Original Research ARTICLE

Front. Physiol., 12 December 2012 | doi: 10.3389/fphys.2012.00465

The effects of thermal acclimation on lethal temperatures and critical thermal limits in the green vegetable bug, Nezara viridula (L.) (Hemiptera: Pentatomidae)

Pol Chanthy1,2, Robert J. Martin3,4, Robin V. Gunning5 and Nigel R. Andrew1*
  • 1Centre for Behavioural and Physiological Ecology, Department of Zoology, School of Environmental and Rural Sciences, Faculty of Arts and Sciences, University of New England, Armidale, NSW, Australia
  • 2Cambodian Agricultural Research and Development Institute, Phnom Penh, Cambodia
  • 3Agricultural Systems Research Cambodia Co. Ltd., Battambang, Cambodia
  • 4Maddox Jolie-Pitt Foundation, Rotanak Commune, Battambang, Cambodia
  • 5NSW Department of Primary Industries, Tamworth Agricultural Institute, Calala, NSW, Australia

According to geographical distribution, Nezara viridula (Heteroptera: Pentatomidae) can be found across tropical, subtropical, and temperate regions and this pattern is assumed to reflect differences in thermal adaptation, particularly in cold tolerance. Here the lethal temperature (LT) and critical thermal limits (CTL) (thermal tolerance) are examined for N. viridula. The upper LT for N. viridula at two contrasting climate locations (Breeza and Grafton, New South Wales, Australia) was 40.3°C with 20% survival under the stress of high temperature. The lower LT did not differ between these two populations and was −8.0°C with 20% survival under low temperature stress. Survival of N. viridula increased after acclimation at high temperature for 7 days. In contrast, when acclimated at lower temperatures (10 and 15°C), survival of Breeza and Grafton N. viridula was lower than 20% at −8.0°C. Control-reared N. viridula adults (25°C) had a mean CTMinOnset (cold stupor) of 1.3 ± 2.1°C and a mean CTMax (heat coma) of 45.9 ± 0.9°C. After 7 days of acclimation at 10, 20, 30, or 35°C, N. viridula adults exhibited a 1°C change in CTMax and a ~1.5°C change in CTMinOnset. CTMax and CTMinOnset of Breeza and Grafton N. viridula populations did not differ across acclimation temperatures. These results suggest that short-term temperature acclimation is more important than provenance for determining LTs and CTL in N. viridula.

Keywords: Nezara viridula, thermal tolerance, lethal temperature, critical thermal limits, acclimation temperature

Citation: Chanthy P, Martin RJ, Gunning RV and Andrew NR (2012) The effects of thermal acclimation on lethal temperatures and critical thermal limits in the green vegetable bug, Nezara viridula (L.) (Hemiptera: Pentatomidae). Front. Physio. 3:465. doi: 10.3389/fphys.2012.00465

Received: 26 October 2012; Accepted: 24 November 2012;
Published online: 12 December 2012.

Edited by:

Audrey Dussutour, Centre National de la Recherche Scientifique, France

Reviewed by:

Ulrich Theopold, Stockholm University, Sweden
David Rivers, Loyola University Maryland, USA

Copyright © 2012 Chanthy, Martin, Gunning and Andrew. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

*Correspondence: Nigel R. Andrew, Centre for Behavioural and Physiological Ecology, Department of Zoology, School of Environmental and Rural Sciences, Faculty of Arts and Sciences, University of New England, Armidale, NSW 2351, Australia. e-mail: nandrew@une.edu.au

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