Original Research ARTICLE

Front. Plant Sci., 19 June 2013 | doi: 10.3389/fpls.2013.00181

Comparative feedstock analysis in Setaria viridis L. as a model for C4 bioenergy grasses and panicoid crop species

Carloalberto Petti1, Andrew Shearer1, Mizuki Tateno1, Matthew Ruwaya2, Sue Nokes2, Tom Brutnell3 and Seth DeBolt1*
  • 1Plant Physiology, Department of Horticulture, College of Agriculture, Food and the Environment, University of Kentucky, Lexington, KY, USA
  • 2Department of Biosystems and Agricultural Engineering, University of Kentucky, Lexington, KY, USA
  • 3Enterprise Institute for Renewable Fuels, Donald Danforth Plant Science Center, St. Louis, MO, USA

Second generation feedstocks for bioethanol will likely include a sizable proportion of perennial C4 grasses, principally in the Panicoideae clade. The Panicoideae contain agronomically important annual grasses including Zea mays L. (maize), Sorghum bicolor (L.) Moench (sorghum), and Saccharum officinarum L. (sugar cane) as well as promising second generation perennial feedstocks including Miscanthus × giganteus and Panicum virgatum L. (switchgrass). The underlying complexity of these polyploid grass genomes is a major limitation for their direct manipulation and thus driving a need for rapidly cycling comparative model. Setaria viridis (green millet) is a rapid cycling C4 panicoid grass with a relatively small and sequenced diploid genome and abundant seed production. Stable, transient, and protoplast transformation technologies have also been developed for Setaria viridis making it a potentially excellent model for other C4 bioenergy grasses. Here, the lignocellulosic feedstock composition, cellulose biosynthesis inhibitor response and saccharification dynamics of Setaria viridis are compared with the annual sorghum and maize and the perennial switchgrass bioenergy crops as a baseline study into the applicability for translational research. A genome-wide systematic investigation of the cellulose synthase-A genes was performed identifying eight candidate sequences. Two developmental stages; (a) metabolically active young tissue and (b) metabolically plateaued (mature) material are examined to compare biomass performance metrics.

Keywords: Setaria, cell wall, lignocellulose, Panicoideae, biofuel, cellulose synthase

Citation: Petti C, Shearer A, Tateno M, Ruwaya M, Nokes S, Brutnell T and DeBolt S (2013) Comparative feedstock analysis in Setaria viridis L. as a model for C4 bioenergy grasses and panicoid crop species. Front. Plant Sci. 4:181. doi: 10.3389/fpls.2013.00181

Received: 29 March 2013; Accepted: 20 May 2013;
Published online: 19 June 2013.

Edited by:

Samuel P. Hazen, University of Massachusetts, USA

Reviewed by:

Markus Pauly, University of California at Berkeley, USA
Christian Voigt, University of Hamburg, Germany

Copyright: © 2013 Petti, Shearer, Tateno, Ruwaya, Nokes, Brutnell and DeBolt. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

*Correspondence: Seth DeBolt, Plant Physiology, Department of Horticulture, College of Agriculture, Food and the Environment, University of Kentucky, N318 Agricultural Science Center North, Lexington, KY 40546, USA e-mail: sdebo2@uky.edu

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