AUTHOR=Ramírez-Castrillón Mauricio , Jaramillo-Garcia Victoria P. , Rosa Priscila D. , Landell Melissa F. , Vu Duong , Fabricio Mariana F. , Ayub Marco A. Z. , Robert Vincent , Henriques João A. P. , Valente Patricia 

TITLE=The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.01776

DOI=10.3389/fmicb.2017.01776

ISSN=1664-302X

ABSTRACT=<p>A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast, <italic>Meyerozyma guilliermondii</italic> BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38% for glucose or 34.97% for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a biodiesel refinery. The lipid profile showed monounsaturated fatty acids (MUFA) varying between 56 or 74% in pure or raw glycerol, respectively. <italic>M. guilliermondii</italic> BI281A bears potential as a new biodiesel feedstock.</p>