AUTHOR=Sun Fengjun , Yin Zhe , Feng Jiao , Qiu Yefeng , Zhang Defu , Luo Wenbo , Yang Huiying , Yang Wenhui , Wang Jie , Chen Weijun , Xia Peiyuan , Zhou Dongsheng 

TITLE=Production of plasmid-encoding NDM-1 in clinical Raoultella ornithinolytica and Leclercia adecarboxylata from China

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00458

DOI=10.3389/fmicb.2015.00458

ISSN=1664-302X

ABSTRACT=<p><italic>Raoultella ornithinolytica</italic> YNKP001 and <italic>Leclercia adecarboxylata</italic> P10164, which harbor conjugative plasmids pYNKP001-NDM and pP10164-NDM, respectively, were isolated from two different Chinese patients, and their complete nucleotide sequences were determined. Production of NDM-1 enzyme by these plasmids accounts for the carbapenem resistance of these two strains. This is the first report of <italic>bla</italic><sub>NDM</sub> in <italic>L. adecarboxylata</italic> and third report of this gene in <italic>R. ornithinolytica</italic>. pYNKP001-NDM is very similar to the IncN2 NDM-1-encoding plasmids pTR3, pNDM-ECS01, and p271A, whereas pP10164-NDM is similar to the IncFII<sub>Y</sub><italic>bla</italic><sub>NDM-1</sub>-carrying plasmid pKOX_NDM1. The <italic>bla</italic><sub>NDM-1</sub> genes of pYNKP001-NDM and pP10164-NDM are embedded in Tn<italic>125</italic>-like elements, which represent two distinct truncated versions of the NDM-1-encoding Tn<italic>125</italic> prototype observed in pNDM-BJ01. Flanking of these two Tn<italic>125</italic>-like elements by miniature inverted repeat element (MITE) or its remnant indicates that MITE facilitates transposition and mobilization of <italic>bla</italic><sub>NDM-1</sub> gene contexts.</p>