AUTHOR=Taylor Gordon T. , Suter Elizabeth A. , Li Zhuo Q. , Chow Stephanie , Stinton Dallyce , Zaliznyak Tatiana , Beaupré Steven R. 

TITLE=Single-Cell Growth Rates in Photoautotrophic Populations Measured by Stable Isotope Probing and Resonance Raman Microspectrometry

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.01449

DOI=10.3389/fmicb.2017.01449

ISSN=1664-302X

ABSTRACT=<p>A new method to measure growth rates of individual photoautotrophic cells by combining stable isotope probing (SIP) and single-cell resonance Raman microspectrometry is introduced. This report explores optimal experimental design and the theoretical underpinnings for quantitative responses of Raman spectra to cellular isotopic composition. Resonance Raman spectra of isogenic cultures of the cyanobacterium, <italic>Synechococcus</italic> sp., grown in <sup>13</sup>C-bicarbonate revealed linear covariance between wavenumber (cm<sup>−1</sup>) shifts in dominant carotenoid Raman peaks and a broad range of cellular <sup>13</sup>C fractional isotopic abundance. Single-cell growth rates were calculated from spectra-derived isotopic content and empirical relationships. Growth rates among any 25 cells in a sample varied considerably; mean coefficient of variation, CV, was 29 ± 3% (σ/<inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mover accent="false" class="mml-overline"><mml:mrow><mml:mi>x</mml:mi></mml:mrow><mml:mo accent="true">¯</mml:mo></mml:mover></mml:math></inline-formula>), of which only ~2% was propagated analytical error. Instantaneous population growth rates measured independently by <italic>in vivo</italic> fluorescence also varied daily (CV ≈ 53%) and were statistically indistinguishable from single-cell growth rates at all but the lowest levels of cell labeling. SCRR censuses of mixtures prepared from <italic>Synechococcus</italic> sp. and <italic>T. pseudonana</italic> (a diatom) populations with varying <sup>13</sup>C-content and growth rates closely approximated predicted spectral responses and fractional labeling of cells added to the sample. This approach enables direct microspectrometric interrogation of isotopically- and phylogenetically-labeled cells and detects as little as 3% changes in cellular fractional labeling. This is the first description of a non-destructive technique to measure single-cell photoautotrophic growth rates based on Raman spectroscopy and well-constrained assumptions, while requiring few ancillary measurements.</p>