%A Kalantari,Aida %A Chen,Tao %A Ji,Boyang %A Stancik,Ivan A. %A Ravikumar,Vaishnavi %A Franjevic,Damjan %A Saulou-BĂ©rion,Claire %A Goelzer,Anne %A Mijakovic,Ivan %D 2017 %J Frontiers in Microbiology %C %F %G English %K 3-hydroxypropanoic acid,Glycerol,Bacillus subtilis,Metabolic Engineering,glycerol kinase knock-out %Q %R 10.3389/fmicb.2017.00638 %W %L %M %P %7 %8 2017-April-18 %9 Original Research %+ Ivan Mijakovic,Systems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology,Gothenburg, Sweden,ivan.mijakovic@chalmers.se %+ Ivan Mijakovic,Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark,Lyngby, Denmark,ivan.mijakovic@chalmers.se %# %! 3HP production in B. subtilis %* %< %T Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis %U https://www.frontiersin.org/articles/10.3389/fmicb.2017.00638 %V 8 %0 JOURNAL ARTICLE %@ 1664-302X %X 3-Hydroxypropanoic acid (3-HP) is an important biomass-derivable platform chemical that can be converted into a number of industrially relevant compounds. There have been several attempts to produce 3-HP from renewable sources in cell factories, focusing mainly on Escherichia coli, Klebsiella pneumoniae, and Saccharomyces cerevisiae. Despite the significant progress made in this field, commercially exploitable large-scale production of 3-HP in microbial strains has still not been achieved. In this study, we investigated the potential of Bacillus subtilis as a microbial platform for bioconversion of glycerol into 3-HP. Our recombinant B. subtilis strains overexpress the two-step heterologous pathway containing glycerol dehydratase and aldehyde dehydrogenase from K. pneumoniae. Genetic engineering, driven by in silico optimization, and optimization of cultivation conditions resulted in a 3-HP titer of 10 g/L, in a standard batch cultivation. Our findings provide the first report of successful introduction of the biosynthetic pathway for conversion of glycerol into 3-HP in B. subtilis. With this relatively high titer in batch, and the robustness of B. subtilis in high density fermentation conditions, we expect that our production strains may constitute a solid basis for commercial production of 3-HP.