Impact Factor
This article is part of the Research Topic Regulatory Elements in RNA

Original Research ARTICLE

Front. Plant Sci., 13 December 2011 | http://dx.doi.org/10.3389/fpls.2011.00098

Evidence for a DNA-based mechanism of intron-mediated enhancement

Alan B. Rose*, Shahram Emami, Keith Bradnam and Ian Korf
  • Molecular and Cellular Biology, University of California, Davis, CA, USA

Many introns significantly increase gene expression through a process termed intron-mediated enhancement (IME). Introns exist in the transcribed DNA and the nascent RNA, and could affect expression from either location. To determine which is more relevant to IME, hybrid introns were constructed that contain sequences from stimulating Arabidopsis thaliana introns either in their normal orientation or as the reverse complement. Both ends of each intron are from the non-stimulatory COR15a intron in their normal orientation to allow splicing. The inversions create major alterations to the sequence of the transcribed RNA with relatively minor changes to the DNA structure. Introns containing portions of either the UBQ10 or ATPK1 intron increased expression to a similar degree regardless of orientation. Also, computational predictions of IME improve when both intron strands are considered. These findings are more consistent with models of IME that act at the level of DNA rather than RNA.

Keywords: gene expression, intron-mediated enhancement, Arabidopsis

Citation: Rose AB, Emami S, Bradnam K and Korf I (2011) Evidence for a DNA-based mechanism of intron-mediated enhancement. Front. Plant Sci. 2:98. doi: 10.3389/fpls.2011.00098

Received: 08 September 2011; Accepted: 30 November 2011;
Published online: 13 December 2011.

Edited by:

Anireddy S. N. Reddy, Colorado State University, USA

Reviewed by:

William Brad Barbazuk, University of Florida, USA
Steve M. Mount, University of Maryland, USA

Copyright: © 2011 Rose, Emami, Bradnam and Korf. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

*Correspondence: Alan B. Rose, Molecular and Cellular Biology, University of California, 1 Shields Avenue, Davis, CA 95616, USA. e-mail: abrose@ucdavis.edu