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This article is part of the Research Topic Targeting PI3K/mTOR signaling in cancer

Review ARTICLE

Front. Oncol., 16 October 2012 | http://dx.doi.org/10.3389/fonc.2012.00145

Abrogating endocrine resistance by targeting ERα and PI3K in breast cancer

Emily M. Fox1, Carlos L. Arteaga1,2,3 and Todd W. Miller4*
  • 1Department of Medicine, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, TN, USA
  • 2Department of Cancer Biology, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, TN, USA
  • 3Breast Cancer Research Program, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, TN, USA
  • 4Department of Pharmacology and Toxicology, Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA

Antiestrogen therapies targeting estrogen receptor α (ER) signaling are a mainstay for patients with ER+ breast cancer. While many cancers exhibit resistance to antiestrogen therapies, a large body of clinical and experimental evidence indicates that hyperactivation of the phosphatidylinositol 3-kinase (PI3K) pathway promotes antiestrogen resistance. In addition, continued ligand-independent ER signaling in the setting of estrogen deprivation may contribute to resistance to endocrine therapy. PI3K activates several proteins which promote cell cycle progression and survival. In ER+ breast cancer cells, PI3K promotes ligand-dependent and -independent ER transcriptional activity. Models of antiestrogen-resistant breast cancer often remain sensitive to estrogen stimulation and PI3K inhibition, suggesting that clinical trials with combinations of drugs targeting both the PI3K and ER pathways are warranted. Herein, we review recent findings on the roles of PI3K and ER in antiestrogen resistance, and clinical trials testing drug combinations which target both pathways. We also discuss the need for clinical investigation of ER downregulators in combination with PI3K inhibitors.

Introduction

At least 75% of breast cancers express estrogen receptor α (ER) and/or progesterone receptor (PR), which are tumor biomarkers of estrogen dependence. Antiestrogen treatments for patients with ER+ or PR+ breast cancer inhibit ER by antagonizing estrogen ligand binding to ER (tamoxifen and other selective estrogen receptor modulators, SERMs), inhibiting dimerization and downregulating ER (fulvestrant and other selective estrogen receptor downregulators, SERDs), or blocking estrogen production (aromatase inhibitors, AIs; letrozole, anastrozole, exemestane). While such endocrine therapies have changed the natural history of ER+ breast cancer, many tumors exhibit de novo or acquired drug resistance (Table 1). The only clinically validated mechanism of resistance to endocrine therapy is overexpression or amplification of the ERBB2 (HER2) protooncogene (Arpino et al., 2004; De Laurentiis et al., 2005; Ellis et al., 2006). However, only 10% of ER+ breast cancers exhibit HER2 overexpression, prompting the need for discovery of other mechanisms of antiestrogen resistance.

TABLE 1
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TABLE 1. Frequencies of breast cancer recurrence and resistance to anti-estrogen therapies in patients with ER+ breast cancer.

A large body of experimental and clinical evidence suggests that hyperactivation of the phosphatidylinositol 3-kinase (PI3K) pathway, the most frequently mutated pathway in breast cancer, promotes antiestrogen resistance. PI3K is commonly activated by growth factor receptor tyrosine kinases and G-protein-coupled receptors in breast cancer cells. The signaling cascades triggered by PI3K, including PDK1, AKT, and SGK among others, promote cell growth and survival. For detailed information, we refer the reader to a recently published, comprehensive review of this material (Miller et al., 2011a). Herein, we focus on updated findings, clinical testing of drug combinations targeting the ER and PI3K pathways, and the need to clinically address the potential for continued ER signaling in patients treated with endocrine therapies.

Rationale for Combined Targeting of the ER and PI3K Pathways

We and others identified a requirement for PI3K in the estrogen-independent growth of long-term estrogen-deprived (LTED) ER+ breast cancer cells, which mirror clinical resistance to AIs (Sabnis et al., 2007; Crowder et al., 2009; Miller et al., 2010). Proteomic profiling revealed amplification of PI3K signaling via the mTOR substrates p70S6 kinase and p85S6 kinase, and the PI3K effector AKT in ER+ human breast cancer cells adapted to hormone deprivation. Treatment with the ATP-competitive PI3K/mTOR dual inhibitor BEZ235 (Maira et al., 2008) completely suppressed the emergence of hormone-independent ER+ cells and induced apoptosis in cell lines harboring activating mutations in PIK3CA (gene that encodes the p110α subunit of PI3K) or PTEN loss (PTEN antagonizes PI3K signaling). In contrast, the TORC1 inhibitor everolimus (Schuler et al., 1997) had only a partial effect (Miller et al., 2010; Sanchez et al., 2011). This partial effect may be attributable to feedback activation of PI3K/AKT upon inhibition of TORC1 (O’Reilly et al., 2006; Carracedo et al., 2008; Miller et al., 2009), suggesting that direct inhibitors of PI3K may be more effective than rapalogs in this setting.

In a siRNA screen against 779 kinases, we implicated insulin receptor (InsR) in the hormone-independent growth of MCF-7/LTED cells. InsR and its homolog IGF-1R dimerize and, upon ligand binding, potently activate PI3K. IGF-1R has also been shown to confer antiestrogen resistance in MCF-7 cells (Zhang et al., 2011). Treatment with the ATP-competitive IGF-1R/InsR inhibitor OSI-906 suppressed PI3K activation and hormone-independent ER+ cell growth (Fox et al., 2011). Network mapping of the 42 kinases individually implicated in MCF-7/LTED cell growth in this screen revealed that PI3K is a central hub in these signaling pathways (Figure 1). Interestingly, a recent study showed that in ER+ breast cancer cells treated with BEZ235 or with PI3K siRNA, exogenous 17β-estradiol rescued the cells from drug- and siRNA-induced apoptosis (Crowder et al., 2009; Sanchez et al., 2011). This suggests that in ER+ cancers treated with PI3K inhibitors, estrogen suppression should be maintained and, therefore, combined inhibition of both PI3K and ER may be more effective than single-agent therapies.

FIGURE 1
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FIGURE 1. Phosphatidylinositol 3-kinase is a central hub in signaling pathways required for estrogen-independent ER+ breast cancer cell growth. MCF-7/LTED cells transiently transfected with a siRNA library targeting 779 kinases were reseeded in hormone-depleted medium. Cell viability was measured 4–5 days later by Alamar blue assay. Median cell growth in four independent experiments was calculated for each kinase siRNA relative to non-silencing controls. Individual knockdown of 42 kinases inhibited MCF-7/LTED cell growth ≥33% (p ≤ 0.05) in at least three of four experiments (detailed in Fox et al., 2011). Ingenuity Pathways Analysis revealed that these 42 kinases map to several protein networks that overlap with PI3K signaling (red box, enlarged in bottom panel). Proteins involved in these networks are displayed as nodes. Solid lines indicate direct relationships between proteins, and dotted lines indicate indirect interactions. Green nodes represent the kinases identified in the screen, as well as others whose knockdown was predicted by the Ingenuity software to negatively affect cell growth. The various nodal shapes represent the functional class of the gene product.

Clinical evidence further indicates that PI3K pathway activation is associated with antiestrogen resistance. Patients bearing primary ER+ breast tumors which exhibit a protein expression/phosphorylation signature of PI3K activation, as determined using reverse-phase protein arrays (RPPA), have a shorter recurrence-free survival (Miller et al., 2010). RPPA analysis of ER+ primary breast tumors obtained from patients following 2–3 weeks of treatment with the AI letrozole showed that a protein signature of insulin signaling was associated with high post-AI tumor cell proliferation (Fox et al., 2011). Overexpression of HER2 or FGFR1, or loss of INPP4B, molecular lesions which activate the PI3K pathway, also confer antiestrogen resistance in patients with ER+ breast cancer (Arpino et al., 2004; De Laurentiis et al., 2005; Ellis et al., 2006; Gewinner et al., 2009; Turner et al., 2010). Also noteworthy is the inverse correlation between levels of PI3K activation and ER protein in human tumors. This ER/PI3K balance can be shifted using PI3K and ER inhibitors in preclinical models (Figure 2; Creighton et al., 2010; Miller et al., 2010), suggesting that cells may defer to the other pathway when one is inhibited.

FIGURE 2
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FIGURE 2. Estrogen receptor inhibition with fulvestrant induces upregulation of PI3K signaling. Ovariectomized athymic mice were s.c. implanted with MCF-7 cells and a 10-day-release E2 pellet (0.12 mg). Twelve days later, mice were randomized to treatment with vehicle or fulvestrant (5 mg/week, s.c., clinical formulation). Tumors were harvested after 3–4 weeks of treatment. Tumor lysates were analyzed by immunoblotting using the indicated antibodies; each lane contains equal amount of protein from two to three tumors. Fulvestrant treatment decreased the levels of ER and ER-regulated genes products (PR, IGF-1R), but increased levels of P-AKT-T308 and P-AKT-S473, suggesting increased activation of PI3K. All lanes were from the same membrane.

Crosstalk between the PI3K and ER pathways has also been suggested as a mechanism of endocrine resistance (Musgrove and Sutherland, 2009). PI3K activation was shown to induce ER phosphorylation at the putative AKT/p70S6K site Ser167 and estrogen-independent transcriptional activity (Campbell et al., 2001; Yamnik et al., 2009). However, treatment of such cells in hormone-depleted conditions with everolimus or the pan-PI3K inhibitor BKM120 (Maira et al., 2012) did not decrease ER phosphorylation at Ser167, ER-DNA binding, or ER transcriptional reporter activity (Miller et al., 2011b). These data collectively suggest that PI3K effectors do not modulate ER in the absence of estrogens. Analysis of the effects of BKM120 and fulvestrant on hormone-independent cell growth showed synergy in 6/8 ER+ lines. In mice bearing ER+ breast cancer xenografts, single-agent treatment with BKM120 or fulvestrant slowed tumor growth, while the combination induced tumor regression. Similarly, treatment with the ATP-competitive IGF-1R/InsR dual inhibitor OSI-906, which blocks downstream activation of PI3K in MCF-7 cells, slowed tumor growth and induced regression when combined with fulvestrant (Fox et al., 2011). These data further imply that combined targeting of the ER and PI3K pathways is more effective than single-agent therapies.

Clinical Trials Testing Drug Combinations Targeting the ER and PI3K Pathways

Herein, we will review three recent clinical studies that evaluated the benefit of adding the TORC1 inhibitor everolimus to endocrine therapy. (1) In the first study, post-menopausal women with early-stage ER+ breast cancer were randomized to neoadjuvant therapy with the AI letrozole ± everolimus for 4 months. The addition of everolimus increased clinical response and suppression of tumor cell proliferation at 2 weeks (measured by Ki67 IHC) compared to letrozole alone (Baselga et al., 2009). (2) In the TAMRAD study, post-menopausal patients with metastatic, ER+, AI-resistant breast cancer were randomized to treatment with tamoxifen ± everolimus. The addition of everolimus improved clinical benefit rate, time-to-progression, and disease-free survival compared to tamoxifen alone (Bachelot et al., 2010). (3) The phase III BOLERO-2 study included 724 post-menopausal women with metastatic, ER+, HER2-negative breast cancer. While 84% of patients exhibited sensitivity to prior endocrine therapy, all were resistant to non-steroidal AIs (letrozole, anastrozole) at the time of randomization to treatment with the steroidal AI exemestane ± everolimus. The addition of everolimus increased progression-free survival (PFS) from 4.1 months (exemestane alone) to 10.6 months (Baselga et al., 2012).

While the addition of a TORC1 inhibitor prevents disease progression in patients with antiestrogen-resistant breast cancer, inhibition of TORC1 relieves negative feedback on activators of PI3K (e.g., IGF-1R, IRS-1, HER3; O’Reilly et al., 2006; Carracedo et al., 2008; Miller et al., 2009). These data suggest that direct inhibitors of PI3K may be more effective. Early clinical testing of PI3K inhibitors in combination with antiestrogens suggests that this strategy is feasible. In a phase Ib trial, post-menopausal patients with advanced ER+ disease are being treated with letrozole plus the PI3K inhibitor BKM120. This drug combination is safe and exhibits promising anti-tumor activity (Mayer et al., 2012).

Rationale for an ER Downregulator in Combination with a PI3K Inhibitor in AI-Resistant Breast Cancer

A recent comparison of high-dose fulvestrant (an ER downregulator) to the AI anastrozole as first-line treatment for advanced breast cancer revealed that fulvestrant provided a longer time-to-progression (Robertson et al., 2009a). In other studies, ~35% of patients who progressed on an AI responded to second-line fulvestrant (Ingle et al., 2006; Perey et al., 2007). This suggests that in some clinical situations, downregulation of ER may be superior to estrogen deprivation (AI) therapy (Robertson et al., 2009a). We recently reported that ER retains transcriptional activity in estrogen-independent LTED cells and primary human breast tumors (i.e., following AI therapy), and drives the estrogen-independent growth of LTED cells (Miller et al., 2011b). These data suggest that estrogen (ligand)-independent ER activity may promote resistance to AI therapy. While their side effect profiles are generally similar, AI therapy increases the risk of bone fractures and joint disorders more so than fulvestrant (Howell et al., 2002, 2005; Osborne et al., 2002; Goss et al., 2003; Robertson et al., 2003; Howell and Sapunar, 2011). Fulvestrant, which is administered intramuscularly, is associated with injection site pain, and only induces partial ER downregulation in tumors (Robertson et al., 2009b). Hence, the development of a more potent, orally available ER downregulator/inhibitor may provide a convenient and effective treatment option for patients with ER+ breast cancer.

Cancer cells harboring activating mutations in PIK3CA exhibit increased sensitivity to PI3K inhibition (Miller et al., 2010; Sanchez et al., 2011; Maira et al., 2012), suggesting that this class of drugs may be most effective against tumors with mutations in the PI3K pathway. In mice bearing ER+, HER2-negative, PIK3CA-mutant MCF-7 breast cancer xenografts, treatment with the combination of fulvestrant and BKM120 induced tumor regression (Miller et al., 2011b). Using [18F]FDG-PET imaging as an early biomarker of metabolic inhibition, treatment with BKM120 but not fulvestrant decreased tumor FDG uptake. BKM120 increased tumor cell apoptosis, while fulvestrant decreased tumor cell proliferation. These findings may be validated clinically in a phase II clinical trial where post-menopausal patients with AI-resistant, ER+, HER2-negative, PIK3CA-mutant breast cancer are randomized to treatment with another AI plus a PI3K inhibitor vs. fulvestrant plus a PI3K inhibitor (Figure 3). The novel agent in such a trial would be the PI3K inhibitor, but the comparison would be an AI vs. fulvestrant. The primary endpoint would be PFS. Incorporation of non-invasive imaging with [18F]FDG-PET at baseline and after several weeks of treatment could identify metabolic changes indicative of a pharmacodynamic effect. This comparison would inform us whether (1) the addition of a PI3K inhibitor to an AI is beneficial, (2) downregulation of ER is superior to estrogen deprivation (AI) therapy in the context of PI3K inhibition, and (3) metabolic inhibition at an early time point as reflected by FDG-PET is predictive of PFS.

FIGURE 3
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FIGURE 3. Diagram of a clinical trial with a PI3K pathway inhibitor in AI-resistant breast cancer. Patients with breast cancer that progressed on AI therapy will be subjected to a biopsy to confirm ER+, HER2-negative, PIK3CA-mutant status. Eligible patients would then be randomized to another AI plus a PI3K inhibitor, or fulvestrant plus the PI3K inhibitor. FDG-PET scans would be performed before and after 4 weeks of therapy to identify early metabolic changes. Patients will be treated until progression.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This work was supported by the National Institutes of Health K99CA142899, R00CA142899 (Todd W. Miller), Breast Cancer Specialized Program of Research Excellence (SPORE) P50CA98131, Vanderbilt-Ingram Cancer Center Support Grant P30CA68485; a grant from the Breast Cancer Research Foundation (Carlos L. Arteaga); American Cancer Society Clinical Research Professorship Grant CRP-07-234 (Carlos L. Arteaga) and Postdoctoral Fellowship Grant PF-10-184-01-TBE (Emily M. Fox); the Lee Jeans Translational Breast Cancer Research Program (Carlos L. Arteaga); and Stand Up to Cancer/American Association for Cancer Research Dream Team Translational Cancer Research Grant SU2C-AACR-DT0209 (Carlos L. Arteaga).

References

Arpino, G., Green, S. J., Allred, D. C., Lew, D., Martino, S., Osborne, C. K., et al. (2004). HER-2 amplification, HER-1 expression, and tamoxifen response in estrogen receptor-positive metastatic breast cancer: a southwest oncology group study. Clin. Cancer Res. 10, 5670–5676.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Bachelot, T., Bourgier, C., Cropet, C., Guastalla, J.-P., Ferrero, J.-M., Leger-Falandry, C., et al. (2010). TAMRAD: a GINECO randomized phase II trial of everolimus in combination with tamoxifen versus tamoxifen alone in patients (pts) with hormone-receptor positive, HER2 negative metastatic breast cancer (MBC) with prior exposure to aromatase inhibitors (AI). Cancer Res. 70, abstract S1–S6.

CrossRef Full Text

Baselga, J., Campone, M., Piccart, M., Burris, H. A. III, Rugo, H. S., Sahmoud, T., et al. (2012). Everolimus in postmenopausal hormone-receptor-positive advanced breast cancer. N. Engl. J. Med. 366, 520–529.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Baselga, J., Semiglazov, V., Van Dam, P., Manikhas, A., Bellet, M., Mayordomo, J., et al. (2009). Phase II randomized study of neoadjuvant everolimus plus letrozole compared with placebo plus letrozole in patients with estrogen receptor-positive breast cancer. J. Clin. Oncol. 27, 2630–2637.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Campbell, R. A., Bhat-Nakshatri, P., Patel, N. M., Constantinidou, D., Ali, S., and Nakshatri, H. (2001). Phosphatidylinositol 3-kinase/AKT-mediated activation of estrogen receptor alpha: a new model for anti-estrogen resistance. J. Biol. Chem. 276, 9817–9824.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Carracedo, A., Ma, L., Teruya-Feldstein, J., Rojo, F., Salmena, L., Alimonti, A., et al. (2008). Inhibition of mTORC1 leads to MAPK pathway activation through a PI3K-dependent feedback loop in human cancer. J. Clin. Invest. 118, 3065–3074.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Chia, S., Gradishar, W., Mauriac, L., Bines, J., Amant, F., Federico, M., et al. (2008). Double-blind, randomized placebo controlled trial of fulvestrant compared with exemestane after prior nonsteroidal aromatase inhibitor therapy in postmenopausal women with hormone receptor-positive, advanced breast cancer: results from EFECT. J. Clin. Oncol. 26, 1664–1670.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Creighton, C. J., Fu, X., Hennessy, B. T., Casa, A. J., Zhang, Y., Gonzalez-Angulo, A. M., et al. (2010). Proteomic and transcriptomic profiling reveals a link between the PI3K pathway and lower estrogen receptor (ER) levels and activity in ER+ breast cancer. Breast Cancer Res. 12, R40.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Crowder, R. J., Phommaly, C., Tao, Y., Hoog, J., Luo, J., Perou, C. M., et al. (2009). PIK3CA and PIK3CB inhibition produce synthetic lethality when combined with estrogen deprivation in estrogen receptor-positive breast cancer. Cancer Res. 69, 3955–3962.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Cuzick, J., Sestak, I., Baum, M., Buzdar, A., Howell, A., Dowsett, M., et al. (2010). Effect of anastrozole and tamoxifen as adjuvant treatment for early-stage breast cancer: 10-year analysis of the ATAC trial. Lancet Oncol. 11, 1135–1141.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

De Laurentiis, M., Arpino, G., Massarelli, E., Ruggiero, A., Carlomagno, C., Ciardiello, F., et al. (2005). A meta-analysis on the interaction between HER-2 expression and response to endocrine treatment in advanced breast cancer. Clin. Cancer Res. 11, 4741–4748.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ellis, M. J., Tao, Y., Young, O., White, S., Proia, A. D., Murray, J., et al. (2006). Estrogen-independent proliferation is present in estrogen-receptor HER2-positive primary breast cancer after neoadjuvant letrozole. J. Clin. Oncol. 24, 3019–3025.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Fox, E. M., Miller, T. W., Balko, J. M., Kuba, M. G., Sanchez, V., Smith, R. A., et al. (2011). A kinome-wide screen identifies the insulin/IGF-I receptor pathway as a mechanism of escape from hormone dependence in breast cancer. Cancer Res. 71, 6773–6784.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Gewinner, C., Wang, Z. C., Richardson, A., Teruya-Feldstein, J., Etemadmoghadam, D., Bowtell, D., et al. (2009). Evidence that inositol polyphosphate 4-phosphatase type II is a tumor suppressor that inhibits PI3K signaling. Cancer Cell 16, 115–125.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Goss, P. E., Ingle, J. N., Martino, S., Robert, N. J., Muss, H. B., Piccart, M. J., et al. (2003). A randomized trial of letrozole in postmenopausal women after five years of tamoxifen therapy for early-stage breast cancer. N. Engl. J. Med. 349, 1793–1802.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Goss, P. E., Ingle, J. N., Pater, J. L., Martino, S., Robert, N. J., Muss, H. B., et al. (2008). Late extended adjuvant treatment with letrozole improves outcome in women with early-stage breast cancer who complete 5 years of tamoxifen. J. Clin. Oncol. 26, 1948–1955.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Howell, A., Cuzick, J., Baum, M., Buzdar, A., Dowsett, M., Forbes, J. F., et al. (2005). Results of the ATAC (Arimidex, Tamoxifen, Alone or in Combination) trial after completion of 5 years’ adjuvant treatment for breast cancer. Lancet 365, 60–62.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Howell, A., Robertson, J. F., Quaresma Albano, J., Aschermannova, A., Mauriac, L., Kleeberg, U. R., et al. (2002). Fulvestrant, formerly ICI 182,780, is as effective as anastrozole in postmenopausal women with advanced breast cancer progressing after prior endocrine treatment. J. Clin. Oncol. 20, 3396–3403.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Howell, A., and Sapunar, F. (2011). Fulvestrant revisited: efficacy and safety of the 500-mg dose. Clin. Breast Cancer 11, 204–210.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Ingle, J. N., Suman, V. J., Rowland, K. M., Mirchandani, D., Bernath, A. M., Camoriano, J. K., et al. (2006). Fulvestrant in women with advanced breast cancer after progression on prior aromatase inhibitor therapy: North Central Cancer Treatment Group Trial N0032. J. Clin. Oncol. 24, 1052–1056.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Maira, S. M., Pecchi, S., Huang, A., Burger, M., Knapp, M., Sterker, D., et al. (2012). Identification and characterization of NVP-BKM120, an orally available pan-class I PI3-kinase inhibitor. Mol. Cancer Ther. 11, 317–328.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Maira, S. M., Stauffer, F., Brueggen, J., Furet, P., Schnell, C., Fritsch, C., et al. (2008). Identification and characterization of NVP-BEZ235, a new orally available dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor with potent in vivo antitumor activity. Mol. Cancer Ther. 7, 1851–1863.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Mamounas, E. P., Jeong, J. H., Wickerham, D. L., Smith, R. E., Ganz, P. A., Land, S. R., et al. (2008). Benefit from exemestane as extended adjuvant therapy after 5 years of adjuvant tamoxifen: intention-to-treat analysis of the National Surgical Adjuvant Breast And Bowel Project B-33 trial. J. Clin. Oncol. 26, 1965–1971.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Mayer, I. A., Abramson, V. G., Balko, J. M., Isakoff, S. J., Kuba, M. G., Sanders, M., et al. (2012). SU2C phase Ib study of pan-PI3K inhibitor BKM120 with letrozole in ER+/HER2- metastatic breast cancer (MBC). J. Clin. Oncol. 30(Suppl.), abstract 510.

Miller, T. W., Balko, J. M., and Arteaga, C. L. (2011a). Phosphatidylinositol 3-kinase and antiestrogen resistance in breast cancer. J. Clin. Oncol. 29, 4452–4461.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Miller, T. W., Balko, J. M., Fox, E. M., Ghazoui, Z., Dunbier, A., Anderson, H., et al. (2011b). ERα-dependent E2F transcription can mediate resistance to estrogen deprivation in human breast cancer. Cancer Discov. 1, 338–351.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Miller, T. W., Forbes, J. T., Shah, C., Wyatt, S. K., Manning, H. C., Olivares, M. G., et al. (2009). Inhibition of mammalian target of rapamycin is required for optimal antitumor effect of HER2 inhibitors against HER2-overexpressing cancer cells. Clin. Cancer Res. 15, 7266–7276.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Miller, T. W., Hennessy, B. T., Gonzalez-Angulo, A. M., Fox, E. M., Mills, G. B., Chen, H., et al. (2010). Hyperactivation of phosphatidylinositol-3 kinase promotes escape from hormone dependence in estrogen receptor-positive human breast cancer. J. Clin. Invest. 120, 2406–2413.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Musgrove, E. A., and Sutherland, R. L. (2009). Biological determinants of endocrine resistance in breast cancer. Nat. Rev. Cancer 9, 631–643.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

O’Reilly, K. E., Rojo, F., She, Q. B., Solit, D., Mills, G. B., Smith, D., et al. (2006). mTOR inhibition induces upstream receptor tyrosine kinase signaling and activates Akt. Cancer Res. 66, 1500–1508.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Osborne, C. K., Pippen, J., Jones, S. E., Parker, L. M., Ellis, M., Come, S., et al. (2002). Double-blind, randomized trial comparing the efficacy and tolerability of fulvestrant versus anastrozole in postmenopausal women with advanced breast cancer progressing on prior endocrine therapy: results of a North American trial. J. Clin. Oncol. 20, 3386–3395.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Perey, L., Paridaens, R., Hawle, H., Zaman, K., Nole, F., Wildiers, H., et al. (2007). Clinical benefit of fulvestrant in postmenopausal women with advanced breast cancer and primary or acquired resistance to aromatase inhibitors: final results of phase II Swiss Group for Clinical Cancer Research Trial (SAKK 21/00). Ann. Oncol. 18, 64–69.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Regan, M. M., Neven, P., Giobbie-Hurder, A., Goldhirsch, A., Ejlertsen, B., Mauriac, L., et al. (2011). Assessment of letrozole and tamoxifen alone and in sequence for postmenopausal women with steroid hormone receptor-positive breast cancer: the BIG 1-98 randomised clinical trial at 8.1 years median follow-up. Lancet Oncol. 12, 1101–1108.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Robertson, J. F., Llombart-Cussac, A., Rolski, J., Feltl, D., Dewar, J., Macpherson, E., et al. (2009a). Activity of fulvestrant 500 mg versus anastrozole 1 mg as first-line treatment for advanced breast cancer: results from the FIRST study. J. Clin. Oncol. 27, 4530–4535.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Robertson, J. F. R., Dixon, J. M., Sibbering, D. M., Jahan, A., Ellis, I. O., Channon, E. J., et al. (2009b). Tumor biomarker changes following pre-surgical treatment with 500 mg fulvestrant plus anastrozole versus 500 mg fulvestrant alone and 1 mg anastrozole alone. Cancer Res. 69, abstract 24.

Robertson, J. F., Osborne, C. K., Howell, A., Jones, S. E., Mauriac, L., Ellis, M., et al. (2003). Fulvestrant versus anastrozole for the treatment of advanced breast carcinoma in postmenopausal women: a prospective combined analysis of two multicenter trials. Cancer 98, 229–238.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Sabnis, G., Goloubeva, O., Jelovac, D., Schayowitz, A., and Brodie, A. (2007). Inhibition of the phosphatidylinositol 3-kinase/Akt pathway improves response of long-term estrogen-deprived breast cancer xenografts to antiestrogens. Clin. Cancer Res. 13, 2751–2757.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Sanchez, C. G., Ma, C. X., Crowder, R. J., Guintoli, T., Phommaly, C., Gao, F., et al. (2011). Preclinical modeling of combined phosphatidylinositol-3-kinase inhibition with endocrine therapy for estrogen receptor-positive breast cancer. Breast Cancer Res. 13, R21.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Schuler, W., Sedrani, R., Cottens, S., Haberlin, B., Schulz, M., Schuurman, H. J., et al. (1997). SDZ RAD, a new rapamycin derivative: pharmacological properties in vitro and in vivo. Transplantation 64, 36–42.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Turner, N., Pearson, A., Sharpe, R., Lambros, M., Geyer, F., Lopez-Garcia, M. A., et al. (2010). FGFR1 amplification drives endocrine therapy resistance and is a therapeutic target in breast cancer. Cancer Res. 70, 2085–2094.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Yamnik, R. L., Digilova, A., Davis, D. C., Brodt, Z. N., Murphy, C. J., and Holz, M. K. (2009). S6 kinase 1 regulates estrogen receptor alpha in control of breast cancer cell proliferation. J. Biol. Chem. 284, 6361–6369.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Zhang, Y., Moerkens, M., Ramaiahgari, S., De Bont, H., Price, L., Meerman, J., et al. (2011). Elevated insulin-like growth factor 1 receptor signaling induces antiestrogen resistance through the MAPK/ERK and PI3K/Akt signaling routes. Breast Cancer Res. 13, R52.

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Keywords: PI3K, breast cancer, antiestrogen, aromatase, fulvestrant, tamoxifen, estrogen receptor

Citation: Fox EM, Arteaga CL and Miller TW (2012) Abrogating endocrine resistance by targeting ERα and PI3K in breast cancer. Front. Oncol. 2:145. doi: 10.3389/fonc.2012.00145

Received: 21 August 2012; Paper pending published: 18 September 2012;
Accepted: 30 September 2012; Published online: 16 October 2012.

Edited by:

Alexandre Arcaro, University of Bern, Switzerland

Reviewed by:

Keisuke Ito, Harvard Medical School, USA
Clement Lee, Mount Sinai School of Medicine, USA

Copyright: © 2012 Fox, Arteaga and Miller. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

*Correspondence: Todd W. Miller, Dartmouth–Hitchcock Medical Center, One Medical Center Drive, HB-7936, Lebanon, NH 03756, USA. e-mail: todd.w.miller@dartmouth.edu