This article is part of the Research Topic The neuronal functions of EF-hand Ca(2+)-binding proteins

Original Research ARTICLE

Front. Mol. Neurosci., 08 March 2012 | doi: 10.3389/fnmol.2012.00028

Synergetic effect of recoverin and calmodulin on regulation of rhodopsin kinase

Ilya I. Grigoriev1, Ivan I. Senin1*, Natalya K. Tikhomirova1, Konstantin E. Komolov1,2, Sergei E. Permyakov3, Evgeni Yu. Zernii1*, Karl-Wilhelm Koch2 and Pavel P. Philippov1
  • 1 Department of Cell Signaling, A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
  • 2 Biochemistry Group, Department of Biology and Environmental Sciences, University of Oldenburg, Oldenburg, Germany
  • 3 Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia

Phosphorylation of photoactivated rhodopsin by rhodopsin kinase (RK or GRK1), a first step of the phototransduction cascade turnoff, is under the control of Ca2+/recoverin. Here, we demonstrate that calmodulin, a ubiquitous Ca2+-sensor, can inhibit RK, though less effectively than recoverin does. We have utilized the surface plasmon resonance technology to map the calmodulin binding site in the RK molecule. Calmodulin does not interact with the recoverin-binding site within amino acid residues M1-S25 of the enzyme. Instead, the high affinity calmodulin binding site is localized within a stretch of amino acid residues V150-K175 in the N-terminal regulatory region of RK. Moreover, the inhibitory effect of calmodulin and recoverin on RK activity is synergetic, which is in agreement with the existence of separate binding sites for each Ca2+-sensing protein. The synergetic inhibition of RK by both Ca2+-sensors occurs over a broader range of Ca2+-concentration than by recoverin alone, indicating increased Ca2+-sensitivity of RK regulation in the presence of both Ca2+-sensors. Taken together, our data suggest that RK regulation by calmodulin in photoreceptor cells could complement the well-known inhibitory effect of recoverin on RK.

Keywords: rhodopsin kinase, calmodulin, recoverin, phosphorylation, surface plasmon resonance

Citation: Grigoriev II, Senin II, Tikhomirova NK, Komolov KE, Permyakov SE, Zernii EY, Koch K-W and Philippov PP (2012) Synergetic effect of recoverin and calmodulin on regulation of rhodopsin kinase. Front. Mol. Neurosci. 5:28. doi: 10.3389/fnmol.2012.00028

Received: 05 January 2012; Accepted: 17 February 2012;
Published online: 08 March 2012.

Edited by:

Michael R. Kreutz, Leibniz-Institute for Neurobiology, Germany

Reviewed by:

Karl Heinz Braunewell, Southern Research Institute, USA
Jacques Haiech, University of Strasbourg, France

Copyright: © 2012 Grigoriev, Senin, Tikhomirova, Komolov, Permyakov, Zernii, Koch and Philippov. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

*Correspondence: Ivan I. Senin and Evgeni Yu. Zernii, Department of Cell Signaling, A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie Gory 1, Building 40, Moscow 119992, Russia. e-mail: senin@belozersky.msu.ru; zerni@belozersky.msu.ru

Present address: Konstantin E. Komolov, Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA.

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