Uncovering plant-pathogen crosstalk through apoplastic proteomic studies
- Laboratoire Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne-EA 4707, Université de Reims Champagne-Ardenne, Reims, France
Plant pathogens have evolved by developing different strategies to infect their host, which in turn have elaborated immune responses to counter the pathogen invasion. The apoplast, including the cell wall and extracellular space outside the plasma membrane, is one of the first compartments where pathogen-host interaction occurs. The plant cell wall is composed of a complex network of polysaccharides polymers and glycoproteins and serves as a natural physical barrier against pathogen invasion. The apoplastic fluid, circulating through the cell wall and intercellular spaces, provides a means for delivering molecules and facilitating intercellular communications. Some plant-pathogen interactions lead to plant cell wall degradation allowing pathogens to penetrate into the cells. In turn, the plant immune system recognizes microbial- or damage-associated molecular patterns (MAMPs or DAMPs) and initiates a set of basal immune responses, including the strengthening of the plant cell wall. The establishment of defense requires the regulation of a wide variety of proteins that are involved at different levels, from receptor perception of the pathogen via signaling mechanisms to the strengthening of the cell wall or degradation of the pathogen itself. A fine regulation of apoplastic proteins is therefore essential for rapid and effective pathogen perception and for maintaining cell wall integrity. This review aims to provide insight into analyses using proteomic approaches of the apoplast to highlight the modulation of the apoplastic protein patterns during pathogen infection and to unravel the key players involved in plant-pathogen interaction.
Keywords: apoplast, cell wall, proteomics, secretome, pathogen, defense, MAMP
Citation: Delaunois B, Jeandet P, Clément C, Baillieul F, Dorey S and Cordelier S (2014) Uncovering plant-pathogen crosstalk through apoplastic proteomic studies. Front. Plant Sci. 5:249. doi: 10.3389/fpls.2014.00249
Received: 14 February 2014; Accepted: 15 May 2014;
Published online: 03 June 2014.
Edited by:Jean-Pierre Metraux, Université de Fribourg, Switzerland
Reviewed by:Murray Grant, University of Exeter, UK
Richard Bostock, University of California, Davis, USA
Copyright © 2014 Delaunois, Jeandet, Clément, Baillieul, Dorey and Cordelier. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Sylvain Cordelier, Laboratoire Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne-EA 4707, Université de Reims Champagne-Ardenne, Moulin de la Housse – BP 1039, 51687 Reims cedex 2, France e-mail: firstname.lastname@example.org