Original Research ARTICLE

Front. Syst. Neurosci., 08 February 2010 |

Three-dimensional reconstruction and segmentation of intact Drosophila by ultramicroscopy

Department of Bioelectronics, FKE, Vienna University of Technology, Vienna, Austria
Bioelectronics, Center for Brain Research, Medical University of Vienna, Vienna, Austria
Department of Neurobiology, University of Oldenburg, Oldenburg, Germany
Max-Planck-Institute of Biochemistry, Muscle Dynamics, Martinsried, Germany
Genetic mutants are invaluable for understanding the development, physiology and behaviour of Drosophila. Modern molecular genetic techniques enable the rapid generation of large numbers of different mutants. To phenotype these mutants sophisticated microscopy techniques are required, ideally allowing the 3D-reconstruction of the anatomy of an adult fly from a single scan. Ultramicroscopy enables up to cm fields of view, whilst providing micron resolution. In this paper, we present ultramicroscopy reconstructions of the flight musculature, the nervous system, and the digestive tract of entire, chemically cleared, drosophila in autofluorescent light. The 3D-reconstructions thus obtained verify that the anatomy of a whole fly, including the filigree spatial organization of the direct flight muscles, can be analysed from a single ultramicroscopy reconstruction. The recording procedure, including 3D-reconstruction using standard software, takes no longer than 30 min. Additionally, image segmentation, which would allow for further quantitative analysis, was performed.
ultramicroscopy, imaging, Drosophila, segmentation, light sheet microscopy, morphology, phenotyping, flight muscle
Jährling N, Becker K, Schönbauer C, Schnorrer F and Dodt H-U (2010). Three-dimensional reconstruction and segmentation of intact Drosophila by ultramicroscopy. Front. Syst. Neurosci. 4:1. doi: 10.3389/neuro.06.001.2010
04 September 2009;
 Paper pending published:
29 October 2009;
13 January 2010;
 Published online:
08 February 2010.

Edited by:

Randolf Menzel, Freie Universität Berlin, Germany

Reviewed by:

Jean-Christophe Sandoz, CNRS University Paul Sabatier, France
© 2010 Jährling, Becker, Schönbauer, Schnorrer and Dodt. This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.
Nina Jährling, Center for Brain Research, Department of Bioelectronics, Spitalgasse 4, 1090 Vienna, Austria. e-mail: