Multiple birthdating analyses in adult neurogenesis: a line-up of the usual suspects
- Department of Molecular, Cellular and Developmental Neurobiology, Cajal Institute, Madrid, Spain
Analyzing the variation in different subpopulations of newborn neurons is central to the study of adult hippocampal neurogenesis. The acclaimed working hypothesis that different subpopulations of newborn, differentiating neurons could be playing different roles arouses great interest. Therefore, the physiological and quantitative analysis of neuronal subpopulations at different ages is critical to studies of neurogenesis. Such approaches allow cells of different ages to be identified by labeling them according to their probable date of birth. Until very recently, only neurons born at one specific time point could be identified in each experimental animal. However the introduction of different immunohistochemically compatible markers now enables multiple subpopulations of newborn neurons to be analyzed in the same animal as in a line-up, revealing the relationships between these subpopulations in response to specific influences or conditions. This review summarizes the current research carried out using these techniques and outlines some of the key applications.
Keywords: neurogenesis, dentate gyrus, dual birthdating, CldU, IdU, BrdU
Citation: Llorens-Martín M and Trejo JL (2011) Multiple birthdating analyses in adult neurogenesis: a line-up of the usual suspects. Front. Neurosci. 5:76. doi: 10.3389/fnins.2011.00076
Received: 20 January 2011;
Accepted: 17 May 2011;
Published online: 27 May 2011.
Edited by:Silvia De marchis, University of Turin, Italy
Reviewed by:Muriel Koehl, Institut national de la santé et de la recherche médicale; (Inserm), France
Helena Mira, Instituto de Salud Carlos III, Spain
Copyright: © 2011 Llorens-Martín and Trejo. This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.
*Correspondence: José L. Trejo, Department of Molecular, Cellular and Developmental Neurobiology, Cajal Institute, Av. Doctor Arce, 37, 28002 Madrid, Spain. e-mail: email@example.com