AUTHOR=Weng Yuding , Chen Fei , Liu Yiwei , Zhao Qiang , Chen Ronghao , Pan Xiaolei , Liu Chang , Cheng Zhihui , Jin Shouguang , Jin Yongxin , Wu Weihui 

TITLE=Pseudomonas aeruginosa Enolase Influences Bacterial Tolerance to Oxidative Stresses and Virulence

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.01999

DOI=10.3389/fmicb.2016.01999

ISSN=1664-302X

ABSTRACT=<p><italic>Pseudomonas aeruginosa</italic> is a Gram negative opportunistic pathogenic bacterium, which causes acute and chronic infections. Upon entering the host, bacteria alter global gene expression to adapt to host environment and avoid clearance by the host. Enolase is a glycolytic enzyme involved in carbon metabolism. It is also a component of RNA degradosome, which is involved in RNA processing and gene regulation. Here, we report that enolase is required for the virulence of <italic>P. aeruginosa</italic> in a murine acute pneumonia model. Mutation of enolase coding gene (<italic>eno</italic>) increased bacterial susceptibility to neutrophil mediated killing, which is due to reduced tolerance to oxidative stress. Catalases and alkyl hydroperoxide reductases play a major role in protecting the cell from oxidative damages. In the <italic>eno</italic> mutant, the expression levels of catalases (KatA and KatB) were similar as those in the wild type strain in the presence of H<sub>2</sub>O<sub>2</sub>, however, the expression levels of alkyl hydroperoxide reductases (AhpB and AhpC) were significantly reduced. Overexpression of <italic>ahpB</italic> but not <italic>ahpC</italic> in the <italic>eno</italic> mutant fully restored the bacterial resistance to H<sub>2</sub>O<sub>2</sub> as well as neutrophil mediated killing, and partially restored bacterial virulence in the murine acute pneumonia model. Therefore, we have identified a novel role of enolase in the virulence of <italic>P. aeruginosa</italic>.</p>