Plant cell walls are highly dynamic and heterogeneous structures, which vary between cell types, growth stages but also between microdomains within a single cell wall. In this review, we summarize the imaging techniques using fluorescent tags that are currently being used and which should in the coming years revolutionize our understanding of the dynamics of cell wall architecture and the cellular processes involved in the synthesis of cell wall components.
Keywords: plant cell-wall, fluorescence microscopy
Citation: Gonneau M, Höfte H and Vernhettes S (2012) Fluorescent tags to explore cell wall structure and dynamics. Front. Plant Sci. 3:145. doi: 10.3389/fpls.2012.00145
Received: 15 March 2012; Accepted: 13 June 2012;
Published online:03 July 2012.
Edited by:Jose Manuel Estevez, University of Buenos Aires, Argentina
Reviewed by:Staffan Persson, Max-Planck Gesellschaft, Germany
Copyright: © 2012 Gonneau, Höfte and Vernhettes. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.
*Correspondence: Samantha Vernhettes, Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, Bâtiment 2, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), 78026 Versailles Cedex, France. e-mail: firstname.lastname@example.org
Abbreviations: BiFC, bimolecular fluorescence complementation; GFP, green fluorescent protein; FESEM, field emission scanning electron microscopy; FLIP, fluorescence loss in photobleaching; FPALM, fluorescence photoactivated localization microscopy; FRAP, fluorescence recovery after photobleaching; FRET, fluorescence resonance energy transfer; LSCM, laser scanning confocal microscopy; LSFM, light-sheet-based fluorescence microscopy; PALM, photoactivated localization microscopy; SDCM, spinning disk confocal microscopy; SIM, structured illumination microscopy; SPIM, selective plane illumination; STED, stimulated emission depletion; STORM, stochastic optical reconstruction microscopy; TEM, transmission electron microscopy; TIRFM, total internal reflection fluorescence microscopy; VAEM, variable-angle epifluorescence microscopy; YFP, yellow fluorescent protein.