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This article is part of the Research Topic The Legume Genome


Original Research ARTICLE

Front. Plant Sci., 29 August 2013 | http://dx.doi.org/10.3389/fpls.2013.00318

A comparison of the molecular organization of genomic regions associated with resistance to common bacterial blight in two Phaseolus vulgaris genotypes

Gregory Perry1*, Claudia DiNatale2, Weilong Xie3, Alireza Navabi3, Yarmilla Reinprecht1, William Crosby2, Kangfu Yu4, Chun Shi4 and K. Peter Pauls1
  • 1Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada
  • 2Department of Biological Sciences, University of Windsor, Windsor, ON, Canada
  • 3Agriculture and Agri-Food Canada, c/o Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada
  • 4Greenhouse and Processing Crops Research Centre, Agriculture and Agri-Food Canada, Harrow, ON, Canada

Resistance to common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli, in Phaseolus vulgaris is conditioned by several loci on different chromosomes. Previous studies with OAC-Rex, a CBB-resistant, white bean variety of Mesoamerican origin, identified two resistance loci associated with the molecular markers Pv-CTT001 and SU91, on chromosome 4 and 8, respectively. Resistance to CBB is assumed to be derived from an interspecific cross with Phaseolus acutifolius in the pedigree of OAC-Rex. Our current whole genome sequencing effort with OAC-Rex provided the opportunity to compare its genome in the regions associated with CBB resistance with the v1.0 release of the P. vulgaris line G19833, which is a large seeded bean of Andean origin, and (assumed to be) CBB susceptible. In addition, the genomic regions containing SAP6, a marker associated with P. vulgaris-derived CBB-resistance on chromosome 10, were compared. These analyses indicated that gene content was highly conserved between G19833 and OAC-Rex across the regions examined (>80%). However, fifty-nine genes unique to OAC Rex were identified, with resistance gene homologues making up the largest category (10 genes identified). Two unique genes in OAC-Rex located within the SU91 resistance QTL have homology to P. acutifolius ESTs and may be potential sources of CBB resistance. As the genomic sequence assembly of OAC-Rex is completed, we expect that further comparisons between it and the G19833 genome will lead to a greater understanding of CBB resistance in bean.

Keywords: common bacterial blight, next generation sequencing, disease resistance, comparative genomics, phaseolus vulgaris

Citation: Perry G, DiNatale C, Xie W, Navabi A, Reinprecht Y, Crosby W, Yu K, Shi C and Pauls KP (2013) A comparison of the molecular organization of genomic regions associated with resistance to common bacterial blight in two Phaseolus vulgaris genotypes. Front. Plant Sci. 4:318. doi: 10.3389/fpls.2013.00318

Received: 25 May 2013; Accepted: 29 July 2013;
Published online: 29 August 2013.

Edited by:

Georgina Hernandez, Centro de Ciencias Genomicas-Universidad Nacional Autonoma de Mexico, Mexico

Reviewed by:

Francesca Sparvoli, Institute of Agricultural Biology and Biotechnology, Italy
Oswaldo Valdes-Lopez, University of Wisconsin-Madison, USA

Copyright © 2013 Perry, DiNatale, Xie, Navabi, Reinprecht, Crosby, Yu, Shi and Pauls. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Gregory Perry, Department of Plant Agriculture, University of Guelph, 50 Stone Road East, Guelph, N1G 2W1 ON, Canada e-mail: perryg@uoguelph.ca