Impact Factor 4.298

The 1st most cited journal in Plant Sciences

Original Research ARTICLE

Front. Plant Sci., 25 July 2017 | https://doi.org/10.3389/fpls.2017.01300

Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe

Michal T. Kwiatek1*, Joanna Majka2, Maciej Majka1, Jolanta Belter1 and Halina Wisniewska1
  • 1Cereal Genomics Team, Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland
  • 2Cytogenetics and Molecular Physiology of Plants Team, Department of Environmental Stress Biology, Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland

A pivotal-differential evolution pattern is when two allopolyploids share a common genome, which is called pivotal, and differ with respect to the other genome or genomes, called differential. This feature induces the intergenomic recombination between chromosomes of differential genomes, which can lead to speciation. Our study is a cytomolecular insight into this mechanism which was adapted for the induction of intergenomic chromosome recombination in hybrids of synthetic amphidiploids Aegilops biuncialis × S. cereale (UUMMRR) and triticale (AABBRR) where R-genome was pivotal. We observed chromosome recombination events which were induced by both: (1) random chromosome fragmentation and non-homologous chromosome end joining at mitosis of root meristem cells and (2) intergenomic chromosome associations at meiosis of pollen mother cells (PMCs) of F1 hybrids. Reciprocal chromosome translocations were identified in six F1 plants and 15 plants of F2 generation using fluorescence in situ hybridization (FISH) with DNA clones (pTa-86, pTa-k374, pTa-465, pTa-535, pTa-k566, and pTa-713). We observed signals of pTa-86, pTa-535, and pTa-k566 probes in several chromosome breakpoints. The comparison of the DNA clone sequences distinguished a number of common motifs, which can be considered as characteristics of chromosome breakpoint loci. Immunodetection of synaptonemal complex proteins and genomic in situ hybridization analysis at meiosis of PMCs of F1 hybrids showed, that the homologous pairing of pivotal R—genome chromosomes is crucial for the fertility of F1 hybrids, however, these chromosomes can be also involved in the intergeneric recombination.

Introduction

Intergeneric cross-hybridizations and mutations are key factors in the evolution of higher plants. Polyploidization is a specific kind of mutation, which results in the inheritance of an additional set (or sets) of chromosomes. Polyploidy is common in plants and some reports suggest that 50–70% of living plant species are polyploid (Masterson, 1994; Rieseberg and Willis, 2007) and many lineages show evidence of ancient polyploidy (paleopolyploidy) in their genomes (Otto, 2007). Polyploid plants can appear spontaneously by abnormal cell division (meiotic or mitotic failures) or by fusion of unreduced gametes, which result in chromosome set multiplication (Comai, 2005). The multiplied sets of chromosomes may originate from the same or a closely related individual (“autopolyploid”) or from the cross-hybridization of two different species (“allopolyploidy”). Both autopolyploids (e.g., potato) and allopolyploids (e.g., canola, wheat, triticale) are common among domesticated plant species.

The wheat group is an excellent example of allopolyploidization, through hybridization among species from the plant genera Aegilops and Triticum, also known as an Aegilops-Triticum complex. There are two explanations of speciation in this group. First, monophyletic evolution hypothesis is that a single wild progenitor was an ancestor of particular species or genomes. Second theory, called polyphyletic evolution, says that the wild progenitor could have been introduced into several spontaneous crosses with other species and faced with multiple events of recombination (Zohary, 1999). According to the polyphyletic hypothesis, the rate of parental genome modification in the case of evolution of polyploid species is different. In this instance, one genome is closely related or even identical to the parental one (pivotal genome), while the second—differential genome (or genomes) is much more genetically diversified (Zohary and Feldman, 1962; Feldman and Levy, 2012). For example, these two theories are adapted for explaining the B-genome origin of wheat. Polyploid wheats include two evolutionary lineages: Emmer wheats (AuAuBB) and Timopheevi wheats (AuAuGG) (Spoor, 2001) where Au-genome is the pivotal one. Both of them are supposed to have originated from two independent crosses involving progenitors of Triticum urartu Thum. ex Gandil (Au-genome, paternal component) and Aegilops speltoides Tausch (S-genome, maternal component). On the one side, it is hypothesized that B-genome is monophyletic in origin and was derived directly from Ae. speltoides (Maestra and Naranjo, 1998; Salse et al., 2008). On the other side, B-genome is of polyphyletic origin, and it is assumed that it has derived from more than one diploid species (Jiang and Gill, 1994). Moreover, two pivotal genomes, D and U, were identified in Aegilops genus, where all polyploid species were subdivided into two clusters. The D-genome cluster includes a diploid Ae. tauschii and six polyploid species of Vertebrata and Cylindropyron sections, while the U-genome cluster included a diploid Ae. umbellulata and eight polyploid species of Pleionathera section (Feldman, 1965). D-genome is combined with C-genome (DDCC; Ae. cylindrica), M-genome (DDMM; Ae. crassa), N-genome (DDNN, Ae. ventricosa), M-and S-genome (DDMMSS, Ae. vavilovii) and M-and U-genome (DDMMUU; Ae. juvenalis). Furthermore, in species of Pleionathera section, the U-genome is combined with C-genome (UUCC; Ae. triuncialis), S-genome (UUSS; Ae. variabilis and Ae. kotschyi), M-genomes (UUMM; Ae. geniculata, Ae. biuncialis, Ae. columnaris, Ae. triaristata) or M- and N-genome (UUMMNN, Ae. triaristata) (Kihara, 1954; Kilian et al., 2011).

Previous reports showed that the stabilization of allopolyploids involves: (1) chromosome diploidization, when meiotic pairing of homoeologous chromosomes is suppressed, which results in a diploid-like pairing pattern; and (2) genetic diploidization, in which duplicate genes are either silenced or expressed at reduced levels (Ozkan et al., 2001). In the case of wheat, two dominant genes, Ph1 and Ph2 (minor effect) are recognized to be responsible for the suppressing of homologous pairing (Riley and Chapman, 1958; Sears, 1976; Griffiths et al., 2006). Hence, during meiosis (prophase I), homologous chromosomes are coupled along their length by the loading of a protein structure, called the synaptonemal complex (SC), composed of two proteins ASY-1 and ZYP-1 (Zickler and Kleckner, 1999; Jenkins et al., 2008). Within this framework, homolog chromosomes can recombine. The expression of Ph genes prevents multivalent formation with its obvious consequences for uneven chromosome segregation. In other words, wheat evolved a pairing control system that enforces strictly homologous bivalent pairing.

In contrary, hybrids between related allopolyploids are common and evolved under the pivotal-differential pattern (Zohary and Feldman, 1962; Feldman, 1965; Levy and Feldman, 2002). The key feature of this type of evolution is the presence of two allopolyploids sharing a common genome or genomes and being distinguished at once by other genomes. When these two allopolyploids hybridize, the hybrid has the pivotal genomes presented twice and other genomes that differ from each other (differential genomes) at haploid stage. Such hybrids occur naturally in A. Triticum complex and are fertile (Kimber and Feldman, 1987). It is supposed that initial fertility in such forms is related to a buffering effect of the pivotal genome. Furthermore, rearrangements of the differential genomes subsequently occur following a recombination between them. Backcrossing of such secondary amphiploids to its parents will produce the progeny, which is phenotypically and genotypically different from the parental allotetraploids (Kimber and Yen, 1988).

Generating synthetic amphidiploids containing the genomes of different cereal species provides new insights into polyploid evolution, which can help to understand the mechanism and evolutionary aspects of polyploidy. It can also facilitate the transmission of valuable genetic properties from wild species to cultivated plants (Apolinarska et al., 2010; Kwiatek et al., 2012, 2013). Studies of synthetic allopolyploids obtained within Brassica or Aegilops-Triticale complex have revealed that polyploidization can lead to rapid and extensive genome changes (Song et al., 1995; Feldman et al., 1997; Liu et al., 1998a,b). It was reported that newly synthesized allopolyploids of wheat and Aegilops are characterized by the extensive genome changes at the molecular level, such as rapid elimination of specific low-copy DNA sequences (Ozkan et al., 2001; Shaked et al., 2001).

The main objective of this research was to induce the recombination events between chromosomes of Aegilops biuncialis and triticale subgenomes. For that purpose, we have crossed Aegilops-Secale amphidiploid forms (UUMMRR) with triticale (AABBRR). With this strategy, we have obtained F1 plants containing doubled R-genome chromosomes (pivotal genome, in this case) and complete sets of monosomic A-, B-, U-, and M-genome chromosomes. In the present work, we have karyotyped the F1 plants using fluorescence in situ hybridization (FISH), and examined the pairing interactions of chromosomes during meiosis of pollen mother cells of F1 plants using genomic in situ hybridization (GISH) and immunodetection of proteins involved in synaptonemal complex (SC) formation. Moreover, we have established the sequence patterns of certain breakpoints loci in chromosomes carrying alien chromatin translocations. Finally, the self-pollination efficiency of F1 plants has been calculated.

Materials and Methods

Plant Material

Seeds of two accessions of A. biuncialis Eig. (Ab5—14712 and Ab7—14716, respectively) were kindly provided by Prof. Moshe Feldman (Weizmann Institute of Science, Rehovot, Israel) and used for karyotyping. Amphiploid forms of Ae. biuncialis × S. cereale (Wojciechowska and Pudelska, 2002, 2005) were used for reciprocal hybridizations with hexaploid triticale (2n = 6x = 42; AABBRR; Bogo, Kitaro, Lamberto, Moreno, and Sekundo cultivars; Table 1). Crossing combinations were divided into four following groups: (1) (Ae. biuncialis Ab5 × S. cereale) × triticale (pollinator), (2) (Ae. biuncialis Ab7 × S. cereale) × triticale (pollinator), (3) triticale × (Ae. biuncialis Ab5 × S. cereale; pollinator) and (4) triticale × (Ae. biuncialis Ab7 × S. cereale; pollinator). The fertility was scored by counting the number of grains per number of pollinated flowers (Table 1). Analysis of variance (ANOVA) was conducted to determine the effect of the crossing combination on fertility. The set of F1 plants was germinated on a wet filter paper in the dark at room temperature for 4 days. Thereafter, the seedlings were transferred to soil for 6–8 weeks under short-day conditions (8 h light/16 h dark, 20°C/18°C). Afterwards, the plants were transferred for 6 weeks to vernalizing conditions (10 h light/14 h dark, 4°C) and returned to standard greenhouse conditions (13 h light/11 h dark, 20°C/16°C) to induce flowering. The F2 seeds (Table 2) were collected from the three (previously isolated) spikes after natural desiccation between July and August.

TABLE 1
www.frontiersin.org

Table 1. Efficiency of reciprocal cross-hybridizations between Ae. biuncialis × S. cereale amphiploids and hexaploid triticale.

TABLE 2
www.frontiersin.org

Table 2. Number and origin of F2 seeds after self-pollination of F1 plants.

Chromosome Preparation for FISH/GISH

Accumulation and fixation of mitotic chromosomes were carried out according to Kwiatek et al. (2016a). The digestion was performed in 0.2% (v/v) Onozuka R-10 and Calbiochem cytohelicase (1:1 ratio) and 20% pectinase (Sigma) in 10 mM citrate buffer (pH 4.6) at 37°C for 2 h and 40 min. Metaphase chromosomes were prepared as described by Heckmann et al. (2014) with minor modifications of heating temperature reported by Kwiatek et al. (2017). Meiotic chromosomes were prepared according to Zwierzykowski et al. (2008) with modifications (Kwiatek et al., 2017).

FISH/GISH Experiments

A set of six repetitive sequences: pTa-86, pTa-k374 (28S rDNA), pTa-465, pTa-535, pTa-k566, and pTa-713 were amplified from the clones originated from BAC library of wheat published by Komuro et al. (2013). Sequences were amplified using primers designed by Kwiatek et al. (2016b) and labeled using nick translation kits according to manufacturer instructions (Jena BioScience, Jena, Germany). Probes pTa-86 and pTa-713 were labeled using digoxigenin-11-dUTP (Roche), whereas pTa-535, and pTa-k566 were labeled using tetramethyl-5-dUTP-rhodamine (Roche) and pTa-k374 and pTa-465 were labeled by Atto647 (Jena BioScience). FISH experiments were performed largely as described in Kwiatek et al. (2016b) with the following modifications. Mitotic chromosomes were denatured for 4 min at 75°C, and stringent washes were carried out in 0.1 × SSC at 42°C for 10 min. Meiotic chromosomes were denatured for 3 min at 70°C and a stringent wash was the same as above. Digoxigenin was detected by fluorescein anti-digoxigenin antibody (1:20; Roche). The post-hybridization washes were performed according to Heslop-Harrison (2000) for 30 min, at 42°C in 0,1 × SSC solution. GISH was carried out according to Kwiatek et al. (2012) with modifications (Kwiatek et al., 2016c). DNA of Aegilops comosa Sm. (PI 551020; U.S. National Plant Germplasm System), a progenitor of the M-genome of Ae. biuncialis, was labeled by nick translation with Atto-488 dye (Jena Bioscience), whereas DNA of Ae. umbellulata Zhuk. (U-genome progenitor; PI 222762; U.S. National Plant Germplasm System) was labeled with Atto-550 dye (Jena Bioscience). Total genomic DNA of respective triticales (Bogo, Kitaro, Moreno, Lamberto or Sekundo) was used as an unspecific blocking DNA and was sheared by boiling for 30–45 min and used at a ratio of 1:50 (probe:block). Mitotic and meiotic chromosomes were observed with an Olympus XM10 CCD camera attached to an Olympus BX 61 automatic epifluorescence microscope. Image processing was carried out using Olympus Cell-F (version 3.1; Olympus Soft Imaging Solutions GmbH, Münster, Germany) imaging software with a support of PaintShop Pro X5 software (version 15.0.0.183; Corel Corporation, Ottawa, Canada).

Immunodetection of Synaptonemal Complex Proteins

Meiocytes of F1 plants were embedded in acrylamide in order to keep their three-dimensional architecture as described by Bass et al. (1997), with the modifications (Phillips et al., 2010, 2012). Anthers at the appropriate stage of meiosis were collected into Buffer A (15 mM Pipes-NaOH, pH 6.8, 80 mM KCl, 20 mM NaCl, 0.5 mM EGTA, 2 mM EDTA, 0.15 mM spermine tetra HCL, 0.05 mM spermidine, 1 mM DTT, 0.32 M sorbitol) and fixed for 15 min in freshly prepared 4% paraformaldehyde in Buffer A. Next, anthers were macerated using a brass rod in Buffer A followed by embedding in acrylamide. The pads were incubated for overnight at 4°C in blocking buffer containing anti-ASY1 antibody raised in rabbit (Armstrong et al., 2002) and anti-ZYP1 raised in rat (Higgins et al., 2005) diluted 1:200 and 1:250, respectively. Then, the pads were washed in PBS + 0.1% Tween 20 + 1 mM EDTA pH 8 at room temperature followed by fixation in 2% paraformaldehyde in Buffer A and washed in PBS + 0.1% Tween 20 + 1 mM EDTA pH 8 at room temperature. Finally, pads were mounted in mounting medium (200 mM Tris-HCl pH 8, 2.5% DABCO (1,4-diazobicyclo(2,2,2)octane; Sigma), 80% glycerol and 1 mg/ml DAPI. Nuclei were examined and optically sectioned using a Leica TCS SP5II confocal laser scanning microscope supplied with Leica LAS-AF software.

Comparison of Breakpoint Associated Repetitive Sequences

Sequences of following BAC clones: pTa-86, pTa-535, and pTa-k566 (KC290896, KC290894, and KC290904, respectively; NCBI Genebank), which were mapped in the chromosome breakpoints were compared using Standard Nucleotide Basic Local Alignment Search Tool (Altschul et al., 1990, 1997). The comparison was performed using BLASTn program (NCBI, 2017) to work out the similar sequence motifs.

Results

Production of (Ae. biuncialis × S. cereale) × Triticale F1 and F2 Seeds

In the first step, F1 hybrids were developed by crossing Aegilops-Secale amphidiploid forms (UUMMRR) with triticale (AABBRR) to induce the recombination events between chromosomes of Aegilops and triticale genomes, assuming that R-genome is the pivotal one. The reciprocal hybridizations involved 5170 flowers (pollinated manually), which yielded 271 F1 seeds and the fertility was 5.24% (Table 1).The null hypothesis stated that the means of fertility scores of crossing combinations were equal. Because the p-value was 0.3818, which was greater than the significance level of 0.05, there was not enough evidence to reject the null hypothesis. However, after comparing the overall scores, the fertility was higher, when the A. Secale amphiploids were used as maternal components. F2 seeds (216) were obtained by the self-pollination of spikes (706) of F1 plants, which means that every third self-pollinated spike delivered a F2 seed (Table 2).

Karyotyping of Ae. biuncialis Chromosomes

The FISH technique was applied to mitotic chromosomes of Ae. biuncialis Ab5 (Figure 1A) and Ab7 (Figure 1B), which were used for Ae. biuncialis × S. cereale amphiploids production, followed by reciprocal hybridizations with triticale cultivars. Karyotyping was performed by the signal patterns comparison according to a previous study (Kwiatek et al., 2013) and analogous cytogenetic studies (Schneider et al., 2005; Komuro et al., 2013) in purpose to track Aegilops chromosomes in subsequent generations of (Ae. biuncialis × S. cereale) × triticale hybrids. Moreover, GISH analysis was used to discriminate the U- and M-genome, as well.

FIGURE 1
www.frontiersin.org

Figure 1. Karyograms of Aegilops biuncialis (A) Ab5–14712 and (B) Ab7–14716, showing U- and M-genome chromosomes after FISH with two sets of probes. First set: pTa-86 (green), pTa-k374 (yellow), and pTa-535 (red); second set: pTa-465 (yellow), pTa-k566 (red), and pTa-713 (green). Spearheads indicate centromere locations.

Most of the U-genome and M-genome chromosomes showed similarities of hybridization patterns, comparing Ab5 and Ab7 karyotypes, but some differences were also observed. In both of the Ae. biuncialis accessions analyzed, the pTa-86 probe hybridized mostly to the telomeric region of each chromosome. However, on chromosome 6 UL (Ab 7) and 7 UL (of Ab5 and Ab7), an additional interstitial site was observed. Probe pTa-k374, which indicates 35S rDNA loci, gave strong signals on 1 U chromosomes of both accessions and on 1 M chromosomes of Ab 5. Similarly, pTa-k374 was stronger on chromosome 5 U of Ab7 accession, when compared to Ab5Probe pTa-535 was more abundant on M-genome chromosomes. Comparing both accessions, polymorphisms between pTa-535 signal localization were observed on 6 U, 6 M, and 7 M chromosomes. Signals of pTa-465 were observed mainly in the distal regions of chromosomes. We observed the absence of pTa-465 signals on 6 U and 6 M chromosomes of Ab5 in comparison to Ab7. Signals of pTa-k566 probe were localized in the interstitial regions of 6 U, 1 M, 2 M, 3 M, 4 M, 5 M, and 7 M and in the telomeric regions of 1 U and 4 M. Comparing both accessions, differences in localization of pTa-k566 probe signals were revealed on chromosomes 2 M. Hybridization patterns of pTa-713 probe were the same for 5 U, 6 U, 6 M, and 7 M, however on chromosomes 1 U, 7 U, 2 M, and 5 M interspecific differences were observed. Moreover, this probe did not perform any signals on 2 U, 3 U, and 4 U chromosomes.

Chromosome Identification of (Ae. biuncialis × S. cereale) × Triticale F1 and F2 Hybrids

The mitotic chromosomes of 236 F1 generation plants (Table 3) were distinguished using FISH and genome-assigned using GISH. Ten cells per plant were analyzed, which made 2,360 examined cells, in total. As expected, the chromosome constitution of F1 plants consisted of 14 R-genome chromosomes and 7 A-, 7 B-, 7 U-, and 7 M-genome chromosomes. The total number of chromosomes was 42. However, six plants were detected to carry chromosome translocations (Table 4). Moreover, particular chromosomes involved in those translocation events varied within a plant, to be more precisely—root meristem, which resulted in differences of chromosome constitution between cells of the same meristem were observed. The chromosome translocations encompassed both intergeneric (Aegilops-triticale) and intrageneric (Aegilops-Aegilops) recombination (Figures 2, 3, respectively). In particular, following chromosomes of triticale: 1 B, 4 R, 5 R, 7 R, and Aegilops: 4 U, 5 U, 7 U, 5 M, 6 M, and 7 M were involved in recombination events (Table 4). What is more, the chromosome breakpoint regions overlapped with three FISH probe loci (pTa-86, pTa-535, and pTa-k566; Figures 2d,f). The FISH/GISH analyses of F2 hybrids were performed on 216 plants (2,160 cells, as a consequence). Translocation chromosomes were identified in 15 plants (Table 4). The chromosome number of F2 plants ranged from 35 to 41. As in F1 plants, the same chromosomes were involved in translocation events, with the exception of 7RS.3AS-3AL translocation chromosome, which was identified only in two plants of F2 generation. In the rest of the cases, chromosome 5 M of Ae. biuncialis was involved in intergeneric chromosome recombination with 5 U, 5 R, and 1 B chromosomes. In contrast to the chromosome aberrations observed in F1 plants, all cells of particular root meristem carried the same chromosome set and structure.

TABLE 3
www.frontiersin.org

Table 3. Number and origin of F1 plants karyotyped in the study.

TABLE 4
www.frontiersin.org

Table 4. Chromosome translocations identified in root meristems of F1 and F2 (Ae. biuncialis × S. cereale) × triticale hybrids.

FIGURE 2
www.frontiersin.org

Figure 2. Mitotic chromosomes of (Ae. biuncialis Ab7 × S. cereale) × Sekundo F1 hybrid after (a,c,e) GISH with genomic DNA probes of: Ae. umbellulata (UU; red), Aegilops comosa (MM; green) blocked with genomic DNA of triticale “Sekundo” (A- and B-genome chromosomes—gray, R-genome chromosomes—blue); (b,d,f) FISH with pTa-86 (green), pTa-k374 (yellow), and pTa-535 (red) probes. (c,d) 5MS-5ML.1BL chromosome translocation. (e,f) 1BS-1BL.5ML chromosome translocation. Spearheads indicate the centromere location. Arrows show the chromosome breakpoint localization and associated probe signal.

FIGURE 3
www.frontiersin.org

Figure 3. Mitotic chromosomes of Kitaro × (Ae. biuncialis Ab5 × S. cereale) F1 hybrid after (a,c,e) GISH with genomic DNA probes of: Ae. umbellulata (UU; red), Aegilops comosa (MM; green) blocked with genomic DNA of triticale “Sekundo” (A- and B-genome chromosomes—gray, R-genome chromosomes—blue); (b,d,f) FISH with pTa-86 (green), pTa-k374 (yellow), and pTa-535 (red) probes. (c,d) 5US.6MS-6ML chromosome translocation. (e,f) 6MS.5US-5UL chromosome translocation. Arrows show the chromosome breakpoint localization. Spearheads indicate the centromere location.

Comparison of Fish Probe Sequences Mapped in Chromosome Breakpoint Loci

DNA repetitive sequences of four BAC clones: pTa-86 (KC290896), pTa-535 (KC290894), and pTa-k566 (KC290904) were compared using the BLASTn software. The aim of this approach was to find and dissect DNA motifs which are similar between each BAC sequence. All comparisons resulted in a number of identical or akin motifs (Table 5). The sequences of pTa-86 and pTa-535 clones were most related and shared 20 common DNA motifs, 8–14 nucleotides long. Nevertheless, only five motifs were concerted for pTa-86 and pTa-k566 clone sequences. Neither of those DNA motifs was presented in all BAC clone sequences. The most common motifs consisted of eight bases and were similar to TCAATTTC or TCGGTCAT or TTGACCAAT sequences, considering alternations of certain bases and their order changes.

TABLE 5
www.frontiersin.org

Table 5. Common motifs shared by DNA repetitive sequences of BAC clones mapped in chromosome breakpoints in FISH experiment.

Immunolocalization of Synaptonemal Complex Proteins at Prophase I of Meiosis of Pollen Mother Cells (PMCs) of F1 Hybrids

Chromosome pairing analyses were performed for 28 F1 hybrids, in particular, for 15 plants carrying Ae. biuncialis (Ab5) chromosomes and for 13 plants with Ae. biuncialis (Ab7) chromosomes. Ten PMCs per plant were examined (280, in total). In purpose to track the temporal and spatial expression of the synaptonemal complex, meiocytes were embedded in polyacrylamide gel and two structural proteins of SC (ASY1 and ZYP1) were detected using immunolocalization (Figure 4). At the leptotene, ASY1 proteins formed highly muddled, long and thin linear signals. While the meiotic process proceeds to early zygotene, the ZYP1 linear signals appeared but not co-localized with ASY1 signals. Moreover, it was observed that ASY1 proteins created knot-like, highly condensed zones. At zygotene, the linear signals of ASY1 and ZYP1 thickened similarly with the chromosome condensation. At late zygotene ASY1 loops were visible. When the chromosomes became short and thick like at the pachytene stage, the ZYP1 signals were massive, while the ASY1 signals became wavy. As the diplotene stage began, the signals of both proteins (ASY1 and ZYP1) were thick and curly and in some zones colocalized together. When the diplotene proceeded, the ZYP1 signal disappeared and the ASY1 signal became highly condensed.

FIGURE 4
www.frontiersin.org

Figure 4. Immunodetection of ASY-1 (green) and ZYP-1 (red) foci associated with chromatin (blue) of (Ae. biuncialis Ab7 × S. cereale) × Sekundo F1 hybrid during subsequent stages of prophase I of meiosis of pollen mother cells. Spearheads show highly condensed zones of ASY-1 protein foci.

Evaluation of Chromosome Pairing at Metaphase I of Meiosis of Pollen Mother Cells (PMCs) of F1 Hybrids

Furthermore, pairing between triticale and Ae. biuncialis chromosomes was detected by means of genomic in situ hybridization in metaphase I of meiosis in the F1 hybrid plants. Seven univalents of each of U, M, A, and B genomes were observed in most of PMCs. Moreover, in the majority of cells, the R-genome chromosomes were paired and formed 7 bivalents. However, the intergenomic chromosome pairing was uncovered as well (Table 6, Figure 5). The GISH experiments showed that the homeologous recombination between chromosomes of different genomes was present in the form of bivalents (Figures 5a–f) and trivalents (Figures 5g,h). Moreover, both intraspecific and interspecific recombination events were detected. The ANOVA calculations followed by Tukey's honest significant difference test showed that in some cases the differences between the number of intergeneric chromosome recombination events was significant, which corresponded with the accession of Ae. biuncialis or/and cross-combination type. For example, the intragenomic pairing frequency between U- and M-genomes (Figure 5a) was significantly higher (α = 0.01), while comparing (Ae. biuncialis Ab5 × S. cereale) × triticale and (Ae. biuncialis Ab7 × S. cereale) × triticale hybrids. Bivalents and trivalents consisted of Ae. biuncialis and triticale chromosomes were observed as well. Considering the recombination events between M- and R-genome chromosomes (Figure 5E), it was showed that there were significant differences between bivalent frequencies which were correlated with the type of cross-combination. In this particular case, the M-/R-genome bivalents were observed only in triticale × (Ae. biuncialis × S. cereale) F1 hybrids (amphiploid form as a maternal component). The same correlation was observed for M-/T(A- or B-genome) bivalents formation (Figure 5d) which significantly differed (α = 0.01), considering the origin of the maternal component of the crossing combination. What was interesting, the highest significant (α = 0.05) differences between the means of bivalents, composed of U-genome paired with triticale chromosomes (A-, B- or R-genome), were related to both types of crossing combination and the origin of an amphiploid form (Figures 5b,c). The pairing events within triticale chromosomes, in particular between R-genome and A- or B-genome chromosomes, were also observed (Figure 5f), but did not differ in terms of cross-hybridization types. Meiotic chromosomes of Ae. biuncialis genomes were also associated in trivalents and appeared in all of the crossing combinations. The tendency for U-/M-/U-genome trivalents (Figure 5h) formation was significantly higher (α = 0.01) in triticale × (Ae. biuncialis Ab7 × S. cereale) combination. Remarkably, only one type of trivalent, the one containing chromosomes of both triticale and Ae. biuncialis (U, M, A or B genomes) was identified in (Ae. biuncialis Ab7 × S. cereale) × triticale combination (Figure 5g).

TABLE 6
www.frontiersin.org

Table 6. Intergenomic chromosome associations during meiosis of pollen mother cells in F1 hybrids.

FIGURE 5
www.frontiersin.org

Figure 5. Meiotic chromosomes of (Ae. biuncialis Ab7 × S. cereale) × Sekundo F1 hybrid after GISH with genomic DNA probes of: Ae. umbellulata (UU; red) and Ae. comosa (MM; green) blocked with genomic DNA of triticale “Sekundo” (A- and B-genome chromosomes—gray, R-genome chromosomes—blue). Examples of seven types of intergenomic MI associations: (a) U-/M-genome rod bivalent; (b) T(A- or B-genome)/U-genome rod bivalent; (c) R-/U-genome ring bivalent; (d) T(A- or B-genome)/M-genome rod bivalent; (e) R-/M-genome rod bivalent; (f) T(A- or B-genome)/R-genome rod bivalent; (g) M-/T(A- or B-genome)/U-genome rod trivalent; (h) U-/M-/U-genome V-shape trivalent. Arrows indicate intergenomic chromosome pairing events.

Discussion

In general, F1 hybrids obtained by the intergeneric cross-hybridization are sterile, mostly because of the lack of functional gametes. This is connected with the differences in chromosome origin and number. If parents are of distant genome affinity and differing chromosome pair number, the F1 offspring will be unable to produce chromosomally identical and balanced gametes. However, especially in the evolution of the majority of polyploid plants, those obstacles were overcame with the pivotal-differential origin pattern. In this study, a pivotal-differential evolution mechanism was adapted for the induction of recombination events between chromosomes of different genomes of artificial allopolyploids from A. Triticum complex. Hence, we have used amphiploid forms which carry whole Ae. biuncialis (UUMM) and S. cereale (RR) chromosome sets for reciprocal cross-hybridizations with triticale (AABBRR), which is an artificial cereal, composed of A- and B-genomes of wheat and R-genome of rye (S. cereale L). The genome composition of F1 offspring which carried R-genome chromosomes present twice (pivotal or axial genome) and A-, B-, U-, and M-genome chromosomes occurring in haploid stage, allowed to produce semi-fertile gametes.

The main assumption of this work was to explore the process of the intergenomic chromosome translocations being formed during the pivotal-differential evolutionary pattern. The reciprocal cross-hybridization efficiency between A. Secale amphiploids and triticale was determined by the parental components, however the differences between particular crossing combinations were not significant. Nevertheless, the fertility of F1 plants, which was measured by the seed number per spike characteristic, was similar for all F1 crossing combinations. Hence, it can be said that the presence of pivotal, R-genome chromosomes secured the semi-fertility of F1 plants which were able to produce offspring. This statement was proven by the results of evaluation of the synaptonemal complex formation and chromosome pairing at prophase I and metaphase I of meiosis, respectively. In order to track the progress of synapsis, two proteins of synaptonemal complex, ASY1 and ZYP1, were detected and tracked using immunolocalization in meiocytes at subsequent stages of prophase I of meiosis of PMCs. The SC-associated protein ASY1 marked unpaired axial elements and the transverse filament protein ZYP1 labeled synapsed regions. In diploids, the ASY1 signal disappears in synapsed regions at zygotene and appears again at pachytene, which is supposed to be related to a temporary masking or modification of its epitope or alternatively, the protein is removed from the chromosome axes during zygotene and reloaded during pachytene (Phillips et al., 2012). In our study, the presence of both proteins at zygotene was detected in all PMCs of F1 hybrids, which showed that only a part of pre-condensed chromosomes were able to form synapsis and be paired, as a consequence. Furthermore, ASY1 protein appeared more condensed in several foci at zygotene and early pachytene, and created a knot-like structures. What is interesting, associated regions of chromatin in nuclei have a lower density of DAPI staining, which is characteristic for euchromatin. The same observations were reported by Phillips et al. (2012), who tracked the behavior of telomeres using FISH associated with the immunolocalisation of ASY1 protein and speculated that the co-localization of euchromatin and ASY1 at this stage may be functionally related, and might be connected with the synapsis of telomeres in these areas. In our study, the ASY-1 knot-like foci might indicate the pivotal, R-genome telomere synapsis. However, it is also possible that ASY1 loops are, in fact, in the same region.

The formation of seven R-genome bivalents was observed at metaphase I of PMCs using GISH. However, other chromosome associations were detected, as well. In theory, the presence of two homologs of pivotal, R-genome chromosomes should result in R-genome bivalent formation and lower tendency to create intergenomic chromosome pairing. These conditions should determine the A-, B-, U-, and M-genome univalent presence or intergenomic chromosome associations within those four, differential genomes. However, in our work we observed that in some cases the mean number of U/R or M/R bivalents was comparable or even higher than the mean number of U/T or M/T bivalents. It can be suspected, that Ph1 gene (5 B chromosome) expression, which ensures the recombination only between pairs of homologous chromosomes or/and hampers pairing events between chromosomes from the related (homoeologous) sub-genomes (Griffiths et al., 2006) suppressed the formation of U/T and M/T chromosome associations. For example, Lukaszewski and Kopecky (2010) studied autotetraploid rye with additional chromosomes of wheat and reported that Ph1 gene operates in rye in the same way, like in polyploid wheats and have suggested that it controls a basic mechanism of chromosome recognition (Lukaszewski and Kopecky, 2010). This is the most probable explanation of relatively low appearance of intergenomic chromosome recombination during meiosis of F1 hybrids. Moreover, the means of bivalent and trivalent number of particular chromosome associations differed significantly with respect to the parental components. Based on this fact, it can be said that the tendency to form intergenomic chromosome recombination events could depend on the origin of Ae. biuncialis chromosomes. To be more precise, FISH analysis was performed for the chromosome identification. At the beginning, we karyotyped two Ae. biuncialis accessions (Ab5 and Ab7) which were used for A. Secale amphiploid production (Wojciechowska and Pudelska, 2002, 2005). As expected, M-genome chromosomes showed more differences between the accessions in comparison to U-genome chromosomes, which is in parallel with the pivotal-differental composition of this species (Zohary and Feldman, 1962). Moreover, chromosome 4 M was most polymorphic, which was also reported by Schneider et al. (2005).

Six repetitive DNA sequences used as FISH probes allowed to recognize all chromosomes and segments of translocation chromosomes. Three of them (pTa-86, pTa-535, and pTa-k566) were mapped in chromosome breakpoints, that is in line with the previous reports, showing that chromosome breakpoints appear in regions which are rich in repetitive DNA sequences. The sequences comparison led us to the assumption, that 8-bases motifs, such as TCAATTTC, TCGGTCAT, TTGACCAAT or familiar are characteristic for heterochromatin regions, where the chromosome break formation takes place. Repetitive DNA sequences are able to mobilize through the genome and to change their copy number. Hence, it is hypothesized that these repetitive DNA sequences may promote chromosomal rearrangements. It was reported that satellite DNA families are involved in recombination events in Drosophila (Kuhn et al., 2009) and play an important role in evolution of chromosomes in mammalians (Adega et al., 2009) and plants (Raskina et al., 2008). It was also reported that mini- and microsatellite sequences can be associated with recombination hot spots in humans (Majewski and Ott, 2000) and with chromosomal fragile sites in eukaryotes (Ruiz-Herrera et al., 2006). In plants, an association between chromosome regions carrying simple sequence repeats and intergenomic translocation breakpoints in natural populations of allopolyploid wild wheats was reported, as well (Molnar et al., 2011).

Given the structure of the chromosome breakpoints, we studied the mechanism of the chromosome segments translocation, as well. In general, chromosome aberrations are generated as a result of meiotic abnormalities, which could be induced, amongst others, by the rapid changes in the occurrence of the repetitive sequences (Ozkan et al., 2001). In our study, we observed several chromosome translocations in F2 plants caused by intergenomic chromosome associations during meiosis of PMCs of F1 plants. However, the karyotype analysis of F1 plants revealed that six plants carried cryptic intergenomic chromosome translocations involving chromosome segments from sub-genomes of both parental forms. It could be hypothesized, that rapid genome changes, triggered by the forcible cross-hybridization, resulted in random chromosome fragmentation and non-homologous end joining. DNA double-strand breaks (DSBs) may result from endogenous and exogenous DNA damage agents or mutagenesis (Friedberg et al., 2004). It was reported that cellular defects caused by the irregularities in mitosis can promote chromosome mis-segregation and aneuploidy. Mitotic failures can result in the DNA damage and chromosome breaks, as well (Ganem and Pellman, 2012). Moreover, abnormal mitosis associated with the DNA damage is reported to have potential impact on tumorigenesis in mammalians. We hypothesized, the similar process of random chromosome fragmentation and non-homologous chromosome end joining occurred at mitosis of cells in the root meristems of F1 plants and resulted in a formation of reciprocal translocations (i.e., 1BS–1BL.5 ML and 5MS–5ML.1BL). Moreover, this hypothesis could be endorsed by the heterogeneity of particular chromosome translocations found in different cells of single root meristem. It can be suspected that chromosome fragmentation in this case could be caused by cellular stress (Stevens et al., 2011), however, at the moment we do not have evidence to confirm those statements.

In summary, this study shed some light on the pivotal-differential evolutionary process of speciation. Our results showed that the induction of the intergenomic chromosome translocations in pivotal-differential allopolyploids is a complicated and complex process. There are many genetic and epigenetic factors that could have an influence on this mechanism. We characterized the chromosome translocations and breakpoints and suspected that their localization can be related to the chromosome loci of some similar motifs of repetitive DNA sequences. Our observations revealed that the pivotal genome is crucial for the fertility of F1 hybrids, however, chromosomes of the pivotal genome can be also involved in the intergeneric recombination.

Author Contributions

Conceived and designed the project and experiments: MK. Generated the plant material: MK, JB, and HW. Performed the FISH/GISH experiments: MK, JM, MM. Performed the immunodetection experiments: MK. Performed the clone DNA sequences comparison: MM. Analyzed the data: MK. Wrote the paper: MK.

Funding

This work was supported by the National Science Centre, Kraków, Poland (grant NCN SONATA 6; 2013/11/D/NZ9/02719).

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The authors are grateful to Glyn Jenkins and Dylan Wyn Phillips from the Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Wales, United Kingdom for the supply of the ASY1 and ZYP1 antibodies and for training in confocal microscopy.

References

Adega, F., Guedes-Pinto, H., and Chaves, R. (2009). Satellite DNA in the karyotype evolution of domestic animals-clinical considerations. Cytogenet. Genome Res. 126, 12–20. doi: 10.1159/000245903

PubMed Abstract | CrossRef Full Text | Google Scholar

Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990). Basic local alignment search tool. J. Mol. Biol. 215, 403–410. doi: 10.1016/S0022-2836(05)80360-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402. doi: 10.1093/nar/25.17.3389

PubMed Abstract | CrossRef Full Text | Google Scholar

Apolinarska, B., Wisniewska, H., and Wojciechowska, B. (2010). Aegilops-rye amphiploids and substitution rye used for introgression of genetic material into rye (Secale cereale L.). J. Appl. Genet. 51, 413–420. doi: 10.1007/BF03208871

PubMed Abstract | CrossRef Full Text | Google Scholar

Armstrong, S. J., Caryl, A. P., Jones, G. H., and Franklin, F. C. (2002). Asy1, a protein required for meiotic chromosome synapsis, localizes to axis-associated chromatin in Arabidopsis and Brassica. J. Cell Sci. 115, 3645–3655. doi: 10.1242/jcs.00048

PubMed Abstract | CrossRef Full Text | Google Scholar

Bass, H. W., Marshall, W. F., Sedat, J. W., Agard, D. A., and Cande, W. Z. (1997). Telomeres cluster de novo before the initiation of synapsis: a three-dimensional spatial analysis of telomere positions before and during meiotic prophase. J. Cell Biol. 137, 5–18. doi: 10.1083/jcb.137.1.5

PubMed Abstract | CrossRef Full Text | Google Scholar

Comai, L. (2005). The advantages and disadvantages of being polyploid. Nat. Rev. Genet. 6, 836–846. doi: 10.1038/nrg1711

PubMed Abstract | CrossRef Full Text | Google Scholar

Feldman, M. (1965). Chromosome pairing between differential genomes in hybrids of tetraploid aegilops species. Evolution 19, 563–568. doi: 10.1111/j.1558-5646.1965.tb03333.x

CrossRef Full Text | Google Scholar

Feldman, M., and Levy, A. A. (2012). Genome evolution due to allopolyploidization in wheat. Genetics 192, 763–774. doi: 10.1534/genetics.112.146316

PubMed Abstract | CrossRef Full Text | Google Scholar

Feldman, M., Liu, B., Segal, G., Abbo, S., Levy, A. A., and Vega, J. M. (1997). Rapid elimination of low-copy DNA sequences in polyploid wheat: a possible mechanism for differentiation of homoeologous chromosomes. Genetics 147, 1381–1387.

PubMed Abstract | Google Scholar

Friedberg, E. C., McDaniel, L. D., and Schultz, R. A. (2004). The role of endogenous and exogenous DNA damage and mutagenesis. Curr. Opin. Genet. Dev. 14, 5–10. doi: 10.1016/j.gde.2003.11.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Ganem, N. J., and Pellman, D. (2012). Linking abnormal mitosis to the acquisition of DNA damage. J. Cell Biol. 199, 871–881. doi: 10.1083/jcb.201210040

PubMed Abstract | CrossRef Full Text | Google Scholar

Griffiths, S., Sharp, R., Foote, T. N., Bertin, I., Wanous, M., Reader, S., et al. (2006). Molecular characterization of Ph1 as a major chromosome pairing locus in polyploid wheat. Nature 439, 749–752. doi: 10.1038/nature04434

PubMed Abstract | CrossRef Full Text | Google Scholar

Heckmann, S., Jankowska, M., Schubert, V., Kumke, K., Ma, W., and Houben, A. (2014). Alternative meiotic chromatid segregation in the holocentric plant Luzula elegans. Nat. Commun. 5:4979. doi: 10.1038/ncomms5979

PubMed Abstract | CrossRef Full Text | Google Scholar

Heslop-Harrison, J. S. (2000). Comparative genome organization in plants: from sequence and markers to chromatin and chromosomes. Plant Cell 12, 617–636. doi: 10.1105/tpc.12.5.617

PubMed Abstract | CrossRef Full Text | Google Scholar

Higgins, J. D., Sanchez-Moran, E., Armstrong, S. J., Jones, G. H., and Franklin, F. C. (2005). The Arabidopsis synaptonemal complex protein ZYP1 is required for chromosome synapsis and normal fidelity of crossing over. Genes Dev. 19, 2488–2500. doi: 10.1101/gad.354705

PubMed Abstract | CrossRef Full Text | Google Scholar

Jenkins, G., Phillips, D., Mikhailova, E. I., Timofejeva, L., and Jones, R. N. (2008). Meiotic genes and proteins in cereals. Cytogenet. Genome Res. 120, 291–301. doi: 10.1159/000121078

PubMed Abstract | CrossRef Full Text | Google Scholar

Jiang, J., and Gill, B. S. (1994). Different species-specific chromosome translocations in Triticum timopheevii and T. turgidum support the diphyletic origin of polyploid wheats. Chromosome Res. 2, 59–64. doi: 10.1007/BF01539455

PubMed Abstract | CrossRef Full Text | Google Scholar

Kihara, H. (1954). Considerations on the evolution and distribution of aegilops species based on the analyser-method. Cytologia 19, 336–357. doi: 10.1508/cytologia.19.336

CrossRef Full Text | Google Scholar

Kilian, B., Mammen, K., Millet, E., Sharma, R., Graner, A., Salamini, F., et al. (2011). “Aegilops,” in Wild Crop Relatives: Genomic and Breeding Resources: Cereals, ed C. Kole (Berlin, Heidelberg: Springer Berlin Heidelberg), 1–76.

Kimber, G., and Feldman, M. (1987). Wild Wheat: An introduction. Columbia, MO: College of Agriculture, University of Missouri-Columbia.

Google Scholar

Kimber, G., and Yen, Y. (1988). Analysis of pivotal-differential evolutionary patterns. Proc. Natl. Acad. Sci. U.S.A. 85, 9106–9108. doi: 10.1073/pnas.85.23.9106

PubMed Abstract | CrossRef Full Text | Google Scholar

Komuro, S., Endo, R., Shikata, K., and Kato, A. (2013). Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure. Genome 56, 131–137. doi: 10.1139/gen-2013-0003

PubMed Abstract | CrossRef Full Text | Google Scholar

Kuhn, G. C., Teo, C. H., Schwarzacher, T., and Heslop-Harrison, J. S. (2009). Evolutionary dynamics and sites of illegitimate recombination revealed in the interspersion and sequence junctions of two nonhomologous satellite DNAs in cactophilic Drosophila species. Heredity 102, 453–464. doi: 10.1038/hdy.2009.9

PubMed Abstract | CrossRef Full Text | Google Scholar

Kwiatek, M., Belter, J., Majka, M., and Wisniewska, H. (2016a). Allocation of the S-genome chromosomes of Aegilops variabilis Eig. carrying powdery mildew resistance in triticale (× Triticosecale wittmack). Protoplasma 253, 329–343. doi: 10.1007/s00709-015-0813-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Kwiatek, M., Blaszczyk, L., Wisniewska, H., and Apolinarska, B. (2012). Aegilops-secale amphiploids: chromosome categorisation, pollen viability and identification of fungal disease resistance genes. J. Appl. Genet. 53, 37–40. doi: 10.1007/s13353-011-0071-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Kwiatek, M., Majka, M., Majka, J., Belter, J., Suchowilska, E., Wachowska, U., et al. (2016b). Intraspecific polymorphisms of cytogenetic markers mapped on chromosomes of Triticum polonicum L. PLoS ONE 11:e0158883. doi: 10.1371/journal.pone.0158883

PubMed Abstract | CrossRef Full Text | Google Scholar

Kwiatek, M., Majka, M., Slusarkiewicz-Jarzina, A., Ponitka, A., Pudelska, H., Belter, J., et al. (2016c). Transmission of the Aegilops ovata chromosomes carrying gametocidal factors in hexaploid triticale (× Triticosecale wittm.) hybrids. J. Appl. Genet. 57, 305–315. doi: 10.1007/s13353-015-0332-3

CrossRef Full Text | Google Scholar

Kwiatek, M. T., Wisniewska, H., Slusarkiewicz-Jarzina, A., Majka, J., Majka, M., Belter, J., et al. (2017). Gametocidal factor transferred from Aegilops geniculata Roth can be adapted for large-scale chromosome manipulations in cereals. Front. Plant Sci. 8:409. doi: 10.3389/fpls.2017.00409

PubMed Abstract | CrossRef Full Text | Google Scholar

Kwiatek, M., Wisniewska, H., and Apolinarska, B. (2013). Cytogenetic analysis of Aegilops chromosomes, potentially usable in triticale (× Triticosecale Witt.) breeding. J. Appl. Genet. 54, 147–155. doi: 10.1007/s13353-013-0133-5

PubMed Abstract | CrossRef Full Text | Google Scholar

Levy, A. A., and Feldman, M. (2002). The impact of polyploidy on grass genome evolution. Plant Physiol. 130, 1587–1593. doi: 10.1104/pp.015727

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, B., Vega, J. M., and Feldman, M. (1998a). Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops. II. Changes in low-copy coding DNA sequences. Genome 41, 535–542. doi: 10.1139/g98-052

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, B., Vega, J. M., Segal, G., Abbo, S., Rodova, M., and Feldman, M. (1998b). Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops. I. Changes in low-copy noncoding DNA sequences. Genome 41, 272–277. doi: 10.1139/g98-011

CrossRef Full Text | Google Scholar

Lukaszewski, A. J., and Kopecky, D. (2010). The Ph1 locus from wheat controls meiotic chromosome pairing in autotetraploid rye (Secale cereale L.). Cytogenet. Genome Res. 129, 117–123. doi: 10.1159/000314279

PubMed Abstract | CrossRef Full Text | Google Scholar

Maestra, B., and Naranjo, T. (1998). Homoeologous relationships of Aegilops speltoides chromosomes to bread wheat. Theor. Appl. Genet. 97, 181–186. doi: 10.1007/s001220050883

CrossRef Full Text | Google Scholar

Majewski, J., and Ott, J. (2000). GT repeats are associated with recombination on human chromosome 22. Genome Res. 10, 1108–1114. doi: 10.1101/gr.10.8.1108

PubMed Abstract | CrossRef Full Text | Google Scholar

Masterson, J. (1994). Stomatal size in fossil plants: evidence for polyploidy in majority of angiosperms. Science 264, 421–424. doi: 10.1126/science.264.5157.421

PubMed Abstract | CrossRef Full Text | Google Scholar

Molnar, I., Cifuentes, M., Schneider, A., Benavente, E., and Molnar-Lang, M. (2011). Association between simple sequence repeat-rich chromosome regions and intergenomic translocation breakpoints in natural populations of allopolyploid wild wheats. Ann. Bot. 107, 65–76. doi: 10.1093/aob/mcq215

PubMed Abstract | CrossRef Full Text | Google Scholar

NCBI (2017). Database resources of the national center for biotechnology information. Nucleic Acids Res. 45, D12–D17. doi: 10.1093/nar/gks1189

CrossRef Full Text

Otto, S. P. (2007). The evolutionary consequences of polyploidy. Cell 131, 452–462. doi: 10.1016/j.cell.2007.10.022

PubMed Abstract | CrossRef Full Text | Google Scholar

Ozkan, H., Levy, A. A., and Feldman, M. (2001). Allopolyploidy-induced rapid genome evolution in the wheat (Aegilops-triticum) group. Plant Cell 13, 1735–1747. doi: 10.1105/tpc.13.8.1735

PubMed Abstract | CrossRef Full Text | Google Scholar

Phillips, D., Nibau, C., Ramsay, L., Waugh, R., and Jenkins, G. (2010). Development of a molecular cytogenetic recombination assay for barley. Cytogenet. Genome Res. 129, 154–161. doi: 10.1159/000314335

PubMed Abstract | CrossRef Full Text | Google Scholar

Phillips, D., Nibau, C., Wnetrzak, J., and Jenkins, G. (2012). High resolution analysis of meiotic chromosome structure and behaviour in barley (Hordeum vulgare L.). PLoS ONE 7:e39539. doi: 10.1371/journal.pone.0039539

PubMed Abstract | CrossRef Full Text | Google Scholar

Raskina, O., Barber, J. C., Nevo, E., and Belyayev, A. (2008). Repetitive, D. N. A., and chromosomal rearrangements: speciation-related events in plant genomes. Cytogenet. Genome Res. 120, 351–357. doi: 10.1159/000121084

CrossRef Full Text | Google Scholar

Rieseberg, L. H., and Willis, J. H. (2007). Plant speciation. Science 317, 910–914. doi: 10.1126/science.1137729

PubMed Abstract | CrossRef Full Text | Google Scholar

Riley, R., and Chapman, V. (1958). Genetic control of the cytologically diploid behaviour of hexaploid wheat. Nature 182, 713–715. doi: 10.1038/182713a0

CrossRef Full Text | Google Scholar

Ruiz-Herrera, A., Castresana, J., and Robinson, T. J. (2006). Is mammalian chromosomal evolution driven by regions of genome fragility? Genome Biol. 7:R115. doi: 10.1186/gb-2006-7-12-r115

PubMed Abstract | CrossRef Full Text | Google Scholar

Salse, J., Chagué, V., Bolot, S., Magdelenat, G., Huneau, C., Pont, C., et al. (2008). New insights into the origin of the B genome of hexaploid wheat: evolutionary relationships at the SPA genomic region with the S genome of the diploid relative Aegilops speltoides. BMC Genomics 9:555. doi: 10.1186/1471-2164-9-555

PubMed Abstract | CrossRef Full Text | Google Scholar

Schneider, A., Linc, G., Molnar, I., and Molnar-Lang, M. (2005). Molecular cytogenetic characterization of Aegilops biuncialis and its use for the identification of 5 derived wheat-Aegilops biuncialis disomic addition lines. Genome 48, 1070–1082. doi: 10.1139/g05-062

PubMed Abstract | CrossRef Full Text | Google Scholar

Sears, E. R. (1976). Genetic control of chromosome pairing in wheat. Annu. Rev. Genet. 10, 31–51. doi: 10.1146/annurev.ge.10.120176.000335

PubMed Abstract | CrossRef Full Text | Google Scholar

Shaked, H., Kashkush, K., Ozkan, H., Feldman, M., and Levy, A. A. (2001). Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. Plant Cell 13, 1749–1759. doi: 10.1105/tpc.13.8.1749

PubMed Abstract | CrossRef Full Text | Google Scholar

Song, K., Lu, P., Tang, K., and Osborn, T. C. (1995). Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. Proc. Natl. Acad. Sci. U.S.A. 92, 7719–7723. doi: 10.1073/pnas.92.17.7719

PubMed Abstract | CrossRef Full Text | Google Scholar

Spoor, W. (2001). Zohary, D., Hopf, M. 2000. Domestication of plants in the Old World. 3rd edn. 316pp. New York: Oxford University Press. £19.95 (softback). Ann. Bot. 88, 666. doi: 10.1006/anbo.2001.1505

CrossRef Full Text

Stevens, J. B., Abdallah, B. Y., Liu, G., Ye, C. J., Horne, S. D., Wang, G., et al. (2011). Diverse system stresses: common mechanisms of chromosome fragmentation. Cell Death Dis. 2, e178. doi: 10.1038/cddis.2011.60

PubMed Abstract | CrossRef Full Text | Google Scholar

Wojciechowska, B., and Pudelska, H. (2002). Production and morphology of the hybrids Aegilmops kotschyi x Secale cereale and Ae. biuncialis x S. cereale. J. Appl. Genet. 43, 279–285.

PubMed Abstract | Google Scholar

Wojciechowska, B., and Pudelska, H. (2005). Production and characterization of amphiploids of Aegilops kotschyi and Ae. biuncialis with Secale cereale, and of backcross hybrids of Ae. biuncialis x S. cereale amphiploids with 2x and 4x S. cereale. J. Appl. Genet. 46, 157–161.

PubMed Abstract | Google Scholar

Zickler, D., and Kleckner, N. (1999). Meiotic chromosomes: integrating structure and function. Annu. Rev. Genet. 33, 603–754. doi: 10.1146/annurev.genet.33.1.603

PubMed Abstract | CrossRef Full Text | Google Scholar

Zohary, D. (1999). Monophyletic vs. polyphyletic origin of the crops on which agriculture was founded in the Near East. Genet. Resour. Crop Evol. 46, 133–142. doi: 10.1023/A:1008692912820

CrossRef Full Text | Google Scholar

Zohary, D., and Feldman, M. (1962). Hybridization between amphidiploids and the evolution of polyploids in the wheat (Aegilops-triticum) group. Evolution 16, 44–61. doi: 10.1111/j.1558-5646.1962.tb03197.x

CrossRef Full Text | Google Scholar

Zwierzykowski, Z., Zwierzykowska, E., Taciak, M., Jones, N., Kosmala, A., and Krajewski, P. (2008). Chromosome pairing in allotetraploid hybrids of Festuca pratensis × Lolium perenne revealed by genomic in situ hybridization (GISH). Chromosome Res. 16, 575–585. doi: 10.1007/s10577-008-1198-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: allopolyploids, chromosome recombination, evolution, meiosis, mitosis, pivotal-differential theory, triticeae

Citation: Kwiatek MT, Majka J, Majka M, Belter J and Wisniewska H (2017) Adaptation of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe. Front. Plant Sci. 8:1300. doi: 10.3389/fpls.2017.01300

Received: 25 May 2017; Accepted: 10 July 2017;
Published: 25 July 2017.

Edited by:

Changbin Chen, University of Minnesota, United States

Reviewed by:

Kai Wang, Fujian Agriculture and Forestry University, China
Isabelle Colas, James Hutton Institute, United Kingdom

Copyright © 2017 Kwiatek, Majka, Majka, Belter and Wisniewska. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Michal T. Kwiatek, mkwi@igr.poznan.pl