Original Research ARTICLE
Phototrophic biofilm assembly in microbial-mat-derived unicyanobacterial consortia: model systems for the study of autotroph-heterotroph interactions
- 1Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA
- 2Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA, USA
- 3Scientific Resources Division, William R. Wiley Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA
Microbial autotroph-heterotroph interactions influence biogeochemical cycles on a global scale, but the diversity and complexity of natural systems and their intractability to in situ manipulation make it challenging to elucidate the principles governing these interactions. The study of assembling phototrophic biofilm communities provides a robust means to identify such interactions and evaluate their contributions to the recruitment and maintenance of phylogenetic and functional diversity over time. To examine primary succession in phototrophic communities, we isolated two unicyanobacterial consortia from the microbial mat in Hot Lake, Washington, characterizing the membership and metabolic function of each consortium. We then analyzed the spatial structures and quantified the community compositions of their assembling biofilms. The consortia retained the same suite of heterotrophic species, identified as abundant members of the mat and assigned to Alphaproteobacteria, Gammaproteobacteria, and Bacteroidetes. Autotroph growth rates dominated early in assembly, yielding to increasing heterotroph growth rates late in succession. The two consortia exhibited similar assembly patterns, with increasing relative abundances of members from Bacteroidetes and Alphaproteobacteria concurrent with decreasing relative abundances of those from Gammaproteobacteria. Despite these similarities at higher taxonomic levels, the relative abundances of individual heterotrophic species were substantially different in the developing consortial biofilms. This suggests that, although similar niches are created by the cyanobacterial metabolisms, the resulting webs of autotroph-heterotroph and heterotroph-heterotroph interactions are specific to each primary producer. The relative simplicity and tractability of the Hot Lake unicyanobacterial consortia make them useful model systems for deciphering interspecies interactions and assembly principles relevant to natural microbial communities.
Keywords: phototrophic biofilm model system, microbial diversity, primary succession, real-time PCR, community metabolomics, confocal microscopy, image analysis, microcosm
Citation: Cole JK, Hutchison JR, Renslow RS, Kim Y-M, Chrisler WB, Engelmann HE, Dohnalkova AC, Hu D, Metz TO, Fredrickson JK and Lindemann SR (2014) Phototrophic biofilm assembly in microbial-mat-derived unicyanobacterial consortia: model systems for the study of autotroph-heterotroph interactions. Front. Microbiol. 5:109. doi: 10.3389/fmicb.2014.00109
Received: 16 November 2013; Accepted: 04 March 2014;
Published online: 07 April 2014.
Edited by:Kelly Wrighton, The Ohio State University, USA
Reviewed by:Ross Carlson, Montana State University, USA
Jean-Baptiste Ramond, University of Pretoria, South Africa
Copyright © 2014 Cole, Hutchison, Renslow, Kim, Chrisler, Engelmann, Dohnalkova, Hu, Metz, Fredrickson and Lindemann. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Stephen R. Lindemann, Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, 902 Battelle Boulevard, MSIN: J4-18, Richland, WA 99352, USA e-mail: firstname.lastname@example.org
†These authors have contributed equally to this work and are listed alphabetically.