@ARTICLE{10.3389/fpls.2016.00412, AUTHOR={Corbin, Jasmine M. and Hashimoto, Bryce I. and Karuppanan, Kalimuthu and Kyser, Zachary R. and Wu, Liying and Roberts, Brian A. and Noe, Amy R. and Rodriguez, Raymond L. and McDonald, Karen A. and Nandi, Somen}, TITLE={Semicontinuous Bioreactor Production of Recombinant Butyrylcholinesterase in Transgenic Rice Cell Suspension Cultures}, JOURNAL={Frontiers in Plant Science}, VOLUME={7}, YEAR={2016}, URL={https://www.frontiersin.org/articles/10.3389/fpls.2016.00412}, DOI={10.3389/fpls.2016.00412}, ISSN={1664-462X}, ABSTRACT={An active and tetrameric form of recombinant butyrylcholinesterase (BChE), a large and complex human enzyme, was produced via semicontinuous operation in a transgenic rice cell suspension culture. After transformation of rice callus and screening of transformants, the cultures were scaled up from culture flask to a lab scale bioreactor. The bioreactor was operated through two phases each of growth and expression. The cells were able to produce BChE during both expression phases, with a maximum yield of 1.6 mg BChE/L of culture during the second expression phase. Cells successfully regrew during a 5-day growth phase. A combination of activity assays and Western blot analysis indicated production of an active and fully assembled tetramer of BChE.} }