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Original Research ARTICLE

Front. Neuroanat., 07 August 2012 | http://dx.doi.org/10.3389/fnana.2012.00030

Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas

Houri Hintiryan, Lin Gou, Brian Zingg, Seita Yamashita, Hannah M. Lyden, Monica Y. Song, Arleen K. Grewal, Xinhai Zhang, Arthur W. Toga and Hong-Wei Dong*
  • Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA

We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

Keywords: main olfactory bulb, piriform cortical area, lateral olfactory tract, online digital atlas, connectome, neural tract tracing

Citation: Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW and Dong H-W (2012) Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas. Front. Neuroanat. 6:30. doi: 10.3389/fnana.2012.00030

Received: 09 May 2012; Paper pending published: 26 May 2012;
Accepted: 19 July 2012; Published online: 07 August 2012.

Edited by:

Jorge A. Larriva-Sahd, Universidad Nacional Autónoma de México, Mexico

Reviewed by:

Marco Aurelio M. Freire, Edmond and Lily Safra International Institute for Neurosciences of Natal, Brazil
Juan Andrés De Carlos, Instituto Cajal (Consejo Superior de Investigaciones Científicas), Spain

Copyright © 2012 Hintiryan, Gou, Zingg, Yamashita, Lyden, Song, Grewal, Zhang, Toga and Dong. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

*Correspondence: Hong-Wei Dong, Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 635 Charles E. Young Drive South, Suite 225, Los Angeles, CA 90095-7334, USA. e-mail: hongwei.dong@loni.ucla.edu