GABAergic activities control spike timing- and frequency-dependent long-term depression at hippocampal excitatory synapses

Introduction

GABAergic interneuronal network activities regulate a variety of neural functions, such as modulation of activity-dependent synaptic plasticity (Meredith et al., 2003 ), i.e., long-term potentiation (LTP) and depression (LTD), and serve as a driving force for the hippocampal θ and γ oscillations important for processing learning and memory (Buzsáki, 2002 ; Csicsvari et al., 2003 ; Lisman et al., 2005 ). GABA functions as a major inhibitory neurotransmitter at both excitatory (glutamatergic) and inhibitory (GABAergic) neurons in the central nervous system by activating GABA_A receptors (GABA_ARs) and GABA_B receptors (GABA_BRs) in both pre- and postsynaptic cells (Connors et al., 1988 ; Davies and Collingridge, 1996 ). However, the inhibitory function of GABA on postsynaptic GABA_ARs depends on the state of the Cl⁻ ion reversal potential relative to the resting membrane potential (Staley et al., 1995 ). For example, in the hippocampus, GABAergic inhibition induced by stimuli at the α/β (low, 10 Hz) frequency shifts to excitation when stimuli occur at the γ (high, 40 Hz) frequency as a consequence of intracellular Cl⁻ ion ([Cl⁻]_i) accumulation in postsynaptic CA1 pyramidal cells (Staley et al., 1995 ; Bracci et al., 2001 ). In addition, GABA inhibition through presynaptic GABA_BRs, which are linked to G-protein-coupled inwardly rectifying K⁺ channels (GIRK), imposes different frequency dependencies at excitatory and inhibitory presynaptic terminals that are effective, respectively, above the α/β frequency (Ohliger-Frerking et al., 2003 ) and near the θ frequency (Davies and Collingridge, 1996 ).

In hippocampal neural networks, different populations of GABAergic interneurons (Maccaferri et al., 2000 ; Klausberger and Somogyi, 2008 ) contribute to postsynaptic feedforward (FF) and feedback (FB) inhibition at CA1 pyramidal cells (Pouille and Scanziani, 2001 , 2004 ) and to presynaptic inhibition at excitatory and inhibitory presynaptic terminals to CA1 pyramidal cells. However, it is unknown how these distinct GABA actions affect excitatory synaptic gains, either differentially or synergistically. Depending on the precise timing of pre- and postsynaptic neuronal spikes, repetitive spiking reliably induces either LTP or LTD, collectively known as spike-timing-dependent plasticity (STDP), in hippocampal neurons in culture (Bi and Poo, 1998 ; Debanne et al., 1998 ), in hippocampal or cortical neurons in slices (Markram et al., 1997 ; Feldman, 2000 ; Nishiyama et al., 2000 ; Sjöström et al., 2001 ; Froemke and Dan, 2002 ; Woodin et al., 2003 ), and in neurons of the tectum in vivo (Zhang et al., 1998 ). Previously, we showed that at hippocampal Schaffer collateral (SC)-CA1 pyramidal cell excitatory synapses, STDP induced by stimuli at 5 Hz (θ frequency) is composed of two distinct LTD time intervals (−LTD: −28 to −16 ms and +LTD: +15 to +20 ms) that flank an LTP time interval (−2 to +15 ms) (Nishiyama et al., 2000 ). Interestingly, this LTP/LTD time interval cycle is completed within 25 ms at 40 Hz (γ frequency), similar to the time course of the γ oscillation in vivo (Csicsvari et al., 2003 ), which hippocampal GABAergic interneuronal networks are believed to regulate (Whittington et al., 1995 ; Bartos et al., 2007 ; Cardin et al., 2008 ; Sohal et al., 2008 ). Also, repetitive stimuli at SC inputs persistently alter synaptic efficacy at GABAergic inputs to CA1 pyramidal cells (Woodin et al., 2003 ; Chevaleyre and Castillo, 2004 ; Lamsa et al., 2005 ; Gibson et al., 2008 ). We, therefore, monitored synaptic efficacy at CA1 pyramidal cells to investigate how these dynamic patterns of GABAergic interneuronal inputs regulate spike timing- and frequency-dependent STDP at excitatory synapses (e-STDP).

Materials and Methods

Hippocampal Slice Preparation

Hippocampal slices were prepared by a standard procedure (Nishiyama et al., 2000 ). Male Sprague-Dawley rats (26- to 35-day old) were anaesthetized and decapitated. Right hippocampi were dissected rapidly and placed in a gassed (95% O₂–5% CO₂) extracellular solution containing (in mM) 124 NaCl, 3 KCl, 2.6 CaCl₂, 1.3 MgSO₄, 1.25 NaH₂PO₄, 22 NaHCO₃ and 10 D-glucose at 10°C. Transverse slices of 500-μm thickness were cut with a rotor tissue slicer (Dosaka, DTY7700) and maintained at room temperature (23–26°C) in an incubation chamber for at least 2 h. For experiments, individual slices were transferred to a submersion recording chamber and perfused continuously with extracellular solution (4.0∼4.5 ml/min) at room temperature. Experiments using a K⁺-based internal recording solution (see below) were performed at 30–33°C. To prevent epileptiform activity, the CA3 region was removed in experiments that used GABA and muscarinic receptor antagonists.

Whole-Cell Recording

Whole-cell recordings were made in the CA1 cell body layer with the “blind” patch clamp method, using an Axopatch 200B amplifier (Axon Inst.). Test stimuli were applied at 0.05 Hz and alternated between two non-overlapping SC inputs using bipolar electrodes (MCE-100, RMI) under voltage-clamp (V_c = −80 mV) [except when inhibitory postsynaptic currents (IPSCs) were measured] to evoke excitatory postsynaptic currents (EPSCs). Both stimulating electrodes were positioned at the stratum radiatum at least 500 μm distant from the recording electrode to avoid stimulation of monosynaptic (direct) inhibitory inputs. The non-overlap of the two inputs was confirmed by applying alternate paired-pulse test stimuli (at 50-ms intervals) to demonstrate the absence of cross facilitation between the two inputs. Constant current pulses (amplitude, 6–14 μA; duration, 300 μs) evoked EPSCs with amplitudes of 100–200 pA. These current intensities were at least three fold smaller than the stimulation currents that evoke population spikes. Following a stable recording period of about 12 (K⁺-based internal recording solution) or 15 (Cs⁺-based internal recording solution) min, the recording was switched to current clamp and a train of stimuli at a frequency of 5 Hz was delivered to one of the two inputs for either 20 s (K⁺-based) or 16 s (Cs⁺-based). Each presynaptic stimulus was paired with the injection of a spike-form (K⁺-based: 2 nA for 2 ms at the peak, I_max) or square (Cs⁺-based: 2 nA for 2 ms) depolarizing current pulse into the postsynaptic neuron to initiate spiking at various time intervals. The spike-form depolarizing currents (t in ms): I_max•10•t, 0 ≤ t < 0.1; I_max, 0.1 ≤ t < 2.1; I_max•[(1−(0.8/1.5)•(t−2.1)], 2.1 ≤ t < 3.6; I_max•[0.2−(0.12/2)•(t−3.6)], 3.6 ≤ t < 5.6; I_max•0.08•[1−(1/2.5)•(t−5.6)], 5.6 ≤ t < 8.1 were designed to reduce the jitter of postsynaptic spikes but not to affect the spike decay time. Depolarization-induced suppression of inhibition (DSI) was induced by changing V_c from −80 to 0 mV for 2–3 s, followed by the application of the spike-timing protocol (at 5-, 12- and 25-Hz for 16, 6.7 and 3.2 s, respectively) for various time intervals after 5–10 s. A voltage-step of +90 mV from the holding potential (at −80 mV) was applied for 40 ms every 60 s to visualize feedforward (FF)-IPSCs during the concurrent monitoring of EPSC/FF-IPSC. A 40-ms voltage-step was chosen to avoid the induction of DSI (estimated to be less than 1% according to Lenz and Alger, 1999 ) but was long enough to detect peak currents of disynaptic FF-IPSCs. During this voltage-step, EPSC was masked because the commanding potential (i.e., +10 mV) was approximately equivalent to the EPSC reversal potential. The identity of FF-IPSC was confirmed as a result of its abolition by bath-applied gabazine (10–100 μM) and kynurenic acid (5 mM). Data were filtered at 2 kHz and digitized at 10 kHz. Patch electrodes were pulled from borosilicate glass (1.2-mm O.D.) and had a resistance of 3.5∼6 MΩ. Pipettes were filled with a solution containing (in mM): 120 K-gluconate, 10 KMeSO₃, 10 KCl, 0.075 BAPTA, 20 HEPES, 4 Mg-ATP, 2 Na2-ATP, pH adjusted to 7.35 with KOH. For experiments that required sustained postsynaptic depolarization, a Cs⁺-based internal solution was used that contained (in mM): 130 cesium methanesulphonate (CsMeSO₃), 10 tetraethylammonium (TEA) chloride (Cl⁻), 0.25 1,2-bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA), 10 N-[1-hydroxyethyl]-piperazine-N’-[2-ethanesulphonic acid] (HEPES), 2 Mg-ATP, 2 Na₂-ATP, pH adjusted to 7.35 with CsOH,. The series resistance (typically 10–14 MΩ) was compensated 50–80% and was monitored throughout the experiment using a −5 mV step command. Cells that showed an unstable series resistance (>20% changes after the spike-timing protocol) were not used. To monitor monosynaptic IPSCs, 40 μM CNQX and 50 μM D(–)-2-amino-5-phosphonovaleric acid (AP5) were present in the extracellular solution and CsCl instead of CsMeSO₃ was used intracellularly while the cells were held at −70 mV. Drugs (bicuculline, kynurenic acid, gabazine, CNQX, AP5 and phaclofen) were purchased from either RBI or Tocris. CGP35348 was a generous gift from Novartis.

Results

Single, Pre- and Postsynaptic Spiking at the θ Frequency Recruits Both Feedforward and Feedback Postsynaptic GABA_AR-Mediated Inhibition

We performed whole-cell recordings at CA1 pyramidal cells in hippocampal slices (Figure 1 A) in which the GABAergic networks are well defined (Figure 1 B) (Maccaferri et al., 2000 ; Pouille and Scanziani, 2001 , 2004 ; Klausberger and Somogyi, 2008 ). We stimulated SC inputs in the stratum radiatum at 100–150 μm away from the stratum pyramidale (SP, somatic layer) to evoke both EPSCs and FF-IPSCs in 500 μm thick slices. We used a CsMeSO₃-based (Cs⁺-based) internal solution, which blocks GIRKs, to isolate the effects of postsynaptic GABA_AR-mediated inhibition from those of postsynaptic GABA_BR-mediated inhibition. In this configuration, we found that substantially more GABAergic inputs relative to glutamatergic inputs were retained than in thinner (300 and 400 μm) slices (Figure 1 C); the FF-IPSC amplitude was 1045 ± 44 pA when the EPSC amplitude was 301 ± 8 pA (±sem, n = 408; differences between commanding and reversal potentials were ca. 90 mV for both FF-IPSCs and EPSCs) (Megias et al., 2001 ). Consistent with the idea that monosynaptic EPSCs and disynaptic FF-IPSCs normally share the same population of SC inputs in our experimental paradigm, the magnitudes of both EPSCs and FF-IPSCs changed in positive correlation with the magnitudes of presynaptic stimuli at SC inputs (Figures 1 C,D). Weak, superlinearly- increased FF-IPSC amplitudes, greater than those of EPSCs, were observed when strong stimuli were applied to SC inputs (i.e., EPSC amplitudes >300 pA; Figure 1 D, bottom). This superlinearity, the mechanism for which is unknown, may help to facilitate the failure of LTP induction at strong inputs, which has been demonstrated in cultured hippocampal neurons (Bi and Poo, 1998 ) and is believed to maintain gain levels of the entire network activity (van Rossum et al., 2000 ). It is noteworthy that as presynaptic stimulation was decreased, FF-IPSCs disappeared at a level at which EPSCs of ∼70 pA remained (see Figure 1 D inset), indicating that e-STDP can be examined in the absence of functional FF-IPSCs to individually-recorded pyramidal cells.

FIGURE 1

Figure 1. Repetitive, single pre- and postsynaptic spiking recruits both FF and FB postsynaptic GABA_AR-mediated inhibition in hippocampal CA1 pyramidal cells. (A) Experimental paradigm depicting the positions of stimulating and recording electrodes. Whole-cell recordings were made in the CA1 cell body and the test stimuli were applied at the stratum radiatum at least 500 μm distant from the recording electrode. (B) Schematic diagram of neuronal networks involved in the induction of STDP in CA1 pyramidal cells. Locations of excitatory (e) and inhibitory (i) inputs and GABA receptors are indicated. (C) Procedure for concurrent measurement of EPSCs and FF-IPSCs (top) and summary of their amplitudes (bottom). FF-IPSC is detected (top middle) with the use of voltage-steps (top upper) and presynaptic stimulation (pre-stim). The magnitude of FF-IPSC (top lower, black) is calculated by subtracting the voltage-step alone (top middle, black) from the pre-stimulation value (top middle, blue). The voltage-step at +10 mV was applied for 40 ms to prevent the induction of DSI (less than 1%; Lenz and Alger, 1999 ). The FF-IPSC was confirmed by GABA_A blockade with bicuculline (20 μM, top middle and lower, red). The ratio of FF-IPSCs to EPSCs increased as the slice thickness increased. Data represent the mean EPSC/FF-IPSC (±sem). Traces: FF-IPSC in 500-μm (red), 400-μm (gray) and 300-μm (black) thick slices. The (number) indicates the total number of trials. Significant differences from corresponding data in 500-μm thick slices are indicated (“*” p < 0.05 and “**” p < 0.01; Mann–Whitney U test). (D) The amplitude of FF-IPSC is proportional to the EPSC amplitude. When the EPSC level is <70 pA, there is no FF-IPSC (see red line in inset). The EPSC amplitude normally used in this study is indicated (blue line in inset). (E) Prominent FB postsynaptic GABA_AR-mediated inhibition is recruited by repetitive 5-Hz postsynaptic spiking in CA1 pyramidal cells. Postsynaptic spiking was induced by depolarizing current injections (6 nA, 2 ms, 20 pulses at 5 Hz) while postsynaptic CA1 pyramidal cells were held at −100 mV in a current-clamp configuration using a Cs⁺-based internal recording solution. The top left panel shows the postsynaptic membrane potentials monitored when FB postsynaptic GABA_AR-mediated inhibition was blocked by either kynurenic acid (5 mM) or gabazine (10 μM). The control, in which GABA_AR was not blocked, showed a feedback inhibition-inclusive membrane potential. Subtraction analysis (bottom left) revealed a membrane potential resulting from FB postsynaptic GABA_AR-mediated inhibition. Average FB-IPSP (right).

To ascertain whether FB postsynaptic GABA_AR-mediated inhibition occurred during the θ frequency (5 Hz) spike-timing protocol, we injected postsynaptic spike-inducing depolarizing currents (6 nA for 2 ms) through the whole-cell recording electrodes for 20 pulses (at 5 Hz) in the absence of presynaptic stimuli. The recorded cell was held at −100 mV under the current clamp to avoid sustained depolarization after spikes in the absence of K⁺ channel function. Feedback postsynaptic GABA_AR-mediated membrane hyperpolarization was measured as differential potentials in which the spike afterpotential (averaged between the 15th and 20th spikes) in the presence of either gabazine (10–100 μM), a GABA_AR antagonist, or kynurenic acid (5 mM), a glutamate receptor antagonist (to eliminate disynaptic responses), was subtracted from that in the absence of antagonists (Figure 1 E). We observed a FB postsynaptic GABA_AR-mediated hyperpolarizing effect of ca. −34 mV when cell membrane potentials were at ca. −40 mV, demonstrating the occurrence of functional FB postsynaptic GABA_AR-mediated inhibition. Thus, our experimental paradigm is a unique method to investigate differential GABAergic effects (e.g., pre- vs. postsynaptic, GABA_AR- vs. GABA_BR-mediated and FF vs. FB) on the induction of e-STDP.

e-STDP Induced at the θ Frequency is Independent of Postsynaptic GABA_BR-Mediated Inhibition

Blocking postsynaptic K⁺ channel activity, including that linked to GABA_BR function, may also affect the spike-timing dependence of e-STDP. We, therefore, examined the timing-dependent feature of e-STDP using a K-gluconate-based (control) internal solution in the recording pipette. We applied SC stimulation that evoked EPSCs of amplitude >70 pA to ensure the presence of functional FF-IPSCs (Figure 1 D). When presynaptic stimuli at SC-CA1 synapses were paired with postsynaptic spike-inducing depolarizing currents with a time interval of +5 ms at 5 Hz for 20 s, we observed input-specific e-LTP (Figure 2 A), which was comparable to that induced by a 16-s spike-timing protocol (at positive time interval) in the Cs⁺-based internal solution (Figure 2 A). That a comparable LTP was induced is likely attributable to the fact that activated postsynaptic sites were close enough to the soma to receive back-propagating action potentials due to the absence of prominent A-type K⁺ channel activity (Hoffman et al., 1997 ). These conditions permit repetitive, single pre- and postsynaptic spiking to induce e-LTP (Nishiyama et al., 2000 ).

FIGURE 2

Figure 2. e-STDP is induced at the θ frequency independently of postsynaptic GABA_BR function at SC-CA1 synapses. (A) Time course of changes in EPSC amplitudes induced by the LTP-timing protocol in homosynaptic, activated inputs (left) and heterosynaptic, non-activated inputs (right). Input-specific e-LTP was observed in K⁺-based (control, Ctrl) and Cs⁺-based internal recording solution. Data represent the normalized mean EPSC (±sem). (B) Action potentials recorded at the soma with the LTP time intervals in the presence or absence of K⁺ channel activities. Membrane potential (Vm) changes in either K⁺-based (black) or Cs⁺-based (red) internal solution (left). The average spike amplitude (right upper) and the half maximum width (right lower) were not as significantly different between the two experimental conditions as reported previously (Wittenberg and Wang, 2006 ). This may be due to extensive postsynaptic GABA_AR-mediated inhibition in our preparation. Significant difference from the control is indicated (“**” p < 0.01; Mann–Whitney U test). (C) Membrane potentials (Vm) controlled by GABA_AR-mediated inhibition. Stable Vm was observed in 500-μm (black), but not in 300-μm (gray) thick hippocampal slices in which elimination of postsynaptic GABA_AR-mediated inhibition by bicuculline (20 μM) treatment caused depolarization (red). Inset: twice magnified view. (D) Summary of normalized EPSC changes in homosynaptic (left) and heterosynaptic (right) inputs. A similar timing dependence of e-STDP was observed in both K⁺-based (Ctrl) and Cs⁺-based (–post-GABA_B; without postsynaptic GABA_BR-mediated inhibition) internal solution. e-LTD at both negative and positive time intervals propagated to heterosynaptic, non-activated synapses (right). Gray areas indicate e-LTD time intervals in the K⁺-based internal solution. Data recorded in the Cs⁺-based internal solution is adapted from (Nishiyama et al., 2000 ).

We also compared the postsynaptic spike wave forms during the LTP-timing protocol in the Cs⁺-based internal solution with those in the K⁺-base internal solution (Figure 2 B). In the Cs⁺-based internal solution, the spikes evoked by the postsynaptic depolarizing currents were ca. 50% broader (half-maximal width of 3.1 ± 0.3 ms, n = 18) than in the K⁺-based solution. However, this spike width is noticeably smaller than that reported previously in thinner (300 μm) slices (Wittenberg and Wang, 2006 ). Moreover, membrane potential changes recorded in the postsynaptic cell during the LTP-timing protocol were stably maintained, even when the Cs⁺-based internal solution was used, in 500-μm thick slices (Figure 2 C). In contrast, a strong depolarization was observed when we applied the same spike-timing protocol in thinner (i.e., 300-μm thick) slices or slices (500-μm thick) treated with 20 μM bicucullin, which eliminated postsynaptic GABA_AR-mediated inhibition (Figure 2 C). Collectively, the observations of relatively small spike width and stable postsynaptic membrane potential in the Cs⁺-based internal solution support the presence of prominent postsynaptic GABA_AR-mediated inhibition that accelerated spike after-repolarization.

We systematically examined the spike-timing dependence of e-STDP at the θ frequency. As shown in Figure 2 D, we observed two distinct time windows for the induction of e-LTD, −31 to −18 ms (–LTD) and +18 to +34 ms (+LTD), that flanked single time intervals of +3 to +9 ms for the induction of e-LTP. Moreover, the induction of e-LTD for both negative and positive time intervals at activated synapses was associated with heterosynaptic e-LTD at non-activated synapses. Although a broader time window for +LTD (20 ms duration) was observed in the K⁺-based internal solution, the timing dependence of this e-STDP was similar to that in the Cs⁺-based internal solution (in the absence of postsynaptic GABA_BR-mediated inhibition, Figure 2 D; modified from Nishiyama et al., 2000 by the addition of new data points). This suggests that postsynaptic GABA_BR-mediated inhibition-linked GIRKs and other K⁺ channel activities, including that of A-type K⁺ channels, are not essential determinants of the spike-timing dependence of e-STDP induced at the θ frequency.

Postsynaptic GABA_AR-Mediated Inhibition Controls the Spike-Timing Dependence of e-LTD

The induction of STDP depends on the pattern of postsynaptic Ca²⁺ elevation ( Magee and Johnston, 1997 ; Nishiyama et al., 2000 ; Sjöström and Nelson, 2002 ; Dan and Poo, 2006 ; Nevian and Sakmann, 2006 ; Caporale and Dan, 2008 ), which results from interactions between dendritic back-propagating action potentials and excitatory postsynaptic potentials (EPSPs) (Koester and Sakmann, 1998 ; Schiller et al., 1998 ; Stuart and Häusser, 2001 ). Since dendritic action potentials are regulated by FF and FB postsynaptic GABA_AR-mediated inhibition (Buzsáki et al., 1996 ; Tsubokawa and Ross, 1996 ; Pouille and Scanziani, 2001 ), we examined whether postsynaptic GABA_AR-mediated inhibition affects the spike-timing dependence of e-STDP induced at the θ frequency. We first investigated the impact of postsynaptic GABA_AR-mediated inhibition on the induction of e-LTD (–LTD; Figure 2 D), in the K⁺-based internal solution as a control. To eliminate postsynaptic GABA_AR-mediated inhibition, gabazine (5 μM), a GABA_AR antagonist (Pouille and Scanziani, 2004 ), was administrated in the bath a few minutes before the spike-timing protocol and remained throughout the e-LTD induction protocol. As shown in Figure 3 A, e-LTD was abolished in the presence of gabazine, demonstrating that GABA_AR activity is required for the induction of e-LTD. To confirm that postsynaptic GABA_AR-mediated membrane hyperpolarization was responsible for the e-LTD induction, the recorded cells were held at −85 mV (in current clamp) while postsynaptic GABA_AR-mediated inhibition was blocked by bath-applied gabazine. The reversal potential of GABA_AR was −73.8 ± 3.1 mV (n = 4), when the Cs⁺-based internal solution was used. Application of hyperpolarizing currents to the recorded cells restored the induction of e-LTD, which, unlike in the control, was expressed immediately after the spike-timing protocol (Figure 3 A). The slow onset of e-LTD in the presence of GABA_AR function may be due to robustly enhanced FF-IPSCs immediately after the spike-timing protocol (see Figure 5 C), the FF-IPSC component of which is expected to be 14.5 ± 0.8% of the EPSC amplitude (n = 4) before the induction protocol.

FIGURE 3

Figure 3. Postsynaptic GABA_AR-mediated inhibition controls the spike-timing dependence of e-LTD at the θ frequency. (A) Bath application of gabazine (5 μM, yellow bars) abolished e-LTD (time intervals: −24 to −17 ms), whereas hyperpolarization (hyperpol) of postsynaptic cells restored e-LTD. (B) Brief postsynaptic depolarization (arrow; dep, 3 s) induced DSI at SC inputs. (C) DSI (red bar) induction immediately preceding the spike-timing protocol (black arrow) abolished e-LTD (time intervals: −24 to −22 ms), whereas DSI applied 3 min before (gray arrow) had no effect on the e-LTD. Data represent normalized mean EPSCs/IPSCs (±sem). Sample traces of membrane potentials during the spike-timing protocol (top, in A,C) and of IPSCs (in B)/EPSCs (bottom, in A,C) before (1) and after (2) the induction protocols. Significant differences from corresponding controls are indicated (“**” p < 0.01; Mann–Whitney U test). (D) Summary of time windows for e-STDP in the presence (filled red circle) and absence (filled gray circle) of DSI. Gray areas show LTD time intervals in the absence of DSI.

The bath application of gabazine would eliminate total GABA_AR function in hippocampal networks. Therefore, to test the effects of postsynaptic GABA_AR-mediated inhibition specifically on the recorded cells, we applied the method of depolarization-induced suppression of inhibition (DSI, Lenz and Alger, 1999 ) by clamping the recorded cell at 0 mV using the Cs⁺-based internal solution. Since stimulation at the stratum oriens/alveus has been used to demonstrate DSI (Lenz and Alger, 1999 ), we examined the effects of DSI on IPSCs evoked by stimuli at the stratum radiatum. Application of a 2–3 s depolarization (from −70 to 0 mV) to the recorded postsynaptic CA1 pyramidal cell induced DSI (43 ± 6% of the control, n = 5) for about 2 min (Figure 3 B), similarly as previously reported (Lenz and Alger, 1999 ). In this particular experiment, we applied ca. two to three fold stronger stimuli (amplitude, 20–40 μA; duration, 300 μs) to evoke monosynaptic IPSCs of ca. 70 pA. When the DSI procedure was applied 5–10 s before the application of the spike-timing protocol at the θ frequency, induction of e-LTD within the time interval of −24 to −22 ms (–LTD) was eliminated (Figure 3 C). In contrast, when the DSI procedure was applied 3 min before the same spike-timing protocol, by which time FF-IPSCs should have recovered to normal levels from the DSI (Figure 3 B), the induction of e-LTD was successfully restored to control levels (Figure 3 C). The postsynaptic Ca²⁺ increase caused by the DSI procedure, which is known to last for only a few seconds (Isokawa and Alger, 2006 ), is unlikely to have overlapped with that subsequently induced by the spike-timing protocol (applied 5–10 s later).

The effects of DSI on e-STDP induced at various time intervals (from −92 to +94 ms) were also examined. As shown in Figure 3 D, the DSI did not affect significantly the magnitude of e-LTP induced by the LTP-timing protocol (time interval at +5 ms). In contrast, DSI not only eliminated e-LTD induction at both the –LTD and +LTD time intervals, but induced the most robust e-LTP at the original +LTD time intervals (Figure 3 D). This might be due to the induction of higher NMDA receptor-mediated currents that coincided with postsynaptic spikes (Vargas-Caballero and Robinson, 2004 ). Moreover, reduction of postsynaptic GABA_AR-mediated inhibition to approximately one half by the DSI resulted in two effects on the STDP time window for e-LTP induction: First, the original window of −2 to +15 ms was expanded to −15 to +20 ms, and second, additional LTP time windows appeared at −100 to −55 ms and at +45 to +100 ms (Figure 3 D). This temporal feature of e-LTP induction may be consistent with the idea that DSI biases the STDP learning rule toward LTP. In the presence of DSI, we observed two distinct time windows (−55 to −15 ms and +20 to +45 ms) in which no e-LTP could be induced. The absence of e-LTP at −55 to −15 ms may have been due to the overlapping slow component of spike afterhyperpolarization, which was sensitive to blockade of inositol 1,4,5-trisphosphate receptors (unpublished data). The current study, however, cannot explain the absence of e-LTP (and slight e-LTD) at the intervals of +20 to +45 ms. Taken together, these results demonstrate the essential role of postsynaptic GABA_AR-mediated inhibition as a critical determinant for the timing dependence of the e-STDP induction protocol at the θ frequency.

Feedback Postsynaptic GABA_AR-Mediated Inhibition Causes e-LTD to Enforce Cooperative e-LTP

As demonstrated above, prominent FB postsynaptic GABA_AR-mediated inhibition was induced by 5-Hz postsynaptic spiking (Figure 1 E), whereas FF postsynaptic GABA_AR-mediated inhibition was diminished as the intensity of presynaptic stimulation at SC inputs was reduced to a level that induced EPSCs of amplitudes <70 pA (red line in Figure 1 D inset). We, therefore, examined the effect of FB inhibition on e-STDP in the absence of functional FF inhibition by stimulating a smaller population of SC inputs that evoked small EPSCs (<70 pA), using the Cs⁺-based recording solution. Unexpectedly, we found that the LTP-timing protocol (spiking intervals of +4 to +6 ms) induced e-LTD instead of e-LTP (Figures 4 A,B), consistent with the requirement of cooperativity among multiple, coincident inputs for the induction of e-LTP (Bliss and Collingridge, 1993 ). Moreover, when FB postsynaptic GABA_AR-mediated inhibition was suppressed by DSI, this e-LTD became a robust e-LTP, similar to that induced at inputs of amplitudes >70 pA (Figures 4 A,B). Associative e-LTP can be induced at a single SC input to CA1 pyramidal cells by the pairing protocol (postsynaptic depolarization to 0 mV with presynaptic stimuli of 200 pulses at 1 Hz) in the absence of GABA_AR-mediated inhibition (Petersen et al., 1998 ). These results, therefore, suggest that FB postsynaptic GABA_AR-mediated inhibition not only prevents e-LTP induction at LTP time intervals (i.e., enforcing the requirement of cooperativity), but also causes the induction of e-LTD (i.e., gating for the induction of e-LTD). Similar e-LTD induction with the LTP-timing protocol has been reported in layer V cortical neurons at inputs from layer II/III neurons with stimuli at 50 Hz (Sjöström and Häusser, 2006 ). This e-LTD induction by a high-frequency (>50 Hz) protocol may be attributable to the difference of the threshold frequency of the spiking required to evoke FB postsynaptic GABA_AR-mediated inhibition in these cortical neurons (Silberberg and Markram, 2007 ). Thus, this gating function of e-LTD by FB inhibition may be a universal rule for e-STDP. In contrast, e-LTD induction at the negative time intervals (–LTD) did not show this cooperative requirement (Figures 4 C,D). An EPSC amplitude of 70 pA normally represents the co-activation of 10–20 synapses, as estimated from the average mini-EPSC amplitude of 3–5 pA (Kato et al., 1994 ), which may be capable of generating local dendritic Ca²⁺ spikes (Schiller et al., 2000 ). Therefore, intricate dendritic processing (e.g., integration or competition) of information according to the learning rule based on local spiking and that based on the back-propagating action potential (Jarsky et al., 2005 ; Rumsey and Abbott, 2006 ) may occur during the spike-timing protocol at the θ frequency.

FIGURE 4

Figure 4. Feedback postsynaptic GABA_AR-mediated inhibition causes e-LTD that enforces the requirement of cooperative e-LTP. (A,B) e-LTD is induced by the LTP-timing protocol when the initial EPSC amplitudes are <70 pA. DSI converts this e-LTD to e-LTP to the same extent as that induced normally at EPSC inputs of amplitude >70 pA. (C,D) e-LTD is induced to a similar extent independent of the initial EPSC amplitudes. Significant difference from corresponding controls is indicated (“**” p < 0.01; Mann–Whitney U test).

Parallel Induction of e-LTP/i-LTP and e-LTD/i-LTD Occurs at θ Frequency Stimulation

It is widely accepted that during the induction of e-LTP at SC-CA1 inputs by tetanic stimulation, inhibitory inputs undergo LTD (i-LTD, McMahon and Kauer, 1997 ; Chevaleyre and Castillo, 2003 ; Gibson et al., 2008 ), which is mediated by endocannabinoid signaling (Chevaleyre and Castillo, 2003 ). Conversely, repetitive presynaptic stimulation, even at relatively low frequency (10 Hz), induces endocannabinoid-mediated i-LTD that facilitates e-LTP induction at neighboring synapses (Chevaleyre and Castillo, 2004 ). Furthermore, the DSI procedure facilitates LTP induction known as endocannabinoid-mediated disinhibition, which requires 50-Hz tetanic stimulation with small numbers of pulses at excitatory synapses (Carlson et al., 2002 ). We, therefore, examined i-STDP induction following application of the θ frequency spike-timing protocol at excitatory synapses. We concurrently measured EPSCs and FF-IPSCs from the same CA1 pyramidal cells at spiking time intervals for both LTP (+4 to +6 ms) and –LTD (−24 to −20 ms). Stimuli of the same intensity were applied to the same SC inputs to evoke both EPSCs and FF-IPSCs while 40-ms voltage-steps from −80 to +10 mV (the EPSC reversal potential) were applied to the recorded neurons to monitor FF-IPSCs. This allowed the stable recording of both EPSCs and FF-IPSCs for more than 90 min (Figure 5 A). Surprisingly, we found that EPSCs and FF-IPSCs underwent parallel potentiation and depression of similar magnitudes following paired stimulation with spiking intervals that induced e-LTP (Figure 5 B) and e-LTD (Figure 5 C), although FF-IPSCs showed extensive short-term potentiation, regardless of the timing of the induction protocol. These results suggest that endocannabinoid-mediated i-LTD (Chevaleyre and Castillo, 2004 ) did not occur following the induction of e-LTP in our preparations (Figure 5 B). Moreover, we found that DSI preceding the LTD-timing protocol abolished only e-LTD, not i-LTD (Figure 5 D), demonstrating that the effects of the DSI were specific. Importantly, the magnitude of FF-IPSC immediately after the spike-timing protocol, regardless of the presence of DSI, was significantly greater than that of the basal FF-IPSC (indicated by red arrowheads, Figure 5 B–D), suggesting the occurrence of prominent postsynaptic GABA_AR-mediated inhibition during the spike-timing protocol at the θ frequency. This indicates that endocannabinoid-mediated disinhibition, i.e., passive modulation (Carlson et al., 2002 ), was also unlikely to have occurred. Thus, these results support the idea that postsynaptic GABA_AR-mediated inhibition actively controls the timing dependence of e-LTD at the θ frequency.

FIGURE 5

Figure 5. Parallel induction of e-LTP/i-LTP and e-LTD/i-LTD occurs under prominent postsynaptic GABA_AR-mediated inhibition. (A) Normalized EPSC/FF-IPSC amplitudes (%) without the spike-timing protocol. No changes in either EPSCs or FF-IPSCs were observed during the time course of the experiments. (B,C) EPSCs and FF-IPSCs were both potentiated at the LTP time intervals (B), and depressed at the –LTD time intervals (C). (D) DSI abolished LTD of EPSC, but not of FF-IPSC at the –LTD time intervals. Data represent normalized mean EPSCs/FF-IPSCs (±sem). Sample traces of EPSCs/FF-IPSCs (in B–D) before (1) and after (2) the induction protocols. Significant differences from corresponding controls are indicated (“*” p < 0.05 and “**” p < 0.01; Mann–Whitney U test).

Presynaptic GABA_BR-Mediated Inhibition Causes Frequency-Dependent e-LTD

Presynaptic glutamate release, which determines the EPSC amplitude, would be reduced if the frequency of presynaptic spiking were to increase (Ohliger-Frerking et al., 2003 ) as a result of activation of G-protein coupled receptors (GPCRs) at presynaptic terminals (Wu and Saggau, 1997 ). Because GABA_BRs are the major GPCRs at glutamatergic presynaptic terminals (Wu and Saggau, 1997 ), we tested whether presynaptic GABA_BR-mediated inhibition could also cause e-LTD as the frequency of the spike-timing protocol increased. Application of the LTP-timing protocol (time intervals at the +4 to +6 ms), which normally induced e-LTP at the θ frequency (Figure 6 A, top), had no significant effect on synaptic efficacy at 12 Hz (α frequency, for 6.7 s; 91.4 ± 10.6%, n = 6, in the Cs⁺-based internal solution; see Figure 6 D). A further increase in the frequency of paired stimulation to 25 Hz (β frequency) resulted in e-LTD, not e-LTP, in both K⁺- and Cs⁺-based solutions. Furthermore, the most robust e-LTP was observed (substantially greater than that induced by the LTP-timing protocol at 5 and 100 Hz) when the frequency of the LTP-timing protocol reached the γ frequency (50–80 Hz) in both the K⁺- and Cs⁺-based internal solutions. However, the optimal frequency for induction of the maximal e-LTP in the Cs⁺-based internal solution (50 Hz) appears to be lower than that in the K⁺-based internal solution (83 Hz). This may have resulted from the slower kinetics of EPSPs in the Cs⁺-based internal solution, which allows overlap with the preceding EPSPs to cause a postsynaptic depolarization, even at a lower frequency. It is noteworthy that during LTP induction at the γ frequency the postsynaptic GABA_AR function should be excitatory (Staley et al., 1995 ; Taira et al., 1997 ; Bracci et al., 2001 ), thereby likely enhancing the magnitude of e-LTP. These results support the idea that the induction of e-LTD also depends on the frequency of the spike-timing protocol, which is independent of postsynaptic K⁺ channel function.

FIGURE 6

Figure 6. Presynaptic GABA_BR-mediated inhibition causes frequency-dependent e-LTD at α/β frequencies. (A,D) Summary of frequency-dependent changes in synaptic efficacy induced by the spike-timing protocol for LTP-timing (+4 to +6 ms). Frequency-dependent effects (between 5 Hz and 100 Hz) in either the K⁺- or Cs⁺-based internal recording solution. Frequency-dependent e-LTD is induced at the same magnitude at 25 Hz and e-LTP is robust at the γ frequency in both recording solutions. (B) Bath application of CGP 35348 (1 mM, yellow bars) abolished e-LTD at 25 Hz, and instead induced e-LTP in both K⁺- and the Cs⁺-based internal recording solutions. (C) DSI failed to restore e-LTP induction at 25 Hz and had no significant effect on e-LTP at 5 Hz. Data represent normalized mean EPSCs (±sem). Membrane potentials during the spike-timing protocol (upper traces) and EPSCs (lower traces) before (1) and after (2) the induction protocols (in A–C). Scales: 100 pA (or 100 mV), 20 ms. Significant differences from corresponding controls are indicated (“**” p < 0.01; Mann–Whitney U test).

We examined further the effect of presynaptic GABA_BR-mediated inhibition on the frequency-dependent induction of e-LTD by applying the GABA_BR antagonist CGP35348 (1 mM, Davies and Collingridge, 1996) in the bath during the spike-timing protocol. This treatment abolished e-LTD induction at 25 Hz and resulted in a substantial e-LTP instead, in both K⁺- and Cs⁺-based solutions (Figures 6 B,D), suggesting that GABA_BR function is required for the induction of this frequency-dependent e-LTD. Interestingly, CGP35348 suppressed e-LTP induction at 5 Hz, the frequency at which presynaptic GABA_BR is most prominent at inhibitory inputs (Figure 6 D, Davies and Collingridge, 1993 ), suggesting that postsynaptic GABA_AR function is enhanced as a consequence of this treatment. Bath application of phaclofen, another GABA_BR antagonist, at the concentration (100 μM) that blocks presynaptic GABA_BR-mediated inhibition at excitatory (Hasselmo and Fehlau, 2001 ) but not at inhibitory (Davies and Collingridge, 1993 ) inputs during the spike-timing protocol, similarly converted the e-LTD to robust e-LTP at 25 Hz (178.0 ± 17.4%, n = 4). Thus, presynaptic GABA_BR-mediated inhibition at excitatory synapses is likely responsible for the frequency-dependent expression of e-LTD. Consistent with the idea that presynaptic GABA_BR-mediated inhibition, but not postsynaptic GABA_AR-mediated inhibition, may be a major inducer of frequency-dependent e-LTD, DSI affected e-LTD at 25 Hz only partially, while it had no significant effect on e-LTP induction at 5 Hz (Figure 6 C). This partial effect could be due to a reduction of GABA release by DSI (Lenz and Alger, 1999 ), which would also reduce presynaptic GABA_BR-mediated inhibition. Taken together, these results suggest that presynaptic GABA_BR-mediated inhibition controls the frequency dependence of e-LTD at α/β frequencies and accounts for the expression of e-LTP at θ and γ frequencies.

Discussion

We demonstrated that GABAergic interneuronal network activities control the spike timing- and frequency-dependent induction of the e-LTD (at –LTD and +LTD time intervals) that delineates the expression of e-LTP in hippocampal CA1 pyramidal cells (see Figure 7 A). The timing dependence of e-LTD induced by the θ frequency spike-timing protocol is regulated by both FF and FB postsynaptic GABA_AR-mediated inhibition. Feedback postsynaptic GABA_AR-mediated inhibition enforces the cooperative induction of e-LTP by causing e-LTD from a small population of the SC inputs during the LTP-timing. In contrast, the frequency-dependent expression of e-LTD at α/β frequencies is regulated predominantly by presynaptic GABA_BR-mediated inhibition. This e-LTD then segregates the expression of e-LTP into θ (timing-dependent) and γ (timing-independent) frequencies. The induction of similar magnitudes of e- and i-STDP in hippocampal SC-CA1 networks further demonstrates a novel mechanism by which GABAergic inhibition actively causes the induction of e-LTD.

FIGURE 7

Figure 7. Model for STDP controlled by GABA functions in the CA1 network. (A) Both spike timing- and frequency-dependence of e-STDP and differential GABA function that gates e-LTD. (B) Parallel induction of e-LTP/i-LTP and e-LTD/i-LTD. Upon application of the LTP- (left) or LTD-timing (right) protocol at 5 Hz, all excitatory synapses undergo, respectively, either e-LTP or e-LTD. e-LTP or e-LTD in the CB1R-expressing interneuron (both at inputs e2 and e3) propagates passively to input i-1, respectively, as either i-LTP or i-LTD.

Previously, the timing dependence of e-STDP was extensively studied using a low-frequency (<2 Hz) spike-timing induction protocol in various systems (Bi and Poo, 1998 ; Debanne et al., 1998 ; Zhang et al., 1998 ; Feldman, 2000 ; Froemke and Dan, 2002 ; Nevian and Sakmann, 2006 ). These studies led to the formulation of the asymmetric STDP learning rule: pre-before-post (causal order) results in e-LTP and post-before-pre (anti-causal order) results in e-LTD at the negative time intervals. The asymmetric STDP learning rule, which is compatible with Hebbian synaptic plasticity (Bliss and Collingridge, 1993 ) has been applied to explain bidirectional neuronal functions. In contrast, stimulation at the θ frequency (5 Hz), as in our previous (Nishiyama et al., 2000 ) and current studies and those of others (Wittenberg and Wang, 2006 ), results in the induction of a second e-LTD time window at positive time intervals. Also, a low-frequency (<2 Hz) spike-timing protocol induces a relatively broad e-LTD time window (>50 ms) at negative time intervals, whereas θ frequency induction of the STDP protocol induces relatively narrow e-LTD time windows (∼20 ms) at both –LTD and +LTD. Although the mechanisms of the appearance of narrow windows and an additional +LTD stimulated by the θ frequency are not understood, we found that while –LTD is fully sensitive to postsynaptic GABA_AR-mediated inhibition, +LTD is only partially sensitive (Wittenberg and Wang, 2006 ), suggesting the involvement of unidentified mechanisms other than postsynaptic GABA_AR-mediated inhibition and mechanisms related to spike afterhyperpolarization that suppress postsynaptic Ca²⁺ increase. Interestingly, computational analyses predict the stable presence of +LTD if the number of postsynaptic NMDARs increases (Shouval and Kalantzis, 2005 ). In support of this idea, recent electron microscopy study using the freeze-fracture replica method (Shinohara et al., 2008 ) demonstrated a much greater number of NMDARs at the spines of CA1 pyramidal cells compared to that reported previously (Racca et al., 2000 ). The greater number of NMDARs may be attributable to the persistent presence of +LTD induced by the θ frequency spike-timing protocol. Alternatively, it is also plausible that in addition to the different frequency dependency of FB IPSC recruitment in cortical neurons (Silberberg and Markram, 2007 ), cortical neurons may be innately endowed with an asymmetric, not a symmetric, STDP learning rule by these low-frequency stimuli.

In the current study, we applied DSI to investigate the timing dependence of e-STDP. Surprisingly, the DSI procedure caused drastic effects on the induction of e-STDP, even though a relatively minor population of interneurons (Glickfeld and Scanziani, 2006 ) are expected to be sensitive to it. Endocannabinoid receptor CB1R-expressing basket cells apparently receive inputs from both CA3 (the SC) and CA1 (the recurrent) pyramidal cells (Glickfeld and Scanziani, 2006 ). Therefore, these CB1R-expressing interneurons are highly likely to be involved in the determination of the timing dependence and the requirement for cooperative e-STDP by inducing both FF and FB postsynaptic GABA_AR-mediated inhibition to the CA1 pyramidal cells during the spike-timing protocol. It has been demonstrated that burst postsynaptic spiking is required for e-LTP induction by θ frequency stimuli at SC-CA1 synapses (Thomas et al., 1998 ; Pike et al., 1999 ; Meredith et al., 2003 ; Wittenberg and Wang, 2006 ). In this case, the burst spiking, which is known to silence A-type K⁺ channel activity in distal dendrites (Hoffman et al., 1997 ), likely facilitates the back-propagation of action potentials during the induction protocol. In our protocol, however, repetitive single (no burst) postsynaptic spiking is sufficient to induce e-LTP (Nishiyama et al., 2000 ), suggesting that the activated synapses are distant from functional A-type channels (Hoffman et al., 1997 ). We also found that the magnitudes of FF postsynaptic GABA_AR-mediated inhibition in thin slices (300–400 μm) were significantly smaller than in thick (500 μm) slices. Moreover, we observed FB postsynaptic GABA_AR-mediated inhibition during θ frequency postsynaptic spiking, which is unlikely to occur during the application of a low-frequency (<2 Hz) spike-timing protocol (Silberberg and Markram, 2007 ). Therefore, the molecular mechanisms underlying the induction of e-LTD through postsynaptic GABA_AR-mediated inhibition stimulated at the θ frequency likely differ from those of low-frequency stimulation-induced STDP (Bi and Poo, 1998 ; Feldman, 2000 ; Normann et al., 2000 ; Froemke and Dan, 2002 ). In support of this idea, the activity of mGluRs, rather than VDCCs, is required for e-LTD induction in our preparations (Nishiyama et al., 2000 ). We also compared the postsynaptic spike wave forms during the LTP-timing protocol in a Cs⁺-based internal solution with those in a K⁺-base internal solution and found a broadened spike width in the Cs⁺-based internal solution. However, it appears that postsynaptic spikes broadened by Cs⁺ do not contribute significantly to the features of e-STDP. This may also be consistent with the idea that Ca²⁺ entry through VDCCs is not essential for the induction of e-LTP (Bi and Poo, 1998 ) and e-LTD (Nishiyama et al., 2000 ). Our experimental paradigm is, thus, a unique procedure for the investigation of GABAergic activities during STDP. It has been shown that local dendritic spikes, without back-propagating action potentials, cause the induction of e-LTP (Golding et al., 2002 ). This type of synaptic plasticity is prominent at synaptic inputs of distal dendrites, where A-type channels prohibit invasion of single back-propagating action potentials (Hoffman et al., 1997 ). Our study, however, reveals the existence of bidirectional synaptic plasticity at proximal synaptic inputs that relies on the timing of single back-propagating action potentials. Depending on the location (i.e., proximal or distal dendrites) of activated synapses, different types of induction mechanisms, therefore, may be required to determine bidirectional synaptic plasticity.

Using weak SC input stimuli, we demonstrated that FB postsynaptic GABA_AR-mediated inhibition enforces the cooperative requirement for LTP induction (i.e., EPSC amplitude of >70 pA). However, we cannot exclude the following possibilities: The DSI procedure may diminish the postsynaptic GABA_AR-mediated actions by one half, including tonic inhibition (Mody and Pearce, 2004 ). Thus, the actual EPSC amplitude during the LTP-timing protocol (i.e., EPSP amplitude) may become greater than 70 pA as a result of the diminished shunting effect. Since, in our preparation, the EPSP amplitude is below 1 mV when the EPSC amplitude is smaller than 70 pA, it is not possible to accurately assess changes in EPSP amplitudes during the spike-timing protocol if they increased above the amplitude equivalent to EPSCs of 70 pA. We did not observe noticeable differences in the EPSP amplitudes in the presence of DSI compared to the control (data not shown). Moreover, the DSI permits induction of robust LTP, even at inputs with initial EPSC amplitudes of less than 40 pA (see Figure 4 B). Therefore, DSI is unlikely to have caused increases of the actual EPSP during the spike-timing protocol. We demonstrated the absence of functional FF-IPSCs when the EPSC amplitude was less than 70 pA with the use of single stimuli at SC inputs. However, in response to sequential stimuli, such as during the spike-timing protocol, FF-IPSCs may become manifest due to sequential pulse facilitation, particularly in interneurons that receive both SC and recurrent inputs. Even if a potential contamination from FF-IPSCs occurs, it is likely negligible compared to the prominent FB-IPSCs (an IPSP amplitude of ca. −34 mV, which is equivalent to an IPSC amplitude of ca. 800 to 900 pA). Therefore, our conclusion that FB postsynaptic GABA_AR-mediated inhibition enforces the cooperative requirement for LTP induction would not be affected significantly.

We showed that FF-IPSCs undergo i-LTP and i-LTD, which are associated, respectively, with e-LTP and e-LTD induced by the θ frequency spike-timing protocol at SC-CA1 excitatory synapses. Two distinct mechanisms, passive and active induction, that cause synaptic efficacy changes in excitatory inputs to interneurons have been demonstrated. Both e-LTP and e-LTD propagate passively to FB interneurons (Maccaferri and McBain, 1995 , 1996 ). Interneurons also express e-LTP directly (Kullmann and Lamsa, 2007 ) at the SC input (Lamsa et al., 2005 ) and at the input from CA1 pyramidal cells (Lamsa et al., 2007 ). Interestingly, e-LTP at the SC-FF interneuron synapses propagates to the inhibitory input to CA1 pyramidal cells (Lamsa et al., 2005 ). The parallel induction of e-LTP/i-LTP and e-LTD/i-LTD that we observed may, therefore, depend on both the passive and active mechanisms of induction of inhibitory synaptic plasticity (Figure 7 B). Since CB1R-expressing basket cells (Glickfeld and Scanziani, 2006 ) receive inputs from both CA3 and from the recorded CA1 pyramidal cells, the magnitudes of which are each sufficient to spike these interneurons, both e-LTP and e-LTD can be induced. These excitatory inputs to interneurons may follow a similar STDP learning rule for excitatory inputs to recorded CA1 pyramidal cells. During the LTP-timing protocol, the disynaptic inputs from CA3 and CA1 should be the LTP-timing for one another (i.e., time intervals of ∼0 ms). Conversely, during the LTD-timing protocol, these inputs become either the +LTD or –LTD time intervals for one another, because the time difference between two inputs to an interneuron is approximately 20 ms, which is the LTD time interval. Therefore, upon application of the spike-timing protocol, excitatory inputs to both CA1 recording cells and interneurons undergo e-STDP with the same polarity. Then, either e-LTP or e-LTD in interneurons propagates passively to inputs to CA1 recording cells to become, respectively, either i-LTP or i-LTD. Thus, the parallel induction of e- and i-LTP may help to maintain the temporal resolution of excitatory synaptic integration and the generation of an action potential in excitatory LTP-expressing CA1 pyramidal cells (Pouille and Scanziani, 2001 ; Lamsa et al., 2005 ).

We also showed that the frequency dependence of e-LTD at α/β frequencies, which likely delineates the expression of e-LTP at the θ and γ frequencies, requires presynaptic GABA_BR-mediated inhibition. This is consistent with the optimal frequencies required for induction of presynaptic GABA_BR-mediated inhibition, which are maximal at the θ and above the α/β frequencies for inhibitory and excitatory presynaptic terminals, respectively (Davies and Collingridge, 1996 ; Ohliger-Frerking et al., 2003 ). In addition to the appearance of e-LTD at α/β frequencies, we observed that the magnitude of e-LTP begins to decrease if the frequency of the spike-timing protocol increases above the γ frequency (i.e., at 100 Hz). Although the molecular mechanisms underlying the reduction of e-LTP at a high frequency requires further investigation, its non-linearity apparently violates Ca²⁺ theory for synaptic plasticity. Despite this, our study demonstrates that e-STDP in CA1 pyramidal cells is likely self-limited by the neural network function. It has been reported that postsynaptic GABA_BRs inactivate VDCCs independent of GIRK activity (Perez-Garci et al., 2006 ). However, since e-LTD at the θ frequency appears to be independent of VDCC activity (Nishiyama et al., 2000 ), postsynaptic GABA_BRs may not be involved in e-LTD induction. The potential contribution of postsynaptic GABA_BR-mediated inhibition to frequency-dependent e-LTD at the α/β frequencies requires further investigation.

Taken together, our study demonstrates the mechanism by which GABAergic inhibitory activities cause e-LTD at time intervals of ±20 ms (at the θ frequency) or at α/β frequencies to control the timing- and frequency-dependent features of e-STDP; e-LTP/e-LTD switches within the γ cycle (Nishiyama et al., 2000 ) and e-LTP appears at the θ and γ frequencies. Therefore, the GABAergic interneuronal network activities that regulate STDP may similarly govern hippocampal oscillations in vivo (Buzsáki, 2002 ; Csicsvari et al., 2003 ; Lisman et al., 2005 ). Interestingly, the parallel induction of STDP demonstrated in this study, in which e-LTP likely depolarizes dendrites when i-LTP hyperpolarizes the soma, is consistent with the in vivo hippocampal θ oscillation that yields a phase-shift, a current sink at the dendrite and a current source at the soma resulting from GABAergic and cholinergic activities (Kamondi et al., 1998 ; Buzsáki, 2002 ). Future studies will determine whether the underlying mechanisms of GABAergic interneuronal network activities that govern STDP also apply to θ and γ oscillations.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This work was supported by the CRCNS program NSF/NIH (MH068027), the Whitehall Foundation Inc. and the Brain Science Foundation. We thank Drs. M. M. Poo, D. Debanne, H. Eichenbaum, B. H. Gaehwiler, J. E. Lisman, N. Spruston and H. Sei for discussion and comments; W. Jelinek and N. Cowen for critical comments on the manuscript; K. Kato for technical support.

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