AUTHOR=Kwiatos Natalia , Ryngajłło Małgorzata , Bielecki Stanisław 

TITLE=Diversity of laccase-coding genes in Fusarium oxysporum genomes

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00933

DOI=10.3389/fmicb.2015.00933

ISSN=1664-302X

ABSTRACT=<p>Multiple studies confirm laccase role in fungal pathogenicity and lignocellulose degradation. In spite of broad genomic research, laccases from plant wilt pathogen <italic>Fusarium oxysporum</italic> are still not characterized. The study aimed to identify <italic>F. oxysporum</italic> genes that may encode laccases <italic>sensu stricto</italic> and to characterize the proteins <italic>in silico</italic> in order to facilitate further research on their impact on the mentioned processes. Twelve sequenced <italic>F. oxysporum</italic> genomes available on <xref ref-type="bibr" rid="B6">Broad Institute of Harvard and MIT (2015)</xref> website were analyzed and three genes that may encode laccases <italic>sensu stricto</italic> were found. Their amino acid sequences possess all features essential for their catalytic activity, moreover, the homology models proved the characteristic 3D laccase structures. The study shades light on <italic>F. oxysporum</italic> as a new source of multicopper oxidases, enzymes with possible high redox potential and broad perspective in biotechnological applications.</p>