%A Barnes,Christopher J. %A Maldonado,Carla %A Frøslev,Tobias G. %A Antonelli,Alexandre %A Rønsted,Nina %D 2016 %J Frontiers in Microbiology %C %F %G English %K root-associated fungi,soil fungi,Cinchona calisaya,fungal spatial-scaling,beta-diversity %Q %R 10.3389/fmicb.2016.01377 %W %L %M %P %7 %8 2016-August-31 %9 Original Research %+ Christopher J. Barnes,Nina Rønsted Lab, Natural History Museum of Denmark, University of Copenhagen,Copenhagen, Denmark,drcjbarnes@gmail.com %# %! Beta-diversity within the Bolivian Andes %* %< %T Unexpectedly High Beta-Diversity of Root-Associated Fungal Communities in the Bolivian Andes %U https://www.frontiersin.org/articles/10.3389/fmicb.2016.01377 %V 7 %0 JOURNAL ARTICLE %@ 1664-302X %X Bolivia is one of the most biologically diverse countries on the planet. Between the Andes and the Amazon drainage basin spans the Yungas, a vast forested region shown to be extremely species rich in macro-organisms. However, it remains unclear whether this high diversity is also reflected in microbial diversity. Here we assess the genetic, taxonomic and functional diversity of root-associated fungi surrounding Cinchona calisaya trees, a typical element of the intermediate altitudes of the Bolivian Yungas. We determine the relative effects of edaphic properties, climate, and geography in regulating fungal community assembly. We show that α-diversity for these fungal communities was similar to temperate and arid ecosystems, averaging 90.1 operational taxonomic units (OTUs) per sample, with reads predominantly assigned to the Ascomycota phylum and with a saprotrophic lifestyle. ß-diversity was calculated as the distance-decay rate, and in contrast to α-diversity, was exceptionally high with a rate of −0.407. Soil properties (pH and P) principally regulated fungal community assembly in an analogous manner to temperate environments, with pH and phosphorus explaining 7.8 and 7.2% of community variation respectively. Surprisingly, altitude does not influence community formation, and there is limited evidence that climate (precipitation and temperature) play a role. Our results suggest that sampling should be performed over a wide geographical and environmental range in order to capture the full root-associated fungal diversity in subtropical regions. This study sheds further light on the diversity and distribution of the world's “hidden biodiversity.”