AUTHOR=Wang Lei , Sun Ximeng , Huang Jingjing , Zhan Bin , Zhu Xinping 

TITLE=Heterologous Prime-Boost Vaccination Enhances TsPmy’s Protective Immunity against Trichinella spiralis Infection in a Murine Model

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.01394

DOI=10.3389/fmicb.2017.01394

ISSN=1664-302X

ABSTRACT=<p><italic>Ts</italic>Pmy is a paramyosin expressed by parasitic <italic>Trichinella spiralis</italic> and confers a protective immunity when its recombinant protein or DNA was used as an immunogen. To improve its immunogenicity and vaccine efficacy, we conducted a heterologous prime-boost strategy by orally delivering one dose of <italic>Ts</italic>Pmy DNA carried by attenuated <italic>Salmonella typhimurium</italic> (SL7207), followed by two doses of recombinant <italic>Ts</italic>Pmy intramuscularly. This strategy effectively induced intestinal mucosal sIgA response and an enhanced and balanced Th1/Th2 immune responses that improve protection against <italic>T. spiralis</italic> larval challenge, with 55.4% muscle larvae reduction and 41.8% adult worm reduction compared to PBS control. The muscle larvae reduction induced by heterologous prime-boost regimen was significant higher than that induced by the homologous DNA or protein prime-boost regimens, which could act as a practical prophylactic approach to prevent <italic>T. spiralis</italic> infection.</p>