This article is part of the Research Topic Pathogenesis of tuberculosis and paratuberculosis

Original Research ARTICLE

Front. Microbiol., 11 January 2012 | doi: 10.3389/fmicb.2011.00281

Inhibition of the plasma-membrane-associated serine protease cathepsin G by Mycobacterium tuberculosis Rv3364c suppresses caspase-1 and pyroptosis in macrophages

Lia Danelishvili1, Jamie L. Everman1,2, Michael J. McNamara1,3 and Luiz E. Bermudez1,2,3*
  • 1 Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA
  • 2 Department of Microbiology, College of Science, Oregon State University, Corvallis, OR, USA
  • 3 Program of Molecular and Cell Biology, Oregon State University, Corvallis, OR, USA

Tuberculosis is a disease associated with the infection of a great part of the world’s population and is responsible for the death of two to three million people annually. Mycobacterium tuberculosis infects macrophages and subverts its mechanisms of killing. The pathogen suppresses macrophage apoptosis by many different mechanisms. We describe that, upon uptake by macrophages, M. tuberculosis overexpresses an operon Rv3361c-Rv3365c and secretes Rv3364c. The Rv3365c knockout strain is deficient in apoptosis inhibition. The Rv3364c protein binds to the serine protease cathepsin G on the membrane, inhibiting its enzymatic activity and the downstream activation of caspase-1-dependent apoptosis. In summary, M. tuberculosis prevents macrophage pyroptosis by a novel mechanism involving cytoplasmic surveillance proteins.

Keywords: Mycobacterium tuberculosis, macrophages, apoptosis, pyroptosis, inhibition, caspase-1-dependent

Citation: Danelishvili L, Everman JL, McNamara MJ and Bermudez LE (2012) Inhibition of the plasma-membrane-associated serine protease cathepsin G by Mycobacterium tuberculosis Rv3364c suppresses caspase-1 and pyroptosis in macrophages. Front. Microbio. 2:281. doi: 10.3389/fmicb.2011.00281

Received: 19 September 2011; Accepted: 28 December 2011;
Published online: 11 January 2012.

Edited by:

Adel M. Talaat, University of Wisconsin Madison, USA

Reviewed by:

Jordi Torrelles, Ohio State University, USA
Steve Blanke, University of Illinois Urbana-Champaign, USA

Copyright: © 2012 Danelishvili, Everman, McNamara and Bermudez. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

*Correspondence: Luiz E. Bermudez, Biomedical Sciences, College of Veterinary Medicine, 105 Magruder Hall, Oregon State University, Corvallis, OR 97331, USA. e-mail: luiz.bermudez@oregonstate.edu

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