AUTHOR=Hedlund Brian P. , Reysenbach Anna-Louise , Huang Liuquin , Ong John C. , Liu Zizhang , Dodsworth Jeremy A. , Ahmed Reham , Williams Amanda J. , Briggs Brandon R. , Liu Yitai , Hou Weiguo , Dong Hailiang 

TITLE=Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00157

DOI=10.3389/fmicb.2015.00157

ISSN=1664-302X

ABSTRACT=<p>The order <italic>Aquificales</italic> (phylum <italic>Aquificae</italic>) consists of thermophilic and hyperthermophilic bacteria that are prominent in many geothermal systems, including those in Tengchong, Yunnan Province, China. However, <italic>Aquificales</italic> have not previously been isolated from Tengchong. We isolated five strains of <italic>Aquificales</italic> from diverse springs (temperature 45.2–83.3°C and pH 2.6–9.1) in the Rehai Geothermal Field from sites in which <italic>Aquificales</italic> were abundant. Phylogenetic analysis showed that four of the strains belong to the genera <italic>Hydrogenobacter</italic>, <italic>Hydrogenobaculum</italic>, and <italic>Sulfurihydrogenibium</italic>, including strains distant enough to likely justify new species of <italic>Hydrogenobacter</italic> and <italic>Hydrogenobaculum</italic>. The additional strain may represent a new genus in the <italic>Hydrogenothermaceae</italic>. All strains were capable of aerobic respiration under microaerophilic conditions; however, they had variable capacity for chemolithotrophic oxidation of hydrogen and sulfur compounds and nitrate reduction.</p>