Skip to main content

PERSPECTIVE article

Front. Microbiol., 23 September 2016
Sec. Microbiological Chemistry and Geomicrobiology
This article is part of the Research Topic Origin and Evolution of Photosynthesis View all 7 articles

Cyanobacterial Diazotrophy and Earth’s Delayed Oxygenation

  • 1Department of Earth Sciences, University of California, Riverside, Riverside, CA, USA
  • 2School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atlanta, GA, USA

The redox landscape of Earth’s ocean-atmosphere system has changed dramatically throughout Earth history. Although Earth’s protracted oxygenation is undoubtedly the consequence of cyanobacterial oxygenic photosynthesis, the relationship between biological O2 production and Earth’s redox evolution remains poorly understood. Existing models for Earth’s oxygenation cannot adequately explain the nearly 2.5 billion years delay between the origin of oxygenic photosynthesis and the oxygenation of the deep ocean, in large part owing to major deficiencies in our understanding of the coevolution of O2 and Earth’s key biogeochemical cycles (e.g., the N cycle). For example, although possible links between O2 and N scarcity have been previously explored, the consequences of N2 limitation for net biological O2 production have not been examined thoroughly. Here, we revisit the prevailing view that N2 fixation has always been able to keep pace with P supply and discuss the possibility that bioavailable N, rather than P, limited export production for extended periods of Earth’s history. Based on the observation that diazotrophy occurs at the expense of oxygenesis in the modern ocean, we suggest that an N-limited biosphere may be inherently less oxygenic than a P-limited biosphere—and that cyanobacterial diazotrophy was a primary control on the timing and tempo of Earth’s oxygenation by modulating net biogenic O2 fluxes. We further hypothesize that negative feedbacks inhibit the transition between N and P limitation, with the implication that the pervasive accumulation of O2 in Earth’s ocean-atmosphere system may not have been an inevitable consequence of oxygenic photosynthesis by marine cyanobacteria.

Introduction

Cyanobacteria are responsible for two major metabolic innovations: (1) oxygenic photosynthesis and (2) aerobic N2 fixation. The former initiated the protracted oxygenation of Earth’s ocean-atmosphere system and eventually set the stage for the evolution of complex animal life (reviewed by Lyons et al., 2014). The latter is also a remarkable achievement considering that nitrogenase, the enzyme responsible for cleaving the dinitrogen triple bond, is irreversibly inactivated by O2 (reviewed by Berman-Frank et al., 2003). Despite the apparent incompatibility of oxygenesis and diazotrophy, cyanobacteria dominate N2 fixation in the modern well-oxygenated ocean (Capone et al., 1997). This uniquely cyanobacterial ability to reconcile both environmental (exogenous) and photosynthetic (endogenous) O2 with N2 fixation (diazotrophy) acts to minimize the potential for biospheric N limitation and favors primary production that is broadly limited by the bioavailability of P in the modern ocean (Redfield, 1958; Tyrrell, 1999). Nevertheless, the details of the co-evolution of cyanobacterial innovation, the O2 content of the ocean-atmosphere system, and Earth’s nutrient cycles during the Precambrian remain unclear. In particular, the origin of oxygenic photosynthesis and the oxygenation of Earth’s atmosphere appear to be temporally decoupled, and there are major uncertainties regarding the primary controls on the timing and tempo of Earth’s oxygenation (Lyons et al., 2014). The dynamics of the transition from a broadly anaerobic biosphere supported by NH4+ and anaerobic diazotrophy to an aerobic biosphere dominated by NO3- assimilation and O2-tolerant cyanobacterial diazotrophy are also poorly known (Fennel et al., 2005), muddling our understanding of the causal relationships at play between O2 production, oxygenation, and marine nutrient cycling.

Herein, we examine the metabolic capabilities of extant diazotrophic cyanobacteria in the context of recent constraints on the oceanographic conditions of the mid-Proterozoic (~1.8 to 0.8 billion years ago or Ga). We discuss the possibility that the spatiotemporal redox landscape of the mid-Proterozoic ocean would have been less than ideal for cyanobacterial N2 fixation, favoring widespread N scarcity rather than P limitation. We also explore the implications of N limitation for biogenic O2 fluxes from the marine biosphere, hypothesizing that cyanobacterial production would have been less oxygenic under the O2 and nutrient conditions that typified the mid-Proterozoic ocean—potentially providing a biological mechanism for stabilizing pO2 at values much lower than those of the modern Earth.

Earth’s Redox Evolution

The origin of cyanobacterial oxygenic photosynthesis significantly predates the permanent and pervasive oxygenation of Earth’s ocean-atmosphere system (Lyons et al., 2014). A growing collection of trace metal and isotopic records collectively indicates the accumulation of dissolved O2 in highly productive regions of the shallow ocean up to 50–500 million years before the initial oxygenation of the atmosphere ~2.4 Ga (e.g., Anbar et al., 2007; Kendall et al., 2010; Planavsky et al., 2014a)—a conclusion that is supported by recent biogeochemical models (Olson et al., 2013; Reinhard et al., 2013a). Evidence for dissolved O2 prior to the initial oxygenation of Earth’s atmosphere in the early Paleoproterozoic includes N isotope records that capture the onset of coupled nitrification and denitrification, implying metabolic utilization of O2 in the oxidation of NH4+ to N oxyanions in the surface ocean during the late Archean (Garvin et al., 2009; Godfrey and Falkowski, 2009). It is likely that dissolved O2 also accumulated, at least locally, in freshwater environments and within microbial mats, in isolation from prevailing oceanic and atmospheric conditions (Herman and Kump, 2005; Lalonde and Konhauser, 2015; Sumner et al., 2015). As such, inhibition of nitrogenase by O2 may have been an issue in both terrestrial and marine oxygen oases well before the first rise of atmospheric oxygen, and an initial inability to fix N2 aerobically to replenish bioavailable N lost through denitrification may have limited cyanobacterial proliferation in the late Archean (Scott et al., 2011).

It is also clear that Earth’s protracted oxygenation was not unidirectional and featured dramatic variability on a variety of timescales in addition to spatial heterogeneity (Lyons et al., 2014). Although relatively high atmospheric pO2 has been suggested during the Paleoproterozoic Lomagundi Event ~2.3–2.1 Ga (Bekker and Holland, 2012; Rybacki et al., 2016), several geochemical records collectively suggest that environmental O2 levels subsequently fell precipitously at the onset of the ensuing Boring Billion (~1.8–0.8 Ga) and remained relatively low until the late Neoproterozoic (Planavsky et al., 2012; Partin et al., 2013). Recent O2 constraints suggest mid-Proterozoic pO2 of less than 10-3 times the present atmospheric level as a long-term average (Planavsky et al., 2014b), with the implication that the dissolved O2 landscape of the mid-Proterozoic ocean was not markedly different than that of the late Archean (Reinhard et al., 2016). The emerging view is that the surface ocean was likely characterized by low-micromolar O2 that was highly variable in space and time (Reinhard et al., 2016), while the deep ocean remained anoxic (Poulton and Canfield, 2011)—for the vast majority of Earth history. Indeed, despite evidence for oxygenation in the late Neoproterozoic (Sahoo et al., 2012; Planavsky et al., 2014b), clear evidence for pervasive and permanent oxygenation within the ocean is lacking until the Phanerozoic (Kendall et al., 2015; Sperling et al., 2015). In this view, the so-called Great Oxidation Event of the early Paleoproterozoic has relatively minor ecological implications compared to subsequent oxygenation in the late Neoproterozoic and early Phanerozoic. Meanwhile, it remains unclear why biospheric O2 production failed to result in stably oxygenated surface environments and deep ocean oxygenation for ~2.5 billion years after cyanobacterial emergence.

Oxygenation and Denitrification

The persistence of O2 stratification, that is, the widely held view that O2 was mostly restricted to the shallow ocean until the Paleozoic, has profound implications for the operation of the N cycle during Precambrian time. Denitrification, the reduction of bioavailable NO3- to N2 via anaerobic respiration of organic matter, results in a substantial loss of bioavailable N in O2-deficient environments. Anaerobic oxidation of NH4+ coupled to NO2- reduction (anammox) also represents a leak for bioavailable N as inert N2 (Kuypers et al., 2005). In the modern well-oxygenated ocean, denitrification and anammox are limited to organic-rich sediments and oxygen minimum zones (OMZs) underlying high-nutrient waters where the subsurface demand for respiratory electron acceptors locally exceeds O2 supply (Gruber and Sarmiento, 1997). Denitrification and anammox would also have been limited prior to the origin of oxygenic photosynthesis as the result of limited NO2- and NO3- synthesis in an anoxic ocean. The redox-stratified water column of the Proterozoic Earth, however, would have been particularly conducive to widespread loss of bioavailable N, because surface oxygenation would have allowed oxidation of NH4+ to N oxyanions by O2 in surface environments to support continued subsurface N2 synthesis via denitrification and anammox (Fennel et al., 2005; Garvin et al., 2009; Godfrey and Falkowski, 2009).

Oxygenation and N2 Fixation

The likelihood of extensive loss of fixed N through denitrification requires a correspondingly large influx of bioavailable N via diazotrophy at steady-state; if N2 fixation cannot keep pace with denitrification, export production must decline until denitrification and N2 fixation are balanced (e.g., Deutsch et al., 2007). Thus, widespread denitrification may potentially modulate the long-term oxidation dynamics of Earth’s ocean-atmosphere system by limiting the burial of reduced carbon and sulfur if N2 fixation was less efficient than today. Indeed, there are several reasons that the Proterozoic surface ocean may have been less than ideal for cyanobacterial diazotrophy. Based on the metabolic capabilities of extant cyanobacteria, we suggest that the low-level, spatially and temporally variable O2 concentrations that likely characterized the Proterozoic surface ocean would have posed a worst-case scenario for N2 fixation by cyanobacteria—thus allowing for the possibility of an N-limited biosphere for much of Earth history.

Extant diazotrophic cyanobacteria differ dramatically in their ability to cope with environmental O2, and many N2 fixing cyanobacteria are actually obligate aerobes despite nitrogenase sensitivity to O2 (reviewed by Fay, 1992; Gallon, 1992). For these cyanobacteria, aerobic respiration provides: (1) a strategy for protecting nitrogenase by contributing to the maintenance of low intracellular O2 and (2) an energy source to support the demanding task of cleaving the dinitrogen triple bond while simultaneously maintaining O2 defense mechanisms. For example, despite the need for anoxic conditions within heterocysts, these differentiated cells are metabolically costly, and the rate at which O2 can diffuse into heterocysts can be an important control on the rate of N2 fixation in low O2 environments (Stal, 2009). Among modern heterocystous cyanobacteria, the metabolic machinery for N2 fixation appears to be optimized for present day air-saturated or even modestly elevated O2 concentrations (e.g., ~300 μM for Anabaena cylindrica; see Tomitani et al., 2006). Ambient O2 concentrations also appear to control both the rate and frequency of heterocyst growth (Kangatharalingam et al., 1992), with heterocysts grown under O2-replete conditions demonstrating lower sensitivity to environmental instability (e.g., diurnal fluctuations in O2 availability; Rippka and Stanier, 1978). In sum, the ability to cope with inhibitory levels of O2 via heterocystic cellular differentiation seems, rather ironically, to be favored by persistently elevated O2. These observations highlight the possibility that heterocystous diazotrophy may have been challenging for marine cyanobacteria for the vast majority of Earth history. Indeed, unambiguous marine heterocyst fossils are absent until the Phanerozoic (Golubic et al., 1995), and heterocystous cyanobacteria are relatively uncommon in well-oxygenated environments of the modern pelagic ocean (Zehr, 2011).

The non-heterocystous cyanobacteria that are capable of aerobic diazotrophy are generally tolerant of lower O2 concentrations than the heterocystous cyanobacteria. Nonetheless, some non-heterocystous cyanobacteria are also obligate aerobes, and low O2 availability may also reduce the rate of non-heterocystous N2 fixation (reviewed by Fay, 1992). Most aerobic non-heterocystous diazotrophic cyanobacteria optimally fix N2 at dissolved O2 concentrations on the order of ~10–50% of present marine levels (see Tomitani et al., 2006). Thus, it is likely that Proterozoic shallow marine dissolved O2 concentrations (~1% of modern; Reinhard et al., 2016) would have been suboptimal for diazotrophic cyanobacteria that were obligate aerobes. Meanwhile, even ~1% of modern dissolved O2 is sufficient to completely inhibit anaerobic N2 fixation among the majority of microaerobic non-heterocystous cyanobacteria (Bergman et al., 1997) and the obligate anaerobes capable of diazotrophy (e.g., some methanogens; Murray and Zinder, 1984).

N Scarcity and Net O2 Production

Not all O2-tolerant, N2-fixing cyanobacteria meet the energetic demands of N2 fixation through aerobic respiration. Although O2 is not typically limiting in the open ocean today, one of the most successful marine diazotrophs, Trichodesmium, does not depend on exogenous O2 to respire stored carbohydrates. Instead, Trichodesmium is able to use its photosynthetic enzymes to power N2 fixation (reviewed by Bergman et al., 2013). However, because Trichodesmium performs diazotrophy at the expense of photosynthesis during daylight, midday O2 production is markedly reduced and potentially reversed when bioavailable N is limiting (Berman-Frank et al., 2001). Several other widespread unicellular marine cyanobacteria also fix N2 during the day using electron transfer from photosystem I (PSI)—and these cyanobacteria completely lack the ability to perform oxygenic photosynthesis (Zehr et al., 2008; Bothe et al., 2010; Tripp et al., 2010). These alternative strategies for coping with N scarcity would have been particularly advantageous if the ancient marine O2 landscape was not persistently favorable for either obligately aerobic or anaerobic N2 fixation. If PSI-dependent N2 fixation by O2 tolerant diazotrophic cyanobacteria was more common during Proterozoic time, cyanobacterial production may have been widely anoxygenic for much of Earth history. It follows that even if previous claims that the C isotope record precludes major changes in the burial of reduced organic carbon are correct (e.g., Holland, 2002), the C isotope record does not rule out major changes in net O2 fluxes from the marine biosphere through time.

This potential for reduced net oxygenesis in a low-O2 ocean is potentially exacerbated by the enhanced ecological significance of anoxygenic phototrophy coupled to the oxidation of H2S (Johnston et al., 2009; Hamilton et al., 2016). Indeed, biomarkers and several inorganic proxy records point to local subsurface H2S accumulation (euxinia) during the Proterozoic (Brocks et al., 2005; Scott et al., 2008; Lyons et al., 2009), despite the apparent persistence of iron-rich (ferruginous) conditions at depth (Planavsky et al., 2011; Poulton and Canfield, 2011). In addition to supporting greater anoxygenic production, euxinia may also increase the likelihood of nitrogenase metal cofactor limitation due to the burial of Mo in euxinic sediments (e.g., Anbar and Knoll, 2002)—even if euxinia was limited to only ~1–10% of the seafloor (Reinhard et al., 2013b). Regardless, because anaerobic respiration using SO42- as an electron acceptor (sulfate reduction) yields less energy than denitrification, evidence for euxinia is synonymous with evidence for NO3- depletion (Boyle et al., 2013). The observation that euxinia was relatively common in both space and time during the Proterozoic is therefore fully consistent with the conclusion that N stress may have been problematic for the Proterozoic biosphere, as well as the assertion that the Proterozoic biosphere may have been less oxygenic.

Oxygen-Nutrient Feedbacks

Whereas diazotrophy provides a pathway to alleviate N limitation, P is exclusively sourced to the ocean through crustal weathering. This inability of the marine biosphere to replenish P gives rise to the paradigm of a P-limited biosphere throughout Earth history (Redfield, 1958; Tyrrell, 1999). However, the assumption that cyanobacterial N2 fixation could keep pace with P supply during the Precambrian requires revisiting for reasons discussed above. In contrast with the prevailing view that P has limited marine productivity throughout Earth history, we hypothesize that prolonged N deficiency may have been a primary control on biospheric O2 fluxes and the tempo of Earth’s oxygenation.

As long as the ocean remained stratified with respect to O2, subsurface anoxia would have allowed a positive coupling between surface oxygenation and denitrification: nitrification is enhanced as surface oxygen oases expand, allowing greater loss of fixed N through subsequent subsurface denitrification—promoting N scarcity and providing a stabilizing negative feedback on biogenic O2 accumulation (Figure 1; also see Fennel et al., 2005). Complete alleviation of N scarcity via N2 fixation would be challenging for the reasons outlined above, which may result in reduced export production and organic C burial in response to oxygenation. Meanwhile, we argue that enhanced biospheric dependence on diazotrophy may also result in a reduction of net O2 production per mol organic C burial. Thus, minor oxygenation of a redox-stratified biosphere may strongly disfavor further oxygenation through the reduction of net biological O2 production.

The critical coupling between oxygenation and denitrification is reversed under O2-replete conditions. Following the oxygenation of the deep ocean, increases in O2 would tend to limit the areal extent of denitrifying conditions in the ocean, minimizing the likelihood of N stress and favoring P limitation (Fennel et al., 2005). Meanwhile, net O2 production would be expected to increase as the biospheric dependence on N2 fixation and the contribution of anoxygenic phototrophy to export production decreased. Thus, there may be two stable oxygen-nutrient states for the Earth system following the origin of oxygenic photosynthesis: an O2-stratified, N-limited biosphere and an O2-replete, P limited biosphere (Anbar and Knoll, 2002; Fennel et al., 2005). Indeed, updated C isotope models suggest a permanent increase in organic burial at the end of the Proterozoic (Krissansen-Totton et al., 2015), possibly consistent with the alleviation of N stress and the transition to a P-limited biosphere as the consequence of oxygenation in the late Neoproterozoic.

FIGURE 1
www.frontiersin.org

FIGURE 1. Nutrient-O2 feedbacks. Positive couplings are represented by solid blue arrows; negative couplings are represented by dashed red arrows. In the scenario shown here, minor surface oxygenation encourages nitrification, which allows greater subsurface denitrification (positive couplings). Loss of bioavailable N through denitrification in turn prompts greater N2 fixation—and we suggest that enhanced biospheric dependence on cyanobacterial diazotrophy results in reduced net O2 production (a negative coupling). The consequence is a stabilizing negative feedback: minor oxygenation results in reduced O2 production, strongly disfavoring O2 accumulation.

Concluding Remarks

Aerobic diazotrophy was a critical cyanobacterial innovation on par with oxygenic photosynthesis in terms of ecological impact, but it is likely that its environmental context and its contribution to global N cycling has varied dramatically through time. An important implication is that net biogenic O2 fluxes may have also varied through time. We hypothesize that low O2 availability may perpetuate low O2 conditions in the surface ocean, and anoxia at depth, by promoting extensive denitrification and the maintenance of conditions that are unfavorable for diazotrophy, both aerobic and anaerobic. Based on the success of anoxygenic diazotrophic cyanobacteria in the modern ocean, we further suggest that the most favorable cyanobacterial strategies for coping with N deficiency may have resulted in markedly reduced O2 production relative to today.

Enhanced biospheric dependence on cyanobacterial diazotrophy during the mid-Proterozoic, therefore, may explain the ~2.5 billion years delay between the Archean origin of oxygenic photosynthesis and the pervasive oxygenation of the ocean-atmosphere system in the Phanerozoic. Considering the likelihood of negative feedbacks that would tend to inhibit mitigation of global N-limitation, the relatively recent transition away from a world in which O2 accumulation was spatially limited to oases within the surface ocean toward a more pervasively oxygenated ocean may not be readily understood as the inevitable consequence of continued photosynthetic O2 production in surface environments. Instead, the transition from an O2-stratified, N-deficient marine biosphere to the broadly P-limited and well-oxygenated biosphere may require a yet unknown external perturbation to Earth’s O2 cycle. Although uncertainties remain, it is clear that cyanobacteria have played a highly dynamic role in the protracted oxygenation of the Earth system throughout the Precambrian—extending well beyond their invention of oxygenic photosynthesis roughly three or more billion years ago.

Author Contributions

SO wrote the paper with insight from all authors.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This material is based upon work supported by the National Aeronautics and Space Administration through the NASA Astrobiology Institute under Cooperative Agreement No. NNA15BB03A issued through the Science Mission Directorate. The authors also acknowledge support from the National Science Foundation Earth-Life Transitions program (TL and CR).

References

Anbar, A. D., Duan, Y., Lyons, T. W., Arnold, G. L., Kendall, B., Creaser, R. A., et al. (2007). A whiff of oxygen before the great oxidation event? Science 317, 1903–1906. doi: 10.1126/science.1140325

CrossRef Full Text | Google Scholar

Anbar, A. D., and Knoll, A. H. (2002). Proterozoic ocean chemistry and evolution: a bioinorganic bridge? Science 297, 1137–1142. doi: 10.1126/science.1069651

CrossRef Full Text | Google Scholar

Bekker, A., and Holland, H. D. (2012). Oxygen overshoot and recovery during the early Paleoproterozoic. Earth Planet. Sci. Lett. 317, 295–304. doi: 10.1016/j.epsl.2011.12.012

CrossRef Full Text | Google Scholar

Bergman, B., Gallon, J. R., Rai, A. N., and Stal, L. J. (1997). N2 fixation by non-heterocystous cyanobacteria. FEMS Microbiol. Rev. 19, 139–185. doi: 10.1016/S0168-6445(96)00028-9

CrossRef Full Text | Google Scholar

Bergman, B., Sandh, G., Lin, S., Larsson, J., and Carpenter, E. J. (2013). Trichodesmium—a widespread marine cyanobacteria with unusual nitrogen fixation properties. FEMS Microbiol. Rev. 37, 286–302. doi: 10.1111/j.1574-6976.2012.00352.x

CrossRef Full Text | Google Scholar

Berman-Frank, I., Lundgren, P., Chen, Y. B., Kupper, H., Kolber, Z., Bergman, B., et al. (2001). Segregation of nitrogen fixation and oxygenic photosynthesis in the marine cyanobacterium Trichodesmium. Science 294, 1534–1537. doi: 10.1126/science.1064082

CrossRef Full Text | Google Scholar

Berman-Frank, I., Lundgren, P., and Falkowski, P. (2003). Nitrogen fixation and photosynthetic oxygen evolution in cyanobacteria. Res. Microbiol. 154, 157–164. doi: 10.1016/S0923-2508(03)00029-9

CrossRef Full Text | Google Scholar

Bothe, H., Tripp, H. J., and Zehr, J. P. (2010). Unicellular cyanobacteria with a new mode of life: the lack of photosynthetic oxygen evolution allows nitrogen fixation to proceed. Arch. Microbiol. 192, 783–790. doi: 10.1007/s00203-010-0621-5

CrossRef Full Text | Google Scholar

Boyle, R. A., Clark, J. R., Poulton, S. W., Shields-Zhou, G., Canfield, D. E., and Lenton, T. M. (2013). Nitrogen cycle feedbacks as a control on euxinia in the mid-Proterozoic ocean. Nat. Commun. 4:1533. doi: 10.1038/ncomms2511

CrossRef Full Text | Google Scholar

Brocks, J. J., Love, G. D., Summons, R. E., Knoll, A. H., Logan, G. A., and Bowden, S. A. (2005). Biomarker evidence for green and purple sulphur bacteria in a stratified Palaeoproterozoic sea. Nature 437, 866–870. doi: 10.1038/nature04068

CrossRef Full Text | Google Scholar

Capone, D. G., Zehr, J. P., Paerl, H. W., Bergman, B., and Carpenter, E. J. (1997). Trichodesmium, a globally significant marine cyanobacterium. Science 276, 1221–1229. doi: 10.1126/science.276.5316.1221

CrossRef Full Text | Google Scholar

Deutsch, C., Sarmiento, J. L., Sigman, D. M., Gruber, N., and Dunne, J. P. (2007). Spatial coupling of nitrogen inputs and losses in the ocean. Nature 445, 163–167. doi: 10.1038/nature05392

CrossRef Full Text | Google Scholar

Fay, P. (1992). Oxygen relations of nitrogen fixation in cyanobacteria. Microbiol. Rev. 56, 340–373.

Google Scholar

Fennel, K., Follows, M., and Falkowski, P. G. (2005). The co-evolution of the nitrogen, carbon and oxygen cycles in the Proterozoic ocean. Am. J. Sci. 305, 526–545. doi: 10.2475/ajs.305.6-8.526

CrossRef Full Text | Google Scholar

Gallon, J. R. (1992). Reconciling the incompatible: N2 fixation and O2. New Phytol. 122, 571–609. doi: 10.1111/j.1469-8137.1992.tb00087.x

CrossRef Full Text | Google Scholar

Garvin, G., Buick, R., Anbar, A. D., Arnold, G. L., and Kaufman, A. J. (2009). Isotopic evidence for an aerobic nitrogen cycle in the latest Archean. Science 323, 1045–1048. doi: 10.1126/science.1165675

CrossRef Full Text | Google Scholar

Godfrey, L. V., and Falkowski, P. G. (2009). The cycling and redox state of nitrogen in the Archaean ocean. Nat. Geosci. 2, 725–729. doi: 10.1038/ngeo633

CrossRef Full Text | Google Scholar

Golubic, S., Sergeev, V. N., and Knoll, A. H. (1995). Mesoproterozoic archaeoellipsoides: akinetes of heterocystous cyanobacteria. Lethaia 28, 285–298. doi: 10.1111/j.1502-3931.1995.tb01817.x

CrossRef Full Text | Google Scholar

Gruber, N., and Sarmiento, J. L. (1997). Global patterns of marine nitrogen fixation and denitrification. Global Biogeochem. Cycles 11, 235–266. doi: 10.1073/pnas.1322132111

CrossRef Full Text | Google Scholar

Hamilton, T. L., Bryant, D. A., and Macalady, J. L. (2016). The role of biology in planetary evolution: cyanobacterial primary production in low-oxygen Proterozoic oceans. Environ. Microbiol. 18, 325–340. doi: 10.1111/1462-2920.13118

CrossRef Full Text | Google Scholar

Herman, E. K., and Kump, L. R. (2005). Biogeochemistry of microbial mats under Precambrian environmental conditions: a modeling study. Geobiology 3, 77–92. doi: 10.1111/j.1472-4669.2005.00048.x

CrossRef Full Text | Google Scholar

Holland, H. D. (2002). Volcanic gases, black smokers, and the great oxidation event. Geochim. Cosmochim. Acta 66, 3811–3862. doi: 10.1016/S0016-7037(02)00950-X

CrossRef Full Text | Google Scholar

Johnston, D. T., Wolfe-Simon, F., Pearson, A., and Knoll, A. H. (2009). Anoxygenic photosynthesis modulated Proterozoic oxygen and sustained Earth’s middle age. Proc. Natl Acad. Sci. U.S.A. 106, 16925–16929. doi: 10.1073/pnas.0909248106

CrossRef Full Text | Google Scholar

Kangatharalingam, N., Priscu, J. C., and Paerl, H. W. (1992). Heterocyst envelop thickness, heterocyst frequency, and nitrogenase activity in Anabaeana flos-aquae: influence of exogenous oxygen tension. J. Gen. Microbiol. 138, 2673–2678. doi: 10.1099/00221287-138-12-2673

CrossRef Full Text | Google Scholar

Kendall, B., Komiya, T., Lyons, T. W., Bates, S. M., Gordon, G. W., Romaniello, S. J., et al. (2015). Uranium and molybdenum isotope evidence for an episode of widespread ocean oxygenation during the late Ediacaran Period. Geochim. Cosmochim. Acta 156, 173–193. doi: 10.1016/j.gca.2015.02.025

CrossRef Full Text | Google Scholar

Kendall, B., Reinhard, C. T., Lyons, T. W., Kaufman, A. J., Poulton, S. W., and Anbar, A. D. (2010). Pervasive oxygenation along late Archaean ocean margins. Nat. Geosci. 3, 647–652. doi: 10.1038/ngeo942

CrossRef Full Text | Google Scholar

Krissansen-Totton, J., Buick, R., and Catling, D. C. (2015). A statistical analysis of the carbon isotope record from the Archean to Phanerozoic and implications for the rise of oxygen. Am. J. Sci. 315, 275–316. doi: 10.2475/04.2015.01

CrossRef Full Text | Google Scholar

Kuypers, M. M. M., Lavik, G., Woebken, D., Schmid, M., Fuchs, B. M., Amann, R., et al. (2005). Massive nitrogen loss from the Benguela upwelling system through anaerobic ammonium oxidation. Proc. Natl. Acad. Sci. U.S.A. 102, 6478–6483. doi: 10.1073/pnas.0502088102

CrossRef Full Text | Google Scholar

Lalonde, S. V., and Konhauser, K. O. (2015). Benthic perspective on Earth’s oldest evidence of oxygenic photosynthesis. Proc. Natl. Acad. Sci. U.S.A. 112, 995–1000. doi: 10.1073/pnas.1415718112

CrossRef Full Text | Google Scholar

Lyons, T. W., Anbar, A. D., Severmann, S., Scott, C., and Gill, B. C. (2009). Tracking euxinia in the ancient ocean: a multiproxy perspective and Proterozoic case study. Annu. Rev. Earth Planet. Sci. 37, 507–534. doi: 10.1146/annurev.earth.36.031207.124233

CrossRef Full Text | Google Scholar

Lyons, T. W., Reinhard, C. T., and Planavsky, N. J. (2014). The rise of oxygen in Earth’s early ocean and atmosphere. Nature 506, 307–315. doi: 10.1038/nature13068

CrossRef Full Text | Google Scholar

Murray, P. A., and Zinder, S. H. (1984). Nitrogen fixation by a methanogenic archaebacterium. Nature 312, 284–286. doi: 10.1038/312284a0

CrossRef Full Text | Google Scholar

Olson, S. L., Kump, L. R., and Kasting, J. F. (2013). Quantifying the areal extent and dissolved oxygen concentrations of Archean oxygen oases. Chem. Geol. 362, 35–43. doi: 10.1016/j.chemgeo.2013.08.012

CrossRef Full Text | Google Scholar

Partin, C. A., Bekker, A., Planavsky, N. J., Scott, C. T., Gill, B. C., Li, C., et al. (2013). Large-scale fluctuations in Precambrian atmospheric and oceanic oxygen levels from the record of U in shales. Earth Planet. Sci. Lett. 369, 284–293. doi: 10.1016/j.epsl.2013.03.031

CrossRef Full Text | Google Scholar

Planavsky, N. J., Asael, D., Hofmann, A., Reinhard, C. T., Lalonde, S. V., Knudsen, A., et al. (2014a). Evidence for oxygenic photosynthesis half a billion years before the great oxidation event. Nat. Geosci. 7, 283–286. doi: 10.1038/ngeo2122

CrossRef Full Text | Google Scholar

Planavsky, N. J., Bekker, A., Hofmann, A., Owens, J. D., and Lyons, T. W. (2012). Sulfur record of rising and falling marine oxygen and sulfate levels during the Lomagundi event. Proc. Natl. Acad. Sci. U.S.A. 109, 18300–18305. doi: 10.1073/pnas.1120387109

CrossRef Full Text | Google Scholar

Planavsky, N. J., McGoldrick, P., Scott, C. T., Reinhard, C. T., Kelly, A. E., Chu, X., et al. (2011). Widespread iron-rich conditions in the mid-Proterozoic ocean. Nature 477, 448–452. doi: 10.1038/nature10327

CrossRef Full Text | Google Scholar

Planavsky, N. J., Reinhard, C. T., Wang, X., Thomson, D., McGoldrick, P., Rainbird, R. H., et al. (2014b). Low mid-proterozoic atmospheric oxygen levels and the delayed rise of animals. Science 346, 635–638. doi: 10.1126/science.1258410

CrossRef Full Text | Google Scholar

Poulton, S. W., and Canfield, D. E. (2011). Ferruginous conditions: a dominant feature of the ocean through Earth’s history. Elements 7, 107–112. doi: 10.2113/gselements.7.2.107

CrossRef Full Text | Google Scholar

Redfield, A. C. (1958). The biological control of chemical factors in the environment. Am. Sci. 46, 205–221.

Google Scholar

Reinhard, C. T., Lalonde, S. V., and Lyons, T. W. (2013a). Oxidative sulfide dissolution on the early Earth. Chem. Geol. 362, 44–55. doi: 10.1016/j.chemgeo.2013.10.006

CrossRef Full Text | Google Scholar

Reinhard, C. T., Planavsky, N. J., Olson, S. L., Lyons, T. W., and Erwin, D. H. (2016). Earth’s oxygen cycle and the evolution of metazoan life. Proc. Natl. Acad. Sci. U.S.A. 113, 8933–8938. doi: 10.1073/pnas.1521544113

CrossRef Full Text | Google Scholar

Reinhard, C. T., Planavsky, N. J., Robbins, L. J., Partin, C. A., Gill, B. C., Lalonde, S. V., et al. (2013b). Proterozoic ocean redox and biogeochemical stasis. Proc. Natl. Acad. Sci. U.S.A. 110, 5357–5362. doi: 10.1073/pnas.1208622110

CrossRef Full Text | Google Scholar

Rippka, R., and Stanier, R. Y. (1978). The effects of anaerobiosis on nitrogenase synthesis and heterocyst development by nosocacean cyanobacteria. J. Gen. Microbiol. 105, 83–94. doi: 10.1099/00221287-105-1-83

CrossRef Full Text | Google Scholar

Rybacki, K. S., Kump, L. R., Hanski, E. J., and Melezhik, V. A. (2016). Weathering during the great oxidation event: fennoscandia arctic Russia 2.06 Ga ago. Precambrian Res. 275, 513–525. doi: 10.1016/j.precamres.2016.01.010

CrossRef Full Text | Google Scholar

Sahoo, S. K., Planavsky, N. J., Kendall, B., Wang, W., Shi, X., Scott, C., et al. (2012). Ocean oxygenation in the wake of the Marinoan glaciation. Nature 489, 546–549. doi: 10.1038/nature11445

CrossRef Full Text | Google Scholar

Scott, C. T., Bekker, A., Reinhard, C. T., Schnetger, B., Krapez, B., Rumble, D. I. I. I., et al. (2011). Late Archean euxinic conditions before the rise of atmospheric oxygen. Geology 39, 119–122. doi: 10.1126/science.1176711

CrossRef Full Text | Google Scholar

Scott, C. T., Lyons, T. W., Bekker, A., Shen, Y., Poulton, S. W., Chu, X., et al. (2008). Tracing the stepwise oxygenation of the Proterozoic ocean. Nature 452, 456–460. doi: 10.1038/nature06811

CrossRef Full Text | Google Scholar

Sperling, E. A., Wolock, C. J., Morgan, A. S., Gill, B. C., Kunzmann, M., Halverson, G. P., et al. (2015). Statistical analysis of iron geochemical data suggests limited late Proterozoic oxygenation. Nature 523, 451–454. doi: 10.1038/nature14589

CrossRef Full Text | Google Scholar

Stal, L. J. (2009). Is the distribution of nitrogen-fixing cyanobacteria in the oceans related to temperature? Environ. Microbiol. 11, 1632–1645. doi: 10.1111/j.1758-2229.2009.00016.x

CrossRef Full Text | Google Scholar

Sumner, D. Y., Hawes, I., Mackey, T. J., Jungblut, A. D., and Doran, P. T. (2015). Antarctic microbial mats: a modern analog for Archean lacustrine oxygen oases. Geology 43, 887–890. doi: 10.1130/G36966.1

CrossRef Full Text | Google Scholar

Tomitani, A., Knoll, A. H., Cavanaugh, C. M., and Ohno, T. (2006). The evolutionary diversification of cyanobacteria: molecular-phylogenetic and paleontological perspectives. Proc. Natl. Acad. Sci. U.S.A. 103, 5442–5447. doi: 10.1073/pnas.0600999103

CrossRef Full Text | Google Scholar

Tripp, H. J., Bench, S. R., Turk, K. A., Foster, R. A., Desany, B. A., Niazi, F., et al. (2010). Metabolic streamlining in an open-ocean nitrogen-fixing cyanobacterium. Nature 464, 90–94. doi: 10.1038/nature08786

CrossRef Full Text | Google Scholar

Tyrrell, T. (1999). The relative influences of nitrogen and phosphorus on oceanic primary production. Nature 400, 525–531. doi: 10.1038/22941

CrossRef Full Text | Google Scholar

Zehr, J. P. (2011). Nitrogen fixation by marine cyanobacteria. Trends Microbiol. 19, 162–173. doi: 10.1016/j.tim.2010.12.004

CrossRef Full Text | Google Scholar

Zehr, J. P., Bench, S. R., Carter, B. J., Hewson, I., Niazi, F., Shi, T., et al. (2008). Globally distributed oceanic N2-fixing cyanobacteria lack oxygenic photosystem II. Science 322, 1110–1112. doi: 10.1126/science.1165340

CrossRef Full Text | Google Scholar

Keywords: cyanobacteria, oxygen, denitrification, diazotrophy, boring billion

Citation: Olson SL, Reinhard CT and Lyons TW (2016) Cyanobacterial Diazotrophy and Earth’s Delayed Oxygenation. Front. Microbiol. 7:1526. doi: 10.3389/fmicb.2016.01526

Received: 30 June 2016; Accepted: 12 September 2016;
Published: 23 September 2016.

Edited by:

Robert Eugene Blankenship, Washington University in St. Louis, USA

Reviewed by:

John Stolz, Duquesne University, USA
Katja Meyer, Willamette University, USA

Copyright © 2016 Olson, Reinhard and Lyons. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Stephanie L. Olson, solso002@ucr.edu

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.