AUTHOR=Sompallae Ramakrishna , Hofmann Oliver M., Maher Christopher A., Gedye Craig , Behren Andreas , Vitezic Morana , Daub Carsten O., Devalle Sylvie , Caballero Otavia L., Carninci Piero , Hayashizaki Yoshihide , Lawlor Elizabeth R., Cebon Jonathan , Hide Winston 

TITLE=A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers, and stem cells

JOURNAL=Frontiers in Genetics

VOLUME=4

YEAR=2013

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2013.00209

DOI=10.3389/fgene.2013.00209

ISSN=1664-8021

ABSTRACT=<p>PROM1 is the gene encoding prominin-1 or CD133, an important cell surface marker for the isolation of both normal and cancer stem cells. PROM1 transcripts initiate at a range of transcription start sites (TSS) associated with distinct tissue and cancer expression profiles. Using high resolution Cap Analysis of Gene Expression (CAGE) sequencing we characterize TSS utilization across a broad range of normal and developmental tissues. We identify a novel proximal promoter (P6) within CD133<sup>+</sup> melanoma cell lines and stem cells. Additional exon array sampling finds P6 to be active in populations enriched for mesenchyme, neural stem cells and within CD133<sup>+</sup> enriched Ewing sarcomas. The P6 promoter is enriched with respect to previously characterized PROM1 promoters for a HMGI/Y (HMGA1) family transcription factor binding site motif and exhibits different epigenetic modifications relative to the canonical promoter region of PROM1.</p>