AUTHOR=Holodick Nichol E. , Zeumer Leilani , Rothstein Thomas L. , Morel Laurence 

TITLE=Expansion of B-1a Cells with Germline Heavy Chain Sequence in Lupus Mice

JOURNAL=Frontiers in Immunology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2016.00108

DOI=10.3389/fimmu.2016.00108

ISSN=1664-3224

ABSTRACT=<p>B6.<italic>Sle1.Sle2.Sle3</italic> (B6.TC) lupus-prone mice carrying the NZB allele of <italic>Cdkn2c</italic>, encoding for the cyclin-dependent kinase inhibitor P18<sup>INK4</sup>, accumulate B-1a cells due to a higher rate of proliferative self-renewal. However, it is unclear whether this affects primarily early-appearing B-1a cells of fetal origin or later-appearing B-1a cells that emerge from bone marrow. B-1a cells are the major source of natural autoantibodies, and it has been shown that their protective nature is associated with a germline-like sequence, which is characterized by few N-nucleotide insertions and a repertoire skewed toward rearrangements predominated during fetal life, V<sub>H</sub>11 and V<sub>H</sub>12. To determine the nature of B-1a cells expanded in B6.TC mice, we amplified immunoglobulin genes by PCR from single cells in mice. Sequencing showed a significantly higher proportion of B-1a cell antibodies that display fewer N-additions in B6.TC mice than in B6 control mice. Following this lower number of N-insertions within the CDR-H3 region, the B6.TC B-1a cells display shorter CDR-H3 length than B6 B-1a cells. The absence of N-additions is a surrogate for fetal origin, as TdT expression starts after birth in mice. Therefore, our results suggest that the B-1a cell population is not only expanded in autoimmune B6.TC mice but also qualitatively different with the majority of cells from fetal origin. Accordingly, our sequencing results also demonstrated the overuse of V<sub>H</sub>11 and V<sub>H</sub>12 in autoimmune B6.TC mice as compared to B6 controls. These results suggest that the development of lupus autoantibodies in these mice is coupled with skewing of the B-1a cell repertoire and possible retention of protective natural antibodies.</p>