AUTHOR=Massart Sebastien , Candresse Thierry , Gil José , Lacomme Christophe , Predajna Lukas , Ravnikar Maja , Reynard Jean-Sébastien , Rumbou Artemis , Saldarelli Pasquale , Škorić Dijana , Vainio Eeva J. , Valkonen Jari P. T. , Vanderschuren Hervé , Varveri Christina , Wetzel Thierry TITLE=A Framework for the Evaluation of Biosecurity, Commercial, Regulatory, and Scientific Impacts of Plant Viruses and Viroids Identified by NGS Technologies JOURNAL=Frontiers in Microbiology VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.00045 DOI=10.3389/fmicb.2017.00045 ISSN=1664-302X ABSTRACT=

Recent advances in high-throughput sequencing technologies and bioinformatics have generated huge new opportunities for discovering and diagnosing plant viruses and viroids. Plant virology has undoubtedly benefited from these new methodologies, but at the same time, faces now substantial bottlenecks, namely the biological characterization of the newly discovered viruses and the analysis of their impact at the biosecurity, commercial, regulatory, and scientific levels. This paper proposes a scaled and progressive scientific framework for efficient biological characterization and risk assessment when a previously known or a new plant virus is detected by next generation sequencing (NGS) technologies. Four case studies are also presented to illustrate the need for such a framework, and to discuss the scenarios.