Modulation of the Plasma Kallikrein-Kinin System Proteins Performed by Heparan Sulfate Proteoglycans

Human plasma kallikrein-kinin system proteins are related to inflammation through bradykinin. In the proximity of its target cells, high molecular weight kininogen (H-kininogen) is the substrate of plasma kallikrein, which releases bradykinin from H-kininogen. Heparan sulfate proteoglycans (HSPGs) play a critical role in either recruiting kinin precursors from the plasma, or in the assembly of kallikrein-kinin system components on the cell surface. Furthermore, HSPGs mediate the endocytosis and activation of H-kininogen and plasma prekallikrein. In the presence of HSPGs (Chinese hamster ovary cell, CHO-K1, wild type cells) both heparin and heparan sulfate strongly inhibit the H-kininogen interaction with the cell membrane. H-kininogen is internalized in endosomal acidic vesicles in CHO-K1 but not in CHO-745 cells (mutant cells deficient in glycosaminoglycan biosynthesis). The endocytosis process is lipid raft-mediated and is dependent on caveolae. Both types of CHO cells do not internalize bradykinin-free H-kininogen. At pH 7.35, bradykinin is released from H-kininogen on the surface of CHO-745 cells only by serine proteases; however, in CHO-K1 cells either serine or cysteine proteases are found to be involved. The CHO-K1 cell lysate contains different kininogenases. Plasma prekallikrein endocytosis in CHO-K1 cells is independent of H-kininogen, and also prekallikrein is not internalized by CHO-745 cells. Plasma prekallikrein cleavage/activation is independent of glycosaminoglycans but plasma kallikrein formation is more specific on H-kininogen assembled on the cell surface through glycosaminoglycans. In this mini-review, the importance of HSPGs in the regulation of plasma kallikrein-kinin system proteins is shown.

Plasma Kallikrein-Kinin System

In humans, the physiological plasma kallikrein-kinin system (KKS) pathway is composed of high molecular weight kininogen (H-kininogen), plasma pre (kallikrein) and bradykinin (BK). The KKS pathway is very important for inflammatory processes, through BK activities that are mediated by the constitutive G-protein-coupled receptor BK B2 (B2R) present in different cells (Regoli et al., 1996; Schmaier, 2016).

H-kininogen is a single polypeptide chain (120 kD), characterized as a six domain multifunctional protein playing antithrombotic, profibrinolytic, proinflammatory, andantimicrobial roles, and is an inhibitor of cysteine proteases and regulator of angiogenesis. After cleavage by plasma kallikrein (KAL) at Lys₃₆₂-Arg₃₆₃ and Arg₃₇₁-Ser₃₇₂, the H-kininogen turns BK-free H-kininogen into a two-chain protein composed of an N-terminal heavy chain (containing three domains, D1–D3, 62 kD) and a C-terminal light chain (containing two domains, D5–D6, 61 kD) bound to each other by a single disulfide bond between Cys₁₀-Cys₅₉₆ (Colman and Schmaier, 1997; Lalmanach et al., 2010).

The important peptide BK (Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) was first described by Mauricio Rocha e Silva and co-workers (Rocha e Silva et al., 1949) as a substance that produces a slow delayed contraction of the isolated guinea pig ileum after incubation with either extracts of Bothrops jararaca venom or trypsin with the globulin fraction of dog plasma. BK corresponds to H-kininogen domain 4 (D4) and it reproduces many of the characteristics of an inflammatory state, such as changes in local blood pressure, edema, and pain, resulting in vasodilation and increased microvessel permeability (Bhoola et al., 1992). The release of pro-inflammatory and hyperalgesic mediators such as neuropeptides, leukotrienes, and cytokines, and the activation of sensory nerve terminals are the underlying causes of pro-inflammatory and nociceptive pharmacological effects induced by BK (Heitsch, 2003; Couture et al., 2014).

Plasma prekallikrein (PK) is the zymogen of KAL (EC 3.4.21.34), a serine protease synthesized predominantly in the liver. Its mRNA codes for a single chain protein which, according to the degree of glycosylation in the C-terminal portion, may appear as a doublet (85 and 88 kD) independent of the protein reduction. The classic activation of PK by factor XIIa occurs through cleavage of the Arg₃₇₁-Ile₃₇₂ bond producing KAL, containing an intact N-terminal heavy chain (53 kD) and a C-terminal light chain (33–36 kD) linked via a single disulfide bond between Cys₃₆₄-Cys₄₈₄. The protease domain is in the light chain and contains the catalytic domain (His₄₁₅, Asp₄₆₄, and Ser₅₅₉) (Colman and Schmaier, 1997). PK and H-kininogen circulate as bimolecular complexes and ~75–80% of PK circulates as bound at plasma equilibrium conditions (Kaplan, 2014). The N-terminal heavy chain comprises four apple domains (A1–A4) and the binding affinity sites on PK for H-kininogen are A2>A4 ≈ A1>A3 (Renné et al., 1999). Two forms of active KAL have been described: α-KAL which presents both intact heavy and light chains, and β-KAL in which the heavy chain has undergone a single cleavage to produce fragments of ~18,000 and 28,000 kD, linked by disulfide bridges. Only one cleavage on the β-KAL heavy chain impairs the efficiency of BK release; therefore, the heavy chain does not seem to be necessary for non-specific cleavages of H-kininogen by β-KAL (Motta et al., 1989; Page and Colman, 1991).

KAL plays a central role in a variety of proteolytic systems, such as the intrinsic pathway of coagulation, the KKS, the fibrinolysis pathway, the renin–angiotensin system, and the complement pathways. Therefore, it can be considered an important regulator in the pathogenesis of thrombosis, inflammation, and blood pressure (Kolte and Shariat-Madar, 2016).

Plasma Kallikrein-Kinin System and Cell Surface Interaction

In the intravascular compartment, the interaction and activation of KKS on the cell surface of platelets, neutrophils, endothelial cells, and macrophages have been described (Colman, 2006; Schmaier and McCrae, 2007; Barbasz et al., 2008). The membrane-binding proteins of H-kininogen or BK-free H-kininogen on endothelial cells include the globular domains of complement factor C1q receptor (gC1qR), urokinase plasminogen activator receptor (uPAR) and cytokeratin 1 (CK1), in which the affinity measured using surface plasmon resonance is gC1qR>CK1>soluble uPAR, indicating that gC1qR is dominant for binding (Pixley et al., 2011).

Once assembled on the cell surface H-kininogen functions as a putative receptor for PK; therefore, a lower affinity of PK can be detected in the absence of added H-kininogen; the cell-associated PK is rapidly converted to KAL in the presence of H-kininogen, that is proteolyzed during the KAL formation producing BK, and Motta et al. (1998) first describe the factor XII independent pathway for contact factor activation on human umbilical vein endothelial cells (HUVECs) that regulate BK production.

BK plays an important role in regulating angiogenesis through upregulation of endogenous basic fibroblast growth factor through the inductive G-protein-coupled receptor BK B1 or viral fibroblast growth factor through the B2R, by regulation of vascular permeability or stimulation of cell proliferation through the B2R. The H-kininogen cleavage by KAL promotes conformational changes in BK-free H-kininogen and exposure of domain 5 (D5), which inhibits endothelial cell migration and proliferation, both of which play a role in angiogenesis (Guo and Colman, 2005). This potent anti-angiogenic activity occurs through tight-binding to cell surface tropomyosin which also induces endothelial cell apoptosis (McCrae et al., 2005). The peptide sequence on H-kininogen D5, comprising the residues His₄₉₇–K₅₂₀, is important for homing of both T and B cells to lymph nodes, which is a powerful mechanism of coupling inflammation to adaptive immunity (Ponda and Breslow, 2016).

PK is brought to the endothelial surface by H-kininogen or BK-free H-kininogen since it binds to a peptide sequence (Trp₅₆₉-Lys₅₉₅) on domain 6 (D6) (Vogel et al., 1990). The activation of the KKS on endothelial cells needs both H-kininogen and zinc ions (Røjkjaer et al., 1998). Zinc ions mediate both H-kininogen and BK-free H-kininogen binding with high affinity to endothelial cells (Reddigari et al., 1993) and through domain 5 (D5) both forms also bind with high affinity to heparin, a negatively charged glycosaminoglycan (GAG; Björk et al., 1989).

Heparin interacts with Lys and/or Arg residues within proteins via electrostatic interaction and zinc facilitates binding to heparin by binding to expose His residues and stabilizes the non-covalent complex between heparin and both forms of H-kininogen (Lin et al., 2000). H-kininogen D5 contains two subdomains, a His-Gly-rich region (Lys₄₂₀-Asp₄₇₄) and a His-Gly-Lys-rich region (His₄₇₅-Lys₅₀₂); H-kininogen exhibits a higher affinity to heparin (lower K_D) than BK-free H-kininogen because the former loses a peptide sequence (Ser₃₇₂-Arg₄₁₉) which corresponds to a third cleavage by KAL after BK release from H-kininogen (Nakayasu and Nagasawa, 1979).

Several proteinases from two major classes, the serine and cysteine proteases, cleave H-kininogen; nevertheless, they differ in specificity and not all of them release BK or Lys-BK. Among the serine proteases are KAL, tissue kallikrein, trypsin, factor XIIa, factor XIa, plasmin and neutrophil elastase (Mauron et al., 2000). At inflammatory sites, a combined action of tryptase/neutrophil elastase or KAL/neutrophil elastase may release kinins. Among cysteine proteases are calpains and cathepsin L that release kinins from H-kininogen at slightly acidic pH, suggesting these enzymes as candidates for kinin production in a pathophysiological environment (Lalmanach et al., 2010).

The findings of the current research group show that cathepsin B has kininogenase activity at pH 6.3, which is improved in the absence of divalent cations (Zn²⁺, Mg²⁺, and Ca²⁺), however, at pH 7.35 cathepsin kininogenase activity is impaired suggesting that H-kininogen is a substrate for cathepsin B under pathophysiological conditions (Barros et al., 2004a).

Glycosaminoglycans and Inflammation

Heparan sulfate (HS) polysaccharide chains are ubiquitous GAGs in animal cells (Dietrich et al., 1983). These classes of molecules are heteropolysaccharides composed of repeating units of disaccharides, a uronic acid residue, either D-glucuronic acid or L-iduronic acid, and D-glucosamine with N- and 6-O-sulfates and N-acetyl substitutions (Dietrich et al., 1998). HS occurs at the cell surface and in the extracellular matrix (ECM) as proteoglycans (PGs). Most of the cellular HS derives from the syndecans and glypicans PGs. The syndecan family is associated with the cell membranes via transmembrane core proteins (Yanagishita and Hascall, 1992; Elenius and Jalkanen, 1994), and the glypican family is anchored by glycosilyl phosphatidylinositol-anchor core proteins (David, 1993). In addition, heparan sulfate proteoglycans (HSPGs) are present in basement membranes as the perlecan family (Iozzo et al., 1994).

HSPGs regulate a wide variety of biological process including hemostasis, inflammation, angiogenesis, growth factors, and cell adhesion, and play a major role in the ECM (Conrad, 1998). The interactions occurring in the ECM with HSPG have shown the direct regulation of the fibroblast growth factors (FGFs) diffusion that can determine the shape of growth concentration gradients in development, as well as the storage and release of FGFs in tissue homeostasis. The growth factor/morphogen-type signals generated by FGFs require the assembly of the ternary complex of FGF ligand, FGF receptor (FGFR) and HS, which engages both the ligand and receptor, acting as a co-receptor (Li et al., 2016). The heparin-related GAG chains of the HSPGs are involved in the inflammatory process through binding and modification of the activity of several molecules (Götte, 2003).

Heparin and HSPGs can alter the proteolytic activity of various serine proteases of the coagulation cascade as well as their natural inhibitors, the serpins (Ermolieff et al., 1994; Gettins et al., 1996; Fath et al., 1998). HSPGs can also control the activity of several serine proteases directly involved in the inflammatory response. Data from the literature show that ectodomains of syndecan-1 and syndecan-4 present in acute inflammatory fluid are able to regulate the proteolytic activity of cathepsin G and elastase. The enzymatic degradation of polysaccharide chains of HS in HSPGs promotes strong inactivation of elastase and cathepsin G serine proteinases present in the inflammatory fluid (Kainulainen et al., 1998). The syndecan-1 ectodomain protects the inhibition of cathepsin G by the natural inhibitors, the serpin α1-antichymotrypsin and squamous cell carcinoma antigen-2 and protects the inhibition of elastase by α1-antichymotrypsin.

At the neutrophil surface HSPGs bind human neutrophil elastase (HNE) and preserve its activity by protecting it from inhibition by α1-antitrypsin and secretory leukocyte peptidase inhibitor and focus the activity of HNE to the pericellular environment (Campbell and Owen, 2007). Syndecan knockout mice show deficits in tissue repair (Bishop et al., 2007). In mast cells, intracellular heparin PG serglycin compartmentalizes various proteolytic enzymes, among them chymase and tryptase proteases (Pejler et al., 2007). The major intracellular PG of hematopoietic cells is serglycin PG which has been related to sorting and packing of granule HNE (Niemann et al., 2007).

HNE can also regulate tissue inhibitor of metalloproteases-1 (TIMP-1) and metalloproteinase-9 (MMP-9) activities. In the pro-MMP-9/TIMP-1 complex, HNE preferentially inactivates TIMP-1 and renders pro-MMP-9 activity through MMP-3 (Itoh and Nagase, 1995). TIMP-1, as an inhibitor of MMP-2 and MMP-9, is tightly correlated to the maintenance of the ECM structure (Murphy and Nagase, 2008). An MMP-9/TIMP-1 imbalance, caused by the proteolytic activity of HNE, has an important pathophysiological role in the sputum of patients with cystic fibrosis (Jackson et al., 2010), intracranial hemorrhage (Alvarez-Sabín et al., 2004), abdominal aortic aneurysm (Wiernicki et al., 2010) and bone resorption (Bord et al., 1997).

Heparin increases eight-fold the initial rate of pro-MMP-9 autolytic activation (Crabbe et al., 1993). Interestingly, the major clinical complication of heparin anticoagulant therapy is hemorrhage (Zidane et al., 2000) and osteoporosis by increasing bone resorption (Rajgopal et al., 2008) as a side effect. Heparin accelerates the rate of hydrolysis of TIMP-1 by HNE and the most important adverse effects of heparin therapy are related to excessive activation of MMP-9 (Nunes et al., 2011).

Human neutrophil proteases can release Met-Lys-BK and BK from low-(L) and H-kininogens (Stuardo et al., 2004). The cooperative action of mast cell tryptase and HNE can release BK from H-kininogen and L-kininogen, with efficiency not much lower than that of KAL. Interestingly, the release of BK from oxidized kininogen substrate by the tryptase/elastase mixture was not altered (Kozik et al., 1998). Therefore, the plasma level of BK is higher in wild-type C57BL/6 mice than in elastase-deficient mice (Sahoo et al., 2014). It is important to note that both tryptase (Hallgren et al., 2005; Pejler et al., 2007) and neutrophil elastase proteases (Campbell and Owen, 2007; Niemann et al., 2007) have their proteolytic activity controlled by heparin and HSPGs.

Heparan Sulfate Proteoglycans and the Plasma Kallikrein-Kinin System

In the early 2000s, not only HS but also chondroitin sulfate (CS) GAGs were indicated as putative receptors that accumulate H-kininogen on the cell surface of HUVECs and other cells in a zinc-dependent manner, along with H-kininogen binding proteins, the integrin receptor Mac-1/α_Mβ₂, p33/gC1qR, uPAR, CK1, thrombospondin-1 and glycoprotein-Ib (Renné et al., 2000; Renné and Müller-Esterl, 2001; Fernando et al., 2003).

Previous studies concerning KKS purified protein assembly on the ECM of different endothelial cell lines, from rabbit aorta endothelial cells (RAECs) and ECV304 cells, suggest that not only the cell membranes but also the ECM produced by these cells assemble H-kininogen/PK as a complex and form KAL that activates pro-urokinase, which in turn activates plasminogen (Motta et al., 2001).

On the RAEC surface or ECM, GAGs also influence this process because in the presence of Zn²⁺ heparin abolishes H-kininogen binding which is reduced by HS and other GAGs; in contrast, only heparin reduces H-kininogen binding to the ECV304 surface or ECM. Heparin does not modify PK binding and activation in the presence of H-kininogen assembled to the cell surface or ECM. In the fluid phase, heparin in the presence of Zn²⁺augments the BK release from H-kininogen after hydrolysis by KAL. GAGs that accumulate in inflammatory fluids or are used as a therapeutic drug (e.g., heparin) could act as pro- or anti-inflammatory mediators depending on different factors within the cell environment (Gozzo et al., 2011).

The current research group is the first to show that the interaction of H-kininogen and PK on the cell surface mediated by HSPGs results in endocytosis (Melo et al., 2009; Veronez et al., 2014; Damasceno et al., 2015; Figure 1). H-kininogen interacts with cellular sites in either RAECs or epithelial CHO cells, wild type CHO-K1 cells and CHO-745 cells, mutant cells deficient in xylosyltransferase and as consequence is involved in GAG biosynthesis. The HS chain of HSPG is involved in H-kininogen binding to RAECs and CHO-K1 cells. The interaction parameters of H-kininogen with RAECs and CHO cells are strongly dependent on temperature and the interaction between H-kininogen and cells was not totally reversible at 37°C; the interaction with CHO cells is related to vital processes also influenced by ATP synthesis; the H-kininogen interaction in CHO cells is independent of cycloheximide, indicating that new protein synthesis does not influence H-kininogen binding. GAGs are not the main cellular receptors; however, GAGs modulate the cellular receptors of H-kininogen in CHO cells (Melo et al., 2009).

FIGURE 1

Figure 1. Modulation of Plasma Kallikrein-Kinin System: the Role of Heparan Sulfate Proteoglycans. H-kininogen (HK) or plasma prekallikrein (PK) alone, or as a complex (HK/PK), are associated with the cell surface. The heparan sulfate proteoglycans (HSPG) in the membrane microdomains can function as ligands for these molecules, promoting caveolae-mediated endocytosis. Once present in the primary endosome, these ligands can escape to the cytoplasm or be processed after the fusion between endosomes and lysosomes. Furthermore, these molecules may undergo proteolysis on the cell surface by serine or cysteine proteases. The HSPGs can be recycled to the cell surface.

In CHO-K1 cells, after H-kininogen interacts with HS at the cell surface, it is internalized by endocytosis followed by a fusion step within an acidic endosomal compartment. The endocytosis process of H-kininogenin CHO-K1 cells is disrupted by pretreatment of the cells using chloroquine, an alkalinizing agent of acid endosomes; cholesterol depletion by methyl-β-cyclodextrin also inhibits endocytosis; the endocytosis of H-kininogen is independent of transferrin endocytosis, strongly indicating that the endocytosis of H-kininogen is not dependent on clathrin. The membrane lipid raft domains/caveolae mediate the endocytotic process of H-kininogen, as the colocalization of H-kininogen with caveolin-1 has been demonstrated (Melo et al., 2009; Damasceno et al., 2015).

In contrast, CHO-745 cells, which are almost completely devoid of GAGs, do not take up intact H-kininogen; the inhibition of GAG sulfation blocks the endocytosis process, and the integrity of H-kininogen is very important for internalization since BK-free H-kininogen does not become internalized (Veronez et al., 2014; Damasceno et al., 2015).

The cysteine proteases play a role in H-kininogen processing on the cell surface and in acidic endosomal vesicles. At neutral pH and after H-kininogen assembly on the cell surface, kinin generation was much higher in CHO-K1 cells, and tumorigenic cells, when compared with RAECs, as expected, as either serine or cysteine proteases are involved in kinin release. In CHO-745 cells, only serine protease activity is detected consistently with the regulation of cysteine proteases by GAGs/PGs in H-kininogen processing and kinin release (Melo et al., 2009; Damasceno et al., 2015). The lysate fraction of CHO-K1 cells possesses kininogenase activity at pH7.4 but the total cleavage of intact H-kininogen is more effective at pH 5.5 after a 4 h incubation. In crude lysates, the hydrolysis patterns are quite similar suggesting a kininogenase activity at a pH optimum of 5.5 that can still work at pH 7.4, albeit more slowly. The antipain-Sepharose affinity chromatography of the CHO-K1 cell lysate fraction successfully separates different kininogenases (Damasceno et al., 2015).

Other groups have shown that H-kininogen binding to HS and CS efficiently interferes with BK release by KAL, in plasma and on endothelial surfaces (Renné et al., 2005), and two enzymes purified from HUVECs are described as PK activators on cells, the heat shock protein 90 and prolylcarboxypeptidase (Bryant and Shariat-Madar, 2009).

PK interacts directly with the endothelial cell surface independent of the presence of H-kininogen (Motta et al., 1998). The PK interaction with CHO and ECV304 cells shows that although the process is temperature-dependent, it also depends on the GAG composition of the cell type. HSPGs direct PK to acidic endosomal vesicles and the endocytosis process of PK/KAL depends on GAGs, but not those bound to H-kininogen. PK interaction with GAGs does not disturb its cleavage/activation; therefore, H-kininogen bound to GAGs may modulate PK as a control mechanism of KAL activity (Veronez et al., 2014). Either cathepsin B or cathepsin L, which are lysosomal cysteine proteases, might hydrolyze PK or KAL (Barros et al., 2004b).

Figure 1 illustrates the concept that after blood vessel injury, H-kininogen and PK or KAL can interact and bind to the HSPGs of non-endothelial cells. On lipid raft domains/caveolae or endosomes, HSPGs mediate the endocytosis of intact H-kininogen, which can be processed by different classes of proteases. The cleavage produces H-kininogen fragments that may play roles as cystatins, effectors of innate immunity, inflammation, angiogenesis, and coagulation. The KAL proteolytic balance of H-kininogen/BK free H-kininogen forms is controlled by GAGs, which may promote this control in two different ways: controlling the proteolytic activity of the ternary complex GAG/H-kininogen/KAL or the cellular compartmentalization of KKS proteins via endocytosis.

Author Contributions

GM and IT conceived, designed and wrote the review. GM has experience in plasma kallikrein/kinin system and IT has experience in proteoglycans field. Both authors are professors at Department of Biochemistry at Medical School (Escola Paulista de Medicina) which has very important tradition and contribution in both fields. Both authors work also on cell biology and enzymes.

Funding

The authors acknowledge financial support from FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP#15/03964-6; FAPESP#16/14827-2), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior).

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The reviewer CO and handling Editor declared their shared affiliation, and the handling Editor states that the process nevertheless met the standards of a fair and objective review.

Acknowledgments

The authors acknowledge the graphic art made by Cleide Oliveira, Ph.D. (Universidade Brasil).

Abbreviations

BK, bradykinin; CHO, cell line from Chinese hamster ovary cells; CK1, cytokeratin 1; CS, chondroitin sulfate; ECM, extracellular matrix; ECV304, cell line from spontaneously transformed human umbilical vein endothelial cells; gC1qR, globular domains of complement factor C1q receptor; GAG, glycosaminoglycan; B2R, G-protein-coupled receptor bradykinin B2; H-kininogen, high molecular weight kininogen; HNE, human neutrophil elastase; HUVECs, human umbilical vein endothelial cells; HS, heparan sulfate; HSPGs, heparan sulfate proteoglycans; KKS, plasma kallikrein-kinin system; KAL, plasma kallikrein; MMP-9, metalloproteinase-9; PGs, proteoglycans; PK, plasma prekallikrein; RAECs, rabbit aorta endothelial cell line; TIMP-1, tissue inhibitor of metalloproteases-1.

References

Alvarez-Sabín, J., Delgado, P., Abilleira, S., Molina, C. A., Arenillas, J., Ribo, M., et al. (2004). Temporal profile of matrix metalloproteinases and their inhibitors after spontaneous intracerebral hemorrhage: relationship to clinical and radiological outcome. Stroke 35, 1316–1322. doi: 10.1161/01.STR.0000126827.69286.90

PubMed Abstract | CrossRef Full Text | Google Scholar

Barbasz, A., Guevara-Lora, I., Rapala-Kozik, M., and Kozik, A. (2008). Kininogenbinding to the surfaces of macrophages. Int. Immunopharmacol. 8, 211–216. doi: 10.1016/j.intimp.2007.08.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Barros, N. M. T., Puzer, L., Tersariol, I. L. S., Oliva, M. L. V., Sampaio, C. A. M., Carmona, A. K., et al. (2004b). Plasma prekallikrein/kallikrein processing by lysosomal cysteine proteases. Biol. Chem. 385, 1087–1091. doi: 10.1515/BC.2004.141

PubMed Abstract | CrossRef Full Text | Google Scholar

Barros, N. M., Tersariol, I. L., Oliva, M. L., Araújo, M. S., Sampaio, C. A., Juliano, L., et al. (2004a). High molecular weight kininogen as substrate for cathepsin B. Biol. Chem. 385, 551–555. doi: 10.1515/BC.2004.066

PubMed Abstract | CrossRef Full Text | Google Scholar

Bhoola, K. D., Figueroa, C. D., and Worthy, K. (1992). Bioregulation of Kinins: Kallikreins, Kininogens, and Kininases. Pharmacol. Rev. 44, 1–80.

PubMed Abstract | Google Scholar

Bishop, J. R., Schuksz, M., and Esko, J. D. (2007). Heparan sulphate proteoglycans fine-tune mammalian physiology. Nature 446, 1030–1037. doi: 10.1038/nature05817

PubMed Abstract | CrossRef Full Text | Google Scholar

Björk, I., Olson, S. T., Sheffer, R. G., and andShore, J. D. (1989). Binding of heparin to human high molecular weight kininogen. Biochemistry 28, 1213–1221. doi: 10.1021/bi00429a039

PubMed Abstract | CrossRef Full Text | Google Scholar

Bord, S., Horner, A., Hembry, R. M., Reynolds, J. J., and Compston, J. E. (1997). Distribution of matrix metalloproteinases and their inhibitor, TIMP-1, in developing human osteophytic bone. J. Anat. 191, 39–48. doi: 10.1046/j.1469-7580.1997.19110039.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Bryant, J. W., and Shariat-Madar, Z. (2009). Human plasma kallikrein-kinin system: physiological and biochemical parameters. Cardiovasc. Hematol. Agents Med. Chem. 7, 234–250. doi: 10.2174/187152509789105444

PubMed Abstract | CrossRef Full Text | Google Scholar

Campbell, E. J., and Owen, C. A. (2007). The sulfate groups of chondroitin sulfate- and heparan sulfate-containing proteoglycans in neutrophil plasma membranes are novel binding sites for human leukocyte elastase and cathepsin G. J. Biol. Chem. 282, 14645–14654. doi: 10.1074/jbc.M608346200

PubMed Abstract | CrossRef Full Text | Google Scholar

Colman, R. W. (2006). Regulation of angiogenesis by the kallikrein-kinin system. Curr. Pharm. Des. 12, 2599–2607. doi: 10.2174/138161206777698710

PubMed Abstract | CrossRef Full Text | Google Scholar

Colman, R. W., and Schmaier, A. H. (1997). Contact system: a vascular biology modulator with anticoagulant, profibrinolytic, antiadhesive, and proinflammatory attributes. Blood 90, 3819–3843.

PubMed Abstract | Google Scholar

Conrad, H. E. (1998). Heparin-Binding Proteins. New York, NY: Academic Press Inc.

Google Scholar

Couture, R., Blaes, N., and Girolami, J. P. (2014). Kinin receptors in vascular biology and pathology. Curr. Vasc. Pharmacol. 12, 223–248. doi: 10.2174/1570161112666140226121627

PubMed Abstract | CrossRef Full Text | Google Scholar

Crabbe, T., Ionnou, C., and Docherty, A. J. P. (1993). Human progelatinase A can be activated by autolysis at a rate that is concentration-dependent and enhanced by heparin bound to the C-terminal domain. Eur. J. Biochem. 218, 431–438. doi: 10.1111/j.1432-1033.1993.tb18393.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Damasceno, I. Z., Melo, K. R., Nascimento, F. D., Souza, D. S., Araujo, M. S., Souza, S. E., et al. (2015). Bradykinin release avoids high molecular weight kininogen endocytosis. PLoS ONE 10:e0121721. doi: 10.1371/journal.pone.0121721

PubMed Abstract | CrossRef Full Text | Google Scholar

David, G. (1993). Integral membrane heparan sulfate proteoglycans. FASEB J. 7, 1023–1030.

PubMed Abstract | Google Scholar

Dietrich, C. P., Nader, H. B., and Straus, A. H. (1983). Structural differences of heparan sulfates according to the tissue and species of origin. Biochem. Biophys. Res. Commun. 111, 865–871. doi: 10.1016/0006-291X(83)91379-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Dietrich, C. P., Tersariol, I. L. S., Toma, L., Moraes, C. T., Porcionatto, M. A., Oliveira, F. W., et al. (1998). Structure of heparan sulfate: identification of variable and constant oligosaccharide domains in eight heparan sulfates of different origins. Cell. Mol. Biol. 44, 417–429.

PubMed Abstract | Google Scholar

Elenius, K., and Jalkanen, M. (1994). Functions of syndecans—a family of cell surface proteoglycans. J. Cell. Sci. 107, 2975–2982.

Google Scholar

Ermolieff, J., Boudier, C., Laine, A., Meyer, B., and Bieth, J. G. (1994). Heparin protects cathepsin G against inhibition by protein proteinase inhibitors. J. Biol. Chem. 269, 29502–29508.

PubMed Abstract | Google Scholar

Fath, M. A., Wu, X., Hileman, R. E., Linhardt, R. J., Kashem, M. A., Nelson, R. M., et al. (1998). Interaction of secretory leukocyte protease inhibitor with heparin inhibits proteases involved in asthma. J. Biol. Chem. 273, 13563–13569. doi: 10.1074/jbc.273.22.13563

PubMed Abstract | CrossRef Full Text | Google Scholar

Fernando, L. P., Fernando, A. N., Joseph, K., and Kaplan, A. P. (2003). Assessment of the role of heparan sulfate in high molecular weight kininogen binding to human umbilical vein endothelial cells. J. Thromb. Haemost. 1, 2444–2449. doi: 10.1046/j.1538-7836.2003.00438.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Gettins, P. G. W., Patston, P. A., and Olson, S. T. (1996). Serpins: Structure, Function and Biology. Austin, TX: R.G. Landes Co.

Götte, M. (2003). Syndecans in inflammation. FASEB J. 17, 575–591. doi: 10.1096/fj.02-0739rev

PubMed Abstract | CrossRef Full Text | Google Scholar

Gozzo, A. J., Motta, G., Cruz-Silva, I., Nunes, V. A., Barros, N. M. T., Carmona, A. K., et al. (2011). Heparin affects the interaction of kininogen on endothelial cells. Biochimie 93, 1839–1845. doi: 10.1016/j.biochi.2011.07.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Guo, Y. L., and Colman, R. W. (2005). Two faces of high-molecular-weight kininogen (HK) in angiogenesis: bradykinin turns it on and cleaved HK (HKa) turns it off. J. Thromb. Haemost. 3, 670–676. doi: 10.1111/j.1538-7836.2005.01218.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Hallgren, J., Lindahl, S., and Pejler, G. (2005). Structural requirements and mechanism for heparin-dependent activation and tetramerization of human betaI- and betaII-tryptase. J. Mol. Biol. 345, 129–139. doi: 10.1016/j.jmb.2004.10.029

PubMed Abstract | CrossRef Full Text | Google Scholar

Heitsch, H. (2003). The therapeutic potential of bradykinin B2 receptor agonists in the treatment of cardiovascular disease. Expert. Opin. Investig. Drugs 12, 759–770. doi: 10.1517/13543784.12.5.759

PubMed Abstract | CrossRef Full Text | Google Scholar

Iozzo, R., Cohen, I. R., Grässel, S., and Murdock, A. D. (1994). The biology of perlecan: the multifaceted heparan sulphate proteoglycan of basement membranes and pericellular matrices. Biochem. J. 302, 625–639. doi: 10.1042/bj3020625

PubMed Abstract | CrossRef Full Text | Google Scholar

Itoh, Y., and Nagase, H. (1995). Preferential inactivation of tissue inhibitor of metalloproteinases-1 that is bound to the precursor of matrix metalloproteinase-9 (progelatinase B) by human neutrophil elastase. J. Biol. Chem. 270, 16518–16521. doi: 10.1074/jbc.270.28.16518

PubMed Abstract | CrossRef Full Text | Google Scholar

Jackson, P. L., Xu, X., Wilson, L., Weathington, N. M., Clancy, J. P., Blalock, J. E., et al. (2010). Human neutrophil elastase-mediated cleavage sites of MMP-9 and TIMP-1: implications to cystic fibrosis proteolytic dysfunction. Mol. Med. 16, 159–166. doi: 10.2119/molmed.2009.00109

PubMed Abstract | CrossRef Full Text | Google Scholar

Kainulainen, V., Wang, H., Schick, C., and Bernfield, M. (1998). Syndecans, heparan sulfate proteoglycans, maintain the proteolytic balance of acute wound fluids. J. Biol. Chem. 273, 11563–11569. doi: 10.1074/jbc.273.19.11563

PubMed Abstract | CrossRef Full Text | Google Scholar

Kaplan, A. P. (2014). The bradykinin-forming cascade: a historical perspective. Chem. Immunol. Allergy 100, 205–213. doi: 10.1159/000358739

PubMed Abstract | CrossRef Full Text | Google Scholar

Kolte, D., and Shariat-Madar, Z. (2016). Plasma Kallikrein Inhibitors in Cardiovascular Disease: an innovative therapeutic approach. Cardiol. Rev. 24, 99–109. doi: 10.1097/crd.0000000000000069

PubMed Abstract | CrossRef Full Text | Google Scholar

Kozik, A., Moore, R. B., Potempa, J., Imamura, T., Rapala-Kozik, M., and Travis, J. (1998). A novel mechanism for bradykinin production at inflammatory sites. Diverse effects of a mixture of neutrophil elastase and mast cell tryptase versus tissue and plasma kallikreins on native and oxidized kininogens. J. Biol. Chem. 273, 33224–33229. doi: 10.1074/jbc.273.50.33224

PubMed Abstract | CrossRef Full Text | Google Scholar

Lalmanach, G., Naudin, C., Lecaille, F., and Fritz, H. (2010). Kininogens: more than cysteine protease inhibitors and kinin precursors. Biochimie 92, 1568–1579. doi: 10.1016/j.biochi.2010.03.011

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, Y., Sun, C., Yates, E. A., Jiang, C., Wilkinson, M. C., and Fernig, D. G. (2016). Heparin binding preference and structures in the fibroblast growth factor family parallel their evolutionary diversification. Open Biol. 6:150275. doi: 10.1098/rsob.150275

PubMed Abstract | CrossRef Full Text | Google Scholar

Lin, Y., Pixley, R. A., and Colman, R. W. (2000). Kinetic analysis of the role of zinc in the interaction of domain 5 of high-molecular weight kininogen (HK) with heparin. Biochemistry 39, 5104–5110. doi: 10.1021/bi992048z

PubMed Abstract | CrossRef Full Text | Google Scholar

Mauron, T., Lämmle, B., and Wuillemin, W. A. (2000). High molecular weight kininogen is cleaved by FXIa at three sites: Arg409-Arg410, Lys502-Thr503 and Lys325-Lys326. Thromb. Haemost. 83, 709–714.

PubMed Abstract | Google Scholar

McCrae, K. R., Do-ate, F., Merkulo, S., Sun, D., Qi, X., and Shaw, D. E. (2005). Inhibition of angiogenesis by cleaved high molecular weight kininogen (HKa) and HKa domain 5. Curr. Cancer Drug Targets 5, 519–528. doi: 10.2174/156800905774574039

PubMed Abstract | CrossRef Full Text | Google Scholar

Melo, K. R., Gutierrez, A., Nascimento, F. D., Araujo, M. S., Sampaio, M. U., Carmona, A. K., et al. (2009). Involvement of heparan sulfate proteoglycans in cellular uptake of high molecular weight kininogen. Biol. Chem. 390, 145–155. doi: 10.1515/BC.2009.016

PubMed Abstract | CrossRef Full Text | Google Scholar

Motta, G., Rojkjaer, R., Hasan, A. A. K., Cines, D. B., and Schmaier, A. H. (1998). High molecular weight kininogen regulates prekallikrein assembly and activation on endothelial cells: a novel mechanism for contact activation. Blood 91, 516–528.

PubMed Abstract | Google Scholar

Motta, G., Sampaio, M. U., and Sampaio, C. A. (1989). Hydrolysis of synthetic peptides and natural substrates by plasma kallikrein and its light chain. Adv. Exp. Med. Biol. 247B, 239–242. doi: 10.1007/978-1-4615-9546-5_39

PubMed Abstract | CrossRef Full Text | Google Scholar

Motta, G., Shariat-Madar, Z., Mahdi, F., Sampaio, C. A. M., and Schmaier, A. H. (2001). Assembly of high molecular weight kininogen and activation of pr ekallikrein on cell matrix. Thromb. Hemost. 86, 840–847.

Google Scholar

Murphy, G., and Nagase, H. (2008). Progress in matrix metalloproteinase research. Mol. Aspects Med. 29, 290–308. doi: 10.1016/j.mam.2008.05.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Nakayasu, T., and Nagasawa, S. (1979). Studies on human kininogens. I. Isolation, characterization, and cleavage by plasma kallikrein of high molecular weight (HMW)-kininogen. J. Biochem. 85, 249–258. doi: 10.1093/oxfordjournals.jbchem.a132318

PubMed Abstract | CrossRef Full Text | Google Scholar

Niemann, C. U., Åbrink, M., Pejler, G., Fischer, R. L., and Christensen, E. I., Knight, S. D., et al. (2007). Neutrophil elastase depends on serglycin proteoglycan for localization in granules. Blood 109, 4478–4486. doi: 10.1182/blood-2006-02-001719

PubMed Abstract | CrossRef Full Text | Google Scholar

Nunes, G. L., Simões, A., Dyszy, F. H., Shida, C. S., Juliano, M. A., Juliano, L., et al. (2011). Mechanism of heparin acceleration of tissue inhibitor of metalloproteases-1 (TIMP-1) degradation by the human neutrophil elastase. PLoS ONE 6:e21525. doi: 10.1371/journal.pone.0021525

PubMed Abstract | CrossRef Full Text | Google Scholar

Page, J. D., and andColman, R. W. (1991). Localization of distinct functional domains on prekallikrein for interaction with both high molecular weight kininogen and activated factor XII in a 28-kDa fragment (amino acids 141-371). J. Biol. Chem. 266, 8143–8148.

PubMed Abstract | Google Scholar

Pejler, G., Abrink, M., Ringvall, M., and Wernersson, S. (2007). Mast cell proteases. Adv. Immunol. 95, 167–255. doi: 10.1016/S0065-2776(07)95006-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Pixley, R. A., Espinola, R. G., Ghebrehiwet, B., Joseph, K., Kao, A., Bdeir, K., et al. (2011). Interaction of high-molecular-weight kininogen with endothelial cell binding proteins suPAR, gC1qR and cytokeratin 1 determined by surface plasmon resonance (BiaCore). Thromb. Haemost. 105, 1053–1059. doi: 10.1160/TH10-09-0591

PubMed Abstract | CrossRef Full Text | Google Scholar

Ponda, M. P., and Breslow, J. L. (2016). Serum stimulation of CCR7 chemotaxis due to coagulation factor XIIa-dependent production of high-molecular-weight kininogen domain 5. Proc. Natl. Acad. Sci. U.S.A. 113, E7059–E7068. doi: 10.1073/pnas.1615671113

CrossRef Full Text | Google Scholar

Rajgopal, R., Bear, M., Butcher, M. K., and Shaughnessy, S. G. (2008). The effects of heparin and low molecular weight heparins on bone. Thromb. Res. 122, 293–298. doi: 10.1016/j.thromres.2006.10.025

PubMed Abstract | CrossRef Full Text | Google Scholar

Reddigari, S. R., Kuna, P., Miragliotta, G., Shibayama, Y., Nishikawa, K., and Kaplan, A. P. (1993). Human high molecular weight kininogen binds to human umbilical vein endothelial cells via its heavy and light chains. Blood 81, 1306–1311.

PubMed Abstract | Google Scholar

Regoli, D., Calo, G., Rizzi, A., Bogoni, G., Gobeil, F., Campobasso, C., et al. (1996). Bradykinin receptors and receptor ligands (with special emphasis on vascular receptors). Regul. Pept. 65, 83–89. doi: 10.1016/0167-0115(96)00076-6

PubMed Abstract | CrossRef Full Text

Renné, T., and Müller-Esterl, W. (2001). Cell surface-associated chondroitin sulfate proteoglycans bind contact phase factor H-kininogen. FEBS Lett. 500, 36–40. doi: 10.1016/S0014-5793(01)02570-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Renné, T., Dedio, J., David, G., and Müller-Esterl, W. (2000). High molecular weight kininogen utilizes heparan sulfate proteoglycans for accumulation on endothelial cells. J. Biol. Chem. 275, 33688–33696. doi: 10.1074/jbc.M000313200

PubMed Abstract | CrossRef Full Text | Google Scholar

Renné, T., Dedio, J., Meijers, J. C., Chung, D., and Müller-Esterl, W. (1999). Mapping of the discontinuous H-kininogen binding site of plasma prekallikrein. Evidence for a critical role of apple domain-2. J. Biol. Chem. 274, 25777–25784. doi: 10.1074/jbc.274.36.25777

PubMed Abstract | CrossRef Full Text | Google Scholar

Renné, T., Schuh, K., and Müller-Esterl, W. (2005). Local bradykinin formation is controlled by glycosaminoglycans. J. Immunol. 175, 3377–3385. doi: 10.4049/jimmunol.175.5.3377

PubMed Abstract | CrossRef Full Text | Google Scholar

Rocha e Silva, M., Beraldo, W. T., and Rosenfeld, G. (1949). Bradykinin, hypotensive and smooth muscle stimulating factor released from plasma globulin by snake venoms and by trypsin. Am. J. Physiol. 156, 261–273.

PubMed Abstract | Google Scholar

Røjkjaer, R., Hasan, A. A., Motta, G., Schousboe, I., and Schmaier, A. H. (1998). Factor XII does not initiate prekallikrein activation on endothelial cells. Thromb. Haemost. 80, 74–81.

Google Scholar

Sahoo, M., Del Barrio, L., Miller, M. A., and Re, F. (2014). Neutrophil elastase causes tissue damage that decreases host tolerance to lung infection with burkholderia species. PLoS Pathog. 10:e1004327. doi: 10.1371/journal.ppat.1004327

PubMed Abstract | CrossRef Full Text | Google Scholar

Schmaier, A. H. (2016). The contact activation and kallikrein-kinin systems: pathophysiologic and physiologic activities. J. Thromb. Haemost. 14, 28–39. doi: 10.1111/jth.13194

PubMed Abstract | CrossRef Full Text | Google Scholar

Schmaier, A. H., and McCrae, K. R. (2007). The plasma kallikrein-kinin system: its evolution from contact activation. J. Thromb. Haemost. 5, 2323–2329. doi: 10.1111/j.1538-7836.2007.02770.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Stuardo, M., Gonzalez, C. B., Nualart, F., Boric, M., Corthorn, J., Bhoola, K. D., et al. (2004). Stimulated human neutrophils form biologically active kinin peptides from high and low molecular weight kininogens. J. Leukoc. Biol. 75, 631–640. doi: 10.1189/jlb.1103546

PubMed Abstract | CrossRef Full Text | Google Scholar

Veronez, C. L., Nascimento, F. D., Melo, K. R., Nader, H. B., Tersariol, I. L., and Motta, G. (2014). The involvement of proteoglycans in the human plasma prekallikrein interaction with the cell surface. PLoS ONE 9:e91280. doi: 10.1371/journal.pone.0091280

PubMed Abstract | CrossRef Full Text | Google Scholar

Vogel, R., Kaufmann, J., Chung, D. W., Kellermann, J., and Müller-Esterl, W. (1990). Mapping of the prekallikrein-binding site of human H-kininogen by ligand screening of lambda gt11 expression libraries. Mimicking of the predicted binding site by anti-idiotypic antibodies. J. Biol. Chem. 265, 12494–12502.

PubMed Abstract | Google Scholar

Wiernicki, I., Stachowska, E., Safranow, K., Cnotliwy, M., Rybicka, M., Kaczmarczyk, M., et al. (2010). Enhanced matrix-degrading proteolytic activity within the thin thrombus-covered wall of human abdominal aortic aneurysms. Atherosclerosis 212, 161–165. doi: 10.1016/j.atherosclerosis.2010.04.033

PubMed Abstract | CrossRef Full Text | Google Scholar

Yanagishita, M., and Hascall, V. C. (1992). Cell surface heparan sulfate proteoglycans. J. Biol. Chem. 267, 9451–9454.

PubMed Abstract | Google Scholar

Zidane, 2000 Zidane, M., Schram, M. T., Planken, E. W., Molendijk, W. H., Rosendaal, F. R., van der Meer, F. J., et al. (2000). Frequency of major hemorrhage in patients treated with unfractionated intravenous heparin for deep venous thrombosis or pulmonary embolism: a study in routine clinical practice. Arch. Intern. Med. 160, 2369–2373. doi: 10.1001/archinte.160.15.2369

PubMed Abstract | CrossRef Full Text | Google Scholar