AUTHOR=Xu Zengping , Gao Yuxiang , Meng Shuai , Yang Baochen , Pang Liyun , Wang Chen , Liu Tianjun 

TITLE=Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00242

DOI=10.3389/fmicb.2016.00242

ISSN=1664-302X

ABSTRACT=<p>Lysine-porphyrin conjugate 4i has potent photosensitive antibacterial effect on clinical isolated bacterial strains such as Methicillin-resistant <italic>Staphylococcus aureus</italic> (MRSA), <italic>Escherichia coli</italic>, and <italic>Pseudomonas aeruginosa</italic>. The mechanism of photodynamic antibacterial chemotherapy of 4i (4i-PACT) <italic>in vitro</italic> and the treatment effect <italic>in vivo</italic> was investigated in this paper. Atomic force microscopy (AFM) revealed that 4i-PACT can effectively destroy membrane and wall of bacteria, resulting in leakage of its content. This was confirmed by dual fluorescent staining with acridine orange/ethidium bromide and measuring materials absorption at 260 nm. Agarose gel electrophoresis measurement showed that 4i-PACT can damage genomic DNA. Healing of wound in rat infected by mixed bacteria showed that the efficiency of 4i-PACT is dependent on the dose of light. These results showed that 4i-PACT has promising bactericidal effect both <italic>in vitro</italic> and <italic>in vivo</italic>.</p>