Original Research ARTICLE

Front. Microbiol., 07 May 2014 | doi: 10.3389/fmicb.2014.00202

Pyoverdine synthesis by the Mn(II)-oxidizing bacterium Pseudomonas putida GB-1

Dorothy L. Parker1, Sung-Woo Lee2*, Kati Geszvain2, Richard E. Davis2, Christelle Gruffaz3, Jean-Marie Meyer3, Justin W. Torpey4 and Bradley M. Tebo2
  • 1Geosciences Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA
  • 2Division of Environmental and Biomolecular Systems, Oregon Health and Science University, Beaverton, OR, USA
  • 3Laboratoire de Génétique Moléculaire, Génomique et Microbiologie, Université de Strasbourg, Strasbourg, France
  • 4Biomolecular Mass Spectrometry Facility, Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA

When iron-starved, the Mn(II)-oxidizing bacteria Pseudomonas putida strains GB-1 and MnB1 produce pyoverdines (PVDGB-1 and PVDMnB1), siderophores that both influence iron uptake and inhibit manganese(II) oxidation by these strains. To explore the properties and genetics of a PVD that can affect manganese oxidation, LC-MS/MS, and various siderotyping techniques were used to identify the peptides of PVDGB-1 and PVDMnB1 as being (for both PVDs): chromophore-Asp-Lys-OHAsp-Ser-Gly-aThr-Lys-cOHOrn, resembling a structure previously reported for P. putida CFML 90-51, which does not oxidize Mn. All three strains also produced an azotobactin and a sulfonated PVD, each with the peptide sequence above, but with unknown regulatory or metabolic effects. Bioinformatic analysis of the sequenced genome of P. putida GB-1 suggested that a particular non-ribosomal peptide synthetase (NRPS), coded by the operon PputGB1_4083-4086, could produce the peptide backbone of PVDGB-1. To verify this prediction, plasmid integration disruption of PputGB1_4083 was performed and the resulting mutant failed to produce detectable PVD. In silico analysis of the modules in PputGB1_4083-4086 predicted a peptide sequence of Asp-Lys-Asp-Ser-Ala-Thr-Lsy-Orn, which closely matches the peptide determined by MS/MS. To extend these studies to other organisms, various Mn(II)-oxidizing and non-oxidizing isolates of P. putida, P. fluorescens, P. marincola, P. fluorescens-syringae group, P. mendocina-resinovorans group, and P. stutzerii group were screened for PVD synthesis. The PVD producers (12 out of 16 tested strains) were siderotyped and placed into four sets of differing PVD structures, some corresponding to previously characterized PVDs and some to novel PVDs. These results combined with previous studies suggested that the presence of OHAsp or the flexibility of the pyoverdine polypeptide may enable efficient binding of Mn(III).

Keywords: siderophore, pyoverdine, azotobactin, manganese oxidation, iron

Citation: Parker DL, Lee S-W, Geszvain K, Davis RE, Gruffaz C, Meyer J-M, Torpey JW and Tebo BM (2014) Pyoverdine synthesis by the Mn(II)-oxidizing bacterium Pseudomonas putida GB-1. Front. Microbiol. 5:202. doi: 10.3389/fmicb.2014.00202

Received: 18 March 2014; Accepted: 16 April 2014;
Published online: 07 May 2014.

Edited by:

Partha Basu, Duquesne University, USA

Reviewed by:

John Senko, The University of Akron, USA
Al Crumbliss, Duke University, USA

Copyright © 2014 Parker, Lee, Geszvain, Davis, Gruffaz, Meyer, Torpey and Tebo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Sung-Woo Lee, Division of Environmental and Biomolecular Systems, Oregon Health and Science University, 20000 NW Walker Rd., Beaverton, OR 97006, USA e-mail: sungwlz@gmail.com

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