Original Research ARTICLE
Ultra performance liquid chromatography and high resolution mass spectrometry for the analysis of plant lipids
- Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany
Holistic analysis of lipids is becoming increasingly popular in the life sciences. Recently, several interesting, mass spectrometry-based studies have been conducted, especially in plant biology. However, while great advancements have been made we are still far from detecting all the lipids species in an organism. In this study we developed an ultra performance liquid chromatography-based method using a high resolution, accurate mass, mass spectrometer for the comprehensive profiling of more than 260 polar and non-polar Arabidopsis thaliana leaf lipids. The method is fully compatible to the commonly used lipid extraction protocols and provides a viable alternative to the commonly used direct infusion-based shotgun lipidomics approaches. The whole process is described in detail and compared to alternative lipidomic approaches. Next to the developed method we also introduce an in-house developed database search software (GoBioSpace), which allows one to perform targeted or un-targeted lipidomic and metabolomic analysis on mass spectrometric data of every kind.
Keywords: lipidomics, ultra performance liquid chromatography, high resolution mass spectrometry, accurate mass, database, all-ion fragmentation, Arabidopsis thaliana, metabolomics
Citation: Hummel J, Segu S, Li Y, Irgang S, Jueppner J and Giavalisco P (2011) Ultra performance liquid chromatography and high resolution mass spectrometry for the analysis of plant lipids. Front. Plant Sci. 2:54. doi: 10.3389/fpls.2011.00054
Received: 08 May 2011;
Accepted: 05 September 2011;
Published online: 12 October 2011.
Edited by:Alisdair Fernie, Max Planck Institute for Plant Physiology, Germany
Reviewed by:Kazuki Saito, Chiba University, Japan
Asaph Aharoni, Weizmann Institute of Science, Israel
Copyright: © 2011 Hummel, Segu, Li, Irgang, Jueppner and Giavalisco. This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.
*Correspondence: Patrick Giavalisco, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Golm, 14476 Potsdam, Germany. e-mail: email@example.com