AUTHOR=Merhej Jawad , Thiebaut Antonin , Blugeon Corinne , Pouch Juliette , Ali Chaouche Mohammed El Amine , Camadro Jean-Michel , Le Crom Stéphane , Lelandais Gaëlle , Devaux Frédéric 

TITLE=A Network of Paralogous Stress Response Transcription Factors in the Human Pathogen Candida glabrata

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00645

DOI=10.3389/fmicb.2016.00645

ISSN=1664-302X

ABSTRACT=<p>The yeast <italic>Candida glabrata</italic> has become the second cause of systemic candidemia in humans. However, relatively few genome-wide studies have been conducted in this organism and our knowledge of its transcriptional regulatory network is quite limited. In the present work, we combined genome-wide chromatin immunoprecipitation (ChIP-seq), transcriptome analyses, and DNA binding motif predictions to describe the regulatory interactions of the seven Yap (Yeast AP1) transcription factors of <italic>C. glabrata</italic>. We described a transcriptional network containing 255 regulatory interactions and 309 potential target genes. We predicted with high confidence the preferred DNA binding sites for 5 of the 7 CgYaps and showed a strong conservation of the Yap DNA binding properties between <italic>S. cerevisiae</italic> and <italic>C. glabrata</italic>. We provided reliable functional annotation for 3 of the 7 Yaps and identified for Yap1 and Yap5 a core regulon which is conserved in <italic>S. cerevisiae, C. glabrata</italic>, and <italic>C. albicans</italic>. We uncovered new roles for CgYap7 in the regulation of iron-sulfur cluster biogenesis, for CgYap1 in the regulation of heme biosynthesis and for CgYap5 in the repression of <italic>GRX4</italic> in response to iron starvation. These transcription factors define an interconnected transcriptional network at the cross-roads between redox homeostasis, oxygen consumption, and iron metabolism.</p>