%A Pepe-Ranney,Charles
%A Campbell,Ashley N.
%A Koechli,Chantal N.
%A Berthrong,Sean
%A Buckley,Daniel H.
%D 2016
%J Frontiers in Microbiology
%C
%F
%G English
%K stable isotope probing,Carbon Cycle,decomposition,Verrucomicrobia,Cellulose,Soil,trophic,DNA-SIP
%Q
%R 10.3389/fmicb.2016.00703
%W
%L
%M
%P
%7
%8 2016-May-12
%9 Original Research
%+ Dr Daniel H. Buckley,School of Integrative Plant Sciences, Cornell University,Ithaca, NY, USA,dbuckley@cornell.edu
%#
%! High resolution stable isotope probing
%*
%<
%T Unearthing the Ecology of Soil Microorganisms Using a High Resolution DNA-SIP Approach to Explore Cellulose and Xylose Metabolism in Soil
%U https://www.frontiersin.org/articles/10.3389/fmicb.2016.00703
%V 7
%0 JOURNAL ARTICLE
%@ 1664-302X
%X We explored microbial contributions to decomposition using a sophisticated approach to DNA Stable Isotope Probing (SIP). Our experiment evaluated the dynamics and ecological characteristics of functionally defined microbial groups that metabolize labile and structural C in soils. We added to soil a complex amendment representing plant derived organic matter substituted with either 13C-xylose or 13C-cellulose to represent labile and structural C pools derived from abundant components of plant biomass. We found evidence for 13C-incorporation into DNA from 13C-xylose and 13C-cellulose in 49 and 63 operational taxonomic units (OTUs), respectively. The types of microorganisms that assimilated 13C in the 13C-xylose treatment changed over time being predominantly Firmicutes at day 1 followed by Bacteroidetes at day 3 and then Actinobacteria at day 7. These 13C-labeling dynamics suggest labile C traveled through different trophic levels. In contrast, microorganisms generally metabolized cellulose-C after 14 days and did not change to the same extent in phylogenetic composition over time. Microorganisms that metabolized cellulose-C belonged to poorly characterized but cosmopolitan soil lineages including Verrucomicrobia, Chloroflexi, and Planctomycetes.