AUTHOR=Yasuda Akikazu , Jones Leslie S., Shigeri Yasushi TITLE=The Multiplicity of Post-Translational Modifications in Pro-Opiomelanocortin-Derived Peptides JOURNAL=Frontiers in Endocrinology VOLUME=4 YEAR=2013 URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2013.00186 DOI=10.3389/fendo.2013.00186 ISSN=1664-2392 ABSTRACT=

The precursor protein, pro-opiomelanocortin (POMC) undergoes extensive post-translational processing in a tissue-specific manner to yield various biologically active peptides involved in diverse cellular functions. The recently developed method of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for direct tissue analysis has proved to be a powerful tool for investigating the distribution of peptides and proteins. In particular, topological mass spectrometry analysis using MALDI-MS can selectively provide a mass profile of the hormones included in cell secretory granules. An advantage of this technology is that it is possible to analyze a frozen thin slice section, avoiding an extraction procedure. Subsequently, tandem mass spectrometry (MS/MS) has a profound impact on addressing the modified residues in the hormone molecules. Based on these strategies with mass spectrometry, several interesting molecular forms of POMC-derived peptides have been found in the fish pituitary, such as novel sites of acetylation in α-melanocyte-stimulating hormone (MSH), hydroxylation of a proline residue in β-MSH, and the phosphorylated form of corticotropin-like intermediate lobe peptide.