This study describes the community structure of the microbial eukaryotic community from hypersaline Lake Tyrrell, Australia, using near full length 18S rRNA sequences. Water samples were taken in both summer and winter over a 4-year period. The extent of eukaryotic diversity detected was low, with only 35 unique phylotypes using a 97% sequence similarity threshold. The water samples were dominated (91%) by a novel cluster of the Alveolate, Apicomplexa Colpodella spp., most closely related to C. edax. The Chlorophyte, Dunaliella spp. accounted for less than 35% of water column samples. However, the eukaryotic community entrained in a salt crust sample was vastly different and was dominated (83%) by the Dunaliella spp. The patterns described here represent the first observation of microbial eukaryotic dynamics in this system and provide a multiyear comparison of community composition by season. The lack of expected seasonal distribution in eukaryotic communities paired with abundant nanoflagellates suggests that grazing may significantly structure microbial eukaryotic communities in this system.
Keywords: microbial eukaryotes, diversity, hypersaline, saltern, Dunaliella, Colpodella, 18S rRNA
Citation: Heidelberg KB, Nelson WC, Holm JB, Eisenkolb N, Andrade K and Emerson JB (2013) Characterization of eukaryotic microbial diversity in hypersaline Lake Tyrrell, Australia. Front. Microbiol. 4:115. doi: 10.3389/fmicb.2013.00115
Received: 31 July 2012; Accepted: 24 April 2013;
Published online: 13 May 2013.
Edited by:Stefan Bertilsson, Uppsala University, Sweden
Reviewed by:Karen G. Lloyd, Aarhus University, Denmark
Copyright © 2013 Heidelberg, Nelson, Holm, Eisenkolb, Andrade and Emerson. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
*Correspondence: Karla B. Heidelberg, Department of Biology, University of Southern California, 3616 Trousdale Parkway, AHF 231, Los Angeles, CA 90089, USA. e-mail: email@example.com