Original Research ARTICLE

Front. Microbiol., 03 September 2013 | doi: 10.3389/fmicb.2013.00262

Constraints on superoxide mediated formation of manganese oxides

  • 1Institute for Great Lakes Research and Department of Earth and Atmospheric Sciences, Central Michigan University, Mount Pleasant, MI, USA
  • 2Department of Chemistry and Geochemistry, Colorado School of Mines, Golden, CO, USA
  • 3School of Geology and Geophysics, University of Oklahoma, Norman, OK, USA
  • 4Marine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA

Manganese (Mn) oxides are among the most reactive sorbents and oxidants within the environment, where they play a central role in the cycling of nutrients, metals, and carbon. Recent discoveries have identified superoxide (O2) both of biogenic and abiogenic origin as an effective oxidant of Mn(II) leading to the formation of Mn oxides. Here we examined the conditions under which abiotically produced superoxide led to oxidative precipitation of Mn and the solid-phases produced. Oxidized Mn, as both aqueous Mn(III) and Mn(III/IV) oxides, was only observed in the presence of active catalase, indicating that hydrogen peroxide (H2O2), a product of the reaction of O2 with Mn(II), inhibits the oxidation process presumably through the reduction of Mn(III). Citrate and pyrophosphate increased the yield of oxidized Mn but decreased the amount of Mn oxide produced via formation of Mn(III)-ligand complexes. While complexing ligands played a role in stabilizing Mn(III), they did not eliminate the inhibition of net Mn(III) formation by H2O2. The Mn oxides precipitated were highly disordered colloidal hexagonal birnessite, similar to those produced by biotically generated superoxide. Yet, in contrast to the large particulate Mn oxides formed by biogenic superoxide, abiotic Mn oxides did not ripen to larger, more crystalline phases. This suggests that the deposition of crystalline Mn oxides within the environment requires a biological, or at least organic, influence. This work provides the first direct evidence that, under conditions relevant to natural waters, oxidation of Mn(II) by superoxide can occur and lead to formation of Mn oxides. For organisms that oxidize Mn(II) by producing superoxide, these findings may also point to other microbially mediated processes, in particular enzymatic hydrogen peroxide degradation and/or production of organic ligand metabolites, that allow for Mn oxide formation.

Keywords: manganese oxidation, manganese oxides, superoxide, reactive oxygen species, Mn(III) complexes, organic ligands

Citation: Learman DR, Voelker BM, Madden AS and Hansel CM (2013) Constraints on superoxide mediated formation of manganese oxides. Front. Microbiol. 4:262. doi: 10.3389/fmicb.2013.00262

Received: 15 June 2013; Paper pending published: 26 July 2013;
Accepted: 15 August 2013; Published online: 03 September 2013.

Edited by:

Stephen Wiley Ragsdale, University of Michigan, USA

Reviewed by:

Jim K. Fredrickson, Pacific Northwest National Laboratory, USA
David Emerson, Bigelow Laboratory for Ocean Sciences, USA

Copyright © 2013 Learman, Voelker, Madden and Hansel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Colleen M. Hansel, Marine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, 266 Woods Hole Road, MS52, Woods Hole, MA 02543, USA e-mail: chansel@whoi.edu

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