Impact Factor

Original Research ARTICLE

Front. Behav. Neurosci., 26 November 2013 | http://dx.doi.org/10.3389/fnbeh.2013.00173

Optogenetic stimulation of VTA dopamine neurons reveals that tonic but not phasic patterns of dopamine transmission reduce ethanol self-administration

  • 1Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA
  • 2Department of Neurobiology and Anatomy, Wake Forest School of Medicine, Winston-Salem, NC, USA
  • 3Department of Physiology and Pharmacology, Wake Forest School of Medicine, Winston-Salem, NC, USA
  • 4Department of Physics, Wake Forest University, Winston-Salem, NC, USA
  • 5Departments of Psychiatry and Cell Biology and Physiology, Neuroscience Center and Bowles Center for Alcohol Studies, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

There is compelling evidence that acute ethanol exposure stimulates ventral tegmental area (VTA) dopamine cell activity and that VTA-dependent dopamine release in terminal fields within the nucleus accumbens plays an integral role in the regulation of ethanol drinking behaviors. Unfortunately, due to technical limitations, the specific temporal dynamics linking VTA dopamine cell activation and ethanol self-administration are not known. In fact, establishing a causal link between specific patterns of dopamine transmission and ethanol drinking behaviors has proven elusive. Here, we sought to address these gaps in our knowledge using a newly developed viral-mediated gene delivery strategy to selectively express Channelrhodopsin-2 (ChR2) on dopamine cells in the VTA of wild-type rats. We then used this approach to precisely control VTA dopamine transmission during voluntary ethanol drinking sessions. The results confirmed that ChR2 was selectively expressed on VTA dopamine cells and delivery of blue light pulses to the VTA induced dopamine release in accumbal terminal fields with very high temporal and spatial precision. Brief high frequency VTA stimulation induced phasic patterns of dopamine release in the nucleus accumbens. Lower frequency stimulation, applied for longer periods mimicked tonic increases in accumbal dopamine. Notably, using this optogenetic approach in rats engaged in an intermittent ethanol drinking procedure, we found that tonic, but not phasic, stimulation of VTA dopamine cells selectively attenuated ethanol drinking behaviors. Collectively, these data demonstrate the effectiveness of a novel viral targeting strategy that can be used to restrict opsin expression to dopamine cells in standard outbred animals and provide the first causal evidence demonstrating that tonic activation of VTA dopamine neurons selectively decreases ethanol self-administration behaviors.

Keywords: dopamine, VTA, nucleus accumbens, optogenetics, ethanol self-administration

Citation: Bass CE, Grinevich VP, Gioia D, Day-Brown JD, Bonin KD, Stuber GD, Weiner JL and Budygin EA (2013) Optogenetic stimulation of VTA dopamine neurons reveals that tonic but not phasic patterns of dopamine transmission reduce ethanol self-administration. Front. Behav. Neurosci. 7:173. doi: 10.3389/fnbeh.2013.00173

Received: 08 October 2013; Accepted: 05 November 2013;
Published online: 26 November 2013.

Edited by:

Anton Ilango, National Institutes of Health, USA

Reviewed by:

Alicia Izquierdo, University of California, Los Angeles, USA
Raul Gainetdinov, Italian Institute of Technology, Italy

Copyright © 2013 Bass, Grinevich, Gioia, Day-Brown, Bonin, Stuber, Weiner and Budygin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Evgeny A. Budygin, Department of Neurobiology and Anatomy, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157, USA e-mail: ebudygin@wakehealth.edu