AUTHOR=Chen Zhenhong , Li Hongxia , Feng Jiao , Li Yuxue , Chen Xin , Guo Xuemin , Chen Weijun , Wang Li , Lin Lei , Yang Huiying , Yang Wenhui , Wang Jie , Zhou Dongsheng , Liu Changting , Yin Zhe 

TITLE=NDM-1 encoded by a pNDM-BJ01-like plasmid p3SP-NDM in clinical Enterobacter aerogenes

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00294

DOI=10.3389/fmicb.2015.00294

ISSN=1664-302X

ABSTRACT=<p>A carbapenem-nonsusceptible <italic>Enterobacter aerogenes</italic> strain named 3-SP was isolated from a human case of pneumonia in a Chinese teaching hospital. NDM-1 carbapenemase is produced by a pNDM-BJ01-like conjugative plasmid designated p3SP-NDM to account for carbapenem resistance of 3-SP. p3SP-NDM was fully sequenced and compared with all publically available pNDM-BJ01-like plasmids. The genetic differences between p3SP-NDM and pNDM-BJ01 include only 18 single nucleotide polymorphisms, a 1 bp deletion and a 706 bp deletion. p3SP-NDM and pNDM-BJ01 harbor an identical Tn<italic>125</italic> element organized as IS<italic>Aba125, bla</italic><sub>NDM−1</sub>, <italic>ble</italic><sub>MBL</sub>, Δ<italic>trpF, dsbC, cutA</italic>, Δ<italic>groES, groEL, ISCR27</italic>, and IS<italic>Aba125</italic>. The <italic>bla</italic><sub>NDM−1</sub> surrounding regions in these pNDM-BJ01-like plasmids have a conserved linear organization IS<italic>Aba14-<italic>aphA6</italic>-Tn<italic>125</italic>-unknown</italic> IS, with considerable genetic differences identified within or immediately downstream of Tn<italic>125</italic>. All reported pNDM-BJ01-like plasmids are exclusively found in <italic>Acinetobacter</italic>, whereas this is the first report of identification of a pNDM-BJ01-like plasmid in <italic>Enterobacteriaceae</italic>.</p>