AUTHOR=Baskaran Ponnusamy , Kumari Aloka , Ncube Bhekumthetho , Van Staden Johannes 

TITLE=Acetylcholinesterase-Inhibition and Antibacterial Activity of Mondia whitei Adventitious Roots and Ex vitro-Grown Somatic Embryogenic-Biomass

JOURNAL=Frontiers in Pharmacology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2016.00335

DOI=10.3389/fphar.2016.00335

ISSN=1663-9812

ABSTRACT=<p><italic>Mondia whitei</italic> (Hook.f.) Skeels is an important endangered medicinal and commercial plant in South Africa. <italic>In vitro</italic> propagation systems are required for biomass production and bioactivity analysis to supplement wild resources/stocks. Adventitious roots from somatic embryogenic explants using suspension culture and <italic>ex vitro</italic>-grown plants produced via somatic embryogenesis were established using different plant growth regulator treatments. The adventitious root biomass and different parts of <italic>ex vitro</italic>-grown and mother plants were used to investigate the potential for acetylcholinesterase (AChE) and antibacterial activities. Adventitious roots derived from 2.5 μM indole-3-acetic acid (IAA) treatments and <italic>ex vitro-</italic>grown plants derived from <italic>meta</italic>-topolin riboside and IAA treatments gave the best AChE and antibacterial activities. The <italic>in vitro-</italic>established <italic>M. whitei</italic> and <italic>ex vitro</italic> biomass have comparable ability to function as inhibitors of acetylcholinesterase and antibacterial agents, and can be used as potent bioresources in traditional medicine.</p>