TY - JOUR AU - Jayaraman, Dhileepkumar AU - Forshey, Kari AU - Grimsrud, Paul AU - Ané, Jean-Michel PY - 2012 M3 - Mini Review TI - Leveraging Proteomics to Understand Plant–Microbe Interactions JO - Frontiers in Plant Science UR - https://www.frontiersin.org/articles/10.3389/fpls.2012.00044 VL - 3 SN - 1664-462X N2 - Understanding the interactions of plants with beneficial and pathogenic microbes is a promising avenue to improve crop productivity and agriculture sustainability. Proteomic techniques provide a unique angle to describe these intricate interactions and test hypotheses. The various approaches for proteomic analysis generally include protein/peptide separation and identification, but can also provide quantification and the characterization of post-translational modifications. In this review, we discuss how these techniques have been applied to the study of plant–microbe interactions. We also present some areas where this field of study would benefit from the utilization of newly developed methods that overcome previous limitations. Finally, we reinforce the need for expanding, integrating, and curating protein databases, as well as the benefits of combining protein-level datasets with those from genetic analyses and other high-throughput large-scale approaches for a systems-level view of plant–microbe interactions. ER -