AUTHOR=Spath Cathleen , Schlegel Franziska , Leontyev Sergey , Mohr Friedrich W., Dhein Stefan 

TITLE=Inverse Relationship between Tumor Proliferation Markers and Connexin Expression in a Malignant Cardiac Tumor Originating from Mesenchymal Stem Cell Engineered Tissue in a Rat in vivo Model

JOURNAL=Frontiers in Pharmacology

VOLUME=4

YEAR=2013

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2013.00042

DOI=10.3389/fphar.2013.00042

ISSN=1663-9812

ABSTRACT=<p><bold>Background:</bold> Recently, we demonstrated the beneficial effects of engineered heart tissues for the treatment of dilated cardiomyopathy in rats. For further development of this technique we started to produce engineered tissue (ET) from mesenchymal stem cells. Interestingly, we observed a malignant tumor invading the heart with an inverse relationship between proliferation markers and connexin expression.</p><p><bold>Methods:</bold> Commercial CD54<sup>+</sup>/CD90<sup>+</sup>/CD34<sup>−</sup>/CD45<sup>−</sup> bone marrow derived mesenchymal rat stem cells (cBM-MSC), characterized were used for production of mesenchymal stem-cell-ET (MSC-ET) by suspending them in a collagen I, matrigel-mixture and cultivating for 14 days with electrical stimulation. Three MSC-ET were implanted around the beating heart of adult rats for days. Another three MSC-ET were produced from freshly isolated rat bone marrow derived stem cells (sBM-MSC).</p><p><bold>Results:</bold> Three weeks after implantation of the MSC-ETs the hearts were surgically excised. While in 5/6 cases the ET was clearly distinguishable and was found as a ring containing mostly connective tissue around the heart, in 1/6 the heart was completely surrounded by a huge, undifferentiated, pleomorphic tumor originating from the cMSC-ET (cBM-MSC), classified as a high grade malignant sarcoma. Quantitatively we found a clear inverse relationship between cardiac connexin expression (Cx43, Cx40, or Cx45) and increased Ki-67 expression (Cx43: <italic>p</italic> &lt; 0.0001, Cx45: <italic>p</italic> &lt; 0.03, Cx40: <italic>p</italic> &lt; 0.014). At the tumor-heart border there were significantly more Ki-67 positive cells (<italic>p</italic> = 0.001), and only 2% Cx45 and Ki-67-expressing cells, while the other connexins were nearly completely absent (<italic>p</italic> &lt; 0.0001).</p><p><bold>Conclusion and Hypothesis:</bold> These observations strongly suggest the hypothesis, that invasive tumor growth is accompanied by reduction in connexins. This implicates that gap junction communication between tumor and normal tissue is reduced or absent, which could mean that growth and differentiation signals can not be exchanged.</p>