AUTHOR=Chandran P. Anoop , Heidu Sonja , Zelba Henning , Schmid-Horch Barbara , Rammensee Hans-Georg , Pascolo Steve , Gouttefangeas Cécile 

TITLE=A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry

JOURNAL=Frontiers in Immunology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2017.00096

DOI=10.3389/fimmu.2017.00096

ISSN=1664-3224

ABSTRACT=<p>Major histocompatibility complex (MHC) multimers are essential tools in T cell immunomonitoring, which are employed both in basic and clinical research, as well as for assessing clinical samples during therapy. The generation of MHC monomers loaded with synthetic peptides is an elaborate and time-consuming process. It would be beneficial to assess the quality of these monomers prior to downstream applications. In this technical note, we describe a novel flow cytometry-based, cell-free, quick, and robust assay to check the quality of MHC monomers directly after refolding or after long-term storage.</p>