AUTHOR=Wang He , Chen Ya-Lei , Teng Shih-Hua , Xu Zhi-Peng , Xu Ying-Chun , Hsueh Po-Ren 

TITLE=Evaluation of the Bruker Biotyper Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry System for Identification of Clinical and Environmental Isolates of Burkholderia pseudomallei

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00415

DOI=10.3389/fmicb.2016.00415

ISSN=1664-302X

ABSTRACT=<p><italic>Burkholderia pseudomallei</italic> is not represented in the current version of Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. A total of 66 isolates of <italic>B. pseudomallei</italic>, including 30 clinical isolates collected from National Taiwan University Hospital (NTUH, <italic>n</italic> = 27) and Peking Union Medical College Hospital (PUMCH, <italic>n</italic> = 3), and 36 isolates of genetically confirmed strains, including 13 from clinical samples and 23 from environmental samples, collected from southern Taiwan were included in this study. All these isolates were identified by partial 16S rDNA gene sequencing analysis and the Bruker Biotyper MALDI-TOF MS system. Among the 30 isolates initially identified as <italic>B. pseudomallei</italic> by conventional identification methods, one was identified as <italic>B. cepacia</italic> complex (NTUH) and three were identified as <italic>B. putida</italic> (PUMCH) by partial 16S rDNA gene sequencing analysis and Bruker Biotyper MALDI-TOF MS system. The Bruker Biotyper MALDI-TOF MS system misidentified 62 genetically confirmed <italic>B. pseudomallei</italic> isolates as <italic>B. thailandensis</italic> or <italic>Burkholderia</italic> species (score values, 1.803–2.063) when the currently available database (DB 5627) was used. However, using a newly created MALDI-TOF MS database (including <italic>B. pseudomallei</italic> NTUH-3 strain), all isolates were correctly identified as <italic>B. pseudomallei</italic> (score values &gt;2.000, 100%). An additional 60 isolates of genetically confirmed <italic>B. cepacia</italic> complex and <italic>B. putida</italic> were also evaluated by the Bruker Biotyper MALDI-TOF MS system using the newly created database and none of these isolates were identified as <italic>B. pseudomallei</italic>. MALDI-TOF MS is a versatile and robust tool for the rapid identification of <italic>B. pseudomallei</italic> using the enhanced database.</p>