Original Research ARTICLE

Front. Neuroinform., 30 December 2011 | doi: 10.3389/fninf.2011.00034

High-resolution fiber tract reconstruction in the human brain by means of three-dimensional polarized light imaging

Markus Axer1,2*, David Grässel1, Melanie Kleiner1, Jürgen Dammers1, Timo Dickscheid1, Julia Reckfort1,2, Tim Hütz1, Björn Eiben1, Uwe Pietrzyk1,2, Karl Zilles1,3 and Katrin Amunts1,4
  • 1 Institute of Neuroscience and Medicine (INM-1, INM-2, INM-4), Research Centre Jülich and Jülich Aachen Research Alliance, Jülich, Germany
  • 2 Department of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany
  • 3 C. and O. Vogt Institute for Brain Research, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany
  • 4 Department of Psychiatry, Psychotherapy and Psychosomatics, RWTH Aachen University, Aachen, Germany

Functional interactions between different brain regions require connecting fiber tracts, the structural basis of the human connectome. To assemble a comprehensive structural understanding of neural network elements from the microscopic to the macroscopic dimensions, a multimodal and multiscale approach has to be envisaged. However, the integration of results from complementary neuroimaging techniques poses a particular challenge. In this paper, we describe a steadily evolving neuroimaging technique referred to as three-dimensional polarized light imaging (3D-PLI). It is based on the birefringence of the myelin sheaths surrounding axons, and enables the high-resolution analysis of myelinated axons constituting the fiber tracts. 3D-PLI provides the mapping of spatial fiber architecture in the postmortem human brain at a sub-millimeter resolution, i.e., at the mesoscale. The fundamental data structure gained by 3D-PLI is a comprehensive 3D vector field description of fibers and fiber tract orientations – the basis for subsequent tractography. To demonstrate how 3D-PLI can contribute to unravel and assemble the human connectome, a multiscale approach with the same technology was pursued. Two complementary state-of-the-art polarimeters providing different sampling grids (pixel sizes of 100 and 1.6 μm) were used. To exemplarily highlight the potential of this approach, fiber orientation maps and 3D fiber models were reconstructed in selected regions of the brain (e.g., Corpus callosum, Internal capsule, Pons). The results demonstrate that 3D-PLI is an ideal tool to serve as an interface between the microscopic and macroscopic levels of organization of the human connectome.

Keywords: connectome, human brain, method, polarized light imaging, PLI, U-fiber, systems biology, white matter

Citation: Axer M, Grässel D, Kleiner M, Dammers J, Dickscheid T, Reckfort J, Hütz T, Eiben B, Pietrzyk U, Zilles K and Amunts K (2011) High-resolution fiber tract reconstruction in the human brain by means of three-dimensional polarized light imaging. Front. Neuroinform. 5:34. doi: 10.3389/fninf.2011.00034

Received: 18 March 2011; Accepted: 08 December 2011;
Published online: 30 December 2011.

Edited by:

Claus Hilgetag, Jacobs University Bremen, Germany

Reviewed by:

Suzanne Haber, University of Rochester, USA
Marc Tittgemeyer, Max-Planck-Institute for Neurological Research, Germany

Copyright: © 2011 Axer, Grässel, Kleiner, Dammers, Dickscheid, Reckfort, Hütz, Eiben, Pietrzyk, Zilles and Amunts. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

*Correspondence: Markus Axer, Institute of Neuroscience and Medicine, INM-1, Research Centre Jülich, D-52425 Jülich, Germany. e-mail: m.axer@fz-juelich.de

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