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Front. Plant Sci. | doi: 10.3389/fpls.2017.01300

Adaptation of the pivotal-differential genome pattern for the induction of intergenomic chromosome recombination in hybrids of synthetic amphidiploids within Triticeae tribe

Michał T. Kwiatek1*, Joanna Majka2, Maciej Majka1, Jolanta Belter1 and Halina Wiśniewska1
  • 1Department of Genomics, Institute of Plant Genetics (PAN), Poland
  • 2Department of Environmental Stress Biology, Institute of Plant Genetics (PAN), Poland

A pivotal-differential evolution pattern is when two allopolyploids share a common genome, which is called pivotal, and differ with respect to the other genome or genomes, called differential. This feature induces the intergenomic recombination between chromosomes of differential genomes, which can lead to speciation. Our study is a cytomolecular insight into this mechanism which was adapted for the induction of intergenomic chromosome recombination in hybrids of synthetic amphidiploids Aegilops biuncialis × S. cereale (UUMMRR) and triticale (AABBRR) where R-genome was pivotal. We observed chromosome recombination events which were induced by both: (1) random chromosome fragmentation and non-homologous chromosome end joining at mitosis of root meristem cells and (2) intergenomic chromosome associations at meiosis of pollen mother cells (PMCs) of F1 hybrids. Reciprocal chromosome translocations were identified in six F1 plants and 15 plants of F2 generation using fluorescence in situ hybridization (FISH) with DNA clones (pTa-86, pTa-k374, pTa-465, pTa-535, pTa-k566 and pTa-713). We observed signals of pTa-86, pTa-535 and pTa-k566 probes in several chromosome breakpoints. The comparison of the DNA clone sequences distinguished a number of common motifs, which can be considered as characteristics of chromosome breakpoint loci. Immunodetection of synaptonemal complex proteins and genomic in situ hybridization analysis at meiosis of PMCs of F1 hybrids showed, that the homologous pairing of pivotal R - genome chromosomes is crucial for the fertility of F1 hybrids, however, these chromosomes can be also involved in the intergeneric recombination.

Keywords: Allopolyploids, Chromosome recombination, evolution, Meiosis, Mitosis, pivotal-differential theory, Triticeae

Received: 25 May 2017; Accepted: 10 Jul 2017.

Edited by:

Changbin Chen, University of Minnesota, United States

Reviewed by:

Kai Wang, Fujian Agriculture and Forestry University, China
Isabelle Colas, James Hutton Institute, United Kingdom  

Copyright: © 2017 Kwiatek, Majka, Majka, Belter and Wiśniewska. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Michał T. Kwiatek, Institute of Plant Genetics (PAN), Department of Genomics, Poznan, Poland, mkwi@igr.poznan.pl