ROS and protein carbonylation on the other side, so far no large-scale study has been conducted on the regulation of protein carbonylation during a given plant-pathogen interaction. As mentioned before, analysis of the apoplastic proteome under biotic stress has revealed an important part of the proteome changes involved in the ROS metabolism. This correlation suggests that large changes in ROS metabolism-related proteome in the apoplast would influence the redox balance and consequently the protein carbonylation level. The resulting rapid PTM activates other defense-related proteins.

Protein nitrosylation is considered as one of the key mechanism regulating protein function (Lounifi et al., 2013). Since nitrosylation refers to the covalent bonding of a NO molecule to the cysteine amninoacid it becomes apparent that NO species produced upon plant pathogen interactions can exert their signaling action through nitrosylation of specific proteins (Corpas et al., 2008; Spoel and Loake, 2011). A large-scale proteomic study conducted on Arabidopsis leaves treated with gaseous NO led to the identification of 25 nitrosylated proteins which are involved in stress response, redox status, signaling, and cytoskeleton functional categories. Pseudomonas syringae infection of Arabidopsis seedlings leads to a hypersensitive response accompanied by an NO burst and triggers an increase of nitrosylated proteins. Most of the identified nitrosylated proteins were enzymes involved in intermediary metabolism, signaling, and antioxidant defenses (Romero-Puertas et al., 2008). Therefore the extent of protein nitrosylation could be expected to change in response to NO-originated stimuli governed by pathogen infection. Moreover, the occurrence of a biological connection between protein oxidation and nitrosylation in plants appears to be increasingly documented (Lin et al., 2012). Since the ROS-based protein carbonylation and the NO-based protein nitrosylation, as well as their interactions, seem to act as major regulatory systems in stress responses, the characterization of protein oxidation and nitrosylation in plant-pathogen interactions becomes crucial to the understanding of the various physiological processes occurring in the apoplast. Plant PTM proteomics is still in its early stages and is undoubtedly a promising approach to gain new insights into the structure and function of apoplastic proteins during pathogen infection.

Main Findings from Apoplastic Proteomics Case Studies

Perception and Signal Transduction

As mentioned before, early perception of the pathogen occurs in the apoplastic compartment and several proteomic studies have highlighted the regulation of apoplastic proteins potentially involved in pathogen perception and signal transduction. These apoplastic proteins generally feature LRR-type motifs suggesting a potential receptor role for these proteins and they sometimes undergo PTM like phosphorylation suggesting an involvement in signal transduction cascades. In the secretome of Arabidopsis suspension cultures elicited with chitosan, the phosphorylation of a cell wall bound putative receptor-like protein suggests that elicitor-treatment involves signal transduction cascades initiated in the apoplast through accumulation of phosphorylated extracellular receptor-like proteins (Ndimba et al., 2003). In the apoplast of Arabidopsis leaves elicited by oligogalacturonides, Casasoli et al. (2008) observed the accumulation of a disease resistance related LRR protein, characterized by a LRR domain composed of 13 repeats of the extracytoplasmic type (eLRRs), and previously localized in the plant cell wall (Borderies et al., 2003; Bayer et al., 2006) (Figure 1). The correlation of this LRR protein accumulation with the induction of its corresponding transcript as well as the reported induction of its gene during the incompatible interaction with Alternaria brassicicola suggest a role in pathogen perception (Schenk et al., 2003).

FIGURE 1
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Figure 1. Schematic overview of some events occurring in apoplast during plant pathogen interactions. This illustration, based on studies described in this paper, presents some examples of apoplastic proteins regulated during biotic stresses. These proteins are secreted by the plant cell (green square) and/or pathogen (brown square). Some proteins, modulated in the apoplast after DAMP or MAMP perception, are involved in pathogen perception and signal transduction, leading to the activation of intracellular plant defense signaling pathways (blue arrows). The regulation of a large amount of proteins involved in redox homeostasis modulates the ROS signaling pathway leading to activation of extra- and intracellular plant defense responses (blue arrows). These proteins, such as peroxidases or oxalate oxidases participate also to the plant cell defense through plant cell wall reinforcement or direct pathogen attack (gray arrow). Plant cell wall is actively remodeled and/or reinforced through the regulation of numerous enzymes, such as polyglacturonases or glucanases, which are secreted by the pathogen or its host. Some of the cell wall degradation products can act as DAMP (green arrow) to stimulate plant defense signaling pathways. Most of the defense-related proteins, such as chitinases or glucanases, act directly against the pathogen (gray arrow), releasing degradation products that can be perceived as MAMP by the plant cell (brown arrow).

Receptor-like kinases with DUF26 domains are another class of interesting proteins related to biotic stress, regulated at both the protein and the transcript level, but with still unknown functions. DUF26 genes were previously found to be up-regulated upon pathogen infection, JA treatment (Kim et al., 2003, 2004b) and wounding (Shen et al., 2003). Recent studies have identified DUF26 proteins regulated upon pathogen infection or elicitor treatment (Kim et al., 2009, 2013; Zhou et al., 2011; Shenton et al., 2012). Several lines of evidence support the fact that these proteins could be involved in the perception of the pathogen or the transduction of the signal after perception. The proteins containing DUF26 domains, which are usually found in serine/threonine kinases, are annotated as small secretory proteins and are associated with plant receptor protein kinase domains in databases. These proteins accumulate earlier in incompatible rice/Magnaporthe grisae interactions than in compatible ones (Kim et al., 2009). Their extremely rapid accumulation was also demonstrated in the rice apoplast 12 h after infection with Magnaporthe oryzea (Shenton et al., 2012; Kim et al., 2013). Time-course profiling at the transcript level confirmed that their inductions were stronger and earlier in incompatible interactions.

Fine study of the apoplastic proteome during the early phase of the pathogen infection may also lead to the identification of proteins specifically produced by the pathogen and involved in the perception. For instance, a flagellin B homolog from Agrobacterium tumefaciens was shown to accumulate in the apoplast of tobacco agro-infiltrated leaves (Goulet et al., 2010) and the putative virulence factor cyclophilin CYP1 from Magnaporthe was found in the rice apoplast (Shenton et al., 2012). Similarly, De-La-Pena et al. (2008) showed evidence that bacteria are also able to change the proteins they secrete, depending on the identity of the plant partner. The elongation factor Tu was only secreted in the interaction between Pseudomonas syringae DC3000 and alfalfa but not with Arabidopsis, suggesting that stimulation of innate immunity by this bacterial protein could be plant-microbe dependent (De-La-Pena et al., 2008).

Kim et al. (2013) recently developed an interesting screening approach to identify putative apoplastic effectors secreted by Magnaporthe oryzae in rice leaves showing that some proteins activate the PBZ1 cell death related promoter only when they are expressed in the apoplast, acting putatively as apoplastic effectors. Therefore, apoplastic proteomic approaches may prove to be an interesting tool to discover or unravel key players involved in pathogen perception and signal transduction.

ROS and Redox Regulation

Secretome or apoplastic proteome studies under biotic stress conditions generally reveal changes of proteins involved in ROS metabolism, representing 10–30% of the secreted proteome (Tables 1, 2). Members of most of these protein families, such as peroxidases, oxalate oxidases or superoxide dismutases, are generally present in the unstressed leaf apoplast (Delaunois et al., 2013). Unsurprisingly, the H2O2 treatment of rice seedlings triggers protein changes in the apoplast, 30% of which are involved in redox homeostasis. These proteins are involved in adjusting redox plant cell status, either triggering defense reactions or overcoming the deleterious effects of oxidative stress (Figure 1). It is noteworthy that a number of redox-associated enzymes such as some peroxidases are repressed at protein levels, which might modulate the H2O2 concentration to an appropriate level. At the same time, peroxidase accumulation in apoplast has been observed during the Brassica napus/Verticillium longisporum interaction (Floerl et al., 2008), in the secretome of grapevine cell suspension treated with MeJA (Martinez-Esteso et al., 2009) or in the secretome of Arabidopsis cell suspension treated with SA (Cheng et al., 2009), revealing that either the pathogen or a related molecule signal is able to modulate the level of peroxidases in the apoplast. Moreover, the peroxidase accumulation was correlated with their transcript accumulation in the Populus/Melampsora interaction (Pechanova et al., 2010) and the Arabidopsis/Verticillium interaction (Floerl et al., 2012). However, the time-course and the degree of transcript accumulation could be different from the corresponding protein levels. Finally, in maize secretome, some peroxidases do not change in quantity but are dephosphorylated after Fusarium-elicitor treatment (Chivasa et al., 2005) suggesting a precise regulation of peroxidases in the apoplast itself through PTM. Plants respond to bacterial challenge through quantitative and qualitative changes in peroxidase secretion leading to symbiotic or defense responses. During the rice-Magnaporthe oryzae interaction, Kim et al. (2013) have observed the modulation of 20 different peroxidases suggesting an intracellular ROS homeostasis to maintain a delicate equilibrium. Similarly, the root exudate proteome analyzed during the interaction between alfalfa and the bacterial symbiont Sinorhizobium meliloti or between Arabidopsis plants and an opportunistic bacterial pathogen Pseudomonas syringae revealed a complex and fine-tuned regulation of peroxidase amount depending on the plant-bacterium combinations (De-La-Pena et al., 2008). The existence of large multigenic families of peroxidases in plants (with 138 members in rice and 73 members in Arabidopsis) with a high number of enzymatic isoforms warrants their complex and fine-tuned regulation. Peroxidases were especially involved in a broad range of plant defense mechanisms such as lignin and suberin formation, cross-linking of cell wall components, phytoalexin synthesis, and the metabolism of ROS and RNS (Almagro et al., 2009). It should be noted that during most biotic stress responses, the major sources of ROS seem to be due to cell wall localized peroxidases that generate hydrogen peroxide, or plasma membrane-localized NADPH/NADH oxidases that generate superoxide, or both systems operating in tandem (Bolwell, 1999; Daudi et al., 2012). Since the NADPH oxidases are plasma membrane localized (Lherminier et al., 2009), unsurprisingly, they are not found in the apoplast of pathogen infected plants, whereas the amount of numerous peroxidases is finely modulated. The absence of these proteins in the apoplastic fluid could even be used as a marker of the plasma membrane integrity as well as H+-ATPases.

Among all the proteins identified in the apoplastic proteome and involved in the regulation of ROS, we can also mention two oxalate oxidases (or germins) that highly accumulate in rice suspension-cultured cells treated with a rice blast fungus elicitor (Kim et al., 2009). The oxalate oxidases are involved in responses to biotic or abiotic stresses by producing H2O2 from oxalic acid. Since certain fungal pathogens produce oxalic acid, the oxalate oxidases present in apoplast could degrade the oxalic acid produced upon fungal infection to generate H2O2, which in turn may functions as a signal for plant defenses (Figure 1).

The antioxidant enzymes superoxide dismutases are also involved in ROS signaling and significantly accumulate in the secretome of Arabidopsis cell suspensions treated with SA (Cheng et al., 2009) or inoculated with Pseudomonas syringae (Kaffarnik et al., 2009). They also accumulated in the apoplastic poplar proteome infected by Melampsora medusae (Pechanova et al., 2010). Superoxide dismutase produce H2O2 from superoxides (O·−2), which is further degraded to H2O by ascorbate peroxidase. The superoxide dismutase could favor the transient nature of the oxidative burst and prevent accumulation of toxic superoxides, limiting the duration of the oxidative burst to an early event in plant defense (Scheler et al., 2013). Similarly, Sinorhizobium meliloti secretes higher amounts of superoxide dismutase in alfalfa than Arabidopsis roots, suggesting that the bacteria specifically recognize alfalfa to initiate the symbiosis (De-La-Pena et al., 2008).

Overall, these proteomic approaches reveal a strong implication of the apoplastic proteins involved in ROS homeostasis. They also highlight the fine regulation of these proteins requiring the control of their secretion as well as their activation through post-translational modifications depending on the plant-pathogen interaction.

Cell Wall Modification

As mentioned before, the cell wall is one of the most important barriers to counter pathogen invasion and it is not surprising to find in the unstressed apoplast numerous enzymes involved in cell wall modification or maintenance (Albenne et al., 2013; Komatsu and Yanagawa, 2013). It has already been described how the cell wall is actively remodeled and reinforced during infection specifically at discrete sites of interaction with pathogens (Figure 1) (Hamann, 2012; Underwood, 2012). However, a global view of the regulation of the enzymes specifically involved in the cell wall remodeling following a pathogen invasion is still incomplete. Apoplastic proteomic approaches definitely help to decipher the regulation of this complex metabolism involving numerous enzymes. Indeed, in most of the studies listed in Tables 1, 2, biotic stresses modulate the accumulation of secreted proteins involved in cell wall modification or maintenance like polygalacturonases, α-galactosidases, α-glucosidases, xylanases, xyloglucanases, and β-1,3-endoglucanases. Peroxidases (see above) also participate in cell wall reinforcement by modifying carbohydrate and structural protein polymer networks (Albenne et al., 2013) or through lignification or suberisation (Ndimba et al., 2003). Enzymes that breach the plant cell wall have also been shown to be important for fungal pathogens that lack specialized penetration structures and for necrotrophic pathogens. For example, the polygalacturonases are among the most extensively studied enzymes. They cleave the linkages between α-1,4 D-galacturonic acid residues, which are the major component of pectin, to produce non-methylated homogalacturonan. Polygalacturonases cause cell separation, tissue maceration, and release of mono- di- and three- saccharides used as nutrients by the pathogen (De Lorenzo et al., 2001). Some of these released fragments, such as oligogalacturonides, are typical DAMPs that elicit defense responses in many plants (Ridley et al., 2001; Sanabria et al., 2008; Ferrari et al., 2013) (Figure 1).

In the rice seedling apoplast, 45% of the proteins affected by H2O2 are involved in the carbohydrate and cell wall metabolism (Zhou et al., 2011). In this study, the abundance of most of the glycosylhydrolases such as α-galactosidases and β-1,3-glucanases, is found to be down-regulated. The authors suggested that the suppression of these polysaccharide hydrolases under H2O2 stress might reduce the hydrolysis of glucan and other polysaccharides altering the dynamic of remodeling of the polysaccharides to withstand the deleterious effects of oxidative stress. In comparison, the activities of α-L-arabinofuranosidases, UDP-glucose pyrophosphorylases and pectinesterases were up-regulated under H2O2 treatment suggesting that these enzymes might strengthen the cell wall by modulating polysaccharide degradation and synthesis, and increasing pectin demethylesterification. SA-treatment of Arabidopsis cell suspensions induces the accumulation of a large number of the proteins involved in general metabolism (34%) within the 6 first hours. Among them, α-galactosidase, α-1,4-glucan-protein synthase, pectinesterase, or β-fructofuranosidase are putatively involved in cell wall remodeling (Cheng et al., 2009). The fact that half of the identified secreted proteins are LSPs could partially explain the rapid extracellular accumulation of these proteins, which allows a rapid cell wall remodeling in response to defense signaling.

The agroinfiltration of tobacco leaves leads to the accumulation of a number of cell wall-modifying enzymes including galactosidases, α-L-arabinofuranosidases, β-D-xylosidases, peroxidases and proteases accounting for around 15% of the apoplastic proteins. These proteins are likely to be accumulated for cell wall maintenance and to complement constitutive defenses against bacterial pathogens (Goulet et al., 2010). Verticillium longisporum infection of Arabidopsis results in a specific increase of six extracellular proteins with overlapping functions in defense, development and cell wall metabolism (three peroxidases, germin, serine carboxypeptidase, α-galactosidase) (Floerl et al., 2012). The authors have correlated these changes in infected plants with a new synthesis of cell wall material with enhanced lignification and with a modification of metabolite contents.

Comparison of the apoplastic proteomes of Arabidopsis infected with virulent, avirulent or non-pathogenic strains of Pseudomonas syringae pv tomato revealed a strain-specific regulation of cell wall-modifying enzymes. For example, α-xylosidase accumulation is increased by MAMPs from the non-pathogenic mutant strain HrpA. By contrast, amount of the enzyme is decreased by an effector of the virulent strain DC3000, suggesting that this α-xylosidase could be important for Pseudomonas syringae resistance (Kaffarnik et al., 2009). In the same way, quantity of two glycosylhydrolases is specifically increased during the compatible Arabidopsis/Pseudomonas syringae interaction but not in the incompatible interaction with Sinorhizobium meliloti, demonstrating a microorganism-dependent regulation of these cell wall enzymes (De-La-Pena et al., 2008).

Out of the 700 proteins identified in the rice apoplast infected by Magnaporthe oryzae, 29 proteins from rice and 54 proteins from Magnaporthe oryzae were glycosylhydrolases (Kim et al., 2013). Moreover, 17 rice glycosylhydrolase genes were strongly activated at the transcriptional level after infection. Among these 17 genes, 4 glycosylhydrolases were expressed earlier or at a higher level in the incompatible interactions compared to the compatible ones. From the pathogen side, RT-PCR analysis revealed that the transcripts of 6 glycosylhydrolases families from Magnaporthe oryzae were differentially expressed in compatible interactions. Therefore, this study demonstrated that the extracellular modulation of both the pathogen and the host glycosylhydrolases have an important role in either promoting successful infection via the degradation of the host cell wall, or restricting the pathogen invasion through the reinforcement of the host defenses via the cell wall maintenance during early stages of infection.

PR and Other Defense-Related Proteins

The defense-related proteins have various functions and are generally involved in different metabolisms other than defense. It is therefore relatively difficult to classify these proteins only in terms of their defense function. Some of these proteins such as peroxidases or oxalate oxidases have already been mentioned above. The defense-related proteins represent a large part of the basal apoplastic proteome including pathogenesis-related (PR) proteins, which are the most abundant (Delaunois et al., 2013). Unsurprisingly, the amount of the PR proteins is modulated in response to biotic stress in nearly all the secretome or apoplastic proteome studies listed in Tables 1, 2. However, despite the importance of PR proteins in plant defense, they generally only represent 10–15% of the proteins that are regulated in the apoplast following biotic stresses (Cheng et al., 2009; Kaffarnik et al., 2009; Zhou et al., 2011). The well-characterized chitinases degrade the cell walls of pathogen releasing PAMP-derived cell wall fragments that trigger MTI, thereby reinforcing the host defenses (Figure 1). Indeed, Kim et al. (2009) have identified up to nine chitinases induced by the rice blast fungus in the rice secretome. While chitinases are largely represented in the apoplast, not all of them are regulated in response to defense signaling. The analysis of the apoplastic proteomes of Arabidopsis infected with virulent or non-virulent strains of Pseudomonas syringae revealed that the effector of the virulent strain repressed two chitinases but only one is induced by MAMPs from the non-pathogenic strain (Kaffarnik et al., 2009). Similarly, two chitinases were identified in the Arabidopsis secretome in response to SA but only one was accumulated within the 2 first hours after treatment (Cheng et al., 2009). In response to H2O2, the rice apoplastic proteome analysis revealed the up-regulation of one chitinase and the down-regulation of two others (Zhou et al., 2011). All together, these results clearly indicate a pathogen- or signal-specific regulation of the chitinase pool in the apoplast.

Glucanases represent another large apoplastic protein family often co-induced with chitinases (Figure 1). Glucanases are known to limit fungal growth via the degradation of the glucans from fungal cell walls. Verticillum longisporum infection of Brassica napus induces the accumulation of one endochitinase and two β-1,3-glucanases (Floerl et al., 2008). Agrobacterium infiltration of tobacco leaves modulates the quantity of several chitinases and glucanases (Goulet et al., 2010). Moreover, the changes in the phosphorylation status of an endochitinase and an endo-1,4-β-glucanase revealed by the chitosan treatment of Arabidopsis cells (Ndimba et al., 2003) and the correlation of the transcript accumulation with the increase of acidic chitinases and β-1,3-glucanases in Melampsora larici/Populus deltoides interaction suggest a close regulation of these PR protein families (Pechanova et al., 2010). It should also be mentioned that glucanases could be involved in host cell wall remodeling putatively leading to the release of DAMPs molecules and thereby reinforcing the host defenses (Casasoli et al., 2008; Martinez-Esteso et al., 2009). Besides direct modifications that glucanases can produce in the cell wall, Finiti et al. (2013) suggested that they might interfere in the signaling network that operates during the defense response. Their enzymatic products, the β-1,3 glucans, can be considered as DAMPs and are known to be general elicitors of plant defense responses. The β-1,3 glucans were shown to induce variety of defense reactions in tobacco (Klarzynski et al., 2000), Arabidopsis (Ménard et al., 2004), or grapevine (Aziz et al., 2003, 2007), conferring resistance to viral, bacterial, and fungal pathogens. Moreover previous studies have demonstrated that the absence of the endoglucanases TomCel1 and TomCel2 in tomato and Arabidopsis alters the jasmonic acid signaling network limiting the necrotrophic pathogen Botrytis cinerea invasion and increasing the susceptibility to the hemibiotrophic Pseudomonas syringae DC3000 (Flors et al., 2007; Finiti et al., 2013). These results provide support for the contribution of endoglucanases in the establishment of the appropriate signaling response to pathogens by modifying the properties of the cell wall and/or interfering with signaling pathways.

Other PR proteins are regulated in the apoplast following biotic stress. The PR1 protein is accumulated in the apoplast of agroinfiltrated tobacco or H2O2-treated rice and the thaumatin-like protein accumulates in Melampsora larici infected poplar (Goulet et al., 2010; Zhou et al., 2011). Some lipases were also thought to act like PR proteins (Jakab et al., 2003). A lipase with a GDSL-like motif was identified in the grapevine secretome in response to JA (Martinez-Esteso et al., 2009) and in the Arabidopsis secretome in response to SA (Oh et al., 2005). The Arabidopsis lipase (GLIP1) was further characterized for its function in disease resistance and results suggest that GLIP1 may be a critical component in plant resistance. Indeed, the GLIP1 lipase disrupts the fungal spore integrity and triggers systemic resistance signaling in Alternaria brassicicola infected plants through the ET pathway (Oh et al., 2005).

Proteolytic enzymes in plants are directly or indirectly involved in most plant cellular processes including disease resistance (Xia et al., 2004). The induction of the amount of three subtilisin-like proteases, two aspartyl proteases, and one peptidase in the rice apoplast during Magnaporthe oryzae infection supports the view that secreted proteolytic enzymes might act as hydrolytic enzymes or mediators of signal transduction in the apoplast during pathogen attack (Kim et al., 2013). In this study, a total of 25 proteases/peptidase proteins from Magnaporthe oryzae were identified. These proteins are believed to play roles as pathogenicity factors required to circumvent the host defense responses. Therefore, the study of protease secretion in the apoplast is a promising resource for understanding some facets of plant-pathogen interactions.

Lectins are characterized by the presence of at least one jacalin-like domain that reversibly binds specific mono- or oligosaccharides. According to their carbohydrate specificities, plant lectins are important for a variety of biological processes including host–pathogen interactions. Specifically, they are believed to play a role in pathogen recognition and in plant defense responses (De Hoff et al., 2009). Several studies have shown their accumulation in the apoplast in response to SA treatment, chitosan or oligogalacturonides in Arabidopsis (Ndimba et al., 2003; Casasoli et al., 2008; Cheng et al., 2009) or in the rice-Magnaporthe interaction (Kim et al., 2009). Their regular identification in the apoplast under biotic-stress conditions reinforces their putative role in plant defense mechanisms.

In the rice-Magnaporthe interaction, the accumulation of several PR proteins is induced 72 h after infection in both the compatible and incompatible interactions but with a higher level in the incompatible interaction (Shenton et al., 2012). In the same plant-pathogen interaction, Kim et al. (2013) correlated the PR protein accumulation with upregulation of gene expression in both types of interactions and showed that three chitinase genes were expressed earlier or at a higher level in the incompatible interactions. Six proteins related to defense, such as peroxidases and basic chitinases were highly secreted in Arabidopsis 6 h after initial contact with Pseudomonas syringae but not in the incompatible interaction with Sinorhizobium meliloti, suggesting that Arabidopsis can selectively secrete defense proteins at an early stage of compatible interactions (De-La-Pena et al., 2008). Moreover, in the interaction between alfalfa with Sinorhizobium meliloti or Pseudomonas syringae, three chitinases, a thaumatin-like protein PR-5b, and a PR10-1 protein were secreted in abundance by alfalfa inoculated with Sinorhizobium meliloti at 6 h but were not secreted as much when it was inoculated with Pseudomonas syringae. The fact that alfalfa responds faster by secreting proteins in the presence of Sinorhizobium meliloti, but not in the presence of Pseudomonas syringae, suggests that an efficient signaling process similar to that operating during pathogenic interactions takes place during the early interaction with Sinorhizobium meliloti.

Conclusions and Future Perspectives

Interest in the plant defense responses occurring in the apoplast is growing as the importance of this dynamic compartment becomes more apparent. The small number of studies indicates the limited availability of information on the potential role of the apoplastic proteome in plant-pathogen interactions. The first secretome analyses were reported using isolated MAMPs or signal molecule onto cell suspension cultures and, to our knowledge, only two secretome studies have been performed using intact pathogens (Table 1). A shift from cell-suspensions to in planta systems has taken place, but comparative disease-resistance studies are still scarce and little is known about the changes in the secretome during biotic stresses (Table 2). Based on the results of the proteomic studies reviewed here, our current understanding of biological processes occurring in the apoplast during plant–pathogen interactions is still rudimentary. Most of the proteins present in the apoplast are involved in the establishment of a basal defense in unstressed plants. Only a small number of these proteins are specifically modulated following the perception of a biotic stress.

Most of the studies listed in this review highlighted the regulation of the same families of proteins occurring in the apoplast during a biotic stress. The regulated proteins potentially involved in the mechanisms of perception and signal transduction such as DUF26 or LRR-like proteins appear to be less identified and/or characterized. The regulation of peroxidases, glucanases and chitinases is also emphasized since these large families of proteins are involved in the regulation of the cell redox status, the cell wall reorganization and the establishment of specific defenses (Figure 1). The general consensus suggests that accurate control of the speed and intensity of the protein secretion determines the establishment of effective resistance against a given pathogen.

The LSPs identified in most of the apoplastic proteomes in response to pathogen attacks may be one of the solutions used to increase the speed of protein secretion. Indeed, this secretory mechanism, independent of the classical ER-Golgi secretory pathway, could allow the rapid and efficient secretion of specific proteins providing a selective advantage in response to pathogen infection (Rose and Lee, 2010). Since most of the non-classically secreted proteins have established intracellular functions, it was suggested that they had dual roles with still unknown extracellular functions. There is growing evidence about the role of LSPs in plant defenses and the precise identification and characterization of these secreted proteins remain an exciting and challenging area of research.

Another way to increase the speed and specificity of the defense response in the apoplast may be the modulation of the PTMs of a pool of pre-proteins already present or the existence of several alternative PTMs affecting the final destination of the protein. Most of the apoplastic proteome studies in response to pathogen attacks suggested modulation of PTMs. Modification of PTMs could rapidly activate or repress the specific proteins involved in pathogen perception (like glycosylation) or signal transduction (like phosphorylation). It was demonstrated that the phosphorylation status of extracellular proteins rapidly changes in response to elicitor treatment, suggesting a possible role for the apoplastic proteins in early signal transmission of pathogen defenses through the activation of pathways regulated by external kinases and phosphatases (Casasoli et al., 2008). The establishment of plant phosphoproteomes has made remarkable progress and is now moving from qualitative to quantitative. However, more work needs to be done to investigate the precise phosphorylation nature and phosphorylation patterns. Moreover, there has been no global study of the glycosylation of apoplastic proteins upon pathogen infection. Plant glycoproteomics is only in its early stages but is a very promising approach toward an integrated study of both sugars and protein moities to gain new insight into the function of glycoproteins in plant defenses. Similarly, little is known about the oxidation of apoplastic proteins even if ROS and NO are important molecules playing key roles in apoplastic plant defenses. The study of PTMs is still in its initial phases, and although instrumentation and separation techniques can be improved, for many PTMs there are some existing methods available that can be adapted to plant disease proteomics research. Undoubtedly, future work needs to be directed toward a better understanding of the possible extracellular PTM events since the ability to define the dynamic proteome is crucial for unraveling novel mechanisms of plant-pathogen signaling.

It was suggested that secreted proteins might be a critical component in the process of signaling and recognition occurring between compatible and incompatible interactions. Infection of rice or Arabidopsis with an incompatible pathogen leads to a much earlier induction of genes and proteins than for a compatible interaction (Kaffarnik et al., 2009; Kim et al., 2013). These results highlight the importance of the early stages in the infection process and demonstrate the need for kinetic studies addressing complex organism interactions. More in-depth analyses of the spatial and temporal distribution of responding proteins will improve the understanding of pathogen invasion strategies and the complex interplay between hosts and pathogens. Future studies should focus on differential approaches based on compatible/incompatible interactions by using virulent/avirulent pathogen strains or sensitive/resistant host species. However, these studies should also include kinetics of apoplastic proteome and cell wall proteome at the early steps of the infection process to obtain an dynamic view of identified soluble and ionically bonded proteins from both the plant and the pathogen. Since a down-regulation of a protein upon pathogen attack might indicate regulation by pathogen effectors, functional analysis of a subset of identified secretory proteins from the pathogen implies that a number of them are likely to act as apoplastic effectors that can be recognized by receptors (Kim et al., 2013). As more and more evidence points to the biological role of the fungal effectors that manipulate plant immunity in favor of fungal virulence, the development of reliable quantitative proteomics will indeed be crucial to identifying putative effector targeting in the apoplastic proteome.

Thus, it is important to build a comprehensive inventory of the experimentally identified plant–pathogen secretome to predict secreted proteins more accurately, and then to address the question of their biological role. Apoplastic proteome analyses of plant–pathogen interactions have provided a better understanding of plant defense responses. However, the lack of published studies using quantitative and in vivo proteomic techniques is still striking. The improvement of peptide resolution sensitivity based on gel-free technology and the precise and absolute peptide quantification based on isotopic labeling approaches, such as iTRAQ technology, should greatly increase the number of identified apoplastic proteins upon pathogen challenge. The utility of absolute quantification of individual secreted proteins was clearly demonstrated in application to complex, time- and dose dependent experimental designs. There is also a need for performing more biological conditions rather than just technical replicates in experiments for quantification. Moreover, combining proteomic analyses with genetics and other omic approaches would strengthen the biological significance of many studies. A more systematic integration of these complementary approaches will provide useful information that will allow for better prediction and manipulation of plant responses to pathogens. Nevertheless, one of the main challenges in the near future will be to validate and explore the roles of individual secreted proteins involved in plant-pathogen interactions. While most proteomic studies provide protein identification and functional predictions, most of them do not test their hypotheses using genetics. Further studies will then be needed to assign functional roles to these secreted proteins in plant-pathogen interactions.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The authors are grateful to Grace Stockton for editorial assistance. This work was supported by the “Region Champagne Ardennes” through the project VINEAL2 and by “Comité Champagne” (Epernay, France).

References

Afroz, A., Khan, M. R., Ahsan, N., and Komatsu, S. (2009). Comparative proteomic analysis of bacterial wilt susceptible and resistant tomato cultivars. Peptides 30, 1600–1607. doi: 10.1016/j.peptides.2009.06.005

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Afroz, A., Zahur, M., Zeeshan, N., and Komatsu, S. (2013). Plant-bacterium interactions analyzed by proteomics. Front. Plant Sci. 4:21. doi: 10.3389/fpls.2013.00021

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Agrawal, G. K., Jwa, N.-S., Lebrun, M.-H., Job, D., and Rakwal, R. (2010). Plant secretome: unlocking secrets of the secreted proteins. Proteomics 10, 799–827. doi: 10.1002/pmic.200900514

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Albenne, C., Canut, H., and Jamet, E. (2013). Plant cell wall proteomics: the leadership of Arabidopsis thaliana. Front. Plant Sci. 4:111. doi: 10.3389/fpls.2013.00111

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Alexandersson, E., Ali, A., Resjö, S., and Andreasson, E. (2013). Plant secretome proteomics. Front. Plant Sci. 4:9. doi: 10.3389/fpls.2013.00009

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Almagro, L., Gómez Ros, L. V., Belchi-Navarro, S., Bru, R., Ros Barceló, A., and Pedrenõ, M. A. (2009). Class III peroxidases in plant defence reactions. J. Exp. Bot. 60, 377–390. doi: 10.1093/jxb/ern277

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Aziz, A., Gauthier, A., Bézier, A., Poinssot, B., Joubert, J.-M., Pugin, A., et al. (2007). Elicitor and resistance-inducing activities of β-1,4 cellodextrins in grapevine, comparison with β-1,3 glucans and α-1,4 oligogalacturonides. J. Exp. Bot. 58, 1463–1472. doi: 10.1093/jxb/erm008

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Aziz, A., Poinssot, B., Daire, X., Adrian, M., Bezier, A., Lambert, B., et al. (2003). Laminarin elicits defense responses in grapevine and induces protection against Botrytis cinerea and Plasmopara viticola. Mol. Plant Microbe Interact. 16, 1118–1128. doi: 10.1094/MPMI.2003.16.12.1118

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bayer, E. M., Bottrill, A. R., Walshaw, J., Vigouroux, M., Naldrett, M. J., Thomas, C. L., et al. (2006). Arabidopsis cell wall proteome defined using multidimensional protein identification technology. Proteomics 6, 301–311. doi: 10.1002/pmic.200500046

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bendtsen, J. D., Jensen, L. J., Blom, N., Von Heijne, G., and Brunak, S. (2004). Feature-based prediction of non-classical and leaderless protein secretion. Protein Eng. Des. Sel. 17, 349–356. doi: 10.1093/protein/gzh037

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bhadauria, V., Banniza, S., Wang, L.-X., Wei, Y.-D., and Peng, Y.-L. (2010). Proteomic studies of phytopathogenic fungi, oomycetes and their interactions with hosts. Eur. J. Plant Pathol. 126, 81–95. doi: 10.1007/s10658-009-9521-4

CrossRef Full Text

Bindschedler, L. V., and Cramer, R. (2011). Quantitative plant proteomics. Proteomics 11, 756–775. doi: 10.1002/pmic.201000426

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Boller, T., and Felix, G. (2009). A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu. Rev. Plant Biol. 60, 379–406. doi: 10.1146/annurev.arplant.57.032905.105346

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bolwell, G. P. (1999). Role of active oxygen species and NO in plant defence responses. Curr. Opin. Plant Biol. 2, 287–294. doi: 10.1016/S1369-5266(99)80051-X

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Borderies, G., Jamet, E., Lafitte, C., Rossignol, M., Jauneau, A., Boudart, G., et al. (2003). Proteomics of loosely bound cell wall proteins of Arabidopsis thaliana cell suspension cultures: a critical analysis. Electrophoresis 24, 3421–3432. doi: 10.1002/elps.200305608

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bostock, R. M., Kuc, J. A., and Laine, R. A. (1981). Eicosapentaenoic and arachidonic acids from Phytophthora infestans elicit fungitoxic sesquiterpenes in the potato. Science 212, 67–69. doi: 10.1126/science.212.4490.67

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Brown, N. A., Antoniw, J., and Hammond-Kosack, K. E. (2012). The predicted secretome of the plant pathogenic fungus Fusarium graminearum: a refined comparative analysis. PLoS ONE 7:e33731. doi: 10.1371/journal.pone.0033731

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Brunet, S., Thibault, P., Gagnon, E., Kearney, P., Bergeron, J. J., and Desjardins, M. (2003). Organelle proteomics: looking at less to see more. Trends Cell Biol. 13, 629–638.

Pubmed Abstract | Pubmed Full Text

Brutus, A., Sicilia, F., Macone, A., Cervone, F., and De Lorenzo, G. (2010). A domain swap approach reveals a role of the plant wall-associated kinase 1 (WAK1) as a receptor of oligogalacturonides. Proc. Natl. Acad. Sci. U.S.A. 107, 9452–9457. doi: 10.1073/pnas.1000675107

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Campostrini, N., Areces, L. B., Rappsilber, J., Pietrogrande, M. C., Dondi, F., Pastorino, F., et al. (2005). Spot overlapping in two-dimensional maps: a serious problem ignored for much too long. Proteomics 5, 2385–2395. doi: 10.1002/pmic.200401253

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Carpita, N. C., and Gibeaut, D. M. (1993). Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J. 3, 1–30. doi: 10.1111/j.1365-313X.1993.tb00007.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Casado-Vela, J., SelleìS, S., and Martínez, R. B. (2006). Proteomic analysis of tobacco mosaic virus-infected tomato (Lycopersicon esculentum M.) fruits and detection of viral coat protein. Proteomics 6, 196–206. doi: 10.1002/pmic.200500317

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Casasoli, M., Spadoni, S., Lilley, K. S., Cervone, F., De Lorenzo, G., and Mattei, B. (2008). Identification by 2-D DIGE of apoplastic proteins regulated by oligogalacturonides in Arabidopsis thaliana. Proteomics 8, 1042–1054. doi: 10.1002/pmic.200700523

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Català, C., Howe, K. J., Hucko, S., Rose, J. K. C., and Thannhauser, T. W. (2011). Towards characterization of the glycoproteome of tomato (Solanum lycopersicum) fruit using Concanavalin A lectin affinity chromatography and LC-MALDI-MS/MS analysis. Proteomics 11, 1530–1544. doi: 10.1002/pmic.201000424

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Cheng, F.-Y., Blackburn, K., Lin, Y.-M., Goshe, M. B., and Williamson, J. D. (2009). Absolute protein quantification by LC/MSE for global analysis of salicylic acid-induced plant protein secretion responses. J. Proteome Res. 8, 82–93. doi: 10.1021/pr800649s

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Chivasa, S., Hamilton, J. M., Pringle, R. S., Ndimba, B. K., Simon, W. J., Lindsey, K., et al. (2006). Proteomic analysis of differentially expressed proteins in fungal elicitor-treated Arabidopsis cell cultures. J. Exp. Bot. 57, 1553–1562. doi: 10.1093/jxb/erj149

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Chivasa, S., Simon, W., Yu, X. L., Yalpani, N., and Slabas, A. (2005). Pathogen elicitor-induced changes in the maize extracellular matrix proteome. Proteomics 5, 4894–4904. doi: 10.1002/pmic.200500047

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Cho, W. K., Chen, X. Y., Chu, H., Rim, Y., Kim, S., Kim, S. T., et al. (2009). Proteomic analysis of the secretome of rice calli. Physiol. Plant. 135, 331–341. doi: 10.1111/j.1399-3054.2008.01198.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Corpas, F. J., Del Río, L. A., and Barroso, J. B. (2008). Post-translational modifications mediated by reactive nitrogen species. Plant Signal. Behav. 3, 301–303. doi: 10.4161/psb.3.5.5277

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Cui, S., Guo, X., Chang, F., Cui, Y., Ma, L., Sun, Y., et al. (2005). Apoplastic calmodulin receptor-like binding proteins in suspension-cultured cells of Arabidopsis thaliana. J. Biol. Chem. 280, 31420–31427. doi: 10.1074/jbc.M501349200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Daudi, A., Cheng, Z., O'Brien, J., Mammarella, N., Khan, S., Ausubel, F., et al. (2012). The apoplastic oxidative burst peroxidase in Arabidopsis is a major component of pattern-triggered immunity. Plant Cell 24, 275–287. doi: 10.1105/tpc.111.093039

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

De Hoff, P. L., Brill, L. M., and Hirsch, A. M. (2009). Plant lectins: the ties that bind in root symbiosis and plant defense. Mol. Genet. Genom. 282, 1–15. doi: 10.1007/s00438-009-0460-8

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

De Lorenzo, G., D'Ovidio, R., and Cervone, F. (2001). The role of polygacturonase-inhibiting proteins (PGIPs) in defense against pathogenic fungi. Annu. Rev. Phytopathol. 39, 313–335. doi: 10.1146/annurev.phyto.39.1.313

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

De-La-Pena, C., Lei, Z., Watson, B. S., Sumner, L. W., and Vivanco, J. M. (2008). Root-microbe communication through protein secretion. J. Biol. Chem. 283, 25247–25255. doi: 10.1074/jbc.M801967200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Delannoy, M., Alves, G., Vertommen, D., Ma, J., Boutry, M., and Navarre, C. (2008). Identification of peptidases in Nicotiana tabacum leaf intercellular fluid. Proteomics 8, 2285–2298. doi: 10.1002/pmic.200700507

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Delaunois, B., Colby, T., Belloy, N., Conreux, A., Harzen, A., Baillieul, F., et al. (2013). Large-scale proteomic analysis of the grapevine leaf apoplastic fluid reveals mainly stress-related proteins and cell wall modifying enzymes. BMC Plant Biol. 13:24. doi: 10.1186/1471-2229-13-24

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Denoux, C., Galletti, R., Mammarella, N., Gopalan, S., Werck, D., De Lorenzo, G., et al. (2008). Activation of defense response pathways by OGs and Flg22 elicitors in Arabidopsis seedlings. Mol. Plant 1, 423–445. doi: 10.1093/mp/ssn019.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Di Carli, M., Benvenuto, E., and Donini, M. (2012). Recent insights into plant-virus interactions through proteomic analysis. J. Proteome Res. 11, 4765–4780. doi: 10.1021/pr300494e

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Doehlemann, G., and Hemetsberger, C. (2013). Apoplastic immunity and its suppression by filamentous plant pathogens. New Phytol. 198, 1001–1016. doi: 10.1111/nph.12277

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ellis, J. G., Dodds, P. N., and Lawrence, G. J. (2007). The role of secreted proteins in diseases of plants caused by rust, powdery mildew and smut fungi. Curr. Opin. Microbiol. 10, 326–331. doi: 10.1016/j.mib.2007.05.015

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Emanuelsson, O., Brunak, S., Von Heijne, G., and Nielsen, H. (2007). Locating proteins in the cell using TargetP, SignalP and related tools. Nat. Protocol. 2, 953–971. doi: 10.1038/nprot.2007.131

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Erbs, G., and Newman, M. A. (2012). The role of lipopolysaccharide and peptidoglycan, two glycosylated bacterial microbe-associated molecular patterns (MAMPs), in plant innate immunity. Mol. Plant Pathol. 13, 95–104. doi: 10.1111/j.1364-3703.2011.00730.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Felix, G., and Boller, T. (2003). Molecular sensing of bacteria in plants. J. Biol. Chem. 278, 6201–6208. doi: 10.1074/jbc.M209880200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Fernandez Acero, F. J., Colby, T., Harzen, A., Cantoral, J. M., and Schmidt, J. (2009). Proteomic analysis of the phytopathogenic fungus Botrytis cinerea during cellulose degradation. Proteomics 9, 2892–2902. doi: 10.1002/pmic.200800540

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Fernandez, M. B., Pagano, M. R., Daleo, G. R., and Guevara, M. G. (2012). Hydrophobic proteins secreted into the apoplast may contribute to resistance against Phytophthora infestans in potato. Plant Physiol. Biochem. 60, 59–66. doi: 10.1016/j.plaphy.2012.07.017

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ferrari, S., Savatin, D., Sicilia, F., Gramegna, G., Cervone, F., and De Lorenzo, G. (2013). Oligogalacturonides: plant damage-associated molecular patterns and regulators of growth and development. Front. Plant Sci. 4:49. doi: 10.3389/fpls.2013.00049

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Finiti, I., Leyva, M. O., López-Cruz, J., Calderan Rodrigues, B., Vicedo, B., Angulo, C., et al. (2013). Functional analysis of endo-1,4-β-glucanases in response to Botrytis cinerea and Pseudomonas syringae reveals their involvement in plant–pathogen interactions. Plant Biol. 15, 819–831. doi: 10.1111/j.1438-8677.2012.00701.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Floerl, S., Druebert, C., Majcherczyk, A., Karlovsky, P., Kües, U., and Polle, A. (2008). Defence reactions in the apoplastic proteome of oilseed rape (Brassica napus var. napus) attenuate Verticillium longisporum growth but not disease symptoms. BMC Plant Biol. 18:129. doi: 10.1186/1471-2229-8-129

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Floerl, S., Majcherczyk, A., Possienke, M., Feussner, K., Tappe, H., Gatz, C., et al. (2012). Verticillium longisporum infection affects the leaf apoplastic proteome, metabolome, and cell wall properties in Arabidopsis thaliana. PLoS ONE 7:e31435. doi: 10.1371/journal.pone.0031435

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Flors, V., Leyva, M. L., Vicedo, B., Finiti, I., Real, M. D., Garcia-Agustin, P., et al. (2007). Absence of the endo-beta-1,4-glucanases cel1 and Cel2 reduces susceptibility to Botrytis cinerea in tomato. Plant J. 52, 1027–1040. doi: 10.1111/j.1365-313X.2007.03299.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Garcia-Brugger, A., Lamotte, O., Vandelle, E., Bourque, S. P., Lecourieux, D., Poinssot, B., et al. (2006). Early signaling events induced by elicitors of plant defenses. Mol. Plant Microbe Interact. 19, 711–724. doi: 10.1094/MPMI-19-0711

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Geddes, J., Eudes, F., Laroche, A., and Selinger, L. B. (2008). Differential expression of proteins in response to the interaction between the pathogen Fusarium graminearum and its host, Hordeum vulgare. Proteomics 8, 545–554. doi: 10.1002/pmic.200700115

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Gimenez-Ibanez, S., and Solano, R. (2013). Nuclear jasmonate and salicylate signaling and crosstalk in defense against pathogens. Front. Plant Sci. 4:72. doi: 10.3389/fpls.2013.00072

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Girard, V., Dieryckx, C., Job, C., and Job, D. (2013). Secretomes: the fungal strike force. Proteomics 13, 597–608. doi: 10.1002/pmic.201200282

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Giribaldi, M., Purrotti, M., Pacifico, D., Santini, D., Mannini, F., Caciagli, P., et al. (2011). A multidisciplinary study on the effects of phloem-limited viruses on the agronomical performance and berry quality of Vitis vinifera cv. Nebbiolo. J. Proteom. 75, 306–315. doi: 10.1016/j.jprot.2011.08.006

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Glazebrook, J. (2005). Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu. Rev. Phytopathol. 43, 205–227. doi: 10.1146/annurev.phyto.43.040204.135923

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Goldberg, T., Hamp, T., and Rost, B. (2012). LocTree2 predicts localization for all domains of life. Bioinformatics 28, i458–i465. doi: 10.1093/bioinformatics/bts390

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Görg, A., Weiss, W., and Dunn, M. (2004). Current two-dimensional electrophoresis technology for proteomics. Proteomics 4, 3665–3685. doi: 10.1002/pmic.200401031

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Goulet, C., Goulet, C., Goulet, M.-C., and Michaud, D. (2010). 2-DE proteome maps for the leaf apoplast of Nicotiana benthamiana. Proteomics 10, 2536–2544. doi: 10.1002/pmic.200900382

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Grimsrud, P. A., Den Os, D., Wenger, C. D., Swaney, D. L., Schwartz, D., Sussman, M. R., et al. (2010). Large-scale phosphoprotein analysis in Medicago truncatula roots provides insight into in vivo kinase activity in legumes. Plant Physiol. 152, 19–28. doi: 10.1104/pp.109.149625

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Hamann, T. (2012). Plant cell wall integrity maintenance as an essential component of biotic stress response mechanisms. Front. Plant Sci. 3:1–5. doi: 10.3389/fpls.2012.00077

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Hernández, J. A., Ferrer, M., Jiménez, A., Barceló, A. R., and Sevilla, F. (2001). Antioxidant systems and O2·/H2O2 production in the apoplast of pea leaves. Plant Physiol. 127, 817–831. doi: 10.1104/pp.010188

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Horbach, R., Navarro-Quesada, A., Knogge, W., and Deising, H. (2011). When and how to kill a plant cell: infection strategies of plant pathogenic fungi. J. Plant Physiol. 168, 51–62. doi: 10.1016/j.jplph.2010.06.014

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jakab, G., Manrique, A., Zimmerli, L., Metraux, J.-P., and Mauch-Mani, B. (2003). Molecular characterization of a novel lipase-like pathogen-inducible gene family of Arabidopsis. Plant Physiol. 132, 2230–2239. doi: 10.1104/pp.103.025312

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jamet, E., Albenne, C., Boudart, G., Irshad, M., Canut, H., and Pont-Lezica, R. (2008). Recent advances in plant cell wall proteomics. Proteomics 8, 893–908. doi: 10.1002/pmic.200700938

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jayaraman, D., Forshey, K. L., Grimsrud, P. A., and Ane, J. M. (2012). Leveraging proteomics to understand plant-microbe interactions. Front. Plant Sci. 3:44. doi: 10.3389/fpls.2012.00044

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jeandet, P., Clément, C., Courot, E., and Cordelier, S. (2013). Modulation of phytoalexin biosynthesis in engineered plants for disease resistance. Int. J. Mol. Sci. 14, 14136–14170. doi: 10.3390/ijms140714136

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jones, A. M. E., Thomas, V., Bennett, M. H., Mansfield, J., and Grant, M. (2006). Modifications to the Arabidopsis defence proteome occur prior to significant transcriptional change in response to inoculation with Pseudomonas syringae. Plant Physiol. 142, 1603–1620. doi: 10.1104/pp.106.086231

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jones, J. D. G., and Dangl, J. L. (2006). The plant immune system. Nature 444, 323–329. doi: 10.1038/nature05286

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Jung, Y.-H., Jeong, S.-H., Kim, S. H., Singh, R., Lee, J.-E., Cho, Y.-S., et al. (2008). Systematic secretome analyses of rice leaf and seed callus suspension-cultured cells: workflow development and establishment of high-density two-dimensional gel reference maps. J. Proteome Res. 7, 5187–5210. doi: 10.1021/pr8005149

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kaffarnik, F. A., Jones, A. M., Rathjen, J. P., and Peck, S. C. (2009). Effector proteins of the bacterial pathogen Pseudomonas syringae alter the extracellular proteome of the host plant, Arabidopsis thaliana. Mol. Cell Proteomics 8, 145–156. doi: 10.1074/mcp.M800043-MCP200

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kaku, H., Nishizawa, Y., Ishii-Minami, N., Akimoto-Tomiyama, C., Dohmae, N., Takio, K., et al. (2006). Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor. Proc. Natl. Acad. Sci. U.S.A. 103, 11086–11091. doi: 10.1073/pnas.0508882103

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Karpinska, B., Karlsson, M., Schinkel, H., Streller, S., Süss, K.-H., Melzer, M., et al. (2001). A novel superoxide dismutase with a high isoelectric point in higher plants. Plant Physiol. 126, 1668–1677. doi: 10.1104/pp.126.4.1668

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kav, N. N. V., Srivastava, S., Yajima, W., and Sharma, N. (2007). Application of proteomics to investigate plant-microbe interactions. Curr. Proteomics 4, 28–43. doi: 10.2174/157016407781387357

CrossRef Full Text

Kim, S. G., Wang, Y., Lee, K. H., Park, Z.-Y., Park, J., Wu, J., et al. (2013). In-depth insight into in vivo apoplastic secretome of rice-Magnaporthe oryzae interaction. J. Proteomics 78, 58–71. doi: 10.1016/j.jprot.2012.10.029

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kim, S. T., Cho, K. S., Yu, S., Kim, S. G., Hong, J. C., Han, C.-D., et al. (2003). Proteomic analysis of differentially expressed proteins induced by rice blast fungus and elicitor in suspension-cultured rice cells. Proteomics 3, 2368–2378. doi: 10.1002/pmic.200300577

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kim, S. T., Kang, Y. H., Wang, Y., Wu, J., Park, Z. Y., Rakwal, R., et al. (2009). Secretome analysis of differentially induced proteins in rice suspension-cultured cells triggered by rice blast fungus and elicitor. Proteomics 9, 1302–1313. doi: 10.1002/pmic.200800589

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kim, S., Kim, S., Hwang, D., Kang, S., Kim, H., Lee, B., et al. (2004a). Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea. Proteomics 4, 3569–3578. doi: 10.1002/pmic.200400999

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kim, S. T., Kim, S. G., Hwang, D. H., Kang, S. Y., Kim, H. J., Lee, B. H., et al. (2004b). Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea. Proteomics 4, 3569–3578. doi: 10.1002/pmic.200400999

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Klarzynski, O., Plesse, B., Joubert, J. M., Yvin, J. C., Kopp, M., Kloareg, B., et al. (2000). Linear beta-1,3 glucans are elicitors of defense responses in tobacco. Plant Physiol. 124, 1027–1038. doi: 10.1104/pp.124.3.1027

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Komatsu, S., and Yanagawa, Y. (2013). Cell wall proteomics of crops. Front. Plant Sci. 4:17. doi: 10.3389/fpls.2013.00017

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Konozy, E. H. E., Rogniaux, H., Causse, M., and Faurobert, M. (2012). Proteomic analysis of tomato (Solanum lycopersicum) secretome. J. Plant Res. 126, 251–266. doi: 10.1007/s10265-012-0516-4

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Kusumawati, L., Imin, N., and Djordjevic, M. A. (2008). Characterization of the secretome of suspension cultures of Medicago species reveals proteins important for defense and development. J. Proteome Res. 7, 4508–4520. doi: 10.1021/pr800291z

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Laluk, K., and Mengiste, T. (2010). Necrotroph attacks on plants: wanton destruction or covert extortion? Arabidopsis Book 8, e0136. doi: 10.1199/tab.0136

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Lee, J., Lei, Z., Watson, B. S., and W., S. L. (2013). Sub-cellular proteomics of Medicago truncatula. Front. Plant Sci. 4:112. doi: 10.3389/fpls.2013.00112

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Lherminier, J., Elmayan, T., Fromentin, J., Elaraqui, K. T., Vesa, S., Morel, J., et al. (2009). NADPH oxidase-mediated reactive oxygen species production: subcellular localization and reassessment of its role in plant defense. Mol. Plant Microbe Interact. 22, 868–881. doi: 10.1094/MPMI-22-7-0868

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Li, D., Wang, L., Teng, S., Zhang, G., Guo, L., Mao, Q., et al. (2012). Proteomics analysis of rice proteins up-regulated in response to bacterial leaf streak disease. J. Plant Biol. 55, 316–324. doi: 10.1007/s12374-011-0346-2

CrossRef Full Text

Li, K., Xu, C., and Zhang, J. (2011). Proteome profile of maize (Zea Mays L.) leaf tissue at the flowering stage after long-term adjustment to rice black-streaked dwarf virus infection. Gene 485, 106–113. doi: 10.1016/j.gene.2011.06.016

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Liao, M., Li, Y., and Wang, Z. (2009). Identification of elicitor-responsive proteins in rice leaves by a proteomic approach. Proteomics 9, 2809–2819. doi: 10.1002/pmic.200800192

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Lin, A., Wang, Y., Tang, J., Xue, P., Li, C., Liu, L., et al. (2012). Nitric oxide and protein S-nitrosylation are integral to hydrogen peroxide-induced leaf cell death in rice. Plant Physiol. 158, 451–464. doi: 10.1104/pp.111.184531

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Lohaus, G., Pennewiss, K., Sattelmacher, B., Hussmann, M., and Hermann Muehling, K. (2001). Is the infiltration-centrifugation technique appropriate for the isolation of apoplastic fluid? A critical evaluation with different plant species. Physiol. Plant. 111, 457–465. doi: 10.1034/j.1399-3054.2001.1110405.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Lounifi, I., Arc, E., Molassiotis, A., Job, D., Rajjou, L., and Tanou, G. (2013). Interplay between protein carbonylation and nitrosylation in plants. Proteomics 13, 568–578. doi: 10.1002/pmic.201200304

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Macarisin, D., Wisniewski, M. E., Bassett, C., and Thannhauser, T. W. (2009). Proteomic analysis of β-aminobutyric acid priming and abscisic acid – induction of drought resistance in crabapple (Malus pumila): effect on general metabolism, the phenylpropanoid pathway and cell wall enzymes. Plant Cell Environ. 32, 1612–1631. doi: 10.1111/j.1365-3040.2009.02025.x

CrossRef Full Text

Martinez-Esteso, M. J., Sellés-Marchart, S., Vera-Urbina, J. C., Pedreño, M. A., and Bru-Martinez, R. (2009). Changes of defense proteins in the extracellular proteome of grapevine (Vitis vinifera cv. Gamay) cell cultures in response to elicitors. J. Proteomics 73, 331–341. doi: 10.1016/j.jprot.2009.10.001

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Mathesius, U. (2009). Comparative proteomic studies of root–microbe interactions. J. Proteomics 72, 353–366. doi: 10.1016/j.jprot.2008.12.006

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ménard, R., Alban, S., De Ruffray, P., Jamois, F., Franz, G., Fritig, B., et al. (2004). β-1,3 glucan sulfate, but not β-1,3 glucan, induces the salicylic acid signaling pathway in Tobacco and Arabidopsis. Plant Cell 16, 3020–3032. doi: 10.1105/tpc.104.024968

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Micali, C. O., Neumann, U., Grunewald, D., Panstruga, R., and O'Connell, R. (2011). Biogenesis of a specialized plant-fungal interface during host cell internalization of Golovinomyces orontii haustoria. Cell Microbiol. 13, 210–226. doi: 10.1111/j.1462-5822.2010.01530

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Minic, Z., Jamet, E., Négroni, L., Der Garabedian, A. P., Zivy, M., and Jouanin, L. (2007). A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin a is enriched in cell wall glycoside hydrolases. J. Exp. Bot. 58, 2503–2512. doi: 10.1093/jxb/erm082

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Miya, A., Albert, P., Shinya, T., Desaki, Y., Ichimura, K., Shirasu, K., et al. (2007). CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 104, 19613–19618. doi: 10.1073/pnas.0705147104

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Mukherjee, A., Carp, M., Zuchman, R., Ziv, T., Horwitz, B., and Gepstein, S. (2010). Proteomics of the response of Arabidopsis thaliana to infection with Alternaria brassicicola. J. Proteomics 73, 709–720. doi: 10.1016/j.jprot.2009.10.005

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ndimba, B. K., Chivasa, S., Hamilton, J. M., Simon, W. J., and Slabas, A. R. (2003). Proteomic analysis of changes in the extracellular matrix of Arabidopsis cell suspension cultures induced by fungal elicitors. Proteomics 3, 1047–1059. doi: 10.1002/pmic.200300413

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Newman, M.-A., Sundelin, T., Nielsen, J. T., and Erbs, G. (2013). MAMP (microbe-associated molecular pattern) triggered immunity in plants. Front. Plant Sci. 4:139. doi: 10.3389/fpls.2013.00139

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Newman, M.-A., Von Roepenack-Lahaye, E., Parr, A., Daniels, M. J., and Dow, J. M. (2002). Prior exposure to lipopolysaccharide potentiates expression of plant defenses in response to bacteria. Plant J. 29, 487–495. doi: 10.1046/j.0960-7412.2001.00233.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Oh, I. S., Park, A. R., Bae, M. S., Kwon, S. J., Kim, Y. S., Lee, J. E., et al. (2005). Secretome analysis reveals an Arabidopsis lipase involved in defense against Alternaria brassicicola. Plant Cell 17, 2832–2847. doi: 10.1105/tpc.105.034819

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Okushima, Y., Koizumi, N., Kusano, T., and Sano, H. (2000). Secreted proteins of tobacco cultured BY2 cells: identification of a new member of pathogenesis-related proteins. Plant Mol. Biol. 42, 479–488. doi: 10.1023/A:1006393326985

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Pan, B., Sheng, J., Sun, W., Zhao, Y., Hao, P., and Li, X. (2013). OrysPSSP: a comparative platform for small secreted proteins from rice and other plants. Nucleic Acids Res. 41, D1192–D1198. doi: 10.1093/nar/gks1090

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Pearce, G., Strydom, D., Johnson, S., and Ryan, C. A. (1991). A polypeptide from tomato leaves induces wound-inducible proteinase inhibitor proteins. Science 253, 895–897. doi: 10.1126/science.253.5022.895

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Pechanova, O., Hsu, C. Y., Adams, J. P., Pechan, T., Vandervelde, L., Drnevich, J., et al. (2010). Apoplast proteome reveals that extracellular matrix contributes to multistress response in poplar. BMC Genomics 11:674. doi: 10.1186/1471-2164-11-674

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Pel, M. J., and Pieterse, C. M. (2013). Microbial recognition and evasion of host immunity. J. Exp. Bot. 64, 1237–1248. doi: 10.1093/jxb/ers262.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Petersen, T. N., Brunak, S., Von Heijne, G., and Nielsen, H. (2011). SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat. Methods 8, 785–786. doi: 10.1038/nmeth.1701

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Phalip, V., Delalande, F., Carapito, C., Goubet, F., Hatsch, D., Leize-Wagner, E., et al. (2005). Diversity of the exoproteome of Fusarium graminearum grown on plant cell wall. Curr. Genet. 48, 366–379. doi: 10.1007/s00294-005-0040-3

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Pieterse, C. M., Van Der Does, D., Zamioudis, C., Leon-Reyes, A., and Van Wees, S. C. (2012). Hormonal modulation of plant immunity. Annu. Rev. Cell Dev. Biol. 28, 489–521. doi: 10.1146/annurev-cellbio-092910-154055

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Quirino, B. F., Candido, E. S., Campos, P. F., Franco, O. L., and Krüger, R. H. (2010). Proteomic approaches to study plant-pathogen interactions. Phytochemistry 71, 351–362. doi: 10.1016/j.phytochem.2009.11.005

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Rajjou, L., Belghazi, M., Huguet, R., Robin, C., Moreau, A., Job, C., et al. (2006). Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms. Plant Physiol. 141, 910–923. doi: 10.1104/pp.106.082057

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ridley, B. L., O'Neill, M. A., and Mohnen, D. (2001). Pectins: structure, biosynthesis, and oligogalacturonide-related signaling. Phytochemistry 57, 929–967. doi: 10.1016/S0031-9422(01)00113-3

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Robert-Seilaniantz, A., Grant, M., and Jones, J. D. (2011). Hormone crosstalk in plant disease and defense: more than just jasmonate-salicylate antagonism. Annu. Rev. Phytopathol. 49, 317–343. doi: 10.1146/annurev-phyto-073009-114447

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Roe, M. R., and Griffin, T. J. (2006). Gel-free mass spectrometry-based high throughput proteomics: tools for studying biological response of proteins and proteomes. Proteomics 6, 4678–4687. doi: 10.1002/pmic.200500876

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Romero-Puertas, M. C., Campostrini, N., Mattè, A., Righetti, P. G., Perazzolli, M., Zolla, L., et al. (2008). Proteomic analysis of S-nitrosylated proteins in Arabidopsis thaliana undergoing hypersensitive response. Proteomics 8, 1459–1469. doi: 10.1002/pmic.200700536

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Rose, J. K. C., and Lee, S.-J. (2010). Straying off the highway: trafficking of secreted plant proteins and complexity in the plant cell wall proteome. Plant Physiol. 153, 433–436. doi: 10.1104/pp.110.154872

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Sakurai, N. (1998). Dynamic function and regulation of apoplast in the plant body. J. Plant Res. 111, 133–148. doi: 10.1007/BF02507160

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Sanabria, N., Goring, D., Nurnberger, T., and Dubery, I. (2008). Self/nonself perception and recognition mechanisms in plants: a comparison of self-incompatibility and innate immunity. New Phytol. 178, 503–513. doi: 10.1111/j.1469-8137.2008.02403.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Sanchez, L., Courteaux, B., Hubert, J., Kauffmann, S., Renault, J. H., Clement, C., et al. (2012). Rhamnolipids elicit defense responses and induce disease resistance against biotrophic, hemibiotrophic, and necrotrophic pathogens that require different signaling pathways in Arabidopsis and highlight a central role for salicylic acid. Plant Physiol. 160, 1630–1641. doi: 10.1104/pp.112.201913

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Savchenko, T., Walley, J. W., Chehab, E. W., Xiao, Y., Kaspi, R., Pye, M. F., et al. (2010). Arachidonic acid: an evolutionarily conserved signaling molecule modulates plant stress signaling networks. Plant Cell 22, 3193–3205. doi: 10.1105/tpc.110.073858

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Scheler, C., Durner, J., and Astier, J. (2013). Nitric oxide and reactive oxygen species in plant biotic interactions. Curr. Opin. Plant Biol. 16, 534–539. doi: 10.1016/j.pbi.2013.06.020

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Schenk, P. M., Kazan, K., Manners, J. M., Anderson, J. P., Simpson, R. S., Wilson, I. W., et al. (2003). Systemic gene expression in Arabidopsis during an incompatible interaction with Alternaria brassicicola. Plant Physiol. 132, 999–1010. doi: 10.1104/pp.103.021683

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Schweizer, P., Felix, G., Buchala, A., Müller, C., and Métraux, J. P. (1996). Perception of free cutin monomers by plant cells. Plant J. 10, 331–341. doi: 10.1046/j.1365-313X.1996.10020331.x

CrossRef Full Text

Serna-Sanz, A., Parniske, M., and Peck, S. C. (2011). Phosphoproteome analysis of Lotus japonicus roots reveals shared and distinct components of symbiosis and defense. Mol. Plant Microbe Interact. 24, 932–937. doi: 10.1094/MPMI-09-10-0222

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Shah, P., Powell, A. L. T., Orlando, R., Bergmann, C., and Gutierrez-Sanchez, G. (2012). Proteomic analysis of ripening tomato fruit infected by Botrytis cinerea. J. Proteome Res. 11, 2178–2192. doi: 10.1021/pr200965c

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Shen, S., Jing, Y., and Kuang, T. (2003). Proteomics approach to identify wound-response related proteins from rice leaf sheath. Proteomics 3, 527–535. doi: 10.1002/pmic.200390066

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Shenton, M. R., Berberich, T., Kamo, M., Yamashita, T., Taira, H., and Terauchi, R. (2012). Use of intercellular washing fluid to investigate the secreted proteome of the rice–Magnaporthe interaction. J. Plant Res. 125, 311–316. doi: 10.1007/s10265-012-0473-y

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Soares, N. C., Francisco, R., Ricardo, C. P., and Jackson, P. A. (2007). Proteomics of ionically bound and soluble extracellular proteins in Medicago truncatula leaves. Proteomics 7, 2070–2082. doi: 10.1002/pmic.200600953

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Spoel, S. H., and Loake, G. J. (2011). Redox-based protein modifications: the missing link in plant immune signalling. Curr. Opin. Plant Biol. 14, 358–364. doi: 10.1016/j.pbi.2011.03.007

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Trdá, L., Fernandez, O., Boutrot, F., Héloir, M.-C., Kelloniemi, J., Daire, X., et al. (2013). The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria. New Phytol. 201, 1371–1384. doi: 10.1111/nph.12592

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Tseng, T.-T., Tyler, B. M., and Setubal, J. C. (2009). Protein secretion systems in bacterial-host associations, and their description in the gene ontology. BMC Microbiol. 9:S2. doi: 10.1186/1471-2180-9-S1-S2

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Tsuda, K., and Katagiri, F. (2010). Comparing signaling mechanisms engaged in pattern-triggered and effector-triggered immunity. Curr. Opin. Plant Biol. 13, 459–465. doi: 10.1016/j.pbi.2010.04.006

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Umemoto, N., Kakitani, M., Iwamatsu, A., Yoshikawa, M., Yamaoka, N., and Ishida, I. (1997). The structure and function of a soybean β-glucan-elicitor-binding-protein. Proc. Natl. Acad. Sci. U.S.A. 94, 1029–1034. doi: 10.1073/pnas.94.3.1029

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Underwood, W. (2012). The plant cell wall: a dynamic barrier against pathogen invasion. Front. Plant Sci. 3:85. doi: 10.3389/fpls.2012.00085

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Van Loon, L. C., Rep, M., and Pieterse, C. M. J. (2006). Significance of inducible defense-related proteins in infected plants. Annu. Rev. Phytopathol. 44, 135–162. doi: 10.1146/annurev.phyto.44.070505.143425

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Varnier, A. L., Sanchez, L., Vatsa, P., Boudesocque, L., Garcia-Brugger, A., Rabenoelina, F., et al. (2009). Bacterial rhamnolipids are novel MAMPs conferring resistance to Botrytis cinerea in grapevine. Plant Cell Environ. 32, 178–193. doi: 10.1111/j.1365-3040.2008.01911.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Wen, F., Vanetten, H. D., Tsaprailis, G., and Hawes, M. C. (2007). Extracellular proteins in pea root tip and border cell exudates. Plant Physiol. 143, 773–783. doi: 10.1104/pp.106.091637

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Willmann, R., Lajunen, H. M., Erbs, G., Newman, M. A., Kolb, D., Tsuda, K., et al. (2011). Arabidopsis lysin-motif proteins LYM1 LYM3 CERK1 mediate bacterial peptidoglycan sensing and immunity to bacterial infection. Proc. Natl. Acad. Sci. U.S.A. 108, 19824–19829. doi: 10.1073/pnas.1112862108

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Witzel, K., Shahzad, M., Matros, A., Mock, H. P., and Muhling, K. H. (2011). Comparative evaluation of extraction methods for apoplastic proteins from maize leaves. Plant Methods 7, 48. doi: 10.1186/1746-4811-7-48

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Xia, Y., Suzuki, H., Borevitz, J., Blount, J., Guo, Z., Patel, K., Dixon, R. A., and Lamb, C. (2004). An extracellular aspartic protease functions in Arabidopsis disease resistance signaling. EMBO Journal 23, 980–988. doi: 10.1038/sj.emboj.7600086

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Yamaguchi, Y., and Huffaker, A. (2011). Endogenous peptide elicitors in higher plants. Curr. Opin. Plant Biol. 14, 351–357. doi: 10.1016/j.pbi.2011.05.001

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Zhang, X., Dai, Y., Xiong, Y., Defraia, C., Li, J., Dong, X., et al. (2007). Overexpression of Arabidopsis MAP kinase kinase 7 leads to activation of plant basal and systemic acquired resistance. Plant J. 52, 1066–1079. doi: 10.1111/j.1365-313X.2007.03294.x

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Zhang, Y., Giboulot, A., Zivy, M., Valot, B., Jamet, E., and Albenne, C. (2011). Combining various strategies to increase the coverage of the plant cell wall glycoproteome. Phytochemistry 72, 1109–1123. doi: 10.1016/j.phytochem.2010.10.019

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Zhou, L., Bokhari, S. A., Dong, C.-J., and Liu, J.-Y. (2011). Comparative proteomics analysis of the root apoplasts of rice seedlings in response to hydrogen peroxide. PLoS ONE 6:e16723. doi: 10.1371/journal.pone.0016723

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Zipfel, C. (2009). Early molecular events in PAMP-triggered immunity. Curr. Opin. Plant Biol. 12, 414–420. doi: 10.1016/j.pbi.2009.06.003

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Keywords: apoplast, cell wall, proteomics, secretome, pathogen, defense, MAMP

Citation: Delaunois B, Jeandet P, Clément C, Baillieul F, Dorey S and Cordelier S (2014) Uncovering plant-pathogen crosstalk through apoplastic proteomic studies. Front. Plant Sci. 5:249. doi: 10.3389/fpls.2014.00249

Received: 14 February 2014; Accepted: 15 May 2014;
Published online: 03 June 2014.

Edited by:

Jean-Pierre Metraux, Université de Fribourg, Switzerland

Reviewed by:

Murray Grant, University of Exeter, UK
Richard Bostock, University of California, Davis, USA

Copyright © 2014 Delaunois, Jeandet, Clément, Baillieul, Dorey and Cordelier. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Sylvain Cordelier, Laboratoire Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne-EA 4707, Université de Reims Champagne-Ardenne, Moulin de la Housse – BP 1039, 51687 Reims cedex 2, France e-mail: sylvain.cordelier@univ-reims.fr

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