Original Research ARTICLE

Front. Behav. Neurosci., 02 February 2012 | doi: 10.3389/fnbeh.2012.00003

Juvenile neurogenesis makes essential contributions to adult brain structure and plays a sex-dependent role in fear memories

Jesse D. Cushman1*†‡, Jose Maldonado2†‡, Eunice E. Kwon2†, A. Denise Garcia2†, Guoping Fan3†, Tetsuya Imura2†, Michael V. Sofroniew2†‡ and Michael S. Fanselow1†‡
  • 1Departments of Psychology and Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, USA
  • 2Department of Neurobiology, University of California Los Angeles, Los Angeles, CA, USA
  • 3Department of Human Genetics, University of California Los Angeles, Los Angeles, CA, USA

Postnatal neurogenesis (PNN) contributes neurons to olfactory bulb (OB) and dentate gyrus (DG) throughout juvenile development, but the quantitative amount, temporal dynamics and functional roles of this contribution have not been defined. By using transgenic mouse models for cell lineage tracing and conditional cell ablation, we found that juvenile neurogenesis gradually increased the total number of granule neurons by approximately 40% in OB, and by 25% in DG, between 2 weeks and 2 months of age, and that total numbers remained stable thereafter. These findings indicate that the overwhelming majority of net postnatal neuronal addition in these regions occurs during the juvenile period and that adult neurogenesis contributes primarily to replacement of granule cells in both regions. Behavioral analysis in our conditional cell ablation mouse model showed that complete loss of PNN throughout both the juvenile and young adult period produced a specific set of sex-dependent cognitive changes. We observed normal hippocampus-independent delay fear conditioning, but excessive generalization of fear to a novel auditory stimulus, which is consistent with a role for PNN in psychopathology. Standard contextual fear conditioning was intact, however, pre-exposure dependent contextual fear was impaired suggesting a specific role for PNN in incidental contextual learning. Contextual discrimination between two highly similar contexts was enhanced; suggesting either enhanced contextual pattern separation or impaired temporal integration. We also observed a reduced reliance on olfactory cues, consistent with a role for OB PNN in the efficient processing of olfactory information. Thus, juvenile neurogenesis adds substantively to the total numbers of granule neurons in OB and DG during periods of critical juvenile behavioral development, including weaning, early social interactions and sexual maturation, and plays a sex-dependent role in fear memories.

Keywords: adult neurogenesis, juvenile neurogenesis, postnatal neurogenesis, hippocampus, olfactory bulb, learning and memory, sex difference, fear conditioning

Citation: Cushman JD, Maldonado J, Kwon EE, Garcia AD, Fan G, Imura T, Sofroniew MV and Fanselow MS (2012) Juvenile neurogenesis makes essential contributions to adult brain structure and plays a sex-dependent role in fear memories. Front. Behav. Neurosci. 6:3. doi: 10.3389/fnbeh.2012.00003

Received: 03 December 2011; Accepted: 21 January 2012;
Published online: 02 February 2012.

Edited by:

Susan J. Sara, Collège du France, France

Reviewed by:

Marie H. Monfils, University of Texas at Austin, USA
Stefano Sensi, University of California Irvine, USA

Copyright: © 2012 Cushman, Maldonado, Kwon, Garcia, Fan, Imura, Sofroniew and Fanselow. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

*Correspondence: Jesse D. Cushman, Departments of Psychology and Psychiatry and Biobehavioral Sciences, University of California Los Angeles, 8548 Franz Hall, Los Angeles, CA 90095-1563, USA. e-mail: jcushman@ucla.edu; sofroniew@mednet.ucla.edu; fanselow@ucla.edu

Author Contributions: Jesse D. Cushman, Jose Maldonado, Michael V. Sofroniew, Michael S. Fanselow designed research. Jesse D. Cushman, Jose Maldonado, Eunice E. Kwon, A. Denise Garcia, Tetsuya Imura conducted research. Guoping Fan contributed materials. Jesse D. Cushman, Jose Maldonado, Eunice E. Kwon, A. Denise Garcia, Tetsuya Imura, Michael V. Sofroniew, Michael S. Fanselow analyzed data. Jesse D. Cushman, Jose Maldonado, Michael V. Sofroniew, Michael S. Fanselow wrote the paper.

These authors contributed equally.

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