%A Hey,Ying-Ying %A Tan,Jonathan K. H. %A O’Neill,Helen C. %D 2016 %J Frontiers in Immunology %C %F %G English %K Dendritic Cells,Monocytes,Spleen,Myeloid Cells,development %Q %R 10.3389/fimmu.2015.00652 %W %L %M %P %7 %8 2016-January-11 %9 Original Research %+ Prof Helen C. O’Neill,Faculty of Health Sciences and Medicine, Bond University,Australia,honeill@bond.edu.au.old %# %! Myeloid cells in spleen %* %< %T Redefining Myeloid Cell Subsets in Murine Spleen %U https://www.frontiersin.org/articles/10.3389/fimmu.2015.00652 %V 6 %0 JOURNAL ARTICLE %@ 1664-3224 %X Spleen is known to contain multiple dendritic and myeloid cell subsets, distinguishable on the basis of phenotype, function and anatomical location. As a result of recent intensive flow cytometric analyses, splenic dendritic cell (DC) subsets are now better characterized than other myeloid subsets. In order to identify and fully characterize a novel splenic subset termed “L-DC” in relation to other myeloid cells, it was necessary to investigate myeloid subsets in more detail. In terms of cell surface phenotype, L-DC were initially characterized as a CD11bhiCD11cloMHCIILy6CLy6G subset in murine spleen. Their expression of CD43, lack of MHCII, and a low level of CD11c was shown to best differentiate L-DC by phenotype from conventional DC subsets. A complete analysis of all subsets in spleen led to the classification of CD11bhiCD11cloMHCIILy6CloLy6G cells as monocytes expressing CX3CR1, CD43 and CD115. Siglec-F expression was used to identify a specific eosinophil population, distinguishable from both Ly6Clo and Ly6Chi monocytes, and other DC subsets. L-DC were characterized as a clear subset of CD11bhiCD11cloMHCIILy6CLy6G cells, which are CD43+, Siglec-F and CD115. Changes in the prevalence of L-DC compared to other subsets in spleens of mutant mice confirmed the phenotypic distinction between L-DC, cDC and monocyte subsets. L-DC development in vivo was shown to occur independently of the BATF3 transcription factor that regulates cDC development, and also independently of the FLT3L and GM-CSF growth factors which drive cDC and monocyte development, so distinguishing L-DC from these commonly defined cell types.